Strain identifier

BacDive ID: 131429

Type strain: Yes

Species: Aetherobacter fasciculatus

Strain Designation: SBSr002

Strain history: <- M. Stadler, InterMed Discovery GmbH, Dortmund, Germany, SBSr002 <- R. Garcia, Univ. Saarland, Germany

NCBI tax ID(s): 888830 (species)

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General

@ref: 22656

BacDive-ID: 131429

DSM-Number: 24601

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped

description: Aetherobacter fasciculatus SBSr002 is an aerobe, Gram-negative, motile bacterium that was isolated from dried soil with decaying plant material.

NCBI tax id

  • NCBI tax id: 888830
  • Matching level: species

strain history

  • @ref: 22656
  • history: <- M. Stadler, InterMed Discovery GmbH, Dortmund, Germany, SBSr002 <- R. Garcia, Univ. Saarland, Germany

doi: 10.13145/bacdive131429.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Polyangiaceae
  • genus: Aetherobacter
  • species: Aetherobacter fasciculatus
  • full scientific name: Aetherobacter fasciculatus Garcia et al. 2016

@ref: 22656

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Polyangiaceae

genus: Aetherobacter

species: Aetherobacter fasciculatus

full scientific name: Aetherobacter fasciculatus Garcia et al. 2016

strain designation: SBSr002

type strain: yes

Morphology

cell morphology

  • @ref: 43768
  • gram stain: negative
  • cell length: 2.9-6 µm
  • cell width: 1-1.3 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: gliding

colony morphology

@refincubation periodcolony colormedium used
226568-14 days
43768TransparentVY/2 agars

multimedia

  • @ref: 22656
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_24601-1.jpg
  • caption: vegetative cells on medium 67
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43768VY/2 AGARyes
22656M1DG-MEDIUM (DSMZ Medium 1296)yeshttps://mediadive.dsmz.de/medium/1296Name: M1DG-MEDIUM (DSMZ Medium 1296) Composition: Agar 14.0 g/l Casitone 5.0 g/l Glucose 3.5 g/l MgSO4 x 7 H2O 2.0 g/l CaCl2 x 2 H2O 0.5 g/l Distilled water
22656CY-AGAR (DSMZ Medium 67)yeshttps://mediadive.dsmz.de/medium/67Name: CY-AGAR (DSMZ Medium 67) Composition: Agar 15.0 g/l Casitone 3.0 g/l CaCl2 x 2 H2O 1.36 g/l Yeast extract 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
43768positivegrowth22-23
43768nogrowth26
43768nogrowth37
43768positiveoptimum30
22656positivegrowth28

culture pH

@refabilitytypepHPH range
43768positivegrowth5-9alkaliphile
43768positiveoptimum6-8

Physiology and metabolism

oxygen tolerance

  • @ref: 43768
  • oxygen tolerance: aerobe

compound production

  • @ref: 22656
  • compound: aetheramide A and B

halophily

  • @ref: 43768
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-0.1 %(w/v)

observation

  • @ref: 43768
  • observation: Swarm from ring-or halo-like colonies on yeast agar (VY/2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4376862968cellulose-degradation
4376817029chitin-degradation
4376817234glucose+growth
4376817306maltose+growth
4376816634raffinose+growth
4376828017starch+growth
43768tryptone+growth
43768casein+hydrolysis
437684853esculin+hydrolysis
43768skimmed milk+hydrolysis
4376828017starch+hydrolysis
4376853424tween 20+hydrolysis
4376853426tween 80+hydrolysis
4376837166xylan+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistantresistance conc.
437686104kanamycinyesyes
437689215spectinomycinyesyes
4376827902tetracyclineyesyes
4376827701oxytetracyclineyesyes
437683393carbenicillinyesyes
4376828077rifampicinyesyes
4376871321fusidateyesyes
4376829693thiostreptonyesyes
4376828669bacitracinyesyes50 µg/mL
437683542cephalothinyesyes50 µg/mL
4376824753hygromycinyesyes50 µg/mL
437687507neomycinyesyes50 µg/mL
4376859062polymyxinyesyes50 µg/mL
4376817076streptomycinyesyes50 µg/mL
4376845924trimethoprimyesyes50 µg/mL
4376828864tobramycinyesyes50 µg/mL
4376817833gentamicinyesyes50 µg/mL
437682790apramycinyesyes50 µg/mL
4376828971ampicillinyesyes50 µg/mL

enzymes

@refvalueactivityec
43768beta-glucosidase+3.2.1.21
43768alkaline phosphatase+3.1.3.1
43768esterase (C 4)+
43768esterase Lipase (C 8)+
43768leucine arylamidase+3.4.11.1
43768valine arylamidase+
43768cystine arylamidase+3.4.11.3
43768alpha-chymotrypsin+3.4.21.1
43768acid phosphatase+3.1.3.2
43768naphthol-AS-BI-phosphohydrolase+
43768beta-galactosidase+3.2.1.23
43768lipase (C 14)+/-
43768trypsin-3.4.21.4
43768alpha-galactosidase-3.2.1.22
43768beta-glucuronidase-3.2.1.31
43768alpha-glucosidase-3.2.1.20
43768N-acetyl-beta-glucosaminidase-3.2.1.52
43768alpha-mannosidase-3.2.1.24
43768alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
22656dried soil with decaying plant material
43768Dried soil samples from Saarland Centre for BiodocumentationLandsweiler-Reden, SaarlandGermanyDEUEurope2007

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Decomposing plant
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_138874.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_400;96_7021;97_8420;98_10343;99_138874&stattab=map
  • Last taxonomy: Aetherobacter
  • 16S sequence: GU249609
  • Sequence Identity:
  • Total samples: 348
  • soil counts: 315
  • aquatic counts: 22
  • animal counts: 8
  • plant counts: 3

Safety information

risk assessment

  • @ref: 22656
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22656
  • description: Aetherobacter fasciculatus strain SBSr002 16S ribosomal RNA gene, complete sequence
  • accession: GU249609
  • length: 1548
  • database: nuccore
  • NCBI tax ID: 888830

GC content

  • @ref: 22656
  • GC-content: 68.9
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 22656

culture collection no.: DSM 24601, NCCB 100377

straininfo link

  • @ref: 89857
  • straininfo: 405449

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology26617065Metabolic engineering of Pseudomonas putida for production of docosahexaenoic acid based on a myxobacterial PUFA synthase.Gemperlein K, Zipf G, Bernauer HS, Muller R, Wenzel SCMetab Eng10.1016/j.ymben.2015.11.0012015Amide Synthases/genetics/*metabolism, Cloning, Molecular/methods, Docosahexaenoic Acids/*biosynthesis/genetics/isolation & purification, Fatty Acids, Unsaturated/genetics/*metabolism, Metabolic Engineering/*methods, Myxococcales/*enzymology/genetics, Pseudomonas putida/*enzymology/genetics, Recombinant Proteins/metabolismMetabolism
Phylogeny26637815Aetherobacter fasciculatus gen. nov., sp. nov. and Aetherobacter rufus sp. nov., novel myxobacteria with promising biotechnological applications.Garcia R, Stadler M, Gemperlein K, Muller RInt J Syst Evol Microbiol10.1099/ijsem.0.0008132015

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22656Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-24601Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24601)
43768Ronald Garcia, Marc Stadler, Katja Gemperlein and Rolf MüllerAetherobacter fasciculatus gen. nov., sp. nov. and Aetherobacter rufus sp. nov., novel myxobacteria with promising biotechnological applications10.1099/ijsem.0.000813IJSEM 66: 928-938 201626637815
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89857Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405449.1