Name: Caenimicrobium hargitense CGII-59m2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133087

Type strain:

Species: Caenimicrobium hargitense

Strain Designation: CGII-59m2

Culture col. no.: DSM 29806, NCAIM B.02615

Strain history: <- T. Felföldi, Eötvös Loránd Univ. Budapest, Hungary <- I.Máthé, R.-D. Fikó and E. Kovács,Sapientia University of Transylvania, Miercurea Ciuc, Romania

NCBI tax ID(s): 1549637 (species)

SI-ID 406462

DSM 29806

Section

Caenimicrobium hargitense CGII-59m2 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from activated sludge from a bioreactor treating landfill leachate.

colony-forming
Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Caenimicrobium*
	Species	**Caenimicrobium hargitense**
	Full Scientific Name (LPSN)	Caenimicrobium hargitense Felföldi et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25218]	Gram stain	negative

[Ref.: #25218]	Cell length	1.1-1.2 µm

[Ref.: #25218]	Cell width	0.6-0.8 µm

[Ref.: #25218]	Cell shape	rod-shaped

[Ref.: #25218]	Motility	no

[Ref.: #25218]	Colony size	2-3 mm
[Ref.: #25218]	Colony color	beige
[Ref.: #25218]	Colony shape	circular
[Ref.: #25218]	Cultivation medium used	NA

Multimedia content

[Ref.: #24875]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25218]

Culture medium

Nutrient agar (NA)

[Ref.: #25218]

Culture medium growth

[Ref.: #24875]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #24875]

Culture medium growth

[Ref.: #24875]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24875]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24875]			growth	28 °C
[Ref.: #25218]			growth	4-65 °C

[Ref.: #24875]	Temperature range	mesophilic
[Ref.: #25218]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25218]			growth	7-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25218]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25218]		NaCl		growth	0-8	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25218]	2-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #25218]	5-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #25218]	amygdalin ChEBI	-	assimilation
[Ref.: #25218]	arbutin ChEBI	-	assimilation
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #25218]	cellobiose ChEBI	-	assimilation
[Ref.: #25218]	D-arabinose ChEBI	-	assimilation
[Ref.: #25218]	D-arabitol ChEBI	-	assimilation
[Ref.: #25218]	D-fructose ChEBI	-	assimilation
[Ref.: #25218]	D-fucose ChEBI	-	assimilation
[Ref.: #25218]	D-galactose ChEBI	-	assimilation
[Ref.: #25218]	D-glucose ChEBI	-	builds acid from
[Ref.: #25218]	D-glucose ChEBI	-	assimilation
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #25218]	D-lyxose ChEBI	-	assimilation
[Ref.: #25218]	D-mannitol ChEBI	-	assimilation
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #25218]	D-mannose ChEBI	-	assimilation
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25218]	D-ribose ChEBI	-	assimilation
[Ref.: #25218]	D-sorbitol ChEBI	-	assimilation
[Ref.: #25218]	D-tagatose ChEBI	-	assimilation
[Ref.: #25218]	D-xylose ChEBI	-	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25218]	erythritol ChEBI	-	assimilation
[Ref.: #25218]	esculin ChEBI	-	assimilation
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #25218]	galactitol ChEBI	-	assimilation
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #25218]	gentiobiose ChEBI	-	assimilation
[Ref.: #25218]	gluconate ChEBI	-	assimilation
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25218]	glycerol ChEBI	-	assimilation
[Ref.: #25218]	glycogen ChEBI	-	assimilation
[Ref.: #25218]	inulin ChEBI	-	assimilation
[Ref.: #25218]	L-arabinose ChEBI	-	assimilation
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25218]	L-arabitol ChEBI	-	assimilation
[Ref.: #25218]	L-fucose ChEBI	-	assimilation
[Ref.: #25218]	L-rhamnose ChEBI	-	assimilation
[Ref.: #25218]	L-sorbose ChEBI	-	assimilation
[Ref.: #25218]	L-xylose ChEBI	-	assimilation
[Ref.: #25218]	lactose ChEBI	-	assimilation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #25218]	maltose ChEBI	-	assimilation
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25218]	melezitose ChEBI	-	assimilation
[Ref.: #25218]	melibiose ChEBI	-	assimilation
[Ref.: #25218]	methyl alpha-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #25218]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #25218]	methyl beta-D-xylopyranoside ChEBI	-	assimilation
[Ref.: #25218]	myo-inositol ChEBI	-	assimilation
[Ref.: #25218]	N-acetyl-D-glucosamine ChEBI	-	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #25218]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #25218]	raffinose ChEBI	-	assimilation
[Ref.: #25218]	ribitol ChEBI	-	assimilation
[Ref.: #25218]	salicin ChEBI	-	assimilation
[Ref.: #25218]	starch ChEBI	-	hydrolysis
[Ref.: #25218]	starch ChEBI	-	assimilation
[Ref.: #25218]	sucrose ChEBI	-	assimilation
[Ref.: #25218]	trehalose ChEBI	-	assimilation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #25218]	turanose ChEBI	-	assimilation
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #25218]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #25218]	acid phosphatase	-	3.1.3.2
[Ref.: #25218]	alkaline phosphatase	+/-	3.1.3.1
[Ref.: #25218]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25218]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25218]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25218]	alpha-glucosidase	-	3.2.1.20
[Ref.: #25218]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #25218]	beta-galactosidase	-	3.2.1.23
[Ref.: #25218]	beta-glucosidase	-	3.2.1.21
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #25218]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25218]	catalase	+	1.11.1.6
[Ref.: #25218]	cystine arylamidase	-	3.4.11.3
[Ref.: #25218]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #25218]	esterase (C 4)	+
[Ref.: #25218]	esterase Lipase (C 8)	+/-
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #25218]	leucine arylamidase	+	3.4.11.1
[Ref.: #25218]	lipase (C 14)	-
[Ref.: #25218]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25218]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25218]	phosphatase	-
[Ref.: #25218]	trypsin	-	3.4.21.4
[Ref.: #25218]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #25218]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25218]

Fatty acid	Percentage
C16:1ω7c	28.7
C16:0	28.5
cyclo C17:0	13.7
C18:1ω7c	13.6
C14:0 3OH	7
C12:0	5.4

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #24875]	23297	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	+	-	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24875]	Sample type/isolated from	activated sludge from a bioreactor treating landfill leachate
[Ref.: #24875]	Geographic location (country and/or sea, region)	Harghita County, Odorheiu Secuiesc
[Ref.: #24875]	Country	Romania
[Ref.: #24875]	Country ISO 3 Code	ROU
[Ref.: #24875]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Bioreactor	-
#Engineered	#Waste	#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KM083134 (>99% sequence identity) for Caenimicrobium from Microbeatlas

Aquatic Samples	93
Soil Samples	58
Animal Samples	195
Plant Samples	8
Total Samples	354

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24875]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24875]	Caenimicrobium hargitense strain CGII-59m2 16S ribosomal RNA gene, partial sequence	KM083134	1453	ENA	1549637 tax ID

[Ref.: #24875]

GC-content

62.3 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24875]

Culture collection no.

DSM 29806, NCAIM B.02615

[Ref.: #91352]

SI-ID 406462

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge.	Felfoldi T, Schumann P, Mentes A, Keki Z, Mathe I, Toth EM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001674	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24875

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29806

#25218

T. S. Felföldi, P.,Mentes, A.,Kéki, Z.,Máthé, I.,Tóth, E. M.: Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge. IJSEM 67: 627 - 632 2017 ( DOI 10.1099/ijsem.0.001674 , PubMed 27902326 )

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#91352

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406462.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Caenimicrobium hargitense in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF