Name: Amphibacillus iburiensis N314
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 133954

Type strain:

Species: Amphibacillus iburiensis

Strain Designation: N314

Culture col. no.: JCM 18529, NCIMB 14823

Strain history: I. Yumoto N314.

NCBI tax ID(s): 1212662 (species)

Special Collections

Section

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Amphibacillus iburiensis N314 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from indigo dye.

Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Amphibacillus*
	Species	**Amphibacillus iburiensis**
	Full Scientific Name (LPSN)	Amphibacillus iburiensis Hirota et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30941]	Gram stain	positive

[Ref.: #30941]	Cell length	2.35 µm

[Ref.: #30941]	Cell width	0.35 µm

[Ref.: #30941]	Cell shape	rod-shaped

[Ref.: #30941]	Motility	yes

[Ref.: #30941]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #30941]	growth	26-39 °C
[Ref.: #30941]	optimum	36 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #30941]			optimum	9
[Ref.: #30941]			growth	8.0-9.1

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30941]

Oxygen tolerance

facultative anaerobe

[Ref.: #30941]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30941]		NaCl		growth	0-7	%
[Ref.: #30941]		NaCl		optimum	1.5	%

[Ref.: #30941]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30941]	arabinose ChEBI	+	carbon source
[Ref.: #30941]	cellobiose ChEBI	+	carbon source
[Ref.: #30941]	fructose ChEBI	+	carbon source
[Ref.: #30941]	galactose ChEBI	+	carbon source
[Ref.: #30941]	glucose ChEBI	+	carbon source
[Ref.: #30941]	lactose ChEBI	+	carbon source
[Ref.: #30941]	mannose ChEBI	+	carbon source
[Ref.: #30941]	melibiose ChEBI	+	carbon source
[Ref.: #30941]	raffinose ChEBI	+	carbon source
[Ref.: #30941]	rhamnose ChEBI	+	carbon source
[Ref.: #30941]	sucrose ChEBI	+	carbon source
[Ref.: #30941]	trehalose ChEBI	+	carbon source
[Ref.: #30941]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30941]	acid phosphatase	+	3.1.3.2
[Ref.: #30941]	alkaline phosphatase	+	3.1.3.1
[Ref.: #30941]	alpha-galactosidase	+	3.2.1.22

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #30941]	Sample type/isolated from	indigo dye

[Ref.: #67770]	Sample type/isolated from	Fermented polygonum indigo (Polygonum tinctorium Lour.) liquor from Date City
[Ref.: #67770]	Host species	Polygonum tinctorium
[Ref.: #67770]	Geographic location (country and/or sea, region)	Iburi Branch, Hokkaido
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Engineered product

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #30941]	Amphibacillus iburiensis gene for 16S ribosomal RNA, partial sequence	AB736274	1526	GenBank ENA	1212662 tax ID

[Ref.: #30941]	GC-content	38.4 mol%
[Ref.: #67770]	GC-content	38.4 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #30941]

Culture collection no.

JCM 18529, NCIMB 14823

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Amphibacillus iburiensis sp. nov., an alkaliphile that reduces an indigo dye.	Hirota K, Aino K, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijs.0.048009-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30941

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27271 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#27271

IJSEM 4303 2013 ( DOI 10.1099/ijs.0.048009-0 , PubMed 23832971 )

* These data were automatically processed and therefore are not curated

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