Solibacillus kalamii (DSM 101595, NRRL B-65388, ISSFR-015)

Name: Solibacillus kalamii (DSM 101595, NRRL B-65388, ISSFR-015)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 133088

Type strain

Strain Designation ISSFR-015

Culture col. no. DSM 101595 NRRL B-65388 ISSFR-015

NCBI tax ID(s) 1748298

Special Collections DSMZ Literature strains

History <- K. Venkateswaran, Jet Propulsion Laboratory, NASA; ISSFR-015

Solibacillus kalamii ISSFR-015 is an obligate aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from high-efficiency particulate arrestance filter system.

spore-forming Gram-positive motile rod-shaped colony-forming obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

Culture Collection Numbers

DSM 101595 NRRL B-65388 ISSFR-015

@ref 20215

Last LPSN update 2026-02-09 11:31:52

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Caryophanaceae

Genus Solibacillus

Species Solibacillus kalamii

Full scientific name Solibacillus kalamii Checinska Sielaff et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Confidence
25247	positive	1.1-3.6 µm	0.4-0.7 µm	rod-shaped
125439						90.7

Colony morphology

@ref	Colony color	Colony shape	Incubation period	Medium used
25247	beige	circular	2 days	TSA

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
25247	Trypticase Soy Agar (TSA)
24876	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
24876	positive	growth	30
25247	positive	growth	12-37
25247	positive	optimum	30
25247	negative	growth	42

Culture pH

@ref	Ability	Type	PH	PH range
25247	positive	optimum	8
25247	positive	growth	6.0-10.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
25247	obligate aerobe
125439	obligate aerobe	97.8

Spore formation

@ref	Spore description	Type of spore	Confidence
25247	round endospores in subterminal sporangia	endospore
125439			94.7
125438			90.486

Halophily

@ref	Salt	Growth	Tested relation	Concentration
25247	NaCl	positive	growth	0-5 %(w/v)

Observation

25247

Observationgrowth at 1 % sodium lactate

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
25247	17019 ChEBI	(R)-amygdalin	-	assimilation
25247	16651 ChEBI	(S)-lactate	-	respiration
25247	16651 ChEBI	(S)-lactate	+	builds acid from
25247	30916 ChEBI	2-oxoglutarate	-	oxidation
25247	37054 ChEBI	3-hydroxybutyrate	+	respiration
25247	37054 ChEBI	3-hydroxybutyrate	+	oxidation
25247	73918 ChEBI	3-O-methyl-D-glucose	-	oxidation
25247	18101 ChEBI	4-hydroxyphenylacetic acid	-	oxidation
25247	13705 ChEBI	acetoacetate	+	respiration
25247	22599 ChEBI	arabinose	-	assimilation
25247	73706 ChEBI	bromosuccinate	-	respiration
25247		casein	-	hydrolysis
25247	17057 ChEBI	cellobiose	-	oxidation
25247	16947 ChEBI	citrate	-	assimilation
25247	23456 ChEBI	cyclodextrin	-	assimilation
25247	18333 ChEBI	D-arabitol	-	oxidation
25247	29990 ChEBI	D-aspartate	-	oxidation
25247	15824 ChEBI	D-fructose	-	oxidation
25247	28847 ChEBI	D-fucose	-	oxidation
25247	12936 ChEBI	D-galactose	-	assimilation
25247	18024 ChEBI	D-galacturonic acid	+	respiration
25247	30612 ChEBI	D-glucarate	-	oxidation
25247	15748 ChEBI	D-glucuronate	-	respiration
25247	15588 ChEBI	D-malate	-	respiration
25247	16899 ChEBI	D-mannitol	-	assimilation
25247	16024 ChEBI	D-mannose	-	assimilation
25247	16988 ChEBI	D-ribose	-	assimilation
25247	16523 ChEBI	D-serine	-	respiration
25247	17924 ChEBI	D-sorbitol	-	assimilation
25247	65327 ChEBI	D-xylose	-	assimilation
25247	23652 ChEBI	dextrin	-	respiration
25247	15740 ChEBI	formate	-	oxidation
25247	28757 ChEBI	fructose	-	builds acid from
25247	71321 ChEBI	fusidate	-	oxidation
25247	16537 ChEBI	galactarate	-	oxidation
25247	16813 ChEBI	galactitol	-	assimilation
25247	16865 ChEBI	gamma-aminobutyric acid	+	respiration
25247	5291 ChEBI	gelatin	+	hydrolysis
25247	5291 ChEBI	gelatin	+	respiration
25247	28066 ChEBI	gentiobiose	-	oxidation
25247	17234 ChEBI	glucose	-	fermentation
25247	32323 ChEBI	glucuronamide	+	oxidation
25247	17754 ChEBI	glycerol	-	respiration
25247	70744 ChEBI	glycine-proline	+	respiration
25247	32735 ChEBI	guanidinium chloride	+	respiration
25247	17596 ChEBI	inosine	-	oxidation
25247	16977 ChEBI	L-alanine	-	respiration
25247	16467 ChEBI	L-arginine	+	respiration
25247	29991 ChEBI	L-aspartate	+	respiration
25247	29985 ChEBI	L-glutamate	-	respiration
25247	15971 ChEBI	L-histidine	-	respiration
25247	15589 ChEBI	L-malate	+	respiration
25247	18183 ChEBI	L-pyroglutamic acid	+	oxidation
25247	62345 ChEBI	L-rhamnose	-	oxidation
25247	17115 ChEBI	L-serine	-	respiration
25247	17716 ChEBI	lactose	-	assimilation
25247	17306 ChEBI	maltose	-	assimilation
25247	29864 ChEBI	mannitol	-	assimilation
25247	37684 ChEBI	mannose	-	assimilation
25247	28053 ChEBI	melibiose	-	oxidation
25247	74611 ChEBI	methyl (R)-lactate	-	oxidation
25247	320055 ChEBI	methyl beta-D-glucopyranoside	-	assimilation
25247	37657 ChEBI	methyl D-glucoside	-	oxidation
25247	17268 ChEBI	myo-inositol	-	oxidation
25247	63153 ChEBI	N-acetyl-D-mannosamine	-	oxidation
25247	28037 ChEBI	N-acetylgalactosamine	-	oxidation
25247	506227 ChEBI	N-acetylglucosamine	-	assimilation
25247	17632 ChEBI	nitrate	-	reduction
25247	17309 ChEBI	pectin	-	oxidation
25247	17272 ChEBI	propionate	-	oxidation
25247	27941 ChEBI	pullulan	-	assimilation
25247	26490 ChEBI	quinate	+	respiration
25247	16634 ChEBI	raffinose	-	assimilation
25247	16634 ChEBI	raffinose	-	oxidation
25247	15963 ChEBI	ribitol	-	assimilation
25247	17814 ChEBI	salicin	-	oxidation
25247	17164 ChEBI	stachyose	-	respiration
25247	28017 ChEBI	starch	-	hydrolysis
25247	17992 ChEBI	sucrose	-	oxidation
25247	75198 ChEBI	tetrazolium blue	-	respiration
25247	75193 ChEBI	tetrazolium violet	-	respiration
25247	27082 ChEBI	trehalose	-	builds acid from
25247	32528 ChEBI	turanose	-	oxidation
25247	53423 ChEBI	tween 40	-	respiration
25247	53426 ChEBI	tween 80	+	hydrolysis
25247	18222 ChEBI	xylose	-	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite
25247	15688 ChEBI	acetoin
25247	16136 ChEBI	hydrogen sulfide
25247	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
25247	15688 ChEBI	acetoin	-
25247	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
25247	alpha-galactosidase	-	3.2.1.22
25247	alpha-glucosidase	-	3.2.1.20
25247	alpha-mannosidase	-	3.2.1.24
25247	arginine dihydrolase	+	3.5.3.6
25247	beta-galactopyranosidase	-
25247	beta-galactosidase	-	3.2.1.23
25247	beta-glucosidase	-	3.2.1.21
25247	beta-glucuronidase	-	3.2.1.31
25247	catalase	+	1.11.1.6
25247	cytochrome oxidase	+	1.9.3.1
25247	leucine arylamidase	-	3.4.11.1
25247	phenylalanine arylamidase	-
25247	phosphatase	-
25247	phosphatidylinositol phospholipase C	-	3.1.4.11
25247	proline-arylamidase	-	3.4.11.5
25247	pyrrolidonyl arylamidase	+	3.4.19.3
25247	tripeptide aminopeptidase	-	3.4.11.4
25247	tyrosine arylamidase	+
25247	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

@ref

25247

Fatty acid	Percentage
C15:0 iso	45.2
C17:1ω10c	12.1
alcohol C16:1ω7c	9.1
C16:0 iso	8.1
C17:0 iso	7.9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Engineered product

Isolation

@ref	Sample type	Geographic location
24876	high-efficiency particulate arrestance (HEPA) filter system	International Space Station (ISS), node 2

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24876	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM217453v1 assembly for Solibacillus kalamii ISSFR-015	contig	GCA_002174535	1748298.3	2791354932	1748298	69.87
66792	ASM1690902v1 assembly for Solibacillus kalamii DSM 101595	contig	GCA_016909025	1748298.10	2901246952	1748298	69.71

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24876	Solibacillus kalamii strain ISSFR-015 16S ribosomal RNA gene, partial sequence	KT763359	1494		1748298

GC content

@ref	GC-content (mol%)	Method
24876	45.4	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Solibacillus kalamii ISSFR-015
NCBI: GCA_002174535

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	94.70	no
125439	motility	BacteriaNetⓘ	yes	90.70	no
125439	gram_stain	BacteriaNetⓘ	variable	80.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Solibacillus kalamii strain DSM 101595
PATRIC: 1748298.10

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	75.42	yes
125438	anaerobic	anaerobicⓘ	no	94.72	yes
125438	spore-forming	spore-formingⓘ	yes	90.49	yes
125438	aerobic	aerobicⓘ	yes	84.05	yes
125438	thermophilic	thermophileⓘ	no	93.76	yes
125438	flagellated	motile2+ⓘ	yes	84.03	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Solibacillus kalamii, Isolated from an Air Filter Aboard the International Space Station.	Seuylemezian A, Singh NK, Vaishampayan P, Venkateswaran K	Genome Announc	10.1128/genomeA.00696-17	2017
Phylogeny	Solibacillus kalamii sp. nov., isolated from a high-efficiency particulate arrestance filter system used in the International Space Station.	Checinska Sielaff A, Kumar RM, Pal D, Mayilraj S, Venkateswaran K	Int J Syst Evol Microbiol	10.1099/ijsem.0.001706	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24876	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101595
#25247	A. K. Checinska Sielaff, R. M.,Pal, D.,Mayilraj, S.,Venkateswaran, K.: Solibacillus kalamii sp. nov., isolated from a high-efficiency particulate arrestance filter system used in the International Space Station. IJSEM 67: 896 - 901 2017 ( DOI 10.1099/ijsem.0.001706 , PubMed 28475026 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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Solibacillus kalamii (DSM 101595, NRRL B-65388, ISSFR-015)

Name and taxonomic classification

Culture Collection Numbers

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002174535

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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