Amycolatopsis thermoflava (DSM 44574, CIP 106795, IFO 14333)

Name: Amycolatopsis thermoflava (DSM 44574, CIP 106795, IFO 14333)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13239

Type strain

Strain Designation N-1165, T526

Culture col. no. DSM 44574 CIP 106795 IFO 14333 JCM 10669 KCTC 9833 7 more

NCBI tax ID(s) 1068979 84480

Special Collections DSMZ JCM KCTC CIP Wink compendium

Links

Amycolatopsis thermoflava N-1165 is an aerobe, thermophilic, Gram-positive prokaryote that was isolated from soil.

Gram-positive aerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 50682

CIP 106795,JCM 10669,IFO 14333,KCTC 9833,DSM 44574,NBRC 14333,NRRL B-24140,BCRC 16371,CCRC 16371,NCIMB 13756,BCRC 13738

@ref 20215

Last LPSN update 2025-12-16 09:50:02

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Amycolatopsis

Species Amycolatopsis thermoflava

Full scientific name Amycolatopsis thermoflava Chun et al. 1999

Synonyms (1)

Amycolatopsis eurytherma

Morphology

Cell morphology

@ref	Gram stain
67771	positive
123505	positive
125439	positive
125439

Colony morphology

@ref	Colony color	Incubation period	Medium used
21424	Honey yellow (1005)	10-14 days	ISP 2
21424	Ivory (1014)	10-14 days	ISP 3
21424	Ivory (1014)	10-14 days	ISP 4
21424	Broom yellow (1032)	10-14 days	ISP 5
21424	Broom yellow (1032)	10-14 days	ISP 7
21424	Sand yellow (1002)	10-14 days	Suter with tyrosine
21424	Ochre yellow	10-14 days	Suter without tyrosine
123505

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11871	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21424	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21424	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21424	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21424	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21424	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21424	Suter with tyrosine		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21424	Suter without tyrosine		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
39583	MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicum		Distilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g)
123505	CIP Medium 116	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11871	positive	growth	45	thermophilic
39583	positive	growth	45	thermophilic
67770	positive	growth	45	thermophilic
67771	positive	growth	45	thermophilic
123505	positive	growth	25-45
123505	negative	growth	10

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
67771	aerobe
123505	obligate aerobe
125439	obligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
21424	NaCl	positive	maximum	7.5 %
123505	NaCl	positive	growth	0-4 %
123505	NaCl		growth	6 %
123505	NaCl		growth	8 %
123505	NaCl		growth	10 %

Observation

@ref	Observation
67770	quinones: MK-9(H₄)
67771	quinones: MK-9(H_4_)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
21424	22599 ChEBI	arabinose	+
21424	62968 ChEBI	cellulose	+
123505	16947 ChEBI	citrate	-
68379	17634 ChEBI	D-glucose	-	from API Coryne
68379	16899 ChEBI	D-mannitol	-	from API Coryne
68379	16988 ChEBI	D-ribose	-	from API Coryne
68379	65327 ChEBI	D-xylose	-	from API Coryne
123505	4853 ChEBI	esculin	-
68379	4853 ChEBI	esculin	-	from API Coryne
21424	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	-	from API Coryne
21424	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	from API Coryne
123505	606565 ChEBI	hippurate	-
68379	17716 ChEBI	lactose	-	from API Coryne
68379	17306 ChEBI	maltose	-	from API Coryne
21424	37684 ChEBI	mannose	+
21424	17268 ChEBI	myo-inositol	-
68379	17632 ChEBI	nitrate	-	from API Coryne
123505	17632 ChEBI	nitrate	+
123505	17632 ChEBI	nitrate	-
123505	16301 ChEBI	nitrite	-
21424	16634 ChEBI	raffinose	-
21424	26546 ChEBI	rhamnose	+
21424	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	-	from API Coryne
68379	16199 ChEBI	urea	-	from API Coryne
21424	18222 ChEBI	xylose	+

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123505	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
123505	15688 ChEBI	acetoin	-
123505	17234 ChEBI	glucose

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123505	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
123505	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
123505	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase lipase (C 8)	+		from API zym
123505	gamma-glutamyltransferase	+	2.3.2.2
123505	gelatinase	-
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123505	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
123505	ornithine decarboxylase	-	4.1.1.17
123505	oxidase	-
123505	phenylalanine ammonia-lyase	-	4.3.1.24
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
123505	tryptophan deaminase	-
123505	urease	-	3.5.1.5
68379	urease	-	3.5.1.5	from API Coryne

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
21424	-	-	-	+/-	-	-	-	+	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
21424	not determinedn.d.	+	+/-	+	-	+	+/-	-	-	-	+	+/-	-	-	-	-	+	+	-	-
123505	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11871	soil	Hainan Island	China	CHN	Asia
67771	From soil	Hainan Island	China	CHN	Asia
67770	Soil	Hainan Island	China	CHN	Asia
123505	Environment, Soil	Hainan Island	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AF052390 (>99% sequence identity) for Amycolatopsis from Microbeatlas

Aquatic Samples	7
Soil Samples	25
Animal Samples	225
Plant Samples	15
Total Samples	272

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11871	1	Risk group (German classification) According to TRBA 466
21424	L1	German classification According to TRBA 466
123505	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM47326v1 assembly for Amycolatopsis thermoflava N1165	scaffold	GCA_000473265	1068979.3	2512564010	1068979	77.82

Genome browser: GCA_000473265

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Amycolatopsis thermoflava 16S ribosomal RNA gene, partial sequence	AF052390	1495		1068979

GC content

@ref	GC-content (mol%)	Method
67770	75	thermal denaturation, midpoint method (Tm)
67770	71.6	genome sequence analysis
67771	75.0	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Amycolatopsis thermoflava N1165
NCBI: GCA_000473265

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	88.70	no
125439	motility	BacteriaNetⓘ	no	90.40	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.30	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Amycolatopsis thermoflava sp. nov., a novel soil actinomycete from Hainan Island, China.	Chun J, Kim SB, Oh YK, Seong CN, Lee DH, Bae KS, Lee KJ, Kang SO, Hah YC, Goodfellow M	Int J Syst Bacteriol	10.1099/00207713-49-4-1369	1999

References

#11871	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44574
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#21424	Wink, J.: Compendium of Actinobacteria . HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#39583	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123505	Collection of Institut Pasteur ; Curators of the CIP; CIP 106795
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Amycolatopsis thermoflava (DSM 44574, CIP 106795, IFO 14333)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000473265

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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