Name: Amycolatopsis keratiniphila FH 1893
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13240

Type strain:

Species: Amycolatopsis keratiniphila

Strain Designation: FH 1893

Culture col. no.: DSM 44586, CIP 107964, JCM 12671, NRRL B-24206

Strain history: DSM 44586 <-- J. Wink FH 1893 <-- Höchst India Ltd..

NCBI tax ID(s): 208442 (subspecies)

SI-ID 97664

DSM 44586, CIP 107964, NRRL B-24206, JCM 12671

Section

Amycolatopsis keratiniphila FH 1893 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from soil sample.

Gram-positive
spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Amycolatopsis*
	Species	**Amycolatopsis keratiniphila**
	Full Scientific Name (LPSN)	Amycolatopsis keratiniphila Al-Musallam et al. 2003

Synonym

Amycolatopsis keratiniphila subsp. nogabecina

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #20075]	Colony color	Sand yellow (1002)
[Ref.: #20075]	Incubation period	10-14 days
[Ref.: #20075]	Cultivation medium used	ISP 2

[Ref.: #20075]	Colony color	Sand yellow (1002)
[Ref.: #20075]	Incubation period	10-14 days
[Ref.: #20075]	Cultivation medium used	ISP 3

[Ref.: #20075]	Colony color	Beige (1001)
[Ref.: #20075]	Incubation period	10-14 days
[Ref.: #20075]	Cultivation medium used	ISP 4

[Ref.: #20075]	Colony color	Beige (1001)
[Ref.: #20075]	Incubation period	10-14 days
[Ref.: #20075]	Cultivation medium used	ISP 5

[Ref.: #20075]	Colony color	Sand yellow (1002)
[Ref.: #20075]	Incubation period	10-14 days
[Ref.: #20075]	Cultivation medium used	ISP 6

[Ref.: #20075]	Colony color	Beige (1001)
[Ref.: #20075]	Incubation period	10-14 days
[Ref.: #20075]	Cultivation medium used	ISP 7

[Ref.: #20075]	Multicellular complex forming ability	no
[Ref.: #20075]	Medium Name (multicellularity)	ISP 2

[Ref.: #20075]	Multicellular complex forming ability	no
[Ref.: #20075]	Medium Name (multicellularity)	ISP 3

[Ref.: #20075]	Multicellular complex forming ability	no
[Ref.: #20075]	Medium Name (multicellularity)	ISP 4

[Ref.: #20075]	Multicellular complex forming ability	no
[Ref.: #20075]	Medium Name (multicellularity)	ISP 5

[Ref.: #20075]	Multicellular complex forming ability	no
[Ref.: #20075]	Medium Name (multicellularity)	ISP 6

[Ref.: #20075]	Multicellular complex forming ability	no
[Ref.: #20075]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11901]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11901]

Culture medium growth

[Ref.: #11901]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11901]

Culture medium composition

expand / minimize

[Ref.: #20075]

Culture medium

ISP 2

[Ref.: #20075]

Culture medium growth

[Ref.: #20075]

Culture medium composition

expand / minimize

[Ref.: #20075]

Culture medium

ISP 3

[Ref.: #20075]

Culture medium growth

[Ref.: #20075]

Culture medium composition

expand / minimize

[Ref.: #20075]

Culture medium

ISP 4

[Ref.: #20075]

Culture medium growth

[Ref.: #20075]

Culture medium composition

expand / minimize

[Ref.: #20075]

Culture medium

ISP 5

[Ref.: #20075]

Culture medium growth

[Ref.: #20075]

Culture medium composition

expand / minimize

[Ref.: #20075]

Culture medium

ISP 6

[Ref.: #20075]

Culture medium growth

[Ref.: #20075]

Culture medium composition

expand / minimize

[Ref.: #20075]

Culture medium

ISP 7

[Ref.: #20075]

Culture medium growth

[Ref.: #20075]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #11901]	growth	28 °C
[Ref.: #20075]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #11901]	Temperature range	mesophilic
[Ref.: #20075]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %90
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #11901]	Name of produced compound	nogabecin
[Ref.: #20075]	Name of produced compound	Nogabecin (=Actinoidin B).
[Ref.: #67770]	Name of produced compound	Nogabecin

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #20075]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #20075]	cellulose ChEBI	+
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #20075]	fructose ChEBI	+
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E, API Coryne
[Ref.: #20075]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #20075]	mannitol ChEBI	+
[Ref.: #20075]	myo-inositol ChEBI	+
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #20075]	raffinose ChEBI	-
[Ref.: #20075]	rhamnose ChEBI	+
[Ref.: #20075]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E, API Coryne
[Ref.: #20075]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API 20E, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	+	3.2.1.31	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20075]	628	+	+	+	+	+	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #20075]	425	-	-	+	+	+	+	+	+	+	+	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20075]	857	not determinedn.d.	+	-	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11901]	Sample type/isolated from	soil sample
[Ref.: #11901]	Country	India
[Ref.: #11901]	Country ISO 3 Code	IND
[Ref.: #11901]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ508238 (>99% sequence identity) for Amycolatopsis from Microbeatlas

Aquatic Samples	30
Soil Samples	689
Animal Samples	79
Plant Samples	635
Total Samples	1433

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11901]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #20075]	Biosafety level	1	German classification

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11901]	Amycolatopsis nogabecetica 16S rRNA gene, type strain DSM 44586T	AJ508238	1471	ENA	208442 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amycolatopsis keratiniphila subsp. nogabecina FH 1893	GCA_001953855	scaffold	GenBank	208442 tax ID
[Ref.: #66792]	Amycolatopsis keratiniphila FH 1893	GCA_900105855	chromosome	GenBank	129921 tax ID	*
[Ref.: #66792]	Amycolatopsis keratiniphila strain FH 1893	129921.3	complete	PATRIC	129921 tax ID	*
[Ref.: #66792]	Amycolatopsis keratiniphila nogabecina DSM 44586	2634166275	draft	JGI IMG/M	129921 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Amycolatopsis keratiniphila strain FH 1893 PATRIC: 129921.3
[Ref.: #69481]	spore-forming	yes	90	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Amycolatopsis keratiniphila strain FH 1893 PATRIC: 129921.3
[Ref.: #69480]	motile	no	93.44	no
[Ref.: #69480]	gram-positive	yes	85.215	no
[Ref.: #69480]	anaerobic	no	99.501	no
[Ref.: #69480]	aerobic	yes	94.83	no
[Ref.: #69480]	halophile	no	91.289	no
[Ref.: #69480]	spore-forming	yes	93.299	no
[Ref.: #69480]	thermophile	no	98.988	no
[Ref.: #69480]	glucose-util	yes	88.734	no
[Ref.: #69480]	flagellated	no	96.925	no
[Ref.: #69480]	glucose-ferment	no	93.043	no

Availability in culture collections External links

[Ref.: #11901]

Culture collection no.

DSM 44586, CIP 107964, JCM 12671, NRRL B-24206

[Ref.: #82445]

SI-ID 97664

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Amycolatopsis pittospori sp. nov., an endophytic actinobacterium isolated from native apricot tree and genome mining revealed the biosynthesis potential as antibiotic producer and plant growth promoter.	Kaewkla O, Franco CMM	Antonie Van Leeuwenhoek	10.1007/s10482-021-01519-5	2021	*
Phylogeny	Amycolatopsis magusensis sp. nov., isolated from soil.	Camas M, Sahin N, Sazak A, Sproer C, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijs.0.042770-0	2012	*
Phylogeny	Three new antibiotic producing species of the genus Amycolatopsis, Amycolatopsis balhimycina sp. nov., A. tolypomycina sp. nov., A. vancoresmycina sp. nov., and description of Amycolatopsis keratiniphila subsp. keratiniphila subsp. nov. and A. keratiniphila subsp. nogabecina subsp. nov.	Wink JM, Kroppenstedt RM, Ganguli BN, Nadkarni SR, Schumann P, Seibert G, Stackebrandt E	Syst Appl Microbiol	10.1078/072320203322337290	2003	*

References

#11901

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44586

#20075

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82445

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID97664.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Amycolatopsis keratiniphila subsp. keratiniphila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Amycolatopsis keratiniphila strain FH 1893

Amycolatopsis keratiniphila strain FH 1893

Change proposal

Change proposal