Strain identifier
BacDive ID: 132639
Type strain: 
Species: Anaerosporomusa subterranea
Strain Designation: FRC-RU4, RU4
Strain history: <- Y. Nathan, Rutgers, State Univ. New Jersey, New Brunswick, USA; RU4 <- M. M. Shah {2014}
NCBI tax ID(s): 1794912 (species)
version 8.1 (current version)
General
@ref: 24428
BacDive-ID: 132639
DSM-Number: 29728
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-negative, motile, curved-shaped
description: Anaerosporomusa subterranea FRC-RU4 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from unconsolidated saprolite from a sediment core.
NCBI tax id
- NCBI tax id: 1794912
- Matching level: species
strain history
- @ref: 24428
- history: <- Y. Nathan, Rutgers, State Univ. New Jersey, New Brunswick, USA; RU4 <- M. M. Shah {2014}
doi: 10.13145/bacdive132639.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Negativicutes
- order: Selenomonadales
- family: Sporomusaceae
- genus: Anaerosporomusa
- species: Anaerosporomusa subterranea
- full scientific name: Anaerosporomusa subterranea Choi et al. 2016
@ref: 24428
domain: Bacteria
phylum: Firmicutes
class: Negativicutes
order: Selenomonadales
family: Sporomusaceae
genus: Anaerosporomusa
species: Anaerosporomusa subterranea
full scientific name: Anaerosporomusa subterranea Choi et al. 2016
strain designation: FRC-RU4, RU4
type strain: yes
Morphology
cell morphology
- @ref: 24921
- gram stain: negative
- cell length: 2.0-4.0 µm
- cell width: 0.3-0.5 µm
- cell shape: curved-shaped
- motility: yes
- flagellum arrangement: monotrichous
Culture and growth conditions
culture medium
- @ref: 24428
- name: SYNTROPHOBACTER (MPOB) MEDIUM (DSMZ Medium 684)
- growth: yes
- link: https://mediadive.dsmz.de/medium/684
- composition: Name: SYNTROPHOBACTER (MPOB) MEDIUM (DSMZ Medium 684) Composition: Na2-fumarate 3.19043 g/l Na2CO3 1.49551 g/l Na2HPO4 x 2 H2O 0.528415 g/l Na2S x 9 H2O 0.498504 g/l KH2PO4 0.408774 g/l NaCl 0.299103 g/l NH4Cl 0.299103 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.109671 g/l MgCl2 x 6 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 24921 | positive | optimum | 28 | mesophilic |
| 24921 | positive | growth | 24.0-30.0 | |
| 24428 | positive | growth | 28 | mesophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 24921 | positive | optimum | 6.8 |
| 24921 | positive | growth | 5.0-7.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 24921
- oxygen tolerance: anaerobe
spore formation
- @ref: 24921
- spore description: formed at late exponential phase, heat-resistant
- type of spore: endospore
- spore formation: yes
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 24921 | 16947 | citrate | - | fermentation |
| 24921 | 50819 | ferric oxide | - | reduction |
| 24921 | 15740 | formate | - | fermentation |
| 24921 | 17234 | glucose | - | fermentation |
| 24921 | 17754 | glycerol | - | fermentation |
| 24921 | 24996 | lactate | - | fermentation |
| 24921 | 16452 | oxaloacetate | - | fermentation |
| 24921 | 15361 | pyruvate | - | fermentation |
| 24921 | 30031 | succinate | - | fermentation |
| 24921 | 16189 | sulfate | - | reduction |
| 24921 | 29806 | fumarate | + | fermentation |
| 24921 | 18300 | maleic acid | + | fermentation |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature | isolation procedure |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 24428 | unconsolidated saprolite from a sediment core | Tennessee, Oak Ridge, United States Department of Energy (DOE) Oak Ridge Field Research Center (ORFRC) site | USA | USA | North America | 35.94 | -84.34 | |||||
| 24921 | medium A (MA) | 20 mM NH4Cl, 2.2 mM KH2PO4 , 0.6 mM CaCl2.2H2O, 8 mM MgCl2.6H2O, 10 mM PIPES buffer and 10 ml mineral solution l-1, adjusted to pH 7 using 1 M NaOH | 3 days | 30 | inverted, deoxygenated MA tubes amended with 1.5 % (w/v) Noble agar |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Core sample |
| #Environmental | #Terrestrial | #Geologic |
| #Environmental | #Terrestrial | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_2123.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_138;96_1162;97_1366;98_1657;99_2123&stattab=map
- Last taxonomy: Anaerosporomusa subterranea subclade
- 16S sequence: KX268498
- Sequence Identity:
- Total samples: 8218
- soil counts: 537
- aquatic counts: 737
- animal counts: 6845
- plant counts: 99
Safety information
risk assessment
- @ref: 24428
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 24921 | Anaerosporomusa subterranea strain RU4 16S ribosomal RNA gene, partial sequence | KX268498 | 1509 | nuccore | 1794912 |
| 24428 | Bacterium FRC-RU4 16S ribosomal RNA gene, partial sequence | HM099520 | 1509 | ena | 768474 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Anaerosporomusa subterranea RU4 | GCA_001611555 | contig | ncbi | 1794912 |
| 66792 | Anaerosporomusa subterranea RU4 | 2724679194 | draft | img | 1794912 |
GC content
- @ref: 24428
- GC-content: 52
- method: high performance liquid chromatography (HPLC)
External links
@ref: 24428
culture collection no.: DSM 29728, ATCC BAA 2723
straininfo link
- @ref: 90952
- straininfo: 397048
literature
- topic: Phylogeny
- Pubmed-ID: 27381468
- title: Anaerosporomusa subterranea gen. nov., sp. nov., a spore-forming anaerobe belonging to the class Negativicutes isolated from saprolite.
- authors: Choi JK, Shah M, Yee N
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001275
- year: 2016
- mesh: Bacteria, Anaerobic/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Firmicutes/*classification/genetics/isolation & purification, Genes, Bacterial, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Tennessee
- topic2: Enzymology
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 24428 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29728 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29728) | |||
| 24921 | J. K. S. Choi, Madhavi,Yee, Nathan | Anaerosporomusa subterranea gen. nov., sp. nov., a spore-forming anaerobe belonging to the class Negativicutes isolated from saprolite | 10.1099/ijsem.0.001275 | IJSEM 66: 3848-3854 2016 | 27381468 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 90952 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397048.1 |