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Strain identifier

BacDive ID: 133012

Type strain: Yes

Species: Bacillus wiedmannii

Strain history: <- K. Boor, Cornell Univerity <- Matthew Watterson, Cornell University, Ithaca, USA; FSL W8-0169

NCBI tax ID(s): 1890302 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 24800

BacDive-ID: 133012

DSM-Number: 102050

keywords: rod-shaped, Gram-positive, spore-forming, facultative anaerobe, Bacteria, 16S sequence, colony-forming

description: Bacillus wiedmannii DSM 102050 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from raw milk stored in a dairy powder processing plant.

NCBI tax id

  • NCBI tax id: 1890302
  • Matching level: species

strain history: <- K. Boor, Cornell Univerity <- Matthew Watterson, Cornell University, Ithaca, USA; FSL W8-0169

doi: 10.13145/bacdive133012.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus wiedmannii
  • full scientific name: Bacillus wiedmannii Miller et al. 2016

@ref: 24800

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus wiedmannii

full scientific name: Bacillus wiedmannii Miller et al. 2016

type strain: yes

Morphology

cell morphology

  • @ref: 25027
  • gram stain: positive
  • cell length: 2.8 µm
  • cell width: 1.2 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 25027
  • colony color: creamy
  • colony shape: circular
  • incubation period: 1 day
  • medium used: BHI agar (brain heart infusion medium, Becton Dickinson)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25027BHI agaryes
24800NUTRIENT AGAR (DSMZ Medium 1)yeshttps://bacmedia.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
24800COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
25027positivegrowth5-43
25027positiveoptimum20-40
24800positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 25027
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 25027
  • spore description: ellipsoidal, present in the centre of vegetative cells, non-swollen sporangia
  • type of spore: endospore
  • spore formation: yes

compound production

  • @ref: 24800
  • compound: haemolysin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
25027168082-dehydro-D-gluconate-builds acid from
25027174265-dehydro-D-gluconate-builds acid from
2502715963ribitol-builds acid from
2502717108D-arabinose-builds acid from
2502718333D-arabitol-builds acid from
2502728847D-fucose-builds acid from
2502762318D-lyxose-builds acid from
2502716899D-mannitol-builds acid from
2502716024D-mannose-builds acid from
2502717924D-sorbitol-builds acid from
2502716443D-tagatose-builds acid from
2502765327D-xylose-builds acid from
2502717113erythritol-builds acid from
2502716813galactitol-builds acid from
2502728260galactose-builds acid from
2502728066gentiobiose-builds acid from
2502717754glycerol-builds acid from
2502717268myo-inositol-builds acid from
2502715443inulin-builds acid from
2502730849L-arabinose-builds acid from
2502718403L-arabitol-builds acid from
2502718287L-fucose-builds acid from
2502762345L-rhamnose-builds acid from
2502717266L-sorbose-builds acid from
2502765328L-xylose-builds acid from
250276731melezitose-builds acid from
2502728053melibiose-builds acid from
25027320061methyl alpha-D-glucopyranoside-builds acid from
2502743943methyl alpha-D-mannoside-builds acid from
2502774863methyl beta-D-xylopyranoside-builds acid from
2502716634raffinose-builds acid from
2502717992sucrose-builds acid from
2502727897tryptophan-energy source
2502717151xylitol-builds acid from
2502718305arbutin+builds acid from
25027casein+hydrolysis
2502717057cellobiose+builds acid from
2502715824D-fructose+builds acid from
2502717634D-glucose+builds acid from
250274853esculin+builds acid from
250275291gelatin+builds acid from
2502728087glycogen+builds acid from
2502717306maltose+builds acid from
25027506227N-acetylglucosamine+builds acid from
2502733942ribose+builds acid from
2502717814salicin+builds acid from
2502728017starch+hydrolysis
2502727082trehalose+builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
2502735581indoleno
2502715688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2502715688acetoin+
2502735581indole-

enzymes

@refvalueactivityec
25027arginine dihydrolase-3.5.3.6
25027lecithinase+
25027lysine decarboxylase-4.1.1.18
25027ornithine decarboxylase-4.1.1.17
25027tryptophan deaminase-4.1.99.1
25027urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
24800raw milk stored in a dairy powder processing plantUSAUSANorth America
25027silo raw milk sample collected from a dairy powder processing plantnorth-eastern USA2012

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Milk
#Engineered#Food production#Dairy product
#Engineered#Industrial#Plant (Factory)

Sequence information

16S sequences

@refdescriptionaccessiondatabaselengthNCBI tax ID
2502716S rRNASRX1297474nuccore
24800Bacillus wiedmannii strain FSL W8-0169 16S ribosomal RNA gene, partial sequenceKU198626ena15401890302

GC content

@refGC-contentmethod
2502735.3genome sequence analysis
2480035.3sequence analysis

External links

@ref: 24800

culture collection no.: DSM 102050, CIP 111364, LMG 29269, FSL W8-0169

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27520992Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments.Miller RA, Beno SM, Kent DJ, Carroll LM, Martin NH, Boor KJ, Kovac JInt J Syst Evol Microbiol10.1099/ijsem.0.0014212016Bacillus/*classification/genetics/isolation & purification, Bacillus cereus/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Dairy Products/*microbiology, Dairying, Fatty Acids/chemistry, HeLa Cells, Humans, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, United StatesPathogenicity
Phylogeny31750261Characterization of the Genome Feature and Toxic Capacity of a Bacillus wiedmannii Isolate From the Hydrothermal Field in Okinawa Trough.Zhao Y, Chen C, Gu HJ, Zhang J, Sun LFront Cell Infect Microbiol10.3389/fcimb.2019.003702019Animals, Bacillus/*genetics/isolation & purification, Environmental Microbiology, Female, Fishes/microbiology, *Genome, Bacterial, *Genomics/methods, Hydrothermal Vents/*microbiology, Japan, Mice, Phylogeny, RNA, Ribosomal, 16S/genetics, Virulence/genetics, Whole Genome SequencingGenetics
Phylogeny32155116Bacillus fungorum sp. nov., a bacterium isolated from spent mushroom substrate.Liu X, Wang L, Han M, Xue QH, Zhang GS, Gao J, Sun XInt J Syst Evol Microbiol10.1099/ijsem.0.0036732020*Agaricales, Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24800Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102050Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102050)
25027R. A. B. Miller, S. M.,Kent, D. J.,Carroll, L. M.,Martin, N. H.,Boor, K. J.,Kovac, J.Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments10.1099/ijsem.0.001421IJSEM 66: 4744-4753 201627520992