Name: Aetherobacter fasciculatus SBSr002
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 131429

Type strain:

Species: Aetherobacter fasciculatus

Strain Designation: SBSr002

Culture col. no.: DSM 24601, NCCB 100377

Strain history: <- M. Stadler, InterMed Discovery GmbH, Dortmund, Germany, SBSr002 <- R. Garcia, Univ. Saarland, Germany

NCBI tax ID(s): 888830 (species)

SI-ID 405449

DSM 24601

Special Collections

Section

Aetherobacter fasciculatus SBSr002 is an aerobe, Gram-negative, motile bacterium that was isolated from dried soil with decaying plant material.

Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Deltaproteobacteria*
	Order	*Myxococcales*
	Family	*Polyangiaceae*
	Genus	*Aetherobacter*
	Species	**Aetherobacter fasciculatus**
	Full Scientific Name (LPSN)	Aetherobacter fasciculatus Garcia et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43768]	Gram stain	negative

[Ref.: #43768]	Cell length	2.9-6 µm

[Ref.: #43768]	Cell width	1-1.3 µm

[Ref.: #43768]	Cell shape	rod-shaped

[Ref.: #43768]	Motility	yes

[Ref.: #43768]	Flagellum arrangement	gliding

[Ref.: #43768]	Colony color	Transparent
[Ref.: #43768]	Cultivation medium used	VY/2 agars

[Ref.: #22656]	Incubation period	8-14 days

Multimedia content

[Ref.: #22656]

Caption:

vegetative cells on medium 67

[Ref.: #22656]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43768]

Culture medium

VY/2 AGAR

[Ref.: #43768]

Culture medium growth

[Ref.: #22656]

Culture medium

M1DG-MEDIUM (DSMZ Medium 1296)

[Ref.: #22656]

Culture medium growth

[Ref.: #22656]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22656]

Culture medium composition

expand / minimize

[Ref.: #22656]

Culture medium

CY-AGAR (DSMZ Medium 67)

[Ref.: #22656]

Culture medium growth

[Ref.: #22656]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22656]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #22656]		growth	28 °C
[Ref.: #43768]		growth	22-23 °C
[Ref.: #43768]	no	growth	26 °C
[Ref.: #43768]	no	growth	37 °C
[Ref.: #43768]		optimum	30 °C

	pH	Kind of pH		pH
[Ref.: #43768]			optimum	6-8
[Ref.: #43768]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43768]

Oxygen tolerance

aerobe

[Ref.: #22656]

Name of produced compound

aetheramide A and B

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43768]		NaCl		growth	0-0.1	%(w/v)

[Ref.: #43768]

Observation

Swarm from ring-or halo-like colonies on yeast agar (VY/2)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43768]	casein	+	hydrolysis
[Ref.: #43768]	cellulose ChEBI	-	degradation
[Ref.: #43768]	chitin ChEBI	-	degradation
[Ref.: #43768]	esculin ChEBI	+	hydrolysis
[Ref.: #43768]	glucose ChEBI	+	growth
[Ref.: #43768]	maltose ChEBI	+	growth
[Ref.: #43768]	raffinose ChEBI	+	growth
[Ref.: #43768]	skimmed milk	+	hydrolysis
[Ref.: #43768]	starch ChEBI	+	growth
[Ref.: #43768]	starch ChEBI	+	hydrolysis
[Ref.: #43768]	tryptone	+	growth
[Ref.: #43768]	tween 20 ChEBI	+	hydrolysis
[Ref.: #43768]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43768]	xylan ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance	Concentration
[Ref.: #43768]	ampicillin ChEBI		yes	50	µg/mL
[Ref.: #43768]	apramycin ChEBI		yes	50	µg/mL
[Ref.: #43768]	bacitracin ChEBI		yes	50	µg/mL
[Ref.: #43768]	carbenicillin ChEBI	yes
[Ref.: #43768]	cephalothin ChEBI		yes	50	µg/mL
[Ref.: #43768]	fusidate ChEBI	yes
[Ref.: #43768]	gentamicin ChEBI		yes	50	µg/mL
[Ref.: #43768]	hygromycin ChEBI		yes	50	µg/mL
[Ref.: #43768]	kanamycin ChEBI	yes
[Ref.: #43768]	neomycin ChEBI		yes	50	µg/mL
[Ref.: #43768]	oxytetracycline ChEBI	yes
[Ref.: #43768]	polymyxin ChEBI		yes	50	µg/mL
[Ref.: #43768]	rifampicin ChEBI	yes
[Ref.: #43768]	spectinomycin ChEBI	yes
[Ref.: #43768]	streptomycin ChEBI		yes	50	µg/mL
[Ref.: #43768]	tetracycline ChEBI	yes
[Ref.: #43768]	thiostrepton ChEBI	yes
[Ref.: #43768]	tobramycin ChEBI		yes	50	µg/mL
[Ref.: #43768]	trimethoprim ChEBI		yes	50	µg/mL
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43768]	acid phosphatase	+	3.1.3.2
[Ref.: #43768]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43768]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43768]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43768]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43768]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43768]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43768]	beta-galactosidase	+	3.2.1.23
[Ref.: #43768]	beta-glucosidase	+	3.2.1.21
[Ref.: #43768]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43768]	cystine arylamidase	+	3.4.11.3
[Ref.: #43768]	esterase (C 4)	+
[Ref.: #43768]	esterase Lipase (C 8)	+
[Ref.: #43768]	leucine arylamidase	+	3.4.11.1
[Ref.: #43768]	lipase (C 14)	+/-
[Ref.: #43768]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43768]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43768]	trypsin	-	3.4.21.4
[Ref.: #43768]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22656]	Sample type/isolated from	dried soil with decaying plant material

[Ref.: #43768]	Sample type/isolated from	Dried soil samples from Saarland Centre for Biodocumentation
[Ref.: #43768]	Geographic location (country and/or sea, region)	Landsweiler-Reden, Saarland
[Ref.: #43768]	Country	Germany
[Ref.: #43768]	Country ISO 3 Code	DEU
[Ref.: #43768]	Continent	Europe
[Ref.: #43768]	Isolation date	2007
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Decomposing plant
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GU249609 (>99% sequence identity) for Aetherobacter from Microbeatlas

Aquatic Samples	22
Soil Samples	315
Animal Samples	8
Plant Samples	3
Total Samples	348

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22656]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22656]	Aetherobacter fasciculatus strain SBSr002 16S ribosomal RNA gene, complete sequence	GU249609	1548	GenBank ENA	888830 tax ID

[Ref.: #22656]

GC-content

68.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #22656]

Culture collection no.

DSM 24601, NCCB 100377

[Ref.: #89857]

SI-ID 405449

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Aetherobacter fasciculatus gen. nov., sp. nov. and Aetherobacter rufus sp. nov., novel myxobacteria with promising biotechnological applications.	Garcia R, Stadler M, Gemperlein K, Muller R	Int J Syst Evol Microbiol	10.1099/ijsem.0.000813	2015	*
Enzymology	Metabolic engineering of Pseudomonas putida for production of docosahexaenoic acid based on a myxobacterial PUFA synthase.	Gemperlein K, Zipf G, Bernauer HS, Muller R, Wenzel SC	Metab Eng	10.1016/j.ymben.2015.11.001	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22656

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24601

#43768

Ronald Garcia, Marc Stadler, Katja Gemperlein and Rolf Müller: Aetherobacter fasciculatus gen. nov., sp. nov. and Aetherobacter rufus sp. nov., novel myxobacteria with promising biotechnological applications. IJSEM 66: 928 - 938 2016 ( DOI 10.1099/ijsem.0.000813 , PubMed 26637815 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#89857

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405449.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Aetherobacter fasciculatus in external resources:
SILVA
BRENDA
PANGAEA
GBIF