Bacillus wiedmannii FSL W8-0169 | Type strain | DSM 102050, CIP 111364, LMG 29269

Strain identifier

BacDive ID: 133012

Type strain:

Species: Bacillus wiedmannii

Strain Designation: FSL W8-0169

Culture col. no.: DSM 102050, CIP 111364, LMG 29269

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #24800]	Domain	Bacteria
[Ref.: #24800]	Phylum	Firmicutes
[Ref.: #24800]	Class	Bacilli
[Ref.: #24800]	Order	Bacillales
[Ref.: #24800]	Family	Bacillaceae
[Ref.: #24800]	Genus	Bacillus
[Ref.: #24800]	Species	Bacillus wiedmannii
[Ref.: #24800]	Full Scientific Name	Bacillus wiedmannii Miller et al. 2016
[Ref.: #24800]	Strain Designation	FSL W8-0169
[Ref.: #24800]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	20-11-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Firmicutes
[Ref.: #20215]	Class	Bacilli		Int. J. Syst. Bacteriol. 38:220
[Ref.: #20215]	Family	Bacillaceae		Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Genus	Bacillus	genus (AL)	Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Species	Bacillus wiedmannii	sp. nov. (VP)	Int. J. Syst. Evol. Microbiol. 66:4744*
[Ref.: #20215]	Full Scientific Name	Bacillus wiedmannii Miller et al. 2016

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #25027]	Gram stain	positive
[Ref.: #25027]	Cell length	2.8 µm
[Ref.: #25027]	Cell width	1.2 µm
[Ref.: #25027]	Cell shape	rod-shaped

[Ref.: #25027]	Colony color	creamy
[Ref.: #25027]	Colony shape	circular
[Ref.: #25027]	Incubation period	1 day
[Ref.: #25027]	Cultivation medium used	BHI agar (brain heart infusion medium, Becton Dickinson)

[Ref.: #24800]

Name of produced compound

haemolysin

	Enzyme	Enzyme activity	EC number
[Ref.: #25027]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25027]	lecithinase	+
[Ref.: #25027]	lysine decarboxylase	-	4.1.1.18
[Ref.: #25027]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #25027]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25027]	urease	-	3.5.1.5

	Metabolite production	Metabolite	Production
[Ref.: #25027]		Acetoin	yes
[Ref.: #25027]		Indole	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #25027]		Acetoin	+
[Ref.: #25027]		Indole		-

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25027]	2-Dehydro-D-gluconate	-	builds acid from
[Ref.: #25027]	5-Dehydro-D-gluconate	-	builds acid from
[Ref.: #25027]	Arbutin	+	builds acid from
[Ref.: #25027]	Casein	+	hydrolysis
[Ref.: #25027]	Cellobiose	+	builds acid from
[Ref.: #25027]	D-Adonitol	-	builds acid from
[Ref.: #25027]	D-Arabinose	-	builds acid from
[Ref.: #25027]	D-Arabitol	-	builds acid from
[Ref.: #25027]	D-Fructose	+	builds acid from
[Ref.: #25027]	D-Fucose	-	builds acid from
[Ref.: #25027]	D-Glucose	+	builds acid from
[Ref.: #25027]	D-Lyxose	-	builds acid from
[Ref.: #25027]	D-Mannitol	-	builds acid from
[Ref.: #25027]	D-Mannose	-	builds acid from
[Ref.: #25027]	D-Sorbitol	-	builds acid from
[Ref.: #25027]	D-Tagatose	-	builds acid from
[Ref.: #25027]	D-Xylose	-	builds acid from
[Ref.: #25027]	Erythritol	-	builds acid from
[Ref.: #25027]	Esculin hydrolysate	+	builds acid from
[Ref.: #25027]	Galactitol	-	builds acid from
[Ref.: #25027]	Galactose	-	builds acid from
[Ref.: #25027]	Gelatin	+	builds acid from
[Ref.: #25027]	Gentiobiose	-	builds acid from
[Ref.: #25027]	Glycerol	-	builds acid from
[Ref.: #25027]	Glycogen	+	builds acid from
[Ref.: #25027]	Inulin	-	builds acid from
[Ref.: #25027]	L-Arabinose	-	builds acid from
[Ref.: #25027]	L-Arabitol	-	builds acid from
[Ref.: #25027]	L-Fucose	-	builds acid from
[Ref.: #25027]	L-Rhamnose	-	builds acid from
[Ref.: #25027]	L-Sorbose	-	builds acid from
[Ref.: #25027]	L-Xylose	-	builds acid from
[Ref.: #25027]	Maltose	+	builds acid from
[Ref.: #25027]	Melezitose	-	builds acid from
[Ref.: #25027]	Melibiose	-	builds acid from
[Ref.: #25027]	Methyl alpha-D-glucopyranoside	-	builds acid from
[Ref.: #25027]	Methyl alpha-D-mannoside	-	builds acid from
[Ref.: #25027]	Methyl beta-D-xylopyranoside	-	builds acid from
[Ref.: #25027]	myo-Inositol	-	builds acid from
[Ref.: #25027]	N-Acetylglucosamine	+	builds acid from
[Ref.: #25027]	Raffinose	-	builds acid from
[Ref.: #25027]	Ribose	+	builds acid from
[Ref.: #25027]	Salicin	+	builds acid from
[Ref.: #25027]	Starch	+	hydrolysis
[Ref.: #25027]	Sucrose	-	builds acid from
[Ref.: #25027]	Trehalose	+	builds acid from
[Ref.: #25027]	Tryptophan	-	energy source
[Ref.: #25027]	Xylitol	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #25027]

Oxygen tolerance

facultative anaerobe

[Ref.: #25027]	Spore description	ellipsoidal, present in the centre of vegetative cells, non-swollen sporangia
[Ref.: #25027]	Type of spore	endospore
[Ref.: #25027]	Ability of spore formation	yes

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #25027]

Culture medium

BHI agar

[Ref.: #25027]

Culture medium growth

[Ref.: #24800]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1), 30°C

[Ref.: #24800]

Culture medium growth

[Ref.: #24800]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #24800]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693), 30°C

[Ref.: #24800]

Culture medium growth

[Ref.: #24800]

Culture medium link

medium recipe provided by DSMZ

		Temperature
[Ref.: #24800]	growth	30 ̊C
[Ref.: #25027]	growth	5-43 ̊C
[Ref.: #25027]	optimum	20-40 ̊C

[Ref.: #24800]

Temperature range

mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #24800]

Sample type/isolated from

raw milk stored in a dairy powder processing plant

[Ref.: #24800]

Country

USA

[Ref.: #24800]

Continent

North America

[Ref.: #25027]

Sample type/isolated from

silo raw milk sample
collected from a dairy powder processing plant

[Ref.: #25027]

Geographic location (country and/or sea, region)

north-eastern USA

[Ref.: #25027]

Isolation date

2012

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Engineered	#Food production	#Dairy product
#Engineered	#Industrial	#Plant (Factory)

What are isolation sources categories?

* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #25027]	GC-content	35.3 mol%	genome sequence analysis
[Ref.: #24800]	GC-content	35.3 mol%	sequence analysis

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #24800]	whole genome shotgun sequence, Whole Genome Shotgun (WGS) Sequence INSDC	Bacillus wiedmannii strain FSL W8-0169, whole genome shotgun sequencing project	LOBC00000000		104	1890302
[Ref.: #24800]	16S rRNA gene, Marker Gene (GenBank Direct submission)	Bacillus wiedmannii strain FSL W8-0169 16S ribosomal RNA gene, partial sequence	KU198626		1540	1890302
[Ref.: #25027]	GenBank/EMBL/DDBJ	16S rRNA	KU198626/SRX1297474	16S

Availability in culture collectionsStrain availability

[Ref.: #24800]

Culture collection no.

DSM 102050, CIP 111364, LMG 29269

[Ref.: #24800]

Strain history

<- K. Boor, Cornell Univerity <- Matthew Watterson, Cornell University, Ithaca, USA; FSL W8-0169

References

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#24800

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102050

#25027

R. A. B. Miller, S. M.,Kent, D. J.,Carroll, L. M.,Martin, N. H.,Boor, K. J.,Kovac, J.: Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments. IJSEM 66: 4744 - 4753 2016 ( DOI 10.1099/ijsem.0.001421 )

* These References are textmined

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