Name: Corynebacterium crudilactis JZ16
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 131684

Type strain:

Species: Corynebacterium crudilactis

Strain Designation: JZ16

Culture col. no.: DSM 100882, CCUG 69192, LMG 29813

Strain history: <- A. Lipski, Universität Bonn; JZ16 <- J. Zimmermann, Universität Bonn

NCBI tax ID(s): 1652495 (species)

SI-ID 397611

DSM 100882, CCUG 69192

Special Collections

Section

Corynebacterium crudilactis JZ16 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from raw cow's milk.

Gram-positive
rod-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium crudilactis**
	Full Scientific Name (LPSN)	Corynebacterium crudilactis Zimmermann et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #25085]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %91.205

[Ref.: #25085]	Cell length	0.9-1.8 µm

[Ref.: #25085]	Cell width	0.6-1.3 µm

[Ref.: #25085]	Cell shape	rod-shaped

[Ref.: #25085]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90.5

[Ref.: #25085]	Colony size	3 mm
[Ref.: #25085]	Colony color	beige
[Ref.: #25085]	Cultivation medium used	TSA (Trypticase soy agar)

[Ref.: #63554]	Incubation period	1 day

Multimedia content

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Caption:

Medium 693 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

brain-heart infusion agar

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

TSA (Trypticase soy agar)

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Culture medium growth

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Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #23480]			growth	28 °C
[Ref.: #63554]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25085]	Oxygen tolerance	anaerobe
[Ref.: #63554]	Oxygen tolerance	aerobe

[Ref.: #25085]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25085]	D-glucose ChEBI	+	builds acid from
[Ref.: #25085]	D-mannitol ChEBI	+	builds acid from
[Ref.: #25085]	D-ribose ChEBI	+	builds acid from
[Ref.: #25085]	D-xylose ChEBI	-	builds acid from
[Ref.: #25085]	gelatin ChEBI	-	hydrolysis
[Ref.: #25085]	glycogen ChEBI	-	builds acid from
[Ref.: #25085]	lactose ChEBI	-	builds acid from
[Ref.: #25085]	maltose ChEBI	-	builds acid from
[Ref.: #25085]	maltose ChEBI	-	fermentation
[Ref.: #25085]	mannitol ChEBI	+	fermentation
[Ref.: #25085]	nitrate ChEBI	+	reduction
[Ref.: #25085]	sucrose ChEBI	-	builds acid from
[Ref.: #25085]	sucrose ChEBI	-	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #25085]	ampicillin ChEBI	yes	10	µg
[Ref.: #25085]	kanamycin ChEBI	yes	30	µg
[Ref.: #25085]	oxacillin ChEBI	yes	1	µg
[Ref.: #25085]	streptomycin ChEBI	yes	10	µg

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic resistance	Concentration
[Ref.: #25085]		25	sulfamerazine ChEBI	yes	23.75	µg
[Ref.: #25085]		25	trimethoprim ChEBI	yes	1.25	µg

	Enzyme	Enzyme activity	EC number
[Ref.: #25085]	acid phosphatase	-	3.1.3.2
[Ref.: #25085]	alkaline phosphatase	-	3.1.3.1
[Ref.: #25085]	alpha-glucosidase	-	3.2.1.20
[Ref.: #25085]	beta-galactosidase	-	3.2.1.23
[Ref.: #25085]	beta-glucosidase	+	3.2.1.21
[Ref.: #25085]	beta-glucuronidase	+	3.2.1.31
[Ref.: #25085]	catalase	+	1.11.1.6
[Ref.: #25085]	cytochrome oxidase	-	1.9.3.1
[Ref.: #25085]	esterase (C 4)	+
[Ref.: #25085]	esterase Lipase (C 8)	+
[Ref.: #25085]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25085]	pyrazinamidase	+	3.5.1.B15
[Ref.: #25085]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #25085]	trypsin	-	3.4.21.4
[Ref.: #25085]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23480]	Sample type/isolated from	raw cow's milk
[Ref.: #23480]	Geographic location (country and/or sea, region)	Schlausenbach
[Ref.: #23480]	Country	Germany
[Ref.: #23480]	Country ISO 3 Code	DEU
[Ref.: #23480]	Continent	Europe

[Ref.: #25085]	Sample type/isolated from	raw cow ’s milk from the bulk tank of a dairy farm
[Ref.: #25085]	Enrichment culture	brain-heart infusion agar (Oxoid)
[Ref.: #25085]	Enrichment culture composition	supplemented with 0.25 g l-1 potassium tellurite and 1.0 % Tween 80
[Ref.: #25085]	Enrichment culture temperature	30 ̊C

[Ref.: #63554]	Sample type/isolated from	Raw milk from cow
[Ref.: #63554]	Sampling date	2014-11-01
[Ref.: #63554]	Geographic location (country and/or sea, region)	Rheinland-Pfalz,Schlausenbach
[Ref.: #63554]	Country	Germany
[Ref.: #63554]	Country ISO 3 Code	DEU
[Ref.: #63554]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Container (Reservoir)
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

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Global distribution of 16S sequence KR534194 (>99% sequence identity) for Corynebacterium crudilactis from Microbeatlas

Aquatic Samples	1445
Soil Samples	2143
Animal Samples	9718
Plant Samples	443
Total Samples	13749

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23480]	Corynebacterium crudilactis strain JZ16 16S ribosomal RNA gene, partial sequence	KR534194	1443	GenBank ENA	1652495 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Corynebacterium crudilactis JZ16	GCA_001643015	complete	GenBank ENA	1652495 tax ID	*
[Ref.: #66792]	Corynebacterium crudilactis strain JZ16	1652495.6	plasmid	PATRIC	1652495 tax ID	*
[Ref.: #66792]	Corynebacterium crudilactis strain JZ16	1652495.5	plasmid	PATRIC	1652495 tax ID	*
[Ref.: #66792]	Corynebacterium crudilactis JZ16	2718217777	complete	JGI IMG/M	1652495 tax ID	*

[Ref.: #25085]	GC-content	51.59 mol%	genome sequence analysis
[Ref.: #23480]	GC-content	51.59 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium crudilactis JZ16 NCBI: GCA_001643015.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium crudilactis JZ16 NCBI: GCA_001643015.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	91.205	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.086	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	80.211	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	76.216	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.6	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90.5	yes

Availability in culture collections External links

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Culture collection no.

DSM 100882, CCUG 69192, LMG 29813

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SI-ID 397611

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Corynebacterium crudilactis sp. nov., isolated from raw cow's milk.	Zimmermann J, Ruckert C, Kalinowski J, Lipski A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001509	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23480

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100882

#25085

J. R. Zimmermann, C.,Kalinowski, J.,Lipski, A.: Corynebacterium crudilactis sp. nov., isolated from raw cow's milk. IJSEM 66: 5288 - 5293 2016 ( DOI 10.1099/ijsem.0.001509 , PubMed 27666312 )

#63554 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 69192

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90080

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397611.1 )

* These data were automatically processed and therefore are not curated

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