Name: Hoylesella saccharolytica D033B-12-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12566

Type strain:

Species: Hoylesella saccharolytica

Strain Designation: D033B-12-2

Culture col. no.: DSM 22473, CCUG 57944, JCM 17484

Strain history: CCUG 57944 <-- J. Downes <-- W. E. C. Moore and L. V. Holdeman Moore D033B-12-2.

NCBI tax ID(s): 1236516 (strain), 633701 (species)

SI-ID 400203

DSM 22473, CCUG 57944

Section

Hoylesella saccharolytica D033B-12-2 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from human oral cavity, periodontal pocket in an individual with juvenile periodontitis.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Hoylesella*
	Species	**Hoylesella saccharolytica**
	Full Scientific Name (LPSN)	Hoylesella saccharolytica (Downes et al. 2010) Hitch et al. 2023

Synonym

Prevotella saccharolytica

Information on morphological and physiological properties Morphology

[Ref.: #29517]	Gram stain	negative
[Ref.: #68367]	Gram stain	negative * from API 20A

[Ref.: #29517]	Cell length	0.9-5 µm

[Ref.: #29517]	Cell width	0.6-0.7 µm

[Ref.: #29517]	Cell shape	rod-shaped
[Ref.: #68367]	Cell shape	rod-shaped * from API 20A

[Ref.: #29517]	Motility	no

[Ref.: #61531]

Incubation period

2 days

[Ref.: #29517]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16356]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #16356]

Culture medium growth

[Ref.: #16356]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16356]

Culture medium composition

expand / minimize

[Ref.: #16356]

Culture medium

FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)

[Ref.: #16356]

Culture medium growth

[Ref.: #16356]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16356]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16356]	growth	37 °C
[Ref.: #29517]	growth	25-42 °C
[Ref.: #29517]	optimum	35 °C
[Ref.: #61531]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #16356]	Temperature range	mesophilic
[Ref.: #29517]	Temperature range	mesophilic
[Ref.: #61531]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29517]			optimum	7
[Ref.: #29517]			growth	06-07

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16356]	Oxygen tolerance	anaerobe
[Ref.: #29517]	Oxygen tolerance	anaerobe
[Ref.: #61531]	Oxygen tolerance	anaerobe

[Ref.: #68367]

Ability of spore formation

no * from API 20A

[Ref.: #29517]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29517]	arabinose ChEBI	+	carbon source
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #29517]	cellobiose ChEBI	+	carbon source
[Ref.: #68367]	cellobiose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #29517]	esculin ChEBI	+	hydrolysis
[Ref.: #68367]	esculin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #29517]	fructose ChEBI	+	carbon source
[Ref.: #68367]	gelatin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #29517]	glucose ChEBI	+	carbon source
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68367]	L-rhamnose ChEBI	+	builds acid from	* from API 20A
[Ref.: #29517]	lactose ChEBI	+	carbon source
[Ref.: #68367]	lactose ChEBI	+	builds acid from	* from API 20A
[Ref.: #29517]	maltose ChEBI	+	carbon source
[Ref.: #68367]	maltose ChEBI	+	builds acid from	* from API 20A
[Ref.: #29517]	mannose ChEBI	+	carbon source
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #29517]	raffinose ChEBI	+	carbon source
[Ref.: #68367]	raffinose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #29517]	salicin ChEBI	+	carbon source
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #29517]	sucrose ChEBI	+	carbon source
[Ref.: #68367]	sucrose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	tryptophan ChEBI	-	energy source	* from API 20A, API rID32A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A, API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68367]		indole ChEBI	no	* from API 20A, API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A, API 20A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	+	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68367]	beta-glucosidase	+	3.2.1.21	* from API 20A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68367]	gelatinase	-		* from API 20A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	+		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API 20A
	Only first 10 entries are displayed. Click here to see all.

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #16356]	2059	-	-	+	-	+	+	+	+/-	+/-	+	-	+	-	+	+	-	+	-	+	-	not determinedn.d.	-	-	-

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16356]	12399	-	-	-	+	+/-	+	+/-	+	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16356]	Sample type/isolated from	human oral cavity, periodontal pocket in an individual with juvenile periodontitis
[Ref.: #16356]	Country	USA
[Ref.: #16356]	Country ISO 3 Code	USA
[Ref.: #16356]	Continent	North America

[Ref.: #61531]	Sample type/isolated from	Human oral cavity,peridontal pocket
[Ref.: #61531]	Sampling date	1979-11-01
[Ref.: #61531]	Geographic location (country and/or sea, region)	Virginia
[Ref.: #61531]	Country	USA
[Ref.: #61531]	Country ISO 3 Code	USA
[Ref.: #61531]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Periodontal pocket in an individual with juvenile periodontitis
* marker position based on {}

Isolation sources categories

#Infection	#Inflammation	-
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Periodontal pocket

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ825149 (>99% sequence identity) for Prevotella saccharolytica subclade from Microbeatlas

Aquatic Samples	239
Soil Samples	241
Animal Samples	17642
Plant Samples	74
Total Samples	18196

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16356]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16356]	Prevotella saccharolytica strain D033B-12-2 16S ribosomal RNA gene, partial sequence	FJ825149	1452	ENA	1236516 tax ID
[Ref.: #67770]	Prevotella saccharolytica gene for 16S ribosomal RNA, partial sequence, strain: JCM 17484	AB616135	1491	ENA	1236516 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Hoylesella saccharolytica JCM 17484	GCA_000614205	contig	GenBank	1236516 tax ID
[Ref.: #66792]	Prevotella saccharolytica JCM 17484	1236516.4	wgs	PATRIC	1236516 tax ID	*
[Ref.: #66792]	Prevotella saccharolytica JCM 17484	2585427907	draft	JGI IMG/M	1236516 tax ID	*

[Ref.: #16356]	GC-content	44 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29517]	GC-content	44 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella saccharolytica JCM 17484 PATRIC: 1236516.4
[Ref.: #69480]	motile	no	93.867	yes
[Ref.: #69480]	gram-positive	no	97.801	yes
[Ref.: #69480]	anaerobic	yes	98.452	yes
[Ref.: #69480]	aerobic	no	98.033	yes
[Ref.: #69480]	halophile	no	81.665	no
[Ref.: #69480]	spore-forming	no	97.027	yes
[Ref.: #69480]	glucose-util	yes	82.814	yes
[Ref.: #69480]	flagellated	no	97.686	yes
[Ref.: #69480]	thermophile	no	97.818	yes
[Ref.: #69480]	glucose-ferment	yes	62.282	no

Availability in culture collections External links

[Ref.: #16356]

Culture collection no.

DSM 22473, CCUG 57944, JCM 17484

[Ref.: #81780]

SI-ID 400203

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Prevotella saccharolytica sp. nov., isolated from the human oral cavity.	Downes J, Tanner ACR, Dewhirst FE, Wade WG	Int J Syst Evol Microbiol	10.1099/ijs.0.014720-0	2009	*

References

#16356

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22473

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29517

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25914 (see below)

#61531 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 57944

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68367

Automatically annotated from API 20A .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#81780

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400203.1 )

#25914

IJSEM 2458 2010 ( DOI 10.1099/ijs.0.014720-0 , PubMed 19946051 )

* These data were automatically processed and therefore are not curated

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Prevotella saccharolytica JCM 17484

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