Prevotella buccae (DSM 19025, ATCC 33574, CCUG 15401)

Name: Prevotella buccae (DSM 19025, ATCC 33574, CCUG 15401)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12526

Type strain

Culture col. no. DSM 19025 ATCC 33574 CCUG 15401 CIP 105106 JCM 12245 1 more

NCBI tax ID(s) 873513 28126

Special Collections DSMZ CCUG JCM CIP

History <- CIP <- J.-P. Carlier <- ATCC <- L. V. Holdeman; VPI D3A-6 CCUG 15401 <-- ATCC 33574 <-- L. V. Holdeman VPI D3A-6.

Links

Prevotella buccae DSM 19025 is an anaerobe human pathogen that was isolated from gingival crevice, moderate periodontitis.

anaerobe human pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 45982

ATCC 33574,CCUG 15401,CIP 105106,JCM 12245,DSM 19025

@ref 20215

Last LPSN update 2026-02-09 11:20:51

Domain Bacteria

Phylum Bacteroidota

Class Bacteroidia

Order Bacteroidales

Family Prevotellaceae

Genus Prevotella

Species Prevotella buccae

Full scientific name Prevotella buccae (Holdeman et al. 1982) Shah and Collins 1990

Synonyms (4)

Segatella buccae
Bacteroides capillus
Bacteroides pentosaceus
Bacteroides buccae

BacDive ID	Other strains from **Prevotella buccae** (2)	Type strain
142865	P. buccae CCUG 15403, ATCC 33690
156935	P. buccae CCUG 67165

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	99.2
125438	negative	94.359

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
7845	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
7845	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)		Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
7845	positive	growth	37
46241	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
7845	anaerobe
46241	anaerobe
125438	anaerobe	94.534
125439	anaerobe	99.4

Spore formation

@ref	Spore formation	Confidence
125439		100
125438		92.972

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
68380	alkaline phosphatase	+	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	+	3.2.1.55	from API rID32A
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68380	alpha-galactosidase	+	3.2.1.22	from API rID32A
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	+	3.4.11.1	from API rID32A
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	-		from API rID32A
68380	urease	-	3.5.1.5	from API rID32A

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	glycogen metabolism	100	5 of 5
66794	ppGpp biosynthesis	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	gluconeogenesis	100	8 of 8
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	starch degradation	100	10 of 10
66794	coenzyme A metabolism	100	4 of 4
66794	sulfopterin metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	d-mannose degradation	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	NAD metabolism	83.33	15 of 18
66794	vitamin B6 metabolism	81.82	9 of 11
66794	lipoate biosynthesis	80	4 of 5
66794	vitamin K metabolism	80	4 of 5
66794	threonine metabolism	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	lipid A biosynthesis	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	flavin biosynthesis	73.33	11 of 15
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	pyrimidine metabolism	71.11	32 of 45
66794	glycolysis	70.59	12 of 17
66794	purine metabolism	70.21	66 of 94
66794	Entner Doudoroff pathway	70	7 of 10
66794	phenylalanine metabolism	69.23	9 of 13
66794	octane oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	d-xylose degradation	63.64	7 of 11
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	propionate fermentation	60	6 of 10
66794	methylglyoxal degradation	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	hydrogen production	60	3 of 5
66794	lipid metabolism	58.06	18 of 31
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	glutamate and glutamine metabolism	57.14	16 of 28
66794	citric acid cycle	57.14	8 of 14
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	degradation of hexoses	55.56	10 of 18
66794	pentose phosphate pathway	54.55	6 of 11
66794	methionine metabolism	53.85	14 of 26
66794	leucine metabolism	53.85	7 of 13
66794	histidine metabolism	51.72	15 of 29
66794	alanine metabolism	51.72	15 of 29
66794	ethanol fermentation	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	adipate degradation	50	1 of 2
66794	biotin biosynthesis	50	2 of 4
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	non-pathway related	50	19 of 38
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	phenylmercury acetate degradation	50	1 of 2
66794	degradation of sugar acids	48	12 of 25
66794	lysine metabolism	47.62	20 of 42
66794	degradation of pentoses	46.43	13 of 28
66794	cysteine metabolism	44.44	8 of 18
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	propanol degradation	42.86	3 of 7
66794	tryptophan metabolism	42.11	16 of 38
66794	arginine metabolism	41.67	10 of 24
66794	O-antigen biosynthesis	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	polyamine pathway	39.13	9 of 23
66794	sulfate reduction	38.46	5 of 13
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	oxidative phosphorylation	38.46	35 of 91
66794	ketogluconate metabolism	37.5	3 of 8
66794	metabolism of disaccharids	36.36	4 of 11
66794	proline metabolism	36.36	4 of 11
66794	enterobactin biosynthesis	33.33	1 of 3
66794	L-lactaldehyde degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	nitrate assimilation	33.33	3 of 9
66794	molybdenum cofactor biosynthesis	33.33	3 of 9
66794	ascorbate metabolism	31.82	7 of 22
66794	degradation of sugar alcohols	31.25	5 of 16
66794	urea cycle	30.77	4 of 13
66794	glycine metabolism	30	3 of 10
66794	vitamin B12 metabolism	29.41	10 of 34
66794	arachidonic acid metabolism	27.78	5 of 18
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	glutathione metabolism	21.43	3 of 14

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
7845	-	-	+	+	-	+	+	+	-	-	+	+	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+/-	-
7845	-	-	+	+	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+/-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Inflammation	-
#Host Body-Site	#Oral cavity and airways	#Gingiva

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
7845	gingival crevice, moderate periodontitis	USA	USA	North America
46241	Gingival crevice,moderate periodontitis
67770	Human gingival sulcus

Taxonmaps

69479

Global distribution of 16S sequence AB547675 (>99% sequence identity) for Prevotella buccae subclade from Microbeatlas

Aquatic Samples	875
Soil Samples	397
Animal Samples	42644
Plant Samples	173
Total Samples	44089

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
7845			2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM18494v1 assembly for Segatella buccae ATCC 33574	scaffold	GCA_000184945	873513.3	649989986	873513	65.03

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Prevotella buccae gene for 16S ribosomal RNA, partial sequence, strain: JCM 12245	AB547675	1480	873513
20218	Prevotella buccae genes for 16S rRNA, ITS, 23S rRNA, strain: ATCC 33574	AB547909	863	873513
7845	Prevotella buccae ATCC 33574 16S ribosomal RNA gene, partial sequence	L16477	1473	873513
7845	Segatella buccae ATCC 33574 16S ribosomal RNA, partial sequence	NR_044631	1473	873513

GC content

@ref	GC-content (mol%)	Method
7845	51.0	sequence analysis
67770	50	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Prevotella buccae ATCC 33574
NCBI: GCA_000184945

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	100.00	no
125439	motility	BacteriaNetⓘ	no	72.50	no
125439	gram_stain	BacteriaNetⓘ	negative	99.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Prevotella buccae ATCC 33574
PATRIC: 873513.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	94.36	no
125438	anaerobic	anaerobicⓘ	yes	94.53	yes
125438	aerobic	aerobicⓘ	no	95.44	no
125438	spore-forming	spore-formingⓘ	no	92.97	no
125438	thermophilic	thermophileⓘ	no	94.08	yes
125438	flagellated	motile2+ⓘ	no	88.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Laser-assisted microbial culturomics.	Qu T, Koch L, Mukherjee R, Tu Y, Seidel AL, Puttmann LD, Winkel A, Yang I, Grischke J, Liu D, Wolkers WF, Kittler S, Chichkov B, Stiesch M, Szafranski SP.	Nat Commun	10.1038/s41467-025-66804-7	2025
	Mediastinitis after endobronchial ultrasound with transbronchial needle aspiration resulting in postpneumonectomy empyema.	Robinson NL, Watkins RD, Tapias LF.	JTCVS Tech	10.1016/j.xjtc.2024.03.012	2024
Genetics	Temporal dynamics and species-level complexity of Prevotella spp. in the human gut microbiota: implications for enterotypes and health.	Han N, Peng X, Zhang T, Qiang Y, Li X, Zhang W.	Front Microbiol	10.3389/fmicb.2024.1414000	2024
	The molecular basis of cereal mixed-linkage beta-glucan utilization by the human gut bacterium Segatella copri.	Golisch B, Cordeiro RL, Fraser ASC, Briggs J, Stewart WA, Van Petegem F, Brumer H.	J Biol Chem	10.1016/j.jbc.2024.107625	2024
Pathogenicity	An electron microscope survey of the surface structures and hydrophobicity of oral and non-oral species of the bacterial genus Bacteroides.	Handley PS, Tipler LS	Arch Oral Biol	10.1016/0003-9969(86)90047-6	1986
Phylogeny	Prevotella koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.	Park SN, Lim YK, Shin JH, Jo E, Chang YH, Shin Y, Paek J, Ji S, Kim H, Kook JK	Curr Microbiol	10.1007/s00284-019-01720-w	2019

References

#7845	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19025
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#46241	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 15401
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68380	Automatically annotated from API rID32A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Prevotella buccae (DSM 19025, ATCC 33574, CCUG 15401)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000184945

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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