Metabacillus herbersteinensis (DSM 16534, CCM 7228, D-1,5a)

Name: Metabacillus herbersteinensis (DSM 16534, CCM 7228, D-1,5a)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1232

Type strain

Strain Designation D-1,5a

Culture col. no. DSM 16534 CCM 7228 D-1,5a CIP 108925

NCBI tax ID(s) 283816

Special Collections DSMZ Literature strains CIP

History <- H.-J. Busse <- M. Wieser and H.-J. Busse; D-1,5a CIP <- 2005, CCM <- 2004, H.J. Busse, Vienna Univ., Vienna, Austria: strain D-1,5a

Links

Metabacillus herbersteinensis D-1 is an aerobe, spore-forming, mesophilic prokaryote that was isolated from medieval wall painting.

spore-forming Gram-positive motile rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 139327

CCM 7228,DSM 16534,LMG 24741,CIP 108925

@ref 20215

Last LPSN update 2025-12-16 09:47:03

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Metabacillus

Species Metabacillus herbersteinensis

Full scientific name Metabacillus herbersteinensis (Wieser et al. 2005) Patel and Gupta 2020

Synonyms (1)

Bacillus herbersteinensis

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
31484	positive	rod-shaped
119880	positive	rod-shaped

Colony morphology

Pigmentation

31484

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6472	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
38369	MEDIUM 328- for nutrient agar		Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
119880	CIP Medium 328	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6472	positive	growth	30	mesophilic
31484	positive	growth	04-28
31484	positive	optimum	16	psychrophilic
38369	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
31484	positive	optimum	9.5
31484	positive	growth	07-12	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
31484	aerobe
119880	obligate aerobe

Spore formation

31484

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31484	NaCl	positive	growth	0-5 %
31484	NaCl	positive	optimum	2.5 %

Observation

31484

Observationaggregates in clumps

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31484	30089 ChEBI	acetate	+	carbon source
31484	22599 ChEBI	arabinose	+	carbon source
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
31484	35391 ChEBI	aspartate	+	carbon source
31484	17057 ChEBI	cellobiose	+	carbon source
31484	16947 ChEBI	citrate	+	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
31484	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
31484	24265 ChEBI	gluconate	+	carbon source
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
31484	5417 ChEBI	glucosamine	+	carbon source
31484	17234 ChEBI	glucose	+	carbon source
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
31484	24996 ChEBI	lactate	+	carbon source
31484	25115 ChEBI	malate	+	carbon source
31484	17306 ChEBI	maltose	+	carbon source
31484	29864 ChEBI	mannitol	+	carbon source
31484	37684 ChEBI	mannose	+	carbon source
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
31484	28053 ChEBI	melibiose	+	carbon source
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
119880	17632 ChEBI	nitrate	-	reduction
119880	16301 ChEBI	nitrite	-	reduction
31484	18257 ChEBI	ornithine	+	carbon source
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
31484	26271 ChEBI	proline	+	carbon source
31484	15361 ChEBI	pyruvate	+	carbon source
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
31484	26546 ChEBI	rhamnose	+	carbon source
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31484	33942 ChEBI	ribose	+	carbon source
31484	17814 ChEBI	salicin	+	carbon source
31484	30911 ChEBI	sorbitol	+	carbon source
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
31484	17992 ChEBI	sucrose	+	carbon source
31484	27082 ChEBI	trehalose	+	carbon source
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
31484	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119880	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
119880	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31484	catalase	+	1.11.1.6
119880	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31484	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
119880	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
119880	ornithine decarboxylase	-	4.1.1.17
119880	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
119880	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119880	-	+	+	-	-	-	-	-	-	+	-	-	-	+	-	-	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
119880	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+/-	+/-	+/-	-	-	-	-	+/-	+/-	-	+/-	+/-	+/-	-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	-	-	-	-	+/-	+/-	-	-	-	-	-	-	-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Other	#Painting
#Engineered	#Built environment	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6472	medieval wall painting	Styria	Austria	AUT	Europe
119880	Medieval wall painting	Styria	Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ781029 (>99% sequence identity) for Bacillaceae from Microbeatlas

Aquatic Samples	18
Soil Samples	120
Animal Samples	24
Plant Samples	10
Total Samples	172

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6472	1	Risk group (German classification) According to TRBA 466
119880	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4243333v1 assembly for Metabacillus herbersteinensis CCM 7228	scaffold	GCA_042433335	283816	50.37

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
31484	Bacillus herbersteinensis 16S rRNA gene, type strain D-1,5a	AJ781029	1464		283816
124043	Bacillus herbersteinensis strain CCM 7228 16S ribosomal RNA gene, partial sequence.	MT760123	775		283816

GC content

31484

GC-content (mol%)36.55

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin.	Wang HL, Zhang J, Sun L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002164	2017
Phylogeny	Bacillus crassostreae sp. nov., isolated from an oyster (Crassostrea hongkongensis).	Chen JH, Tian XR, Ruan Y, Yang LL, He ZQ, Tang SK, Li WJ, Shi H, Chen YG	Int J Syst Evol Microbiol	10.1099/ijs.0.000139	2015
Phylogeny	Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake.	Amoozegar MA, Didari M, Bagheri M, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.050120-0	2013
Phylogeny	Bacillus halosaccharovorans sp. nov., a moderately halophilic bacterium from a hypersaline lake.	Mehrshad M, Amoozegar MA, Didari M, Bagheri M, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.046961-0	2013
Phylogeny	Bacillus galliciensis sp. nov., isolated from faeces of wild seahorses (Hippocampus guttulatus).	Balcazar JL, Pintado J, Planas M	Int J Syst Evol Microbiol	10.1099/ijs.0.011817-0	2009
Phylogeny	Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil.	Lee JC, Lee GS, Park DJ, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.65733-0	2008
Phylogeny	Bacillus niabensis sp. nov., isolated from cotton-waste composts for mushroom cultivation.	Kwon SW, Lee SY, Kim BY, Weon HY, Kim JB, Go SJ, Lee GB	Int J Syst Evol Microbiol	10.1099/ijs.0.64178-0	2007
Phylogeny	Bacillus herbersteinensis sp. nov.	Wieser M, Worliczek H, Kampfer P, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63660-0	2005

References

#6472	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16534
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27782	IJSEM 2119 2005 ( DOI 10.1099/ijs.0.63660-0 , PubMed 16166719 )
#31484	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27782
#38369	; Curators of the CIP;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119880	Collection of Institut Pasteur ; Curators of the CIP; CIP 108925
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Metabacillus herbersteinensis (DSM 16534, CCM 7228, D-1,5a)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042433335

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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