Name: Actinokineospora enzanensis YU 924-101
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13205

Type strain:

Species: Actinokineospora enzanensis

Strain Designation: YU 924-101

Culture col. no.: DSM 44649, IFO 16517, NBRC 16517, JCM 11647, CIP 107895

Strain history: CIP <- 2003, NBRC <- M. Hayakawa, Yamanashi Univ., Kofu, Yamanashi, Japan: strain YU 924-101

NCBI tax ID(s): 1120934 (strain), 155975 (species)

SI-ID 88106

IFO 16517, DSM 44649, JCM 11647, NBRC 16517, CIP 107895

Special Collections

Section

Actinokineospora enzanensis YU 924-101 is an obligate aerobe, spore-forming, Gram-positive bacterium that was isolated from forest soil.

filament-shaped
Gram-positive
spore-forming
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinokineospora*
	Species	**Actinokineospora enzanensis**
	Full Scientific Name (LPSN)	Actinokineospora enzanensis Otoguro et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #119088]	Gram stain	positive

[Ref.: #119088]	Cell shape	filament-shaped

[Ref.: #119088]	Motility	no

Multimedia content

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Caption:

Nutrient

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Intellectual property rights:

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Caption:

Medium 548 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BENNETT'S AGAR (DSMZ Medium 548)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

[Ref.: #34378]

Culture medium

MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicum

[Ref.: #34378]

Culture medium growth

[Ref.: #34378]

Culture medium composition

expand / minimize

[Ref.: #119088]

Culture medium

CIP Medium 116

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11958]		growth	28 °C
[Ref.: #34378]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #119088]		growth	25-30 °C
[Ref.: #119088]	no	growth	10 °C
[Ref.: #119088]	no	growth	37 °C
[Ref.: #119088]	no	growth	41 °C
[Ref.: #119088]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119088]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]

Ability of spore formation

yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #119088]	NaCl		growth	0-2	%
[Ref.: #119088]	NaCl	no	growth	4	%
[Ref.: #119088]	NaCl	no	growth	6	%
[Ref.: #119088]	NaCl	no	growth	8	%
[Ref.: #119088]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #119088]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #119088]	esculin ChEBI	-	hydrolysis
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #119088]	hippurate ChEBI	-	hydrolysis
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #119088]	nitrate ChEBI	+	reduction
[Ref.: #119088]	nitrate ChEBI	-	respiration
[Ref.: #68368]	nitrate ChEBI	-	reduction	* from API 20E
[Ref.: #119088]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119088]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite	Production
[Ref.: #119088]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	dinitrogen ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E
[Ref.: #68368]	nitrite ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #119088]	acetoin ChEBI	-
[Ref.: #119088]	glucose ChEBI		-
[Ref.: #68368]	acetoin ChEBI	+			* from API 20E
[Ref.: #68368]	indole ChEBI			-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #119088]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119088]	amylase	+
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #119088]	beta-galactosidase	+	3.2.1.23
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E, API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119088]	caseinase	+	3.4.21.50
[Ref.: #119088]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #119088]	DNase	-
[Ref.: #119088]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #119088]	gelatinase	+/-
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #119088]	lecithinase	-
[Ref.: #119088]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119088]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #119088]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #119088]	oxidase	-
[Ref.: #119088]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #119088]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119088]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #119088]	tween esterase	-
[Ref.: #119088]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #11958]	17817	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #11958]	14041	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #119088]	10729	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11958]	Sample type/isolated from	forest soil
[Ref.: #11958]	Geographic location (country and/or sea, region)	Yamanashi
[Ref.: #11958]	Country	Japan
[Ref.: #11958]	Country ISO 3 Code	JPN
[Ref.: #11958]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Level-land forest soil
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #119088]	Sample type/isolated from	Environment, Soil, Level-land forest
[Ref.: #119088]	Country	Japan
[Ref.: #119088]	Country ISO 3 Code	JPN
[Ref.: #119088]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB058395 (>99% sequence identity) for Actinokineospora enzanensis subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	55
Animal Samples	20
Plant Samples	20
Total Samples	107

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11958]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119088]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11958]	Actinokineospora enzanensis gene for 16S rRNA, partial sequence	AB058395	1473	GenBank ENA	155975 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinokineospora enzanensis DSM 44649	GCA_000374445	scaffold	GenBank ENA	1120934 tax ID
[Ref.: #66792]	Actinokineospora enzanensis DSM 44649	1120934.3	wgs	PATRIC	1120934 tax ID	*
[Ref.: #66792]	Actinokineospora enzanensis DSM 44649	2518285603	draft	JGI IMG/M	1120934 tax ID	*

[Ref.: #11958]	GC-content	70 mol%
[Ref.: #67770]	GC-content	70 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinokineospora enzanensis DSM 44649 DSM 44649 NCBI: GCA_000374445.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinokineospora enzanensis DSM 44649 PATRIC: 1120934.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	86.653	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.323	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	83.539	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	87.566	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.5	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	58.6	no

Availability in culture collections External links

[Ref.: #11958]

Culture collection no.

DSM 44649, IFO 16517, NBRC 16517, JCM 11647, CIP 107895

[Ref.: #82413]

SI-ID 88106

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinokineospora bangkokensis sp. nov., isolated from rhizospheric soil.	Intra B, Matsumoto A, Inahashi Y, Omura S, Takahashi Y, Panbangred W	Int J Syst Evol Microbiol	10.1099/ijs.0.047928-0	2013	*

References

#11958

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44649

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#34378 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82413

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID88106.1 )

#119088 Collection of Institut Pasteur ; Curators of the CIP; CIP 107895
* These data were automatically processed and therefore are not curated

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Actinokineospora enzanensis DSM 44649 DSM 44649

Actinokineospora enzanensis DSM 44649

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