Actinokineospora enzanensis (DSM 44649, IFO 16517, NBRC 16517)

Name: Actinokineospora enzanensis (DSM 44649, IFO 16517, NBRC 16517)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13205

Type strain

Strain Designation YU 924-101

Culture col. no. DSM 44649 IFO 16517 NBRC 16517 YU 924-101 CIP 107895 1 more

NCBI tax ID(s) 1120934 155975

Special Collections DSMZ JCM CIP

Links

Actinokineospora enzanensis YU 924-101 is an obligate aerobe, mesophilic, Gram-positive prokaryote that was isolated from forest soil.

Gram-positive filament-shaped obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 88106

IFO 16517,DSM 44649,JCM 11647,NBRC 16517,CIP 107895

@ref 20215

Last LPSN update 2025-12-16 09:50:02

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Actinokineospora

Species Actinokineospora enzanensis

Full scientific name Actinokineospora enzanensis Otoguro et al. 2003

Morphology

Cell morphology

@ref	Gram stain	Cell shape
119088	positive	filament-shaped
125439	positive
125439

Colony morphology

Multimedia

@ref:	11958
multimedia content:	DSM_44649-Nutrient.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44649-Nutrient.jpg
caption:	Nutrient
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	11958
multimedia content:	DSM_44649.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44649.jpg
caption:	Medium 548 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11958	BENNETT'S AGAR (DSMZ Medium 548)	Medium recipe at MediaDive	Name: BENNETT'S AGAR (DSMZ Medium 548) Composition: Agar 15.0 g/l Glucose 10.0 g/l N-Z amine 2.0 g/l Yeast extract 1.0 g/l Beef extract 1.0 g/l Distilled water
11958	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
34378	MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicum		Distilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g)
119088	CIP Medium 116	Medium recipe at CIP
11958	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11958	positive	growth	28	mesophilic
34378	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic
119088	positive	growth	25-30	mesophilic
119088	negative	growth	10
119088	negative	growth	37
119088	negative	growth	41
119088	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
119088	obligate aerobe
125439	obligate aerobe

Spore formation

@ref	Spore formation	Confidence
125439		96.9

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119088	NaCl	positive	growth	0-2 %
119088	NaCl		growth	4 %
119088	NaCl		growth	6 %
119088	NaCl		growth	8 %
119088	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
119088	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
119088	4853 ChEBI	esculin	-	hydrolysis
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
119088	606565 ChEBI	hippurate	-	hydrolysis
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
119088	17632 ChEBI	nitrate	+	reduction
119088	17632 ChEBI	nitrate	-	respiration
68368	17632 ChEBI	nitrate	-	reduction	from API 20E
119088	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
119088	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	17997 ChEBI	dinitrogen	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
119088	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E
68368	16301 ChEBI	nitrite	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
119088	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	+	from API 20E
119088	17234 ChEBI	glucose
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
119088	alcohol dehydrogenase	-	1.1.1.1
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119088	amylase	+
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
119088	beta-galactosidase	+	3.2.1.23
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119088	caseinase	+	3.4.21.50
119088	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
119088	DNase	-
119088	gamma-glutamyltransferase	+	2.3.2.2
119088	gelatinase	+/-
68368	gelatinase	-		from API 20E
119088	lecithinase	-
119088	lipase	-
68382	lipase (C 14)	-		from API zym
119088	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
119088	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
119088	oxidase	-
119088	phenylalanine ammonia-lyase	-	4.3.1.24
119088	protease	-
68382	trypsin	-	3.4.21.4	from API zym
119088	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
119088	tween esterase	-
119088	urease	-	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
11958	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
11958	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
119088	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11958	forest soil	Yamanashi	Japan	JPN	Asia
67770	Level-land forest soil		Japan	JPN	Asia
119088	Environment, Soil, Level-land forest		Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB058395 (>99% sequence identity) for Actinokineospora enzanensis subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	55
Animal Samples	20
Plant Samples	20
Total Samples	107

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11958	1	Risk group (German classification) According to TRBA 466
119088	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM37444v1 assembly for Actinokineospora enzanensis DSM 44649	scaffold	GCA_000374445	1120934.3	2518285603	1120934	59.2

Genome browser: GCA_000374445

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11958	Actinokineospora enzanensis gene for 16S rRNA, partial sequence	AB058395	1473		155975

GC content

@ref	GC-content (mol%)
11958	70
67770	70

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinokineospora enzanensis DSM 44649
NCBI: GCA_000374445

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	96.90	no
125439	motility	BacteriaNetⓘ	no	96.60	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinokineospora enzanensis DSM 44649
PATRIC: 1120934.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.65	no
125438	anaerobic	anaerobicⓘ	no	96.32	no
125438	spore-forming	spore-formingⓘ	yes	83.54	no
125438	aerobic	aerobicⓘ	yes	87.57	no
125438	thermophilic	thermophileⓘ	no	97.50	yes
125438	flagellated	motile2+ⓘ	no	58.60	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Biosynthetic novelty index reveals the metabolic potential of rare actinobacteria isolated from highly oligotrophic sediments.	Gonzalez-Salazar LA, Quezada M, Rodriguez-Orduna L, Ramos-Aboites H, Capon RJ, Souza-Saldivar V, Barona-Gomez F, Licona-Cassani C.	Microb Genom	10.1099/mgen.0.000921	2023
	A rare peptide scaffold in kineomicins, the glycopeptide antibiotics produced by Actinokineospora auranticolor DSM 44650.	Yushchuk O, Berini F, Zhong L, Ruckert-Reed C, Bernasconi E, Bartolone L, Busche T, Kalinowski J, Sussmuth RD, Marinelli F.	Commun Chem	10.1038/s42004-025-01534-x	2025
	Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium Actinokineospora sp. Strain EG49.	Harjes J, Ryu T, Abdelmohsen UR, Moitinho-Silva L, Horn H, Ravasi T, Hentschel U.	Genome Announc	10.1128/genomea.00160-14	2014
Phylogeny	Alloactinosynnema iranicum sp. nov., a rare actinomycete isolated from a hypersaline wetland, and emended description of the genus Alloactinosynnema.	Nikou MM, Ramezani M, Amoozegar MA, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A.	Int J Syst Evol Microbiol	10.1099/ijs.0.049189-0	2014
Phylogeny	Actinokineospora bangkokensis sp. nov., isolated from rhizospheric soil.	Intra B, Matsumoto A, Inahashi Y, Omura S, Takahashi Y, Panbangred W	Int J Syst Evol Microbiol	10.1099/ijs.0.047928-0	2013

References

#11958	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44649
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34378	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119088	Collection of Institut Pasteur ; Curators of the CIP; CIP 107895
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinokineospora enzanensis (DSM 44649, IFO 16517, NBRC 16517)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000374445

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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