Name: Thermomonospora amylolytica DSM 45822
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130741

Type strain:

Species: Thermomonospora amylolytica

Culture col. no.: DSM 45822, CCTCC AA 2012024, YIM 77502, JCM 30324

Strain history: W.-J. Li; Yunnan Inst. of Microbiol., Yunnan Univ., China; YIM 77502.

NCBI tax ID(s): 1411117 (species)

SI-ID 402203

DSM 45822

Section

Thermomonospora amylolytica DSM 45822 is a spore-forming, thermophilic bacterium that builds an aerial mycelium and was isolated from geothermally heated soil.

spore-forming
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Thermomonospora*
	Species	**Thermomonospora amylolytica**
	Full Scientific Name (LPSN)	Thermomonospora amylolytica (Jiao et al. 2015) Nouioui et al. 2018

Synonym

Actinomadura amylolytica

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %90.818

[Ref.: #69444]	Colony color	Grey beige (1019)
[Ref.: #69444]	Cultivation medium used	ISP 7

[Ref.: #69444]	Colony color	Beige (1001)
[Ref.: #69444]	Cultivation medium used	ISP 2

[Ref.: #69444]	Colony color	Grey beige (1019), stone grey (7030)
[Ref.: #69444]	Cultivation medium used	suter with tyrosine

[Ref.: #69444]	Colony color	Ivory (1014)
[Ref.: #69444]	Cultivation medium used	suter without tyrosine

[Ref.: #69444]	Colony color	Light ivory (1015)
[Ref.: #69444]	Cultivation medium used	ISP 3

[Ref.: #69444]	Colony color	Light ivory (1015)
[Ref.: #69444]	Cultivation medium used	ISP 4

[Ref.: #69444]	Colony color	Light ivory (1015)
[Ref.: #69444]	Cultivation medium used	ISP 5

[Ref.: #69444]	Colony color	Sand yellow (1002)
[Ref.: #69444]	Cultivation medium used	ISP 6

[Ref.: #69444]	Pigment production	yes
[Ref.: #69444]	Pigment name	Melanin
[Ref.: #69444]	Pigment production	yes
[Ref.: #69444]	Pigmentation color	Sand yellow (1002), ocher brown (8001), clay brown (8003) , copper brown (8004)
[Ref.: #69444]	Pigment name	soluble pigment

[Ref.: #69444]	Multicellular complex forming ability	yes
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Multicellular complex color	Traffic white (9016)
[Ref.: #69444]	Medium Name (multicellularity)	ISP 2

[Ref.: #69444]	Multicellular complex forming ability	yes
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Multicellular complex color	Oyster white (1013)
[Ref.: #69444]	Medium Name (multicellularity)	ISP 3

[Ref.: #69444]	Multicellular complex forming ability	no
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Medium Name (multicellularity)	ISP 4

[Ref.: #69444]	Multicellular complex forming ability	no
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Medium Name (multicellularity)	ISP 5

[Ref.: #69444]	Multicellular complex forming ability	no
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Medium Name (multicellularity)	ISP 6

[Ref.: #69444]	Multicellular complex forming ability	no
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Medium Name (multicellularity)	ISP 7

[Ref.: #69444]	Multicellular complex forming ability	no
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69444]	Multicellular complex forming ability	no
[Ref.: #69444]	Multicellular complex name	Aerial mycelium
[Ref.: #69444]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69444]

Caption:

Plates 45°C (553, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69444]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69444]

Caption:

Plates 45°C (553, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69444]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22033]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #22033]

Culture medium growth

[Ref.: #22033]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22033]

Culture medium composition

expand / minimize

[Ref.: #22033]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #22033]

Culture medium growth

[Ref.: #22033]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22033]

Culture medium composition

expand / minimize

[Ref.: #22033]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #22033]

Culture medium growth

[Ref.: #22033]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22033]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #22033]			growth	45 °C
[Ref.: #67770]			growth	45 °C

[Ref.: #22033]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69444]		NaCl		growth	0-2.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₆), MK-9(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69444]	arabinose ChEBI	+	growth
[Ref.: #69444]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69444]	fructose ChEBI	+/-	growth
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69444]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69444]	inositol ChEBI	+/-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69444]	mannose ChEBI	+/-	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69444]	raffinose ChEBI	-	growth
[Ref.: #69444]	rhamnose ChEBI	+	growth
[Ref.: #69444]	sucrose ChEBI	+/-	growth
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69444]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69444]	3937	-	-	-	+	-	-	-	-	+	-	+	not determinedn.d.	+	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69444]	6121	not determinedn.d.	+	+	+	+	+	+	+	+	+	+/-	+/-	-	-	-	-	+/-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22033]	Sample type/isolated from	geothermally heated soil
[Ref.: #22033]	Geographic location (country and/or sea, region)	Yunnan Province, Tengchong county
[Ref.: #22033]	Country	China
[Ref.: #22033]	Country ISO 3 Code	CHN
[Ref.: #22033]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Geothermally heated soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Tengchong, Yunnan Province, south-west China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22033]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22033]	Actinomadura amylolytica gene for 16S ribosomal RNA, partial sequence	AB859253	1540	ENA	1411117 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermomonospora amylolytica YIM 77502	GCA_003589885	complete	GenBank	1411117 tax ID	*
[Ref.: #66792]	Actinomadura amylolytica strain YIM 77502	1411117.3	complete	PATRIC	1411117 tax ID	*
[Ref.: #66792]	Actinomadura amylolytica YIM 77502	2856830813	complete	JGI IMG/M	1411117 tax ID	*

[Ref.: #22033]

GC-content

71.3 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermomonospora amylolytica YIM 77502 NCBI: GCA_003589885.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermomonospora amylolytica YIM 77502 NCBI: GCA_003589885.1
[Ref.: #69480]	motile	no	92.957	no
[Ref.: #69480]	flagellated	no	98.103	no
[Ref.: #69480]	gram-positive	yes	88.946	no
[Ref.: #69480]	anaerobic	no	97.934	no
[Ref.: #69480]	aerobic	yes	84.761	no
[Ref.: #69480]	halophile	no	92.115	no
[Ref.: #69480]	spore-forming	yes	92.793	no
[Ref.: #69480]	glucose-util	yes	87.2	no
[Ref.: #69480]	thermophile	no	82.801	yes
[Ref.: #69480]	glucose-ferment	no	91.144	no

Availability in culture collections External links

[Ref.: #22033]

Culture collection no.

DSM 45822, CCTCC AA 2012024, YIM 77502, JCM 30324

[Ref.: #89241]

SI-ID 402203

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Expression and Characteristics of Two Glucose-Tolerant GH1 beta-glucosidases From Actinomadura amylolytica YIM 77502(T) for Promoting Cellulose Degradation.	Yin YR, Sang P, Xian WD, Li X, Jiao JY, Liu L, Hozzein WN, Xiao M, Li WJ	Front Microbiol	10.3389/fmicb.2018.03149	2018	*
Phylogeny	Actinomadura amylolytica sp. nov. and Actinomadura cellulosilytica sp. nov., isolated from geothermally heated soil.	Jiao JY, Liu L, Zhou EM, Wei DQ, Ming H, Xian WD, Yuan CG, Zhong JM, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-015-0465-8	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22033

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45822

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69444

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#89241

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402203.1 )

* These data were automatically processed and therefore are not curated

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Thermomonospora amylolytica YIM 77502

Thermomonospora amylolytica YIM 77502

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