Cytobacillus horneckiae (DSM 23495, MTCC 9535, NRRL B-59162)

Name: Cytobacillus horneckiae (DSM 23495, MTCC 9535, NRRL B-59162)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1297

Type strain

Strain Designation 1P01SC

Culture col. no. DSM 23495 MTCC 9535 NRRL B-59162 1P01SC CIP 110062

NCBI tax ID(s) 549687

Special Collections DSMZ Literature strains CIP

History 2009, K. Venkateswaran, Californian Inst. Technol., Pasadena, California, USA: strain 1P01SC <- C. M. Manaia, Escola Superior Biotecnol. (UCP), Porto, Portugal; 1P01SC <- ARS Culture Collection (NRRL) <- P. A. Vaishampayan, California Inst. Technology (Caltech), Pasadena, USA CIP <- 2009, K. Venkateswaran, Californian Inst. Technol., Pasadena, California, USA: strain 1P01SC

Links

Cytobacillus horneckiae 1P01SC is an aerobe, spore-forming, Gram-positive bacterium that was isolated from spacecraft-assembly clean room.

spore-forming Gram-positive motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 370147

DSM 23495,NRRL B-59162,CIP 110062

@ref 20215

Last LPSN update 2026-02-09 11:18:04

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Cytobacillaceae

Genus Cytobacillus

Species Cytobacillus horneckiae

Full scientific name Cytobacillus horneckiae (Vaishampayan et al. 2010) Patel and Gupta 2020

Synonyms (1)

Bacillus horneckiae

BacDive ID	Other strains from **Cytobacillus horneckiae** (1)	Type strain
162240	C. horneckiae JCM 18375

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
29227	positive	5 µm	1.25 µm	rod-shaped
119972	positive			rod-shaped

Pigmentation

29227

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
17238	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled water
37909	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119972	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
29227	positive	growth	04-49
29227	positive	optimum	30
37909	positive	growth	30

Culture pH

@ref	Ability	Type	PH	PH range
29227	positive	optimum	7
29227	positive	growth	7-10.8	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
29227	aerobe
119972	obligate aerobe

Spore formation

@ref	Spore formation	Confidence
29227
125438		91.571

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29227	NaCl	positive	growth	0-10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29227	17128 ChEBI	adipate	+	carbon source
29227	25115 ChEBI	malate	+	carbon source
29227	17306 ChEBI	maltose	+	carbon source
29227	17632 ChEBI	nitrate	+	reduction
119972	17632 ChEBI	nitrate	+	reduction
119972	16301 ChEBI	nitrite	-	reduction
29227	53258 ChEBI	sodium citrate	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119972	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
29227	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
29227	catalase	+	1.11.1.6
119972	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
29227	gelatinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119972	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
119972	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	L-lactaldehyde degradation	100	3 of 3
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	ribulose monophosphate pathway	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	butanoate fermentation	100	4 of 4
66794	sulfopterin metabolism	100	4 of 4
66794	aminopropanol phosphate biosynthesis	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	adipate degradation	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	lipoate biosynthesis	100	5 of 5
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	phenylmercury acetate degradation	100	2 of 2
66794	enterobactin biosynthesis	100	3 of 3
66794	biotin biosynthesis	100	4 of 4
66794	propanol degradation	100	7 of 7
66794	vitamin K metabolism	100	5 of 5
66794	pentose phosphate pathway	100	11 of 11
66794	coenzyme A metabolism	100	4 of 4
66794	isoleucine metabolism	100	8 of 8
66794	aspartate and asparagine metabolism	100	9 of 9
66794	threonine metabolism	100	10 of 10
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	tetrahydrofolate metabolism	100	14 of 14
66794	vitamin B1 metabolism	100	13 of 13
66794	teichoic acid biosynthesis	100	1 of 1
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	aerobactin biosynthesis	100	1 of 1
66794	gluconeogenesis	100	8 of 8
66794	suberin monomers biosynthesis	100	2 of 2
66794	ceramide biosynthesis	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	propionate fermentation	100	10 of 10
66794	alanine metabolism	93.1	27 of 29
66794	proline metabolism	90.91	10 of 11
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	NAD metabolism	88.89	16 of 18
66794	chorismate metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	ketogluconate metabolism	87.5	7 of 8
66794	tryptophan metabolism	86.84	33 of 38
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	citric acid cycle	85.71	12 of 14
66794	leucine metabolism	84.62	11 of 13
66794	phenylalanine metabolism	84.62	11 of 13
66794	vitamin B12 metabolism	82.35	28 of 34
66794	methionine metabolism	80.77	21 of 26
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	3-chlorocatechol degradation	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	Entner Doudoroff pathway	80	8 of 10
66794	pyrimidine metabolism	80	36 of 45
66794	glycine betaine biosynthesis	80	4 of 5
66794	purine metabolism	78.72	74 of 94
66794	heme metabolism	78.57	11 of 14
66794	photosynthesis	78.57	11 of 14
66794	urea cycle	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	cyclohexanol degradation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	vitamin B6 metabolism	72.73	8 of 11
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	lipid metabolism	70.97	22 of 31
66794	arginine metabolism	70.83	17 of 24
66794	phenol degradation	70	14 of 20
66794	histidine metabolism	68.97	20 of 29
66794	non-pathway related	68.42	26 of 38
66794	oxidative phosphorylation	68.13	62 of 91
66794	cyanate degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	acetoin degradation	66.67	2 of 3
66794	degradation of sugar alcohols	62.5	10 of 16
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	starch degradation	60	6 of 10
66794	factor 420 biosynthesis	60	3 of 5
66794	hydrogen production	60	3 of 5
66794	cellulose degradation	60	3 of 5
66794	gallate degradation	60	3 of 5
66794	lysine metabolism	59.52	25 of 42
66794	glutathione metabolism	57.14	8 of 14
66794	tyrosine metabolism	57.14	8 of 14
66794	phenylpropanoid biosynthesis	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	ethanol fermentation	50	1 of 2
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	resorcinol degradation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	selenocysteine biosynthesis	50	3 of 6
66794	androgen and estrogen metabolism	50	8 of 16
66794	glycine metabolism	50	5 of 10
66794	coenzyme M biosynthesis	50	5 of 10
66794	toluene degradation	50	2 of 4
66794	cholesterol biosynthesis	45.45	5 of 11
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	creatinine degradation	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	myo-inositol biosynthesis	40	4 of 10
66794	degradation of pentoses	39.29	11 of 28
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	degradation of sugar acids	36	9 of 25
66794	polyamine pathway	34.78	8 of 23
66794	lipid A biosynthesis	33.33	3 of 9
66794	arachidonic acid metabolism	33.33	6 of 18
66794	degradation of hexoses	33.33	6 of 18
66794	octane oxidation	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	allantoin degradation	33.33	3 of 9
66794	nitrate assimilation	33.33	3 of 9
66794	ascorbate metabolism	31.82	7 of 22
66794	sulfate reduction	30.77	4 of 13
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	metabolism of disaccharids	27.27	3 of 11
66794	d-xylose degradation	27.27	3 of 11
66794	vitamin E metabolism	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	carotenoid biosynthesis	22.73	5 of 22

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119972	-	+	+	+	-	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#Clean room

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
17238	spacecraft-assembly clean room	Kennedy Space Center	USA	USA	North America
119972	Kennedy Space Center, clear room	Florida	United States of America	USA	North America	2007

Taxonmaps

69479

Global distribution of 16S sequence EU861362 (>99% sequence identity) for Cytobacillus horneckiae subclade from Microbeatlas

Aquatic Samples	50
Soil Samples	279
Animal Samples	140
Plant Samples	34
Total Samples	503

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17238	1	Risk group (German classification) According to TRBA 466
119972	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM283573v1 assembly for Cytobacillus horneckiae 1P01SC	contig	GCA_002835735	549687.5	2795385397	549687	56.53

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
17238	Bacillus horneckiae strain 1P01SC 16S ribosomal RNA gene, partial sequence	EU861362	1354	549687
124043	Bacillus horneckiae partial 16S rRNA gene, type strain DSM 23495T	FR749913	1539	549687
124043	Cytobacillus horneckiae strain DSM 23495 16S ribosomal RNA gene, partial sequence.	MW111206	601	549687

GC content

@ref	GC-content (mol%)	Method
17238	35.6	thermal denaturation, midpoint method (Tm)
29227	35.6

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Cytobacillus horneckiae 1P01SC
NCBI: GCA_002835735.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	76.11	yes
125438	anaerobic	anaerobicⓘ	no	92.17	yes
125438	aerobic	aerobicⓘ	yes	70.85	no
125438	spore-forming	spore-formingⓘ	yes	91.57	yes
125438	thermophilic	thermophileⓘ	no	90.34	yes
125438	flagellated	motile2+ⓘ	yes	79.68	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus ciccensis sp. nov., isolated from maize (Zea mays L.) seeds.	Liu Y, Li N, Eom MK, Schumann P, Zhang X, Cao Y, Ge Y, Xiao M, Zhao J, Cheng C, Kim SG	Int J Syst Evol Microbiol	10.1099/ijsem.0.002341	2017
Phylogeny	Bacillus praedii sp. nov., isolated from purplish paddy soil.	Liu B, Liu GH, Sengonca C, Schumann P, Wang JP, Zhu YJ, Liu QY, Wang MK	Int J Syst Evol Microbiol	10.1099/ijsem.0.002030	2017
Phylogeny	Bacillus depressus sp. nov., isolated from soil of a sunflower field.	Wei X, Xin D, Xin Y, Zhang H, Wang T, Zhang J	Antonie Van Leeuwenhoek	10.1007/s10482-015-0605-1	2015
Phylogeny	Bacillus solani sp. nov., isolated from rhizosphere soil of a potato field.	Liu B, Liu GH, Sengonca C, Schumann P, Ge CB, Wang JP, Cui WD, Lin NQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000539	2015
Phylogeny	Bacillus gottheilii sp. nov., isolated from a pharmaceutical manufacturing site.	Seiler H, Wenning M, Schmidt V, Scherer S	Int J Syst Evol Microbiol	10.1099/ijs.0.036277-0	2012
Phylogeny	Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site.	Seiler H, Schmidt V, Wenning M, Scherer S	Int J Syst Evol Microbiol	10.1099/ijs.0.027771-0	2011
Phylogeny	Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant.	Vaz-Moreira I, Figueira V, Lopes AR, Lobo-da-Cunha A, Sproer C, Schumann P, Nunes OC, Manaia CM	Int J Syst Evol Microbiol	10.1099/ijs.0.028605-0	2011
Phylogeny	Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room.	Vaishampayan P, Probst A, Krishnamurthi S, Ghosh S, Osman S, McDowall A, Ruckmani A, Mayilraj S, Venkateswaran K	Int J Syst Evol Microbiol	10.1099/ijs.0.008979-0	2009

References

#17238	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23495
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25648	IJSEM 1031 2010 ( DOI 10.1099/ijs.0.008979-0 , PubMed 19666815 )
#29227	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25648
#37909	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119972	Collection of Institut Pasteur ; Curators of the CIP; CIP 110062
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Cytobacillus horneckiae (DSM 23495, MTCC 9535, NRRL B-59162)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

Pathway annotation

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002835735

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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