Streptomyces lacrimifluminis Z1027 | Type strain | CGMCC 4.7272, JCM 31054, DSM 111125

Caption:

Medium 84 28°C

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Information on culture and growth conditions Culture and growth conditions

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

ISP 6 MEDIUM (DSMZ Medium 1269)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

ISP 7 MEDIUM (DSMZ Medium 1619)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)

Culture medium growth

Culture medium link

Culture medium composition

What are isolation sources categories?

		Temperature
[Ref.: #25115]	growth	20-40 °C
[Ref.: #25115]	optimum	30 °C
[Ref.: #66470]	growth	28 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #25115]			optimum	8
[Ref.: #25115]			growth	6.0-11.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25115]	Oxygen tolerance	aerobe
[Ref.: #125438]	Oxygen tolerance	aerobe Confidence in %90.987
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %98.8

[Ref.: #25115]	Spore description	straight chains of cylindrical and smooth-surfaced white–grey spores
[Ref.: #25115]	Ability of spore formation	yes
[Ref.: #125438]	Ability of spore formation	yes Confidence in %92.078

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25115]		NaCl		maximum	4	%(w/v)
[Ref.: #25115]		NaCl		optimum	0-2.0	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-9(H₆), MK-9(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25115]	cellulose ChEBI	+	degradation
[Ref.: #25115]	D-fructose ChEBI	+	carbon source
[Ref.: #25115]	D-galactose ChEBI	+	carbon source
[Ref.: #25115]	D-glucose ChEBI	+	carbon source
[Ref.: #25115]	D-mannitol ChEBI	+	carbon source
[Ref.: #25115]	D-xylose ChEBI	+	carbon source
[Ref.: #25115]	gelatin ChEBI	+	degradation
[Ref.: #25115]	L-alanine ChEBI	-	nitrogen source
[Ref.: #25115]	L-arabinose ChEBI	+	carbon source
[Ref.: #25115]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #25115]	L-cysteine ChEBI	-	nitrogen source
[Ref.: #25115]	L-histidine ChEBI	-	nitrogen source
[Ref.: #25115]	L-rhamnose ChEBI	+	carbon source
[Ref.: #25115]	lactose ChEBI	+	carbon source
[Ref.: #25115]	leucine ChEBI	+	nitrogen source
[Ref.: #25115]	myo-inositol ChEBI	+	carbon source
[Ref.: #25115]	raffinose ChEBI	+	carbon source
[Ref.: #25115]	starch ChEBI	+	degradation
[Ref.: #25115]	sucrose ChEBI	+	carbon source
[Ref.: #25115]	tween 20 ChEBI	+	degradation
[Ref.: #25115]	tween 80 ChEBI	-	degradation
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	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #25115]		urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25115]	Sample type/isolated from	soil sample
[Ref.: #25115]	Geographic location (country and/or sea, region)	Tuotuo River, Qinghai–Tibet Plateau
[Ref.: #25115]	Country	China
[Ref.: #25115]	Country ISO 3 Code	CHN
[Ref.: #25115]	Continent	Asia
[Ref.: #25115]	Enrichment culture	Gause’s synthetic agar medium
[Ref.: #25115]	Enrichment culture composition	per litre distilled water: 20.0g soluble starch, 1.0g KNO3, 0.5g K2HPO4.3H2O, 0.5g MgSO4.7H2O, 0.001g FeSO4, 0.5g NaCl, 20.0 agar; pH 7.2
[Ref.: #25115]	Enrichment culture duration	7 days
[Ref.: #25115]	Enrichment culture temperature	30 ̊C

[Ref.: #66470]	Sample type/isolated from	Soil
[Ref.: #66470]	Geographic location (country and/or sea, region)	near the Tuotuo River Qinghai, China
[Ref.: #66470]	Country	China
[Ref.: #66470]	Country ISO 3 Code	CHN
[Ref.: #66470]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil near the Tuotuo River
[Ref.: #67770]	Geographic location (country and/or sea, region)	Qinghai
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25115]	Streptomyces lacrimifluminis strain Z1027 16S ribosomal RNA gene, partial sequence	KJ829342	1495	GenBank ENA	1500077 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptomyces lacrimifluminis CGMCC 4.7272	GCA_014646095	scaffold	GenBank ENA	1500077 tax ID	*
[Ref.: #66792]	Streptomyces lacrimifluminis strain CGMCC 4.7272	1500077.3	wgs	PATRIC	1500077 tax ID	*

[Ref.: #25115]	GC-content	73.0-75.0 mol%	Thermal denaturation, fluorometry
[Ref.: #66470]	GC-content	74.1 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	74 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces lacrimifluminis CGMCC 4.7272 NCBI: GCA_014646095.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	yes	90.294	yes
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	96.874	yes
[Ref.: #125438]	aerobic	aerobicⓘ	yes	90.987	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	yes	92.078	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	93.5	no
[Ref.: #125438]	motile2+	flagellatedⓘ	no	88.5	yes

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Streptomyces lacrimifluminis CGMCC 4.7272 NCBI: GCA_014646095
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	yes	83.2
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	90.5
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	positive	99.4
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	98.8

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