Thermomonospora cellulosilytica (DSM 45823, CCTCC AA 2012023, YIM 77510)

Name: Thermomonospora cellulosilytica (DSM 45823, CCTCC AA 2012023, YIM 77510)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130740

Type strain

Culture col. no. DSM 45823 CCTCC AA 2012023 YIM 77510 JCM 30326

NCBI tax ID(s) 1411118

Special Collections DSMZ JCM Wink compendium

Links

Thermomonospora cellulosilytica DSM 45823 is a thermophilic prokaryote that builds an aerial mycelium and was isolated from geothermally heated soil.

thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 396656

JCM 30326,DSM 45823

@ref 20215

Last LPSN update 2025-12-16 10:01:04

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Thermomonospora

Species Thermomonospora cellulosilytica

Full scientific name Thermomonospora cellulosilytica (Jiao et al. 2015) Nouioui et al. 2018

Synonyms (1)

Actinomadura cellulosilytica

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125439	positive	100
125439		94.2

Colony morphology

@ref	Colony color	Medium used
69445	Grey beige (1019)	ISP 7
69445	Grey beige (1019)	suter with tyrosine
69445	Colourless	ISP 6
69445	Beige (1001)	ISP 5
69445	Beige (1001)	suter without tyrosine
69445	Light ivory (1015)	ISP 4
69445	Sand yellow (1002)	ISP 2
69445	Sand yellow (1002), ivory (1014)	ISP 3

Multicellular morphology

@ref	Complex name	Complex color	Medium name
69445	Aerial mycelium	Pure white (9010)	ISP 2
69445	Aerial mycelium	Cream (9001)	ISP 3
69445	Aerial mycelium	Oyster white (103)	ISP 4
69445	Aerial mycelium		ISP 5
69445	Aerial mycelium		ISP 6
69445	Aerial mycelium		ISP 7
69445	Aerial mycelium	Traffic white (9016)	suter with tyrosine
69445	Aerial mycelium	Pure white (9010)	suter without tyrosine

Pigmentation

@ref	Production	Color	Name
69445			Melanin
69445		Sand yellow (1002), brown beige (1011), maize yellow (1006)	soluble pigment

Multimedia

@ref:	69445
multimedia content:	DSM_45823_image3.jpeg
multimedia.multimedia content:	DSM_45823_image3.jpeg
caption:	Plates 45°C (553, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

@ref:	69445
multimedia content:	DSM_45823_image4.jpeg
multimedia.multimedia content:	DSM_45823_image4.jpeg
caption:	Plates 45°C (553, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
22032	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
22032	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
22032	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
22032	positive	growth	45	thermophilic
67770	positive	growth	45	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.6

Spore formation

@ref	Spore formation	Confidence
125439		98.1

Halophily

@ref	Salt	Growth	Tested relation	Concentration
69445	NaCl	positive	growth	0-2.5 %

Observation

67770

Observationquinones: MK-9(H₆), MK-9(H₈)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
69445	22599 ChEBI	arabinose	+/-	growth
69445	62968 ChEBI	cellulose	+	growth
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
69445	28757 ChEBI	fructose	+/-	growth
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
69445	17234 ChEBI	glucose	+	growth
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
69445	17268 ChEBI	inositol	-	growth
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
69445	37684 ChEBI	mannose	+/-	growth
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
69445	16634 ChEBI	raffinose	-	growth
69445	26546 ChEBI	rhamnose	+	growth
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
69445	17992 ChEBI	sucrose	+/-	growth
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
69445	18222 ChEBI	xylose	+	growth

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69445	+	+	-	+	-	-	+	-	+	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69445	not determinedn.d.	+	+	+	+/-	+	+	+	+	+	+/-	+	-	-	+/-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22032	geothermally heated soil	Yunnan Province, Tengchong county	China	CHN	Asia
67770	Geothermally heated soil	Tengchong, Yunnan Province, south-west China	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB859254 (>99% sequence identity) for Thermomonospora cellulosilytica from Microbeatlas

Aquatic Samples	31
Soil Samples	371
Animal Samples	19
Plant Samples	18
Total Samples	439

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22032	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1413883v1 assembly for Thermomonospora cellulosilytica DSM 45823	contig	GCA_014138835	1411118.3	2867988698	1411118	76.19

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22032	Actinomadura cellulosilytica gene for 16S ribosomal RNA, partial sequence	AB859254	1538		1411118
67770	Thermomonospora cellulosilytica strain YIM 77510 16S ribosomal RNA gene, partial sequence	KT884887	671		1411118

GC content

@ref	GC-content (mol%)	Method
22032	70.2	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thermomonospora cellulosilytica DSM 45823
NCBI: GCA_014138835

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	98.10	no
125439	motility	BacteriaNetⓘ	no	94.20	no
125439	gram_stain	BacteriaNetⓘ	positive	100.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomadura cellulosilytica strain DSM 45823
PATRIC: 1411118.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.02	no
125438	anaerobic	anaerobicⓘ	no	94.49	no
125438	spore-forming	spore-formingⓘ	yes	80.68	no
125438	aerobic	aerobicⓘ	yes	88.26	no
125438	thermophilic	thermophileⓘ	no	90.81	no
125438	flagellated	motile2+ⓘ	no	87.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Phylogeny	Actinomadura amylolytica sp. nov. and Actinomadura cellulosilytica sp. nov., isolated from geothermally heated soil.	Jiao JY, Liu L, Zhou EM, Wei DQ, Ming H, Xian WD, Yuan CG, Zhong JM, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-015-0465-8	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22032	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45823
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69445	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermomonospora cellulosilytica (DSM 45823, CCTCC AA 2012023, YIM 77510)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014138835

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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