Strain identifier
BacDive ID: 1297
Type strain:
Species: Cytobacillus horneckiae
Strain Designation: 1P01SC
Strain history: CIP <- 2009, K. Venkateswaran, Californian Inst. Technol., Pasadena, California, USA: strain 1P01SC
NCBI tax ID(s): 549687 (species)
General
@ref: 17238
BacDive-ID: 1297
DSM-Number: 23495
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped
description: Cytobacillus horneckiae 1P01SC is an aerobe, spore-forming, Gram-positive bacterium that was isolated from spacecraft-assembly clean room.
NCBI tax id
- NCBI tax id: 549687
- Matching level: species
strain history
@ref | history |
---|---|
37909 | 2009, K. Venkateswaran, Californian Inst. Technol., Pasadena, California, USA: strain 1P01SC |
17238 | <- C. M. Manaia, Escola Superior Biotecnol. (UCP), Porto, Portugal; 1P01SC <- ARS Culture Collection (NRRL) <- P. A. Vaishampayan, California Inst. Technology (Caltech), Pasadena, USA |
119972 | CIP <- 2009, K. Venkateswaran, Californian Inst. Technol., Pasadena, California, USA: strain 1P01SC |
doi: 10.13145/bacdive1297.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Cytobacillus
- species: Cytobacillus horneckiae
- full scientific name: Cytobacillus horneckiae (Vaishampayan et al. 2010) Patel and Gupta 2020
synonyms
- @ref: 20215
- synonym: Bacillus horneckiae
@ref: 17238
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Cytobacillus
species: Cytobacillus horneckiae
full scientific name: Cytobacillus horneckiae (Vaishampayan et al. 2010) Patel and Gupta 2020
strain designation: 1P01SC
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
29227 | positive | 5 µm | 1.25 µm | rod-shaped | yes |
119972 | positive | rod-shaped | yes |
pigmentation
- @ref: 29227
- production: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
17238 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled water |
37909 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
119972 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
29227 | positive | growth | 04-49 | |
29227 | positive | optimum | 30 | mesophilic |
37909 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
29227 | positive | growth | 7-10.8 | alkaliphile |
29227 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
29227 | aerobe |
119972 | obligate aerobe |
spore formation
- @ref: 29227
- spore formation: yes
halophily
- @ref: 29227
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-10 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29227 | 17128 | adipate | + | carbon source |
29227 | 25115 | malate | + | carbon source |
29227 | 17306 | maltose | + | carbon source |
29227 | 53258 | sodium citrate | + | carbon source |
29227 | 17632 | nitrate | + | reduction |
119972 | 17632 | nitrate | + | reduction |
119972 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 119972
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
29227 | alkaline phosphatase | + | 3.1.3.1 |
29227 | catalase | + | 1.11.1.6 |
29227 | gelatinase | + | |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
119972 | oxidase | + | |
119972 | catalase | + | 1.11.1.6 |
119972 | urease | + | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119972 | - | + | + | + | - | + | - | - | - | + | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
17238 | spacecraft-assembly clean room | Kennedy Space Center | USA | USA | North America | |
119972 | Kennedy Space Center, clear room | Florida | United States of America | USA | North America | 2007 |
isolation source categories
- Cat1: #Engineered
- Cat2: #Built environment
- Cat3: #Clean room
taxonmaps
- @ref: 69479
- File name: preview.99_542.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_264;97_383;98_440;99_542&stattab=map
- Last taxonomy: Cytobacillus horneckiae subclade
- 16S sequence: EU861362
- Sequence Identity:
- Total samples: 503
- soil counts: 279
- aquatic counts: 50
- animal counts: 140
- plant counts: 34
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
17238 | 1 | Risk group (German classification) |
119972 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 17238
- description: Bacillus horneckiae strain 1P01SC 16S ribosomal RNA gene, partial sequence
- accession: EU861362
- length: 1354
- database: ena
- NCBI tax ID: 549687
Genome sequences
- @ref: 66792
- description: Cytobacillus horneckiae 1P01SC
- accession: GCA_002835735
- assembly level: contig
- database: ncbi
- NCBI tax ID: 549687
GC content
@ref | GC-content | method |
---|---|---|
17238 | 35.6 | thermal denaturation, midpoint method (Tm) |
29227 | 35.6 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | yes | 78.292 | no |
gram-positive | yes | 84.259 | no |
anaerobic | no | 97.7 | yes |
halophile | no | 76.417 | no |
spore-forming | yes | 97.328 | no |
glucose-util | yes | 85.242 | no |
aerobic | yes | 91.826 | yes |
thermophile | no | 97.866 | no |
motile | yes | 84.837 | yes |
glucose-ferment | no | 93.144 | no |
External links
@ref: 17238
culture collection no.: DSM 23495, MTCC 9535, NRRL B-59162, CIP 110062
straininfo link
- @ref: 70948
- straininfo: 370147
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19666815 | Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. | Vaishampayan P, Probst A, Krishnamurthi S, Ghosh S, Osman S, McDowall A, Ruckmani A, Mayilraj S, Venkateswaran K | Int J Syst Evol Microbiol | 10.1099/ijs.0.008979-0 | 2009 | Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Environmental Microbiology, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Spacecraft, Species Specificity | Genetics |
Phylogeny | 21335493 | Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant. | Vaz-Moreira I, Figueira V, Lopes AR, Lobo-da-Cunha A, Sproer C, Schumann P, Nunes OC, Manaia CM | Int J Syst Evol Microbiol | 10.1099/ijs.0.028605-0 | 2011 | Aerobiosis, Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Drinking Water/*microbiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidoreductases/metabolism, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology, Temperature, *Water Purification | Enzymology |
Phylogeny | 21705449 | Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site. | Seiler H, Schmidt V, Wenning M, Scherer S | Int J Syst Evol Microbiol | 10.1099/ijs.0.027771-0 | 2011 | Aerobiosis, Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Chaperonin 60/genetics, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, *Environmental Microbiology, *Food Microbiology, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology, Temperature | Biotechnology |
Phylogeny | 22634699 | Bacillus gottheilii sp. nov., isolated from a pharmaceutical manufacturing site. | Seiler H, Wenning M, Schmidt V, Scherer S | Int J Syst Evol Microbiol | 10.1099/ijs.0.036277-0 | 2012 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Drug Industry, *Environment, Controlled, Fatty Acids/analysis, Germany, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis | Genetics |
Phylogeny | 26289817 | Bacillus solani sp. nov., isolated from rhizosphere soil of a potato field. | Liu B, Liu GH, Sengonca C, Schumann P, Ge CB, Wang JP, Cui WD, Lin NQ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000539 | 2015 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Solanum tuberosum/*microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 26452774 | Bacillus depressus sp. nov., isolated from soil of a sunflower field. | Wei X, Xin D, Xin Y, Zhang H, Wang T, Zhang J | Antonie Van Leeuwenhoek | 10.1007/s10482-015-0605-1 | 2015 | Bacillus/*classification/genetics/growth & development/*isolation & purification, Bacterial Typing Techniques, Base Composition, Benzoquinones/chemistry, Cell Wall/chemistry, China, DNA, Bacterial/analysis/genetics, Diaminopimelic Acid/analysis, Fatty Acids/chemistry, Glycerophospholipids/chemistry, Helianthus/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Sequence Analysis, DNA, *Soil Microbiology | Transcriptome |
Phylogeny | 28820088 | Bacillus praedii sp. nov., isolated from purplish paddy soil. | Liu B, Liu GH, Sengonca C, Schumann P, Wang JP, Zhu YJ, Liu QY, Wang MK | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002030 | 2017 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oryza, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology | Transcriptome |
Phylogeny | 28984568 | Bacillus ciccensis sp. nov., isolated from maize (Zea mays L.) seeds. | Liu Y, Li N, Eom MK, Schumann P, Zhang X, Cao Y, Ge Y, Xiao M, Zhao J, Cheng C, Kim SG | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002341 | 2017 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seeds/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, Zea mays/*microbiology | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
17238 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23495) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23495 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
29227 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25648 | 28776041 | ||
37909 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7877 | |||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
68382 | Automatically annotated from API zym | ||||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
70948 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID370147.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
119972 | Curators of the CIP | Collection of Institut Pasteur (CIP 110062) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110062 |