Campylobacter jejuni subsp. doylei (DSM 104768, ATCC 49349, CCUG 24567)

Name: Campylobacter jejuni subsp. doylei (DSM 104768, ATCC 49349, CCUG 24567)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132841

Type strain

Strain Designation 093, 1141

Culture col. no. DSM 104768 ATCC 49349 CCUG 24567 LMG 8803 NCTC 11951 5 more

NCBI tax ID(s) 1408360 32021

Special Collections DSMZ CCUG CIP

Links

Campylobacter jejuni subsp. doylei 093 is a microaerophile, mesophilic, Gram-negative prokaryote that was isolated from Faeces , 2 year old child.

Gram-negative motile rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 1494

LMG 8843,ATCC 49349,NCTC 11951,DSM 104768,CCUG 24567,CIP 103751

@ref 20215

Last LPSN update 2025-12-16 10:01:37

Domain Pseudomonadati

Phylum Pseudomonadota

Class Epsilonproteobacteria

Order Campylobacterales

Family Campylobacteraceae

Genus Campylobacter

Species Campylobacter jejuni subsp. doylei

Full scientific name Campylobacter jejuni subsp. doylei Steele and Owen 1988

BacDive ID	Other strains from **Campylobacter jejuni subsp. doylei** (6)	Type strain
143287	C. jejuni subsp. doylei CCUG 18265, NCTC 11847, LMG 7790
143288	C. jejuni subsp. doylei CCUG 18266, NCTC 11848, LMG 8556
144676	C. jejuni subsp. doylei CCUG 26152, LMG 7918
144677	C. jejuni subsp. doylei CCUG 26155, LMG 9243
144678	C. jejuni subsp. doylei CCUG 26156, LMG 9255
148857	C. jejuni subsp. doylei CCUG 36506

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	97.955
125439	negative	98.7
120329	negative

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
24629	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
40779	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120329	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24629	positive	growth	37	mesophilic
40779	positive	growth	37	mesophilic
47975	positive	growth	37	mesophilic
47975	negative	growth	42
120329	negative	growth	30
120329	negative	growth	25
120329	negative	growth	10
120329	negative	growth	5
120329	positive	growth	37-41
120329	negative	growth	45

Physiology and metabolism

Oxygen tolerance

47975

Oxygen tolerancemicroaerophile

Spore formation

@ref	Spore formation	Confidence
125438		94.64
125439		96.9

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120329	NaCl	positive	growth	3.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120329	606565 ChEBI	hippurate	-	hydrolysis
68373	606565 ChEBI	hippurate	+	hydrolysis	from API CAM
68373	25115 ChEBI	malate	-	assimilation	from API CAM
120329	17632 ChEBI	nitrate	-	reduction
120329	16301 ChEBI	nitrite	-	reduction
68373	78019 ChEBI	triphenyltetrazolium chloride	+	reduction	from API CAM
68373	16199 ChEBI	urea	-	hydrolysis	from API CAM

Antibiotic resistance

@ref	Metabolite
120329	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
68373	cefazolin	from API CAM
68373	nalidixic acid	from API CAM

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68373	16136 ChEBI	hydrogen sulfide		from API CAM
120329	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68373	alkaline phosphatase	+	3.1.3.1	from API CAM
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120329	catalase	+	1.11.1.6
68373	catalase	+	1.11.1.6	from API CAM
68382	cystine arylamidase	-	3.4.11.3	from API zym
120329	DNase	+
68373	esterase	+		from API CAM
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120329	gamma-glutamyltransferase	+	2.3.2.2
68373	gamma-glutamyltransferase	+	2.3.2.2	from API CAM
120329	gelatinase	-
68373	L-arginine arylamidase	-		from API CAM
68373	L-aspartate arylamidase	-	3.4.11.21	from API CAM
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120329	oxidase	+
68373	pyrrolidonyl arylamidase	-	3.4.19.3	from API CAM
68382	trypsin	-	3.4.21.4	from API zym
120329	urease	-	3.5.1.5
68373	urease	-	3.5.1.5	from API CAM
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120329	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-

API CAM

@ref	URE	Reduction of nitrateNIT	EsteraseEST	HIP	GGT	TTC	PYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	PAL	H2S productionH2S	GLU	SUT	NAL	CFZ	ACE	PROP	MLT	CIT	Erythromycin resistance (+) sensitivity (-)ERO	CAT
24629	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
24629	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
24629	-	+	+	+	+	+	-	-	-	+	-	+	+	-	-	+/-	+/-	-	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Infection	#Patient
#Host Body Product	#Gastrointestinal tract
#Host	#Human

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
24629	Faeces (fluid), 2 year old child	Australia	AUS	Australia and Oceania
47975	Human feces,2-yr-old child	Australia	AUS	Australia and Oceania
120329	Human, Child, feces	Australia	AUS	Australia and Oceania

Taxonmaps

69479

Global distribution of 16S sequence DQ174144 (>99% sequence identity) for Campylobacter jejuni from Microbeatlas

Aquatic Samples	2583
Soil Samples	1150
Animal Samples	42043
Plant Samples	337
Total Samples	46113

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24629	2	Risk group (German classification)
120329	2	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	50377_B01 assembly for Campylobacter jejuni subsp. doylei NCTC11951	complete	GCA_900637255	32021.10	8117547213	32021	67.29
66792	ASM70174v1 assembly for Campylobacter jejuni subsp. doylei ATCC 49349	scaffold	GCA_000701745	1408360.3	2561511059	1408360	44.62

Genome browser: GCA_900637255

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24629	Campylobacter jejuni subsp. doylei strain LMG 8843 16S ribosomal RNA (rrs) gene, partial sequence	DQ174144	1341		32021

GC content

@ref	GC-content (mol%)	Method
24629	28	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Campylobacter jejuni subsp. doylei NCTC11951
NCBI: GCA_900637255

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.90	no
125439	motility	BacteriaNetⓘ	no	65.40	no
125439	gram_stain	BacteriaNetⓘ	negative	98.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	82.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Campylobacter jejuni subsp. doylei NCTC11951
NCBI: GCA_900637255.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.96	no
125438	anaerobic	anaerobicⓘ	no	81.95	yes
125438	aerobic	aerobicⓘ	no	80.40	no
125438	spore-forming	spore-formingⓘ	no	94.64	no
125438	thermophilic	thermophileⓘ	no	90.13	no
125438	flagellated	motile2+ⓘ	yes	63.47	no

Literature

Title	Authors	Journal	DOI	Year
Europium Nanoparticle-Based Lateral Flow Strip Biosensors Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Five Zoonotic Foodborne Pathogens.	Jin B, Ma B, Mei Q, Xu S, Deng X, Hong Y, Li J, Xu H, Zhang M.	Biosensors (Basel)	10.3390/bios13060652	2023
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP).	Zautner AE, Lugert R, Masanta WO, Weig M, Gross U, Bader O.	J Vis Exp	10.3791/54165	2016
Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis.	Zirnstein G, Li Y, Swaminathan B, Angulo F.	J Clin Microbiol	10.1128/jcm.37.10.3276-3280.1999	1999
Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri.	Miltenburg MG, Cloutier M, Craiovan E, Lapen DR, Wilkes G, Topp E, Khan IUH.	BMC Microbiol	10.1186/s12866-020-01826-3	2020
Monoclonal antibodies specific for hippurate hydrolase of Campylobacter jejuni.	Steele M, Gyles C, Chan VL, Odumeru J.	J Clin Microbiol	10.1128/jcm.40.3.1080-1082.2002	2002
Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems.	Odumeru JA, Steele M, Fruhner L, Larkin C, Jiang J, Mann E, McNab WB.	J Clin Microbiol	10.1128/jcm.37.4.944-949.1999	1999
TaqMan PCR for detection of Vibrio cholerae O1, O139, non-O1, and non-O139 in pure cultures, raw oysters, and synthetic seawater.	Lyon WJ.	Appl Environ Microbiol	10.1128/aem.67.10.4685-4693.2001	2001
Glucose Metabolism via the Entner-Doudoroff Pathway in Campylobacter: A Rare Trait that Enhances Survival and Promotes Biofilm Formation in Some Isolates.	Vegge CS, Jansen van Rensburg MJ, Rasmussen JJ, Maiden MC, Johnsen LG, Danielsen M, MacIntyre S, Ingmer H, Kelly DJ.	Front Microbiol	10.3389/fmicb.2016.01877	2016
Identification of nine sequence types of the 16S rRNA genes of Campylobacter jejuni subsp. jejuni isolated from broilers.	Hansson I, Persson M, Svensson L, Engvall EO, Johansson KE.	Acta Vet Scand	10.1186/1751-0147-50-10	2008
Detection of Campylobacter spp. in chicken fecal samples by real-time PCR.	Lund M, Nordentoft S, Pedersen K, Madsen M.	J Clin Microbiol	10.1128/jcm.42.11.5125-5132.2004	2004
Toward an international standard for PCR-based detection of food-borne thermotolerant Campylobacters: assay development and analytical validation.	Lubeck PS, Wolffs P, On SL, Ahrens P, Radstrom P, Hoorfar J.	Appl Environ Microbiol	10.1128/aem.69.9.5664-5669.2003	2003
Species-specific identification of campylobacters by partial 16S rRNA gene sequencing.	Gorkiewicz G, Feierl G, Schober C, Dieber F, Kofer J, Zechner R, Zechner EL.	J Clin Microbiol	10.1128/jcm.41.6.2537-2546.2003	2003
Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species.	Engstrand L, Nguyen AM, Graham DY, el-Zaatari FA.	J Clin Microbiol	10.1128/jcm.30.9.2295-2301.1992	1992
CRISPRimmunity: an interactive web server for CRISPR-associated Important Molecular events and Modulators Used in geNome edIting Tool identifYing.	Zhou F, Yu X, Gan R, Ren K, Chen C, Ren C, Cui M, Liu Y, Gao Y, Wang S, Yin M, Huang T, Huang Z, Zhang F.	Nucleic Acids Res	10.1093/nar/gkad425	2023
Molecular characterization of a Campylobacter jejuni lipoprotein with homology to periplasmic siderophore-binding proteins.	Park SF, Richardson PT.	J Bacteriol	10.1128/jb.177.9.2259-2264.1995	1995
Electrophoretic protein typing of Campylobacter jejuni subspecies "doylei" (nitrate-negative campylobacter-like organisms) from human faeces and gastric mucosa.	Owen RJ, Costas M, Sloss LL.	Eur J Epidemiol	10.1007/bf00148910	1988
Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens.	Lage AP, Godfroid E, Fauconnier A, Burette A, Butzler JP, Bollen A, Glupczynski Y.	J Clin Microbiol	10.1128/jcm.33.10.2752-2756.1995	1995
Reproducibility of tolerance tests that are useful in the identification of campylobacteria.	On SL, Holmes B.	J Clin Microbiol	10.1128/jcm.29.9.1785-1788.1991	1991
Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting.	Giesendorf BA, van Belkum A, Koeken A, Stegeman H, Henkens MH, van der Plas J, Goossens H, Niesters HG, Quint WG.	J Clin Microbiol	10.1128/jcm.31.6.1541-1546.1993	1993
Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products.	Wegmuller B, Luthy J, Candrian U.	Appl Environ Microbiol	10.1128/aem.59.7.2161-2165.1993	1993
Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov.	Wang H, Li Y, Gu Y, Zhou G, Chen X, Zhang X, Shao Z, Zhang J, Zhang M.	Microorganisms	10.3390/microorganisms11040971	2023

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24629	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104768
#40779	; Curators of the CIP;
#47975	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 24567
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68373	Automatically annotated from API CAM .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120329	Collection of Institut Pasteur ; Curators of the CIP; CIP 103751
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Campylobacter jejuni subsp. doylei (DSM 104768, ATCC 49349, CCUG 24567)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API CAM

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900637255

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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