Bacillus wiedmannii FSL W8-0169 | Type strain | DSM 102050, CIP 111364, LMG 29269

Strain identifier

BacDive ID: 133012

Type strain:

Species: Bacillus wiedmannii

Strain Designation: FSL W8-0169

Culture col. no.: DSM 102050, CIP 111364, LMG 29269

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Molecular biology
Strain availability

External links

Bacillus wiedmannii FSL W8-0169 is a facultative anaerobe, spore-forming, gram-positive bacterium that forms circular colonies and was isolated from raw milk stored in a dairy powder processing plant.

colony-forming
gram-positive
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	10-09-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Firmicutes
	Class	Bacilli
	Order	Caryophanales
	Family	Bacillaceae
	Genus	Bacillus
	Species	Bacillus wiedmannii
	Full Scientific Name (PNU)	Bacillus wiedmannii Miller et al. 2016

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #25027]	Gram stain	positive
[Ref.: #25027]	Cell length	2.8 µm
[Ref.: #25027]	Cell width	1.2 µm
[Ref.: #25027]	Cell shape	rod-shaped

[Ref.: #25027]	Colony color	creamy
[Ref.: #25027]	Colony shape	circular
[Ref.: #25027]	Incubation period	1 day
[Ref.: #25027]	Cultivation medium used	BHI agar (brain heart infusion medium, Becton Dickinson)

[Ref.: #24800]

Name of produced compound

haemolysin

	Enzyme	Enzyme activity	EC number
[Ref.: #25027]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25027]	lecithinase	+
[Ref.: #25027]	lysine decarboxylase	-	4.1.1.18
[Ref.: #25027]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #25027]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25027]	urease	-	3.5.1.5

	Metabolite production	Metabolite	Production
[Ref.: #25027]		acetoin ChEBI	yes
[Ref.: #25027]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #25027]		acetoin ChEBI	+
[Ref.: #25027]		indole ChEBI		-

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25027]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #25027]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #25027]	arbutin ChEBI	+	builds acid from
[Ref.: #25027]	casein	+	hydrolysis
[Ref.: #25027]	cellobiose ChEBI	+	builds acid from
[Ref.: #25027]	D-arabinose ChEBI	-	builds acid from
[Ref.: #25027]	D-arabitol ChEBI	-	builds acid from
[Ref.: #25027]	D-fructose ChEBI	+	builds acid from
[Ref.: #25027]	D-fucose ChEBI	-	builds acid from
[Ref.: #25027]	D-glucose ChEBI	+	builds acid from
[Ref.: #25027]	D-lyxose ChEBI	-	builds acid from
[Ref.: #25027]	D-mannitol ChEBI	-	builds acid from
[Ref.: #25027]	D-mannose ChEBI	-	builds acid from
[Ref.: #25027]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25027]	D-tagatose ChEBI	-	builds acid from
[Ref.: #25027]	D-xylose ChEBI	-	builds acid from
[Ref.: #25027]	erythritol ChEBI	-	builds acid from
[Ref.: #25027]	esculin ChEBI	+	builds acid from
[Ref.: #25027]	galactitol ChEBI	-	builds acid from
[Ref.: #25027]	galactose ChEBI	-	builds acid from
[Ref.: #25027]	gelatin ChEBI	+	builds acid from
[Ref.: #25027]	gentiobiose ChEBI	-	builds acid from
[Ref.: #25027]	glycerol ChEBI	-	builds acid from
[Ref.: #25027]	glycogen ChEBI	+	builds acid from
[Ref.: #25027]	inulin ChEBI	-	builds acid from
[Ref.: #25027]	L-arabinose ChEBI	-	builds acid from
[Ref.: #25027]	L-arabitol ChEBI	-	builds acid from
[Ref.: #25027]	L-fucose ChEBI	-	builds acid from
[Ref.: #25027]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #25027]	L-sorbose ChEBI	-	builds acid from
[Ref.: #25027]	L-xylose ChEBI	-	builds acid from
[Ref.: #25027]	maltose ChEBI	+	builds acid from
[Ref.: #25027]	melezitose ChEBI	-	builds acid from
[Ref.: #25027]	melibiose ChEBI	-	builds acid from
[Ref.: #25027]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #25027]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #25027]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #25027]	myo-inositol ChEBI	-	builds acid from
[Ref.: #25027]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #25027]	raffinose ChEBI	-	builds acid from
[Ref.: #25027]	ribitol ChEBI	-	builds acid from
[Ref.: #25027]	ribose ChEBI	+	builds acid from
[Ref.: #25027]	salicin ChEBI	+	builds acid from
[Ref.: #25027]	starch ChEBI	+	hydrolysis
[Ref.: #25027]	sucrose ChEBI	-	builds acid from
[Ref.: #25027]	trehalose ChEBI	+	builds acid from
[Ref.: #25027]	tryptophan ChEBI	-	energy source
[Ref.: #25027]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #25027]

Oxygen tolerance

facultative anaerobe

[Ref.: #25027]	Spore description	ellipsoidal, present in the centre of vegetative cells, non-swollen sporangia
[Ref.: #25027]	Type of spore	endospore
[Ref.: #25027]	Ability of spore formation	yes

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #25027]

Culture medium

BHI agar

[Ref.: #25027]

Culture medium growth

[Ref.: #24800]

Culture medium

Nutrient agar (NA)

[Ref.: #24800]

Culture medium growth

[Ref.: #24800]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #24800]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24800]

Culture medium growth

[Ref.: #24800]

Culture medium link

medium recipe provided by DSMZ

		Temperature
[Ref.: #24800]	growth	30 ̊C
[Ref.: #25027]	growth	5-43 ̊C
[Ref.: #25027]	optimum	20-40 ̊C

[Ref.: #24800]

Temperature range

mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #24800]

Sample type/isolated from

raw milk stored in a dairy powder processing plant

[Ref.: #24800]

Country

USA

[Ref.: #24800]

Country ISO 3 Code

USA

[Ref.: #24800]

Continent

North America

[Ref.: #25027]

Sample type/isolated from

silo raw milk sample
collected from a dairy powder processing plant

[Ref.: #25027]

Geographic location (country and/or sea, region)

north-eastern USA

[Ref.: #25027]

Isolation date

2012

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Engineered	#Food production	#Dairy product
#Engineered	#Industrial	#Plant (Factory)

What are isolation sources categories?

* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #25027]	GC-content	35.3 mol%	genome sequence analysis
[Ref.: #24800]	GC-content	35.3 mol%	sequence analysis

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #24800]	whole genome shotgun sequence, Whole Genome Shotgun (WGS) Sequence INSDC	Bacillus wiedmannii strain FSL W8-0169, whole genome shotgun sequencing project	LOBC00000000 ENA		104	1890302 tax ID
[Ref.: #24800]	16S rRNA gene, Marker Gene (GenBank Direct submission)	Bacillus wiedmannii strain FSL W8-0169 16S ribosomal RNA gene, partial sequence	KU198626 ENA	16S	1540	1890302 tax ID
[Ref.: #25027]	GenBank/EMBL/DDBJ	16S rRNA	KU198626/SRX1297474 GenBank	16S

Availability in culture collectionsStrain availability

[Ref.: #24800]

Culture collection no.

DSM 102050, CIP 111364, LMG 29269

[Ref.: #24800]

Strain history

<- K. Boor, Cornell Univerity <- Matthew Watterson, Cornell University, Ithaca, USA; FSL W8-0169

References

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#24800

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102050

#25027

R. A. B. Miller, S. M.,Kent, D. J.,Carroll, L. M.,Martin, N. H.,Boor, K. J.,Kovac, J.: Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments. IJSEM 66: 4744 - 4753 2016 ( DOI 10.1099/ijsem.0.001421 )

* These References are textmined

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