Name: Anaerosporomusa subterranea FRC-RU4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132639

Type strain:

Species: Anaerosporomusa subterranea

Strain Designation: FRC-RU4, RU4

Culture col. no.: DSM 29728, ATCC BAA-2723

Strain history: <- Y. Nathan, Rutgers, State Univ. New Jersey, New Brunswick, USA; RU4 <- M. M. Shah {2014}

NCBI tax ID(s): 1794912 (species)

SI-ID 397048

DSM 29728

Section

Anaerosporomusa subterranea FRC-RU4 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from unconsolidated saprolite from a sediment core.

curved-shaped
Gram-negative
motile
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Selenomonadales*
	Family	*Sporomusaceae*
	Genus	*Anaerosporomusa*
	Species	**Anaerosporomusa subterranea**
	Full Scientific Name (LPSN)	Anaerosporomusa subterranea Choi et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24921]	Gram stain	negative

[Ref.: #24921]	Cell length	2.0-4.0 µm

[Ref.: #24921]	Cell width	0.3-0.5 µm

[Ref.: #24921]	Cell shape	curved-shaped

[Ref.: #24921]	Motility	yes

[Ref.: #24921]	Flagellum arrangement	monotrichous

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24428]

Culture medium

SYNTROPHOBACTER (MPOB) MEDIUM (DSMZ Medium 684)

[Ref.: #24428]

Culture medium growth

[Ref.: #24428]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24428]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24428]	growth	28 °C
[Ref.: #24921]	optimum	28 °C
[Ref.: #24921]	growth	24.0-30.0 °C

[Ref.: #24428]	Temperature range	mesophilic
[Ref.: #24921]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #24921]			optimum	6.8
[Ref.: #24921]			growth	5.0-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24921]

Oxygen tolerance

anaerobe

[Ref.: #24921]	Spore description	formed at late exponential phase, heat-resistant
[Ref.: #24921]	Type of spore	endospore
[Ref.: #24921]	Ability of spore formation	yes

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24921]	citrate ChEBI	-	fermentation
[Ref.: #24921]	ferric oxide ChEBI	-	reduction
[Ref.: #24921]	formate ChEBI	-	fermentation
[Ref.: #24921]	fumarate ChEBI	+	fermentation
[Ref.: #24921]	glucose ChEBI	-	fermentation
[Ref.: #24921]	glycerol ChEBI	-	fermentation
[Ref.: #24921]	lactate ChEBI	-	fermentation
[Ref.: #24921]	maleic acid ChEBI	+	fermentation
[Ref.: #24921]	oxaloacetate ChEBI	-	fermentation
[Ref.: #24921]	pyruvate ChEBI	-	fermentation
[Ref.: #24921]	succinate ChEBI	-	fermentation
[Ref.: #24921]	sulfate ChEBI	-	reduction
	Only first 10 entries are displayed. Click here to see all.

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	isoleucine metabolism	100	8 of 8
[Ref.: #66794]	urea cycle	92.31	12 of 13
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	propionate fermentation	90	9 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	citric acid cycle	85.71	12 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	85.71	12 of 14
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	vitamin B6 metabolism	81.82	9 of 11
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	80	4 of 5
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	ethylmalonyl-CoA pathway	80	4 of 5
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	vitamin B12 metabolism	79.41	27 of 34
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	glutamate and glutamine metabolism	78.57	22 of 28
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	alanine metabolism	75.86	22 of 29
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	degradation of sugar alcohols	75	12 of 16
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	purine metabolism	73.4	69 of 94
[Ref.: #66794]	pyrimidine metabolism	73.33	33 of 45
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	degradation of sugar acids	72	18 of 25
[Ref.: #66794]	heme metabolism	71.43	10 of 14
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	oxidative phosphorylation	70.33	64 of 91
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	4-hydroxyphenylacetate degradation	70	7 of 10
[Ref.: #66794]	leucine metabolism	69.23	9 of 13
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	66.67	6 of 9
[Ref.: #66794]	histidine metabolism	65.52	19 of 29
[Ref.: #66794]	lipid metabolism	64.52	20 of 31
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	arginine metabolism	62.5	15 of 24
[Ref.: #66794]	methionine metabolism	61.54	16 of 26
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	3-chlorocatechol degradation	60	3 of 5
[Ref.: #66794]	coenzyme M biosynthesis	60	6 of 10
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	lysine metabolism	59.52	25 of 42
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	tryptophan metabolism	57.89	22 of 38
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	sulfate reduction	53.85	7 of 13
[Ref.: #66794]	polyamine pathway	52.17	12 of 23
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	myo-inositol biosynthesis	50	5 of 10
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	glutathione metabolism	50	7 of 14
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	tyrosine metabolism	50	7 of 14
[Ref.: #66794]	vitamin E metabolism	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	degradation of pentoses	50	14 of 28
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	46.15	6 of 13
[Ref.: #66794]	allantoin degradation	44.44	4 of 9
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	creatinine degradation	40	2 of 5
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	phenol degradation	35	7 of 20
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	3-phenylpropionate degradation	33.33	5 of 15
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	androgen and estrogen metabolism	25	4 of 16
[Ref.: #66794]	dTDPLrhamnose biosynthesis	25	2 of 8
[Ref.: #66794]	alginate biosynthesis	25	1 of 4
[Ref.: #66794]	methanogenesis from CO2	25	3 of 12
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	23.53	4 of 17
[Ref.: #66794]	phenylpropanoid biosynthesis	23.08	3 of 13
[Ref.: #66794]	ascorbate metabolism	22.73	5 of 22
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24428]	Sample type/isolated from	unconsolidated saprolite from a sediment core
[Ref.: #24428]	Geographic location (country and/or sea, region)	Tennessee, Oak Ridge, United States Department of Energy (DOE) Oak Ridge Field Research Center (ORFRC) site
[Ref.: #24428]	Country	USA
[Ref.: #24428]	Country ISO 3 Code	USA
[Ref.: #24428]	Continent	North America
[Ref.: #24428]	Geographic location	35.9400°/-84.3400°

[Ref.: #24921]	Enrichment culture	medium A (MA)
[Ref.: #24921]	Enrichment culture composition	20 mM NH4Cl, 2.2 mM KH2PO4 , 0.6 mM CaCl2.2H2O, 8 mM MgCl2.6H2O, 10 mM PIPES buffer and 10 ml mineral solution l-1, adjusted to pH 7 using 1 M NaOH
[Ref.: #24921]	Enrichment culture duration	3 days
[Ref.: #24921]	Enrichment culture temperature	30 ̊C
[Ref.: #24921]	Isolation/enrichment procedure	inverted, deoxygenated MA tubes amended with 1.5 % (w/v) Noble agar
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Core sample
#Environmental	#Terrestrial	#Geologic
#Environmental	#Terrestrial	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX268498 (>99% sequence identity) for Anaerosporomusa subterranea subclade from Microbeatlas

Aquatic Samples	737
Soil Samples	537
Animal Samples	6845
Plant Samples	99
Total Samples	8218

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24428]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24428]	Bacterium FRC-RU4 16S ribosomal RNA gene, partial sequence	HM099520	1509	ENA	768474 tax ID
[Ref.: #24921]	Anaerosporomusa subterranea strain RU4 16S ribosomal RNA gene, partial sequence	KX268498	1509	GenBank	1794912 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Anaerosporomusa subterranea RU4	GCA_001611555	contig	GenBank	1794912 tax ID	*
[Ref.: #66792]	Anaerosporomusa subterranea RU4	2724679194	draft	JGI IMG/M	1794912 tax ID	*

[Ref.: #24428]

GC-content

52 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24428]

Culture collection no.

DSM 29728, ATCC BAA-2723

[Ref.: #90952]

SI-ID 397048

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Anaerosporomusa subterranea gen. nov., sp. nov., a spore-forming anaerobe belonging to the class Negativicutes isolated from saprolite.	Choi JK, Shah M, Yee N	Int J Syst Evol Microbiol	10.1099/ijsem.0.001275	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24428

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29728

#24921

J. K. S. Choi, Madhavi,Yee, Nathan: Anaerosporomusa subterranea gen. nov., sp. nov., a spore-forming anaerobe belonging to the class Negativicutes isolated from saprolite. IJSEM 66: 3848 - 3854 2016 ( DOI 10.1099/ijsem.0.001275 , PubMed 27381468 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90952

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397048.1 )

* These data were automatically processed and therefore are not curated

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