Thermomonospora amylolytica (DSM 45822, CCTCC AA 2012024, YIM 77502)

Name: Thermomonospora amylolytica (DSM 45822, CCTCC AA 2012024, YIM 77502)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130741

Type strain

Culture col. no. DSM 45822 CCTCC AA 2012024 YIM 77502 JCM 30324

NCBI tax ID(s) 1411117

Special Collections DSMZ JCM Wink compendium

History <- S.-K. Tang, Yunnan Univ., Yunnan Inst. Microbiol. (YIM), Kunming, China; YIM 77502 <- E.-M. Zhou, Yunnan Inst. of Microbiology W.-J. Li; Yunnan Inst. of Microbiol., Yunnan Univ., China; YIM 77502.

Links

Thermomonospora amylolytica DSM 45822 is a bacterium that builds an aerial mycelium and was isolated from geothermally heated soil.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 402203

JCM 30324,DSM 45822

@ref 20215

Last LPSN update 2026-02-09 11:31:17

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Thermomonospora

Species Thermomonospora amylolytica

Full scientific name Thermomonospora amylolytica (Jiao et al. 2015) Nouioui et al. 2018

Synonyms (1)

Actinomadura amylolytica

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125439	positive	99.9
125439		92.1

Colony morphology

@ref	Colony color	Medium used
69444	Grey beige (1019)	ISP 7
69444	Beige (1001)	ISP 2
69444	Grey beige (1019), stone grey (7030)	suter with tyrosine
69444	Ivory (1014)	suter without tyrosine
69444	Light ivory (1015)	ISP 3
69444	Light ivory (1015)	ISP 4
69444	Light ivory (1015)	ISP 5
69444	Sand yellow (1002)	ISP 6

Multicellular morphology

@ref	Complex name	Complex color	Medium name
69444	Aerial mycelium	Traffic white (9016)	ISP 2
69444	Aerial mycelium	Oyster white (1013)	ISP 3
69444	Aerial mycelium		ISP 4
69444	Aerial mycelium		ISP 5
69444	Aerial mycelium		ISP 6
69444	Aerial mycelium		ISP 7
69444	Aerial mycelium		suter with tyrosine
69444	Aerial mycelium		suter without tyrosine

Pigmentation

@ref	Production	Color	Name
69444			melanin pigment
69444		Sand yellow (1002), ocher brown (8001), clay brown (8003) , copper brown (8004)	soluble pigment

Multimedia

@ref:	69444
multimedia content:	DSM_45822_image3.jpeg
multimedia.multimedia content:	DSM_45822_image3.jpeg
caption:	Plates 45°C (553, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

@ref:	69444
multimedia content:	DSM_45822_image4.jpeg
multimedia.multimedia content:	DSM_45822_image4.jpeg
caption:	Plates 45°C (553, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
22033	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
22033	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
22033	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
22033	positive	growth	45
67770	positive	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.4

Spore formation

@ref	Spore formation	Confidence
125439		96.7

Halophily

@ref	Salt	Growth	Tested relation	Concentration
69444	NaCl	positive	growth	0-2.5 %

Observation

67770

Observationquinones: MK-9(H₆), MK-9(H₈)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
69444	22599 ChEBI	arabinose	+	growth
69444	62968 ChEBI	cellulose	-	growth
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
69444	28757 ChEBI	fructose	+/-	growth
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
69444	17234 ChEBI	glucose	+	growth
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
69444	17268 ChEBI	inositol	+/-	growth
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
69444	37684 ChEBI	mannose	+/-	growth
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
69444	16634 ChEBI	raffinose	-	growth
69444	26546 ChEBI	rhamnose	+	growth
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
69444	17992 ChEBI	sucrose	+/-	growth
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
69444	18222 ChEBI	xylose	+	growth

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69444	-	-	-	+	-	-	-	-	+	-	+	not determinedn.d.	+	-	-	-	-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69444	not determinedn.d.	+	+	+	+	+	+	+	+	+	+/-	+/-	-	-	-	-	+/-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22033	geothermally heated soil	Yunnan Province, Tengchong county	China	CHN	Asia
67770	Geothermally heated soil	Tengchong, Yunnan Province, south-west China	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22033	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM358988v1 assembly for Thermomonospora amylolytica YIM 77502	complete	GCA_003589885	1411117.3	2856830813	1411117	96.61

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22033	Actinomadura amylolytica gene for 16S ribosomal RNA, partial sequence	AB859253	1540		1411117
124043	Actinomadura amylolytica strain YIM 77502 16S ribosomal RNA gene, partial sequence.	KT884886	671		1411117

GC content

@ref	GC-content (mol%)	Method
22033	71.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomadura amylolytica strain YIM 77502
PATRIC: 1411117.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	96.70	no
125439	motility	BacteriaNetⓘ	no	92.10	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermomonospora amylolytica YIM 77502
NCBI: GCA_003589885.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.34	no
125438	anaerobic	anaerobicⓘ	no	95.30	no
125438	aerobic	aerobicⓘ	yes	87.76	no
125438	spore-forming	spore-formingⓘ	yes	82.30	no
125438	thermophilic	thermophileⓘ	no	91.67	no
125438	flagellated	motile2+ⓘ	no	89.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Metabolism	Expression and Characteristics of Two Glucose-Tolerant GH1 beta-glucosidases From Actinomadura amylolytica YIM 77502(T) for Promoting Cellulose Degradation.	Yin YR, Sang P, Xian WD, Li X, Jiao JY, Liu L, Hozzein WN, Xiao M, Li WJ	Front Microbiol	10.3389/fmicb.2018.03149	2018
Phylogeny	Actinomadura amylolytica sp. nov. and Actinomadura cellulosilytica sp. nov., isolated from geothermally heated soil.	Jiao JY, Liu L, Zhou EM, Wei DQ, Ming H, Xian WD, Yuan CG, Zhong JM, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-015-0465-8	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22033	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45822
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69444	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermomonospora amylolytica (DSM 45822, CCTCC AA 2012024, YIM 77502)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003589885

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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