Strain identifier
BacDive ID: 133012
Type strain:
Species: Bacillus wiedmannii
Strain history: CIP <- 2017, DSMZ <- K. Boor, Cornell Univ., Ithaca, NY, USA <- M. Watterson, Cornell Univ., Ithaca, NY, USA: strain FSL W8-0169
NCBI tax ID(s): 1890302 (species)
General
@ref: 24800
BacDive-ID: 133012
DSM-Number: 102050
keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, Gram-positive, rod-shaped, colony-forming
description: Bacillus wiedmannii DSM 102050 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from raw milk stored in a dairy powder processing plant.
NCBI tax id
- NCBI tax id: 1890302
- Matching level: species
strain history
@ref | history |
---|---|
24800 | <- K. Boor, Cornell Univerity <- Matthew Watterson, Cornell University, Ithaca, USA; FSL W8-0169 |
123949 | CIP <- 2017, DSMZ <- K. Boor, Cornell Univ., Ithaca, NY, USA <- M. Watterson, Cornell Univ., Ithaca, NY, USA: strain FSL W8-0169 |
doi: 10.13145/bacdive133012.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus wiedmannii
- full scientific name: Bacillus wiedmannii Miller et al. 2016
@ref: 24800
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus wiedmannii
full scientific name: Bacillus wiedmannii Miller et al. 2016
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
25027 | positive | 2.8 µm | 1.2 µm | rod-shaped | |
123949 | positive | rod-shaped | no |
colony morphology
- @ref: 25027
- colony color: creamy
- colony shape: circular
- incubation period: 1 day
- medium used: BHI agar (brain heart infusion medium, Becton Dickinson)
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
25027 | BHI agar | yes | ||
24800 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
24800 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
123949 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
25027 | positive | growth | 5-43 | |
25027 | positive | optimum | 20-40 | |
24800 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 25027
- oxygen tolerance: facultative anaerobe
spore formation
- @ref: 25027
- spore description: ellipsoidal, present in the centre of vegetative cells, non-swollen sporangia
- type of spore: endospore
- spore formation: yes
compound production
- @ref: 24800
- compound: haemolysin
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
25027 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
25027 | 17426 | 5-dehydro-D-gluconate | - | builds acid from |
25027 | 15963 | ribitol | - | builds acid from |
25027 | 17108 | D-arabinose | - | builds acid from |
25027 | 18333 | D-arabitol | - | builds acid from |
25027 | 28847 | D-fucose | - | builds acid from |
25027 | 62318 | D-lyxose | - | builds acid from |
25027 | 16899 | D-mannitol | - | builds acid from |
25027 | 16024 | D-mannose | - | builds acid from |
25027 | 17924 | D-sorbitol | - | builds acid from |
25027 | 16443 | D-tagatose | - | builds acid from |
25027 | 65327 | D-xylose | - | builds acid from |
25027 | 17113 | erythritol | - | builds acid from |
25027 | 16813 | galactitol | - | builds acid from |
25027 | 28260 | galactose | - | builds acid from |
25027 | 28066 | gentiobiose | - | builds acid from |
25027 | 17754 | glycerol | - | builds acid from |
25027 | 17268 | myo-inositol | - | builds acid from |
25027 | 15443 | inulin | - | builds acid from |
25027 | 30849 | L-arabinose | - | builds acid from |
25027 | 18403 | L-arabitol | - | builds acid from |
25027 | 18287 | L-fucose | - | builds acid from |
25027 | 62345 | L-rhamnose | - | builds acid from |
25027 | 17266 | L-sorbose | - | builds acid from |
25027 | 65328 | L-xylose | - | builds acid from |
25027 | 6731 | melezitose | - | builds acid from |
25027 | 28053 | melibiose | - | builds acid from |
25027 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
25027 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
25027 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
25027 | 16634 | raffinose | - | builds acid from |
25027 | 17992 | sucrose | - | builds acid from |
25027 | 27897 | tryptophan | - | energy source |
25027 | 17151 | xylitol | - | builds acid from |
25027 | 18305 | arbutin | + | builds acid from |
25027 | casein | + | hydrolysis | |
25027 | 17057 | cellobiose | + | builds acid from |
25027 | 15824 | D-fructose | + | builds acid from |
25027 | 17634 | D-glucose | + | builds acid from |
25027 | 4853 | esculin | + | builds acid from |
25027 | 5291 | gelatin | + | builds acid from |
25027 | 28087 | glycogen | + | builds acid from |
25027 | 17306 | maltose | + | builds acid from |
25027 | 506227 | N-acetylglucosamine | + | builds acid from |
25027 | 33942 | ribose | + | builds acid from |
25027 | 17814 | salicin | + | builds acid from |
25027 | 28017 | starch | + | hydrolysis |
25027 | 27082 | trehalose | + | builds acid from |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
25027 | 35581 | indole | no |
25027 | 15688 | acetoin | yes |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
25027 | 15688 | acetoin | + | |
25027 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
25027 | arginine dihydrolase | - | 3.5.3.6 |
25027 | lecithinase | + | |
25027 | lysine decarboxylase | - | 4.1.1.18 |
25027 | ornithine decarboxylase | - | 4.1.1.17 |
25027 | tryptophan deaminase | - | 4.1.99.1 |
25027 | urease | - | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location | isolation date |
---|---|---|---|---|---|---|
24800 | raw milk stored in a dairy powder processing plant | USA | USA | North America | ||
25027 | silo raw milk sample collected from a dairy powder processing plant | north-eastern USA | 2012 | |||
123949 | Animal, Raw milk stored in a dairy powder processing plant | United States of America | USA | North America | North-eastern USA |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host Body Product | #Fluids | #Milk |
#Engineered | #Food production | #Dairy product |
#Engineered | #Industrial | #Plant (Factory) |
taxonmaps
- @ref: 69479
- File name: preview.99_26.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_21;97_22;98_25;99_26&stattab=map
- Last taxonomy: Bacillus
- 16S sequence: KU198626
- Sequence Identity:
- Total samples: 4969
- soil counts: 1920
- aquatic counts: 446
- animal counts: 1524
- plant counts: 1079
Safety information
risk assessment
- @ref: 123949
- biosafety level: 1
- biosafety level comment: Risk group (French classification)
Sequence information
16S sequences
@ref | description | accession | database | length | NCBI tax ID |
---|---|---|---|---|---|
25027 | 16S rRNA | SRX1297474 | nuccore | ||
24800 | Bacillus wiedmannii strain FSL W8-0169 16S ribosomal RNA gene, partial sequence | KU198626 | ena | 1540 | 1890302 |
GC content
@ref | GC-content | method |
---|---|---|
25027 | 35.3 | genome sequence analysis |
24800 | 35.3 | sequence analysis |
External links
@ref: 24800
culture collection no.: DSM 102050, CIP 111364, LMG 29269, FSL W8-0169
straininfo link
- @ref: 91286
- straininfo: 404864
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 27520992 | Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments. | Miller RA, Beno SM, Kent DJ, Carroll LM, Martin NH, Boor KJ, Kovac J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001421 | 2016 | Bacillus/*classification/genetics/isolation & purification, Bacillus cereus/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Dairy Products/*microbiology, Dairying, Fatty Acids/chemistry, HeLa Cells, Humans, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, United States | Pathogenicity |
Phylogeny | 31750261 | Characterization of the Genome Feature and Toxic Capacity of a Bacillus wiedmannii Isolate From the Hydrothermal Field in Okinawa Trough. | Zhao Y, Chen C, Gu HJ, Zhang J, Sun L | Front Cell Infect Microbiol | 10.3389/fcimb.2019.00370 | 2019 | Animals, Bacillus/*genetics/isolation & purification, Environmental Microbiology, Female, Fishes/microbiology, *Genome, Bacterial, *Genomics/methods, Hydrothermal Vents/*microbiology, Japan, Mice, Phylogeny, RNA, Ribosomal, 16S/genetics, Virulence/genetics, Whole Genome Sequencing | Genetics |
Phylogeny | 32155116 | Bacillus fungorum sp. nov., a bacterium isolated from spent mushroom substrate. | Liu X, Wang L, Han M, Xue QH, Zhang GS, Gao J, Sun X | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003673 | 2020 | *Agaricales, Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24800 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-102050 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102050) | |||
25027 | R. A. B. Miller, S. M.,Kent, D. J.,Carroll, L. M.,Martin, N. H.,Boor, K. J.,Kovac, J. | Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments | 10.1099/ijsem.0.001421 | IJSEM 66: 4744-4753 2016 | 27520992 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
91286 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID404864.1 | |||
123949 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111364 | Collection of Institut Pasteur (CIP 111364) |