Name: Bacillus wiedmannii DSM 102050
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133012

Type strain:

Species: Bacillus wiedmannii

Culture col. no.: DSM 102050, CIP 111364, LMG 29269, FSL W8-0169

Strain history: <- K. Boor, Cornell Univerity <- Matthew Watterson, Cornell University, Ithaca, USA; FSL W8-0169

NCBI tax ID(s): 1890302 (species)

Section

Bacillus wiedmannii DSM 102050 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from raw milk stored in a dairy powder processing plant.

colony-forming
Gram-positive
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	25-08-2022 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Bacillota
	Class	Bacilli
	Order	Caryophanales
	Family	Bacillaceae
	Genus	Bacillus
	Species	Bacillus wiedmannii
	Full Scientific Name (PNU)	Bacillus wiedmannii Miller et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #25027]	Gram stain	positive
[Ref.: #25027]	Cell length	2.8 µm
[Ref.: #25027]	Cell width	1.2 µm
[Ref.: #25027]	Cell shape	rod-shaped

[Ref.: #25027]	Colony color	creamy
[Ref.: #25027]	Colony shape	circular
[Ref.: #25027]	Incubation period	1 day
[Ref.: #25027]	Cultivation medium used	BHI agar (brain heart infusion medium, Becton Dickinson)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25027]

Culture medium

BHI agar

[Ref.: #25027]

Culture medium growth

[Ref.: #24800]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #24800]

Culture medium growth

[Ref.: #24800]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24800]

Culture medium composition

expand / minimize

[Ref.: #24800]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24800]

Culture medium growth

[Ref.: #24800]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24800]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24800]	growth	30 °C
[Ref.: #25027]	growth	5-43 °C
[Ref.: #25027]	optimum	20-40 °C

[Ref.: #24800]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

facultative anaerobe

[Ref.: #25027]	Spore description	ellipsoidal, present in the centre of vegetative cells, non-swollen sporangia
[Ref.: #25027]	Type of spore	endospore
[Ref.: #25027]	Ability of spore formation	yes

[Ref.: #24800]

Name of produced compound

haemolysin

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25027]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #25027]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #25027]	arbutin ChEBI	+	builds acid from
[Ref.: #25027]	casein	+	hydrolysis
[Ref.: #25027]	cellobiose ChEBI	+	builds acid from
[Ref.: #25027]	D-arabinose ChEBI	-	builds acid from
[Ref.: #25027]	D-arabitol ChEBI	-	builds acid from
[Ref.: #25027]	D-fructose ChEBI	+	builds acid from
[Ref.: #25027]	D-fucose ChEBI	-	builds acid from
[Ref.: #25027]	D-glucose ChEBI	+	builds acid from
[Ref.: #25027]	D-lyxose ChEBI	-	builds acid from
[Ref.: #25027]	D-mannitol ChEBI	-	builds acid from
[Ref.: #25027]	D-mannose ChEBI	-	builds acid from
[Ref.: #25027]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25027]	D-tagatose ChEBI	-	builds acid from
[Ref.: #25027]	D-xylose ChEBI	-	builds acid from
[Ref.: #25027]	erythritol ChEBI	-	builds acid from
[Ref.: #25027]	esculin ChEBI	+	builds acid from
[Ref.: #25027]	galactitol ChEBI	-	builds acid from
[Ref.: #25027]	galactose ChEBI	-	builds acid from
[Ref.: #25027]	gelatin ChEBI	+	builds acid from
[Ref.: #25027]	gentiobiose ChEBI	-	builds acid from
[Ref.: #25027]	glycerol ChEBI	-	builds acid from
[Ref.: #25027]	glycogen ChEBI	+	builds acid from
[Ref.: #25027]	inulin ChEBI	-	builds acid from
[Ref.: #25027]	L-arabinose ChEBI	-	builds acid from
[Ref.: #25027]	L-arabitol ChEBI	-	builds acid from
[Ref.: #25027]	L-fucose ChEBI	-	builds acid from
[Ref.: #25027]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #25027]	L-sorbose ChEBI	-	builds acid from
[Ref.: #25027]	L-xylose ChEBI	-	builds acid from
[Ref.: #25027]	maltose ChEBI	+	builds acid from
[Ref.: #25027]	melezitose ChEBI	-	builds acid from
[Ref.: #25027]	melibiose ChEBI	-	builds acid from
[Ref.: #25027]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #25027]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #25027]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #25027]	myo-inositol ChEBI	-	builds acid from
[Ref.: #25027]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #25027]	raffinose ChEBI	-	builds acid from
[Ref.: #25027]	ribitol ChEBI	-	builds acid from
[Ref.: #25027]	ribose ChEBI	+	builds acid from
[Ref.: #25027]	salicin ChEBI	+	builds acid from
[Ref.: #25027]	starch ChEBI	+	hydrolysis
[Ref.: #25027]	sucrose ChEBI	-	builds acid from
[Ref.: #25027]	trehalose ChEBI	+	builds acid from
[Ref.: #25027]	tryptophan ChEBI	-	energy source
[Ref.: #25027]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #25027]		acetoin ChEBI
[Ref.: #25027]		indole ChEBI

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #25027]		acetoin ChEBI	+
[Ref.: #25027]		indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #25027]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25027]	lecithinase	+
[Ref.: #25027]	lysine decarboxylase	-	4.1.1.18
[Ref.: #25027]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #25027]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25027]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24800]	Sample type/isolated from	raw milk stored in a dairy powder processing plant
[Ref.: #24800]	Country	USA
[Ref.: #24800]	Country ISO 3 Code	USA
[Ref.: #24800]	Continent	North America

[Ref.: #25027]	Sample type/isolated from	silo raw milk sample collected from a dairy powder processing plant
[Ref.: #25027]	Geographic location (country and/or sea, region)	north-eastern USA
[Ref.: #25027]	Isolation date	2012

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Engineered	#Food production	#Dairy product
#Engineered	#Industrial	#Plant (Factory)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25027]	16S rRNA	SRX1297474		GenBank
[Ref.: #24800]	Bacillus wiedmannii strain FSL W8-0169 16S ribosomal RNA gene, partial sequence	KU198626	1540	ENA	1890302 tax ID

[Ref.: #25027]	GC-content	35.3 mol%	genome sequence analysis
[Ref.: #24800]	GC-content	35.3 mol%	sequence analysis

Availability in culture collections External links

[Ref.: #24800]

Culture collection no.

DSM 102050, CIP 111364, LMG 29269, FSL W8-0169

Literature:

Title	Authors	Journal	DOI	Year	Mesh	Topic	Topic2
Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments.	Miller RA, Beno SM, Kent DJ, Carroll LM, Martin NH, Boor KJ, Kovac J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001421	2016	Bacillus/classification/genetics/isolation & purification, Bacillus cereus/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Dairy Products/microbiology, Dairying, Fatty Acids/chemistry, HeLa Cells, Humans, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, United States	Phylogeny	Pathogenicity	*
Characterization of the Genome Feature and Toxic Capacity of a Bacillus wiedmannii Isolate From the Hydrothermal Field in Okinawa Trough.	Zhao Y, Chen C, Gu HJ, Zhang J, Sun L	Front Cell Infect Microbiol	10.3389/fcimb.2019.00370	2019	Animals, Bacillus/genetics/isolation & purification, Environmental Microbiology, Female, Fishes/microbiology, Genome, Bacterial, Genomics/methods, Hydrothermal Vents/microbiology, Japan, Mice, Phylogeny, RNA, Ribosomal, 16S/genetics, Virulence/genetics, Whole Genome Sequencing	Phylogeny	Genetics	*
Bacillus fungorum sp. nov., a bacterium isolated from spent mushroom substrate.	Liu X, Wang L, Han M, Xue QH, Zhang GS, Gao J, Sun X	Int J Syst Evol Microbiol	10.1099/ijsem.0.003673	2020	Agaricales, Bacillus/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry	Phylogeny	Transcriptome	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24800

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102050

#25027

R. A. B. Miller, S. M.,Kent, D. J.,Carroll, L. M.,Martin, N. H.,Boor, K. J.,Kovac, J.: Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments. IJSEM 66: 4744 - 4753 2016 ( DOI 10.1099/ijsem.0.001421 , PubMed 27520992 )

* These data were automatically processed and therefore are not curated

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