Name: Methanobacterium aggregans E09F.3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132210

Type strain:

Species: Methanobacterium aggregans

Strain Designation: E09F.3

Culture col. no.: DSM 29428, JCM 30569

Strain history: M. Rother; Tech. Univ. Dresden, Germany; E09F.3.

NCBI tax ID(s): 1615586 (species)

SI-ID 397770

DSM 29428

Section

Methanobacterium aggregans E09F.3 is a thermophilic archaeon that was isolated from sludge from a commercial biogas plant mainly fed with maize silage, cattle manure and dry poultry faeces.

thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanobacterium*
	Species	**Methanobacterium aggregans**
	Full Scientific Name (LPSN)	Methanobacterium aggregans Kern et al. 2015

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23999]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #23999]

Culture medium growth

[Ref.: #23999]

Culture medium link

Medium recipe at MediaDive

[Ref.: #23999]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #23999]			growth	40 °C
[Ref.: #67770]			growth	40 °C

[Ref.: #23999]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23999]	Sample type/isolated from	sludge from a commercial biogas plant mainly fed with maize silage, cattle manure and dry poultry faeces
[Ref.: #23999]	Geographic location (country and/or sea, region)	near Cologne
[Ref.: #23999]	Country	Germany
[Ref.: #23999]	Country ISO 3 Code	DEU
[Ref.: #23999]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Commercial biogas plant in Germany
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Plant (Factory)
#Environmental	#Terrestrial	#Mud (Sludge)
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP006499 (>99% sequence identity) for Methanobacterium aggregans subclade from Microbeatlas

Aquatic Samples	313
Soil Samples	25
Animal Samples	25
Plant Samples	24
Total Samples	387

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #23999]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23999]	Methanobacterium aggregans strain E09F.3 16S ribosomal RNA gene, partial sequence	KP006499	1411	ENA	1615586 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanobacterium aggregans DSM 29428	GCA_017874455	contig	GenBank	1615586 tax ID	*
[Ref.: #66792]	Methanobacterium aggregans DSM 29428	2913520121	draft	JGI IMG/M	1615586 tax ID	*

[Ref.: #23999]

GC-content

39.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #23999]

Culture collection no.

DSM 29428, JCM 30569

[Ref.: #90563]

SI-ID 397770

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanobacterium aggregans sp. nov., a hydrogenotrophic methanogenic archaeon isolated from an anaerobic digester.	Kern T, Linge M, Rother M	Int J Syst Evol Microbiol	10.1099/ijs.0.000210	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23999

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29428

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90563

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397770.1 )

* These data were automatically processed and therefore are not curated

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