Name: Metabacillus herbersteinensis D-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1232

Type strain:

Species: Metabacillus herbersteinensis

Strain Designation: D-1,5a

Culture col. no.: DSM 16534, CCM 7228, CIP 108925

Strain history: CIP <- 2005, CCM <- 2004, H.J. Busse, Vienna Univ., Vienna, Austria: strain D-1,5a

NCBI tax ID(s): 283816 (species)

SI-ID 139327

CCM 7228, DSM 16534, LMG 24741, CIP 108925

Special Collections

Section

Metabacillus herbersteinensis D-1 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from medieval wall painting.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Metabacillus*
	Species	**Metabacillus herbersteinensis**
	Full Scientific Name (LPSN)	Metabacillus herbersteinensis (Wieser et al. 2005) Patel and Gupta 2020

Synonym

Bacillus herbersteinensis

Information on morphological and physiological properties Morphology

[Ref.: #31484]	Gram stain	positive
[Ref.: #119880]	Gram stain	positive

[Ref.: #31484]	Cell shape	rod-shaped
[Ref.: #119880]	Cell shape	rod-shaped

[Ref.: #31484]	Motility	yes
[Ref.: #119880]	Motility	no

[Ref.: #31484]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6472]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6472]

Culture medium growth

[Ref.: #6472]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6472]

Culture medium composition

expand / minimize

[Ref.: #38369]

Culture medium

MEDIUM 328- for nutrient agar

[Ref.: #38369]

Culture medium growth

[Ref.: #38369]

Culture medium composition

expand / minimize

[Ref.: #119880]

Culture medium

CIP Medium 328

[Ref.: #119880]

Culture medium growth

[Ref.: #119880]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6472]	growth	30 °C
[Ref.: #31484]	growth	04-28 °C
[Ref.: #31484]	optimum	16 °C
[Ref.: #38369]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #31484]			optimum	9.5
[Ref.: #31484]			growth	07-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31484]	Oxygen tolerance	aerobe
[Ref.: #119880]	Oxygen tolerance	obligate aerobe

[Ref.: #31484]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31484]		NaCl		growth	0-5	%
[Ref.: #31484]		NaCl		optimum	2.5	%

[Ref.: #31484]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31484]	acetate ChEBI	+	carbon source
[Ref.: #31484]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	aspartate ChEBI	+	carbon source
[Ref.: #31484]	cellobiose ChEBI	+	carbon source
[Ref.: #31484]	citrate ChEBI	+	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	gluconate ChEBI	+	carbon source
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	glucosamine ChEBI	+	carbon source
[Ref.: #31484]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	lactate ChEBI	+	carbon source
[Ref.: #31484]	malate ChEBI	+	carbon source
[Ref.: #31484]	maltose ChEBI	+	carbon source
[Ref.: #31484]	mannitol ChEBI	+	carbon source
[Ref.: #31484]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	melibiose ChEBI	+	carbon source
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119880]	nitrate ChEBI	-	reduction
[Ref.: #119880]	nitrite ChEBI	-	reduction
[Ref.: #31484]	ornithine ChEBI	+	carbon source
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	proline ChEBI	+	carbon source
[Ref.: #31484]	pyruvate ChEBI	+	carbon source
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	rhamnose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	ribose ChEBI	+	carbon source
[Ref.: #31484]	salicin ChEBI	+	carbon source
[Ref.: #31484]	sorbitol ChEBI	+	carbon source
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	sucrose ChEBI	+	carbon source
[Ref.: #31484]	trehalose ChEBI	+	carbon source
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31484]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119880]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #119880]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31484]	catalase	+	1.11.1.6
[Ref.: #119880]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31484]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119880]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #119880]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119880]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119880]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #119880]	11425	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+/-	+/-	+/-	-	-	-	-	+/-	+/-	-	+/-	+/-	+/-	-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	-	-	-	-	+/-	+/-	-	-	-	-	-	-	-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119880]	11553	-	+	+	-	-	-	-	-	-	+	-	-	-	+	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6472]	Sample type/isolated from	medieval wall painting
[Ref.: #6472]	Geographic location (country and/or sea, region)	Styria
[Ref.: #6472]	Country	Austria
[Ref.: #6472]	Country ISO 3 Code	AUT
[Ref.: #6472]	Continent	Europe

[Ref.: #119880]	Sample type/isolated from	Medieval wall painting
[Ref.: #119880]	Geographic location (country and/or sea, region)	Styria
[Ref.: #119880]	Country	Austria
[Ref.: #119880]	Country ISO 3 Code	AUT
[Ref.: #119880]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Painting
#Engineered	#Built environment	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ781029 (>99% sequence identity) for Bacillaceae from Microbeatlas

Aquatic Samples	18
Soil Samples	120
Animal Samples	24
Plant Samples	10
Total Samples	172

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6472]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119880]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31484]	Bacillus herbersteinensis 16S rRNA gene, type strain D-1,5a	AJ781029	1464	GenBank ENA	283816 tax ID

[Ref.: #31484]

GC-content

36.55 mol%

Availability in culture collections External links

[Ref.: #6472]

Culture collection no.

DSM 16534, CCM 7228, CIP 108925

[Ref.: #70888]

SI-ID 139327

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin.	Wang HL, Zhang J, Sun L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002164	2017	*
Phylogeny	Bacillus crassostreae sp. nov., isolated from an oyster (Crassostrea hongkongensis).	Chen JH, Tian XR, Ruan Y, Yang LL, He ZQ, Tang SK, Li WJ, Shi H, Chen YG	Int J Syst Evol Microbiol	10.1099/ijs.0.000139	2015	*
Phylogeny	Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake.	Amoozegar MA, Didari M, Bagheri M, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.050120-0	2013	*
Phylogeny	Bacillus halosaccharovorans sp. nov., a moderately halophilic bacterium from a hypersaline lake.	Mehrshad M, Amoozegar MA, Didari M, Bagheri M, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.046961-0	2013	*
Phylogeny	Bacillus galliciensis sp. nov., isolated from faeces of wild seahorses (Hippocampus guttulatus).	Balcazar JL, Pintado J, Planas M	Int J Syst Evol Microbiol	10.1099/ijs.0.011817-0	2009	*
Phylogeny	Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil.	Lee JC, Lee GS, Park DJ, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.65733-0	2008	*
Phylogeny	Bacillus niabensis sp. nov., isolated from cotton-waste composts for mushroom cultivation.	Kwon SW, Lee SY, Kim BY, Weon HY, Kim JB, Go SJ, Lee GB	Int J Syst Evol Microbiol	10.1099/ijs.0.64178-0	2007	*
Phylogeny	Bacillus herbersteinensis sp. nov.	Wieser M, Worliczek H, Kampfer P, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63660-0	2005	*

References

#6472

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16534

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31484

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27782 (see below)

#38369 ; Curators of the CIP;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70888

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID139327.1 )

#119880 Collection of Institut Pasteur ; Curators of the CIP; CIP 108925

#27782

IJSEM 2119 2005 ( DOI 10.1099/ijs.0.63660-0 , PubMed 16166719 )

* These data were automatically processed and therefore are not curated

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