Name: Azotobacter beijerinckii 3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12725

Type strain:

Species: Azotobacter beijerinckii

Strain Designation: 3, M.B. 2.3.

Culture col. no.: DSM 378, ATCC 19360, NCIB 8948, VKM B-1615, CCUG 53670, LMG 1265, BCRC 14360, CIP 106282, IAM 12683, JCM 20742, CECT 9204, ICMP 8673, NCAIM B.01800, NCIMB 8948, NRRL B-14367, NRRL B-14640

Strain history: CIP <- 1999, NCAIM <- DSMZ <- ATCC <- NCIB <- C.B. van Niel: strain M.B. 2.3. <- S. Soriano: strain 3

NCBI tax ID(s): 170623 (species)

SI-ID 1067

LMG 1265, ATCC 19360, DSM 378, LMG 3850, NCIB 8948, CIP 106282, NCAIM B.01800, VKM B-1615, NCIMB 8948, IAM12683, BCRC 14360, CCRC 14360, ICMP 8673, NRRL B-14367, NRRL B-14640, CCUG 53670, JCM 20742

Other strains from Azotobacter beijerinckii

A. beijerinckii S 16, CCUG 51005, DSM 282
A. beijerinckii 56, DSM 373
A. beijerinckii A 17, DSM 381
A. beijerinckii C-, DSM 1041, NCIB 11292

Section

Azotobacter beijerinckii 3 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge.

Gram-negative
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Azotobacter*
	Species	**Azotobacter beijerinckii**
	Full Scientific Name (LPSN)	Azotobacter beijerinckii Lipman 1904 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #116773]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.989

[Ref.: #116773]	Cell shape	rod-shaped

[Ref.: #116773]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #32950]

Culture medium

MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii

[Ref.: #32950]

Culture medium growth

[Ref.: #32950]

Culture medium composition

expand / minimize

[Ref.: #165]

Culture medium

DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)

[Ref.: #165]

Culture medium growth

[Ref.: #165]

Culture medium link

Medium recipe at MediaDive

[Ref.: #165]

Culture medium composition

expand / minimize

[Ref.: #116773]

Culture medium

CIP Medium 314

[Ref.: #116773]

Culture medium growth

[Ref.: #116773]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #116773]

Culture medium

CIP Medium 1

[Ref.: #116773]

Culture medium growth

[Ref.: #116773]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #165]		growth	30 °C
[Ref.: #32950]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #116773]		growth	25-37 °C
[Ref.: #116773]	no	growth	5 °C
[Ref.: #116773]	no	growth	15 °C
[Ref.: #116773]	no	growth	41 °C
[Ref.: #116773]	no	growth	45 °C

[Ref.: #165]	Temperature range	mesophilic
[Ref.: #32950]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #116773]	Temperature range	mesophilic
[Ref.: #116773]	Temperature range	psychrophilic
[Ref.: #116773]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116773]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.879

	Salt	Tested relation		Salt conc.
[Ref.: #116773]	NaCl		growth	0	%
[Ref.: #116773]	NaCl	no	growth	2	%
[Ref.: #116773]	NaCl	no	growth	4	%
[Ref.: #116773]	NaCl	no	growth	6	%
[Ref.: #116773]	NaCl	no	growth	8	%
[Ref.: #116773]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116773]	esculin ChEBI	+	hydrolysis
[Ref.: #116773]	hippurate ChEBI	-	hydrolysis
[Ref.: #116773]	nitrate ChEBI	-	builds gas from
[Ref.: #116773]	nitrate ChEBI	-	reduction
[Ref.: #116773]	nitrate ChEBI	-	respiration
[Ref.: #116773]	nitrite ChEBI	-	builds gas from
[Ref.: #116773]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #116773]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #116773]		acetoin ChEBI	-
[Ref.: #116773]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #116773]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116773]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116773]	caseinase	-	3.4.21.50
[Ref.: #116773]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #116773]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116773]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #116773]	gelatinase	-
[Ref.: #116773]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #116773]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116773]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116773]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116773]	oxidase	+
[Ref.: #116773]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116773]	tryptophan deaminase	-
[Ref.: #116773]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116773]	9364	-	+	+	+	-	+	+	-	-	-	-	-	+	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #165]	Sample type/isolated from	activated sludge

[Ref.: #59970]	Sample type/isolated from	Sludge,activated

[Ref.: #67770]	Sample type/isolated from	Activated sludge

[Ref.: #116773]	Sample type/isolated from	Environment, Activated sludge
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ308319 (>99% sequence identity) for Azotobacter from Microbeatlas

Aquatic Samples	56
Soil Samples	93
Animal Samples	28
Plant Samples	9
Total Samples	186

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #165]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116773]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Azotobacter beijerinckii partial 16S rRNA gene, isolate ICMP 8673; ATCC 19360	AJ308319	1384	ENA	170623 tax ID	*
[Ref.: #20218]	Azotobacter beijerinckii strain ICMP 8673 16S ribosomal RNA gene, partial sequence	EF100152	1372	ENA	170623 tax ID	*
[Ref.: #20218]	Azotobacter beijerinckii DSM378 16S ribosomal RNA gene, partial sequence	AY336566	863	ENA	170623 tax ID	*
[Ref.: #20218]	Azotobacter beijerinckii strain ICMP 8673 16S ribosomal RNA gene, partial sequence	KC172855	1248	ENA	170623 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Azotobacter beijerinckii DSM 378	GCA_900110885	scaffold	GenBank	170623 tax ID
[Ref.: #66792]	Azotobacter beijerinckii strain DSM 378	170623.6	wgs	PATRIC	170623 tax ID	*
[Ref.: #66792]	Azotobacter beijerinckii DSM 378	2597490340	draft	JGI IMG/M	170623 tax ID	*

[Ref.: #165]

GC-content

66.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Azotobacter beijerinckii DSM 378 NCBI: GCA_900110885.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Azotobacter beijerinckii strain DSM 378 PATRIC: 170623.6
[Ref.: #69480]	motile	no	66.263	no
[Ref.: #69480]	gram-positive	no	98.752	no
[Ref.: #69480]	anaerobic	no	97.979	no
[Ref.: #69480]	aerobic	yes	80.719	no
[Ref.: #69480]	halophile	no	89.41	no
[Ref.: #69480]	spore-forming	no	93.9	no
[Ref.: #69480]	thermophile	no	99.013	no
[Ref.: #69480]	glucose-util	yes	86.788	no
[Ref.: #69480]	flagellated	no	80.859	no
[Ref.: #69480]	glucose-ferment	no	89.644	no

Availability in culture collections External links

[Ref.: #165]

Culture collection no.

DSM 378, ATCC 19360, NCIB 8948, VKM B-1615, CCUG 53670, LMG 1265, BCRC 14360, CIP 106282, IAM 12683, JCM 20742, CECT 9204, ICMP 8673, NCAIM B.01800, NCIMB 8948, NRRL B-14367, NRRL B-14640

[Ref.: #81934]

SI-ID 1067

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.	Fallik E, Chan YK, Robson RL	J Bacteriol	10.1128/jb.173.1.365-371.1991	1991	*

References

#165	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 378

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32950 ; Curators of the CIP;
#59970 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 53670

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81934

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1067.1 )

#116773 Collection of Institut Pasteur ; Curators of the CIP; CIP 106282
* These data were automatically processed and therefore are not curated

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Azotobacter beijerinckii DSM 378

Azotobacter beijerinckii strain DSM 378

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