Name: Methanocalculus alkaliphilus AMF2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 131457

Type strain:

Species: Methanocalculus alkaliphilus

Strain Designation: AMF2

Culture col. no.: DSM 24457, UNIQEM U859

Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; AMF2 <- D. Y. Sorokin {2010}

NCBI tax ID(s): 768730 (species)

SI-ID 399576

DSM 24457

Special Collections

DSMZ

Section

Methanocalculus alkaliphilus AMF2 is an anaerobe archaeon that was isolated from anoxic sediments from hypersaline soda lakes.

anaerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanomicrobiales*
	Family	*Methanocalculaceae*
	Genus	*Methanocalculus*
	Species	**Methanocalculus alkaliphilus**
	Full Scientific Name (LPSN)	Methanocalculus alkaliphilus Sorokin et al. 2015

Information on morphological and physiological properties Morphology

[Ref.: #22684]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22684]

Culture medium

METHANOCALCULUS ALKALIPHILUS MEDIUM (DSMZ Medium 1596)

[Ref.: #22684]

Culture medium growth

[Ref.: #22684]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22684]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #22684]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22684]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22684]	Sample type/isolated from	anoxic sediments from hypersaline soda lakes
[Ref.: #22684]	Geographic location (country and/or sea, region)	South-East Siberia, Altai, Kulunda Steppe
[Ref.: #22684]	Country	Russia
[Ref.: #22684]	Country ISO 3 Code	RUS
[Ref.: #22684]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Anoxic (anaerobic)	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HM053969 (>99% sequence identity) for Methanocalculus alkaliphilus subclade from Microbeatlas

Aquatic Samples	28
Soil Samples
Animal Samples
Plant Samples
Total Samples	28

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22684]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22684]	Methanocalculus alkaliphilus strain AMF2 16S ribosomal RNA gene, partial sequence	HM053969	1433	GenBank ENA	768730 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanocalculus alkaliphilus AMF2	2926682867	draft	JGI IMG/M	768730 tax ID	*
[Ref.: #66792]	Methanocalculus alkaliphilus AMF2	GCA_024170505	contig	GenBank ENA	768730 tax ID	*

[Ref.: #22684]

GC-content

51.1 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #22684]

Culture collection no.

DSM 24457, UNIQEM U859

[Ref.: #89877]

SI-ID 399576

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanosalsum natronophilum sp. nov., and Methanocalculus alkaliphilus sp. nov., haloalkaliphilic methanogens from hypersaline soda lakes.	Sorokin DY, Abbas B, Merkel AY, Rijpstra WIC, Damste JSS, Sukhacheva MV, van Loosdrecht MCM	Int J Syst Evol Microbiol	10.1099/ijsem.0.000488	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22684

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24457

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#89877

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399576.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Methanocalculus alkaliphilus in external resources:
SILVA
BRENDA
PANGAEA
GBIF