Amycolatopsis thermoflava (DSM 44348, NCIMB 13795, NT202)

Name: Amycolatopsis thermoflava (DSM 44348, NCIMB 13795, NT202)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13232

Type strain

Strain Designation NT202

Culture col. no. DSM 44348 NCIMB 13795 NT202 NBRC 104161 NBRC 100338 4 more

NCBI tax ID(s) 84480

Special Collections DSMZ JCM CIP Wink compendium

History <- M. Goodfellow, Univ. of Newcastle, NT202 <- N. Sahin BCRC 16370 <-- DSM 44348 <-- M. Goodfellow NT202. CIP <- 2002, NCIMB <- M. Goodfellow, Newcastle Univ., Newcastle, UK: strain NT202 <- N. Sahin

Links

Amycolatopsis thermoflava NT202 is an obligate aerobe, thermophilic, Gram-negative prokaryote that builds an aerial mycelium and was isolated from scrubland soil sample.

Gram-negative rod-shaped obligate aerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 100501

DSM 44348,NCIMB 13795,JCM 12071,CIP 107587,NBRC 100338,BCRC 16370,CCRC 16370,NBRC 104161,JCM 18060

@ref 20215

Last LPSN update 2025-12-16 09:50:02

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Amycolatopsis

Species Amycolatopsis thermoflava

Full scientific name Amycolatopsis thermoflava Chun et al. 1999

Synonyms (1)

Amycolatopsis eurytherma

BacDive ID	Other strains from **Amycolatopsis thermoflava** (1)	Type strain
13239	A. thermoflava N-1165, T526, DSM 44574, CIP 106795, IFO ... (type strain)

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
119145	negative	rod-shaped
125439	positive		99.9
125438	positive		92.308

Colony morphology

@ref	Colony color	Incubation period	Medium used
19651	Sand yellow	10-14 days	ISP 2
19651	Beige	10-14 days	ISP 3
19651	Beige	10-14 days	ISP 4
19651	Beige	10-14 days	ISP 5
19651	Beige	10-14 days	ISP 6
19651	Beige	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19651	Aerial Mycelium	White	ISP 2
19651			ISP 3
19651			ISP 4
19651	Aerial Mycelium		ISP 5
19651			ISP 6
19651	Aerial Mycelium		ISP 7

Multimedia

@ref:	11693
multimedia content:	DSM_44348.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44348.jpg
caption:	Medium 554 45°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11693	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
11693	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19651	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19651	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19651	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19651	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19651	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19651	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
39905	MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum		Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
119145	CIP Medium 57	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11693	positive	growth	45	thermophilic
19651	positive	optimum	45	thermophilic
39905	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
119145	obligate aerobe
125439	obligate aerobe	99.5

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19651	22599 ChEBI	arabinose	+
19651	62968 ChEBI	cellulose	-
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
19651	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
19651	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
19651	29864 ChEBI	mannitol	+
19651	17268 ChEBI	myo-inositol	+
119145	17632 ChEBI	nitrate	-	reduction
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
119145	16301 ChEBI	nitrite	-	reduction
19651	16634 ChEBI	raffinose	-
19651	26546 ChEBI	rhamnose	+
19651	17992 ChEBI	sucrose	-
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
19651	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119145	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
119145	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
119145	oxidase	-
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
119145	urease	+	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
19651	-	-	-	+	-	-	-	+	+	+	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19651	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	-	-	-	-	-	+	+	-	-
119145	-	+	+	+	+	+	+	+	+	+	+	+	-	+	-	+	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11693	scrubland soil sample
67770	Scrubland soil	Madurai	India	IND	Asia
119145	Environment, Scrubland soil	Madurai	India	IND	Asia

Taxonmaps

69479

Global distribution of 16S sequence AJ000285 (>99% sequence identity) for Amycolatopsis from Microbeatlas

Aquatic Samples	52
Soil Samples	204
Animal Samples	228
Plant Samples	36
Total Samples	520

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11693	1	Risk group (German classification) According to TRBA 466
19651	1	Risk group (German classification) According to TRBA 466
119145	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM375212v1 assembly for Amycolatopsis thermoflava DSM 44348	contig	GCA_003752125	196083.4	2778260928	84480	78.57

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11693	Amycolatopsis sp. DNA for 16S ribosomal RNA	AJ000285	1478		37632

GC content

@ref	GC-content (mol%)	Method
67770	71.8	genome sequence analysis
67770	73	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Amycolatopsis thermoflava DSM 44348
NCBI: GCA_003752125

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	84.30	no
125439	motility	BacteriaNetⓘ	no	87.50	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Amycolatopsis eurytherma strain DSM 44348
PATRIC: 196083.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.31	no
125438	anaerobic	anaerobicⓘ	no	97.68	no
125438	spore-forming	spore-formingⓘ	yes	79.82	no
125438	aerobic	aerobicⓘ	yes	87.59	yes
125438	thermophilic	thermophileⓘ	no	95.99	no
125438	flagellated	motile2+ⓘ	no	86.87	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome-based reclassification of Amycolatopsis eurytherma as a later heterotypic synonym of Amycolatopsis thermoflava.	Singh P, Hira P, Rawat CD, Lal R, Sood U	Int J Syst Evol Microbiol	10.1099/ijsem.0.004642	2021
Phylogeny	Amycolatopsis endophytica sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L.	Miao Q, Qin S, Bian GK, Yuan B, Xing K, Zhang YJ, Li Q, Tang SK, Li WJ, Jiang JH	Antonie Van Leeuwenhoek	10.1007/s10482-011-9588-8	2011
Phylogeny	Amycolatopsis tucumanensis sp. nov., a copper-resistant actinobacterium isolated from polluted sediments.	Albarracin VH, Alonso-Vega P, Trujillo ME, Amoroso MJ, Abate CM	Int J Syst Evol Microbiol	10.1099/ijs.0.010587-0	2009
Phylogeny	Amycolatopsis eurytherma sp. nov., a thermophilic actinomycete isolated from soil.	Kim B, Sahin N, Tan GYA, Zakrzewska-Czerwinska J, Goodfellow M	Int J Syst Evol Microbiol	10.1099/00207713-52-3-889	2002

References

#11693	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44348
#19651	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39905	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119145	Collection of Institut Pasteur ; Curators of the CIP; CIP 107587
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Amycolatopsis thermoflava (DSM 44348, NCIMB 13795, NT202)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Metabolite utilization

Metabolite production

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003752125

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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