Name: Flectobacillus fontis MIB-4
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 133330

Type strain:

Species: Flectobacillus fontis

Strain Designation: MIB-4

Culture col. no.: BCRC 80926, LMG 29292, KCTC 33763

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

NCBI tax ID(s): 1737445 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Flectobacillus fontis MIB-4 is an aerobe, chemoheterotroph, Gram-negative bacterium that forms circular colonies and was isolated from freshwater.

colony-forming
Gram-negative
rod-shaped
aerobe
chemoheterotroph
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacteroidota*
	Class	*Cytophagia*
	Order	*Cytophagales*
	Family	*Spirosomataceae*
	Genus	*Flectobacillus*
	Species	**Flectobacillus fontis**
	Full Scientific Name (LPSN)	Flectobacillus fontis Chen et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25183]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #25183]	Cell length	4.0-8.0 µm

[Ref.: #25183]	Cell width	0.5-0.7 µm

[Ref.: #25183]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #25183]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #25183]	Colony size	3.0-6.5 mm
[Ref.: #25183]	Colony color	pale pink
[Ref.: #25183]	Colony shape	circular
[Ref.: #25183]	Incubation period	2 days
[Ref.: #25183]	Cultivation medium used	R2A agar

[Ref.: #25183]	Pigment production	no
[Ref.: #25183]	Pigment name	Flexirubin-type
[Ref.: #25183]	Pigment production	yes
[Ref.: #25183]	Pigment name	arotenoid

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25183]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #25183]

Culture medium growth

		Temperature
[Ref.: #25183]	growth	15.0-30.0 °C
[Ref.: #25183]	optimum	25 °C
[Ref.: #67771]	growth	25 °C

	pH	Kind of pH		pH
[Ref.: #25183]			optimum	7
[Ref.: #25183]			growth	7.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25183]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #25183]

Nutrition type

chemoheterotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25183]		NaCl		growth	0.0-0.5	%
[Ref.: #25183]		NaCl		optimum	0	%

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25183]	(-)-quinic acid ChEBI	-	carbon source
[Ref.: #25183]	2,3-butanediol ChEBI	-	carbon source
[Ref.: #25183]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #25183]	2-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #25183]	2-oxobutanoate ChEBI	+	carbon source
[Ref.: #25183]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #25183]	2-oxopentanoate ChEBI	-	carbon source
[Ref.: #25183]	3-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #25183]	4-hydroxy-L-proline ChEBI	-	carbon source
[Ref.: #25183]	4-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #25183]	4-hydroxyphenylacetic acid ChEBI	+	carbon source
[Ref.: #25183]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #25183]	acetate ChEBI	-	carbon source
[Ref.: #25183]	adipate ChEBI	-	assimilation
[Ref.: #25183]	alpha-cyclodextrin ChEBI	+	carbon source
[Ref.: #25183]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #25183]	alpha-lactose ChEBI	-	carbon source
[Ref.: #25183]	amygdalin ChEBI	-	builds acid from
[Ref.: #25183]	arabinose ChEBI	-	assimilation
[Ref.: #25183]	arbutin ChEBI	-	builds acid from
[Ref.: #25183]	bromosuccinate ChEBI	-	carbon source
[Ref.: #25183]	butyrate ChEBI	-	assimilation
[Ref.: #25183]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #25183]	carnitine ChEBI	-	carbon source
[Ref.: #25183]	casein	-	hydrolysis
[Ref.: #25183]	cellobiose ChEBI	-	builds acid from
[Ref.: #25183]	cellobiose ChEBI	+	carbon source
[Ref.: #25183]	chitin ChEBI	-	hydrolysis
[Ref.: #25183]	cis-aconitate ChEBI	-	carbon source
[Ref.: #25183]	citrate ChEBI	-	assimilation
[Ref.: #25183]	citrate ChEBI	-	carbon source
[Ref.: #25183]	D-alanine ChEBI	+	carbon source
[Ref.: #25183]	D-arabinose ChEBI	-	builds acid from
[Ref.: #25183]	D-arabitol ChEBI	-	builds acid from
[Ref.: #25183]	D-arabitol ChEBI	-	carbon source
[Ref.: #25183]	D-fructose ChEBI	+	builds acid from
[Ref.: #25183]	D-fructose ChEBI	+	carbon source
[Ref.: #25183]	D-fucose ChEBI	-	builds acid from
[Ref.: #25183]	D-galactonic acid lactone ChEBI	-	carbon source
[Ref.: #25183]	D-galactose ChEBI	+	builds acid from
[Ref.: #25183]	D-galactose ChEBI	+	carbon source
[Ref.: #25183]	D-galacturonic acid ChEBI	-	carbon source
[Ref.: #25183]	D-glucarate ChEBI	-	carbon source
[Ref.: #25183]	D-gluconate ChEBI	-	carbon source
[Ref.: #25183]	D-glucosaminic acid ChEBI	-	carbon source
[Ref.: #25183]	D-glucose ChEBI	-	builds acid from
[Ref.: #25183]	D-glucose 6-phosphate ChEBI	-	carbon source
[Ref.: #25183]	D-glucuronate ChEBI	-	carbon source
[Ref.: #25183]	D-lyxose ChEBI	-	builds acid from
[Ref.: #25183]	D-mannitol ChEBI	-	builds acid from
[Ref.: #25183]	D-mannitol ChEBI	-	carbon source
[Ref.: #25183]	D-mannose ChEBI	-	builds acid from
[Ref.: #25183]	D-mannose ChEBI	+	carbon source
[Ref.: #25183]	D-psicose ChEBI	+	carbon source
[Ref.: #25183]	D-ribose ChEBI	+	builds acid from
[Ref.: #25183]	D-serine ChEBI	-	carbon source
[Ref.: #25183]	D-sorbitol ChEBI	-	carbon source
[Ref.: #25183]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25183]	D-tagatose ChEBI	-	builds acid from
[Ref.: #25183]	D-trehalose ChEBI	-	builds acid from
[Ref.: #25183]	D-xylose ChEBI	+	builds acid from
[Ref.: #25183]	dextrin ChEBI	+	carbon source
[Ref.: #25183]	dna ChEBI	+	hydrolysis
[Ref.: #25183]	erythritol ChEBI	-	builds acid from
[Ref.: #25183]	erythritol ChEBI	-	carbon source
[Ref.: #25183]	esculin ChEBI	+	hydrolysis
[Ref.: #25183]	esculin ChEBI	+	builds acid from
[Ref.: #25183]	ethanolamine ChEBI	-	carbon source
[Ref.: #25183]	formate ChEBI	-	carbon source
[Ref.: #25183]	galactitol ChEBI	-	builds acid from
[Ref.: #25183]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #25183]	gelatin ChEBI	-	hydrolysis
[Ref.: #25183]	gentiobiose ChEBI	+	builds acid from
[Ref.: #25183]	gentiobiose ChEBI	+	carbon source
[Ref.: #25183]	gluconate ChEBI	-	assimilation
[Ref.: #25183]	gluconate ChEBI	-	builds acid from
[Ref.: #25183]	glucose ChEBI	-	assimilation
[Ref.: #25183]	glucose 1-phosphate ChEBI	+	carbon source
[Ref.: #25183]	glucuronamide ChEBI	-	carbon source
[Ref.: #25183]	glycerol ChEBI	-	builds acid from
[Ref.: #25183]	glycerol ChEBI	-	carbon source
[Ref.: #25183]	glycerol 1-phosphate ChEBI	-	carbon source
[Ref.: #25183]	glycogen ChEBI	-	builds acid from
[Ref.: #25183]	glycogen ChEBI	+	carbon source
[Ref.: #25183]	glycyl L-aspartic acid ChEBI	-	carbon source
[Ref.: #25183]	glycyl-l-glutamate ChEBI	-	carbon source
[Ref.: #25183]	inosine ChEBI	-	carbon source
[Ref.: #25183]	inulin ChEBI	-	builds acid from
[Ref.: #25183]	itaconate ChEBI	-	carbon source
[Ref.: #25183]	L-alaninamide ChEBI	+	carbon source
[Ref.: #25183]	L-alanine ChEBI	-	carbon source
[Ref.: #25183]	L-alanylglycine ChEBI	+	carbon source
[Ref.: #25183]	L-arabinose ChEBI	-	carbon source
[Ref.: #25183]	L-arabinose ChEBI	+	builds acid from
[Ref.: #25183]	L-arabitol ChEBI	-	builds acid from
[Ref.: #25183]	L-asparagine ChEBI	-	carbon source
[Ref.: #25183]	L-aspartate ChEBI	-	carbon source
[Ref.: #25183]	L-fucose ChEBI	-	builds acid from
[Ref.: #25183]	L-fucose ChEBI	+	carbon source
[Ref.: #25183]	L-glutamate ChEBI	+	carbon source
[Ref.: #25183]	L-histidine ChEBI	-	carbon source
[Ref.: #25183]	L-leucine ChEBI	-	carbon source
[Ref.: #25183]	L-ornithine ChEBI	-	carbon source
[Ref.: #25183]	L-phenylalanine ChEBI	-	carbon source
[Ref.: #25183]	L-proline ChEBI	-	carbon source
[Ref.: #25183]	L-pyroglutamic acid ChEBI	-	carbon source
[Ref.: #25183]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #25183]	L-rhamnose ChEBI	-	carbon source
[Ref.: #25183]	L-serine ChEBI	-	carbon source
[Ref.: #25183]	L-sorbose ChEBI	-	builds acid from
[Ref.: #25183]	L-threonine ChEBI	-	carbon source
[Ref.: #25183]	L-xylose ChEBI	-	builds acid from
[Ref.: #25183]	lactate ChEBI	+	carbon source
[Ref.: #25183]	lactose ChEBI	+	builds acid from
[Ref.: #25183]	lactulose ChEBI	-	carbon source
[Ref.: #25183]	malate ChEBI	-	assimilation
[Ref.: #25183]	malonate ChEBI	-	carbon source
[Ref.: #25183]	maltose ChEBI	-	builds acid from
[Ref.: #25183]	maltose ChEBI	-	assimilation
[Ref.: #25183]	maltose ChEBI	+	carbon source
[Ref.: #25183]	mannitol ChEBI	-	assimilation
[Ref.: #25183]	mannose ChEBI	-	assimilation
[Ref.: #25183]	melezitose ChEBI	-	builds acid from
[Ref.: #25183]	melibiose ChEBI	+	builds acid from
[Ref.: #25183]	melibiose ChEBI	+	carbon source
[Ref.: #25183]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #25183]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #25183]	methyl beta-D-glucopyranoside ChEBI	+	carbon source
[Ref.: #25183]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #25183]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #25183]	monomethyl succinate ChEBI	+	carbon source
[Ref.: #25183]	myo-inositol ChEBI	-	builds acid from
[Ref.: #25183]	myo-inositol ChEBI	-	carbon source
[Ref.: #25183]	N-acetylgalactosamine ChEBI	+	carbon source
[Ref.: #25183]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #25183]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #25183]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #25183]	nitrate ChEBI	+	reduction
[Ref.: #25183]	phenylacetate ChEBI	-	assimilation
[Ref.: #25183]	phenylethylamine ChEBI	-	carbon source
[Ref.: #25183]	propionate ChEBI	-	carbon source
[Ref.: #25183]	putrescine ChEBI	-	carbon source
[Ref.: #25183]	raffinose ChEBI	+	builds acid from
[Ref.: #25183]	raffinose ChEBI	+	carbon source
[Ref.: #25183]	ribitol ChEBI	-	carbon source
[Ref.: #25183]	ribitol ChEBI	-	builds acid from
[Ref.: #25183]	salicin ChEBI	+	builds acid from
[Ref.: #25183]	sebacic acid ChEBI	-	carbon source
[Ref.: #25183]	starch ChEBI	-	builds acid from
[Ref.: #25183]	starch ChEBI	+	hydrolysis
[Ref.: #25183]	succinamate ChEBI	-	carbon source
[Ref.: #25183]	succinate ChEBI	-	carbon source
[Ref.: #25183]	sucrose ChEBI	-	builds acid from
[Ref.: #25183]	sucrose ChEBI	+	carbon source
[Ref.: #25183]	thymidine ChEBI	-	carbon source
[Ref.: #25183]	trehalose ChEBI	+	carbon source
[Ref.: #25183]	tryptophan ChEBI	-	energy source
[Ref.: #25183]	turanose ChEBI	-	builds acid from
[Ref.: #25183]	turanose ChEBI	+	carbon source
[Ref.: #25183]	tween 20 ChEBI	-	hydrolysis
[Ref.: #25183]	tween 40 ChEBI	-	hydrolysis
[Ref.: #25183]	tween 40 ChEBI	-	carbon source
[Ref.: #25183]	tween 60 ChEBI	-	hydrolysis
[Ref.: #25183]	tween 80 ChEBI	-	hydrolysis
[Ref.: #25183]	tween 80 ChEBI	-	carbon source
[Ref.: #25183]	urea ChEBI	-	hydrolysis
[Ref.: #25183]	uridine ChEBI	+	carbon source
[Ref.: #25183]	urocanic acid ChEBI	-	carbon source
[Ref.: #25183]	xylitol ChEBI	-	builds acid from
[Ref.: #25183]	xylitol ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #25183]	ampicillin ChEBI	yes	10	µg
[Ref.: #25183]	chloramphenicol ChEBI	yes	30	µg
[Ref.: #25183]	gentamicin ChEBI	yes	10	µg
[Ref.: #25183]	kanamycin ChEBI	yes	30	µg
[Ref.: #25183]	nalidixic acid ChEBI	yes	30	µg
[Ref.: #25183]	novobiocin ChEBI	yes	30	µg
[Ref.: #25183]	penicillin g ChEBI	yes	10	Unit
[Ref.: #25183]	rifampicin ChEBI	yes	5	µg
[Ref.: #25183]	streptomycin ChEBI	yes	10	µg
[Ref.: #25183]	tetracycline ChEBI	yes	30	µg

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #25183]		26	sulfamethoxazole ChEBI	yes	23.75	µg
[Ref.: #25183]		26	trimethoprim ChEBI	yes	1.25	µg

	Metabolite production	Metabolite	Production
[Ref.: #25183]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test
[Ref.: #25183]		indole ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #25183]	acid phosphatase	+	3.1.3.2
[Ref.: #25183]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25183]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #25183]	alpha-fucosidase	+	3.2.1.51
[Ref.: #25183]	alpha-galactosidase	+	3.2.1.22
[Ref.: #25183]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25183]	alpha-mannosidase	+	3.2.1.24
[Ref.: #25183]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25183]	beta-galactosidase	+	3.2.1.23
[Ref.: #25183]	beta-glucosidase	+	3.2.1.21
[Ref.: #25183]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25183]	catalase	+	1.11.1.6
[Ref.: #25183]	cystine arylamidase	-	3.4.11.3
[Ref.: #25183]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25183]	esterase (C 4)	-
[Ref.: #25183]	esterase Lipase (C 8)	+
[Ref.: #25183]	leucine arylamidase	+	3.4.11.1
[Ref.: #25183]	lipase	-
[Ref.: #25183]	lipase (C 14)	-
[Ref.: #25183]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #25183]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25183]	trypsin	-	3.4.21.4
[Ref.: #25183]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25183]	urease	-	3.5.1.5
[Ref.: #25183]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25183]

Fatty acid	Percentage
C16:1ω5c	31.6
C15:0 iso	16.6
C16:1ω7c / C16:1ω6c	14.8
C17:0 iso 3OH	6.9
C15:0 iso 3OH	6.7
C15:1ω6c	3.6
C16:0 3OH	3.6
C16:0	3.3
C17:1 iso I /C17:1 anteiso B	2.4
C15:0 anteiso	2.1
C17:0 iso	1.7
C16:0 2OH	1.4
C14:0	1.4
C14:0 3OH / C16:1 iso I	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25183]	Sample type/isolated from	freshwater
[Ref.: #25183]	Geographic location (country and/or sea, region)	Peishih Spring in the Lugu Township of Nantou city,
[Ref.: #25183]	Country	Taiwan, Province of China
[Ref.: #25183]	Country ISO 3 Code	TWN
[Ref.: #25183]	Continent	Asia
[Ref.: #25183]	Geographic location	23.7350°/120.7810°
[Ref.: #25183]	Enrichment culture	R2A agar (BD Difco)
[Ref.: #25183]	Enrichment culture duration	3 days
[Ref.: #25183]	Enrichment culture temperature	25 ̊C
[Ref.: #25183]	Isolation/enrichment procedure	standard dilution plating method

[Ref.: #67771]	Sample type/isolated from	From freshwater of the Peishih Spring in the Lugu Township of Nantou city
[Ref.: #67771]	Country	Taiwan, Province of China
[Ref.: #67771]	Country ISO 3 Code	TWN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN890295 (>99% sequence identity) for Flectobacillus fontis from Microbeatlas

Aquatic Samples	679
Soil Samples	3
Animal Samples	93
Plant Samples	14
Total Samples	789

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25183]	Flectobacillus sp. MIB-4 partial 16S rRNA gene, strain MIB-4	LN890295	1403	GenBank ENA	1737445 tax ID

[Ref.: #25183]	GC-content	37.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	37.6 mol%

Availability in culture collections External links

[Ref.: #25183]

Culture collection no.

BCRC 80926, LMG 29292, KCTC 33763

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flectobacillus pallidus sp. nov., isolated from a freshwater spring.	Sheu SY, Liu LP, Chen WM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001774	2017	*
Phylogeny	Flectobacillus fontis sp. nov., isolated from a freshwater spring.	Chen WM, Lin KR, Young CC, Sheu SY	Int J Syst Evol Microbiol	10.1099/ijsem.0.001627	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25183

W. M. L. Chen, K. R.,Young, C. C.,Sheu, S. Y.: Flectobacillus fontis sp. nov., isolated from a freshwater spring. IJSEM 67: 336 - 342 2017 ( DOI 10.1099/ijsem.0.001627 , PubMed 27902252 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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