Strain identifier

BacDive ID: 1232

Type strain: Yes

Species: Metabacillus herbersteinensis

Strain Designation: D-1,5a

Strain history: CIP <- 2005, CCM <- 2004, H.J. Busse, Vienna Univ., Vienna, Austria: strain D-1,5a

NCBI tax ID(s): 283816 (species)

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General

@ref: 6472

BacDive-ID: 1232

DSM-Number: 16534

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Metabacillus herbersteinensis D-1 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from medieval wall painting.

NCBI tax id

  • NCBI tax id: 283816
  • Matching level: species

strain history

@refhistory
6472<- H.-J. Busse <- M. Wieser and H.-J. Busse; D-1,5a
119880CIP <- 2005, CCM <- 2004, H.J. Busse, Vienna Univ., Vienna, Austria: strain D-1,5a

doi: 10.13145/bacdive1232.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Metabacillus
  • species: Metabacillus herbersteinensis
  • full scientific name: Metabacillus herbersteinensis (Wieser et al. 2005) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus herbersteinensis

@ref: 6472

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Metabacillus

species: Metabacillus herbersteinensis

full scientific name: Metabacillus herbersteinensis (Wieser et al. 2005) Patel and Gupta 2020

strain designation: D-1,5a

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
31484positiverod-shapedyes
119880positiverod-shapedno

colony morphology

  • @ref: 119880

pigmentation

  • @ref: 31484
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6472BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
38369MEDIUM 328- for nutrient agaryesDistilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
119880CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328

culture temp

@refgrowthtypetemperature
6472positivegrowth30
31484positivegrowth04-28
31484positiveoptimum16
38369positivegrowth30

culture pH

@refabilitytypepHPH range
31484positivegrowth07-12alkaliphile
31484positiveoptimum9.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31484aerobe
119880obligate aerobe

spore formation

  • @ref: 31484
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
31484NaClpositivegrowth0-5 %
31484NaClpositiveoptimum2.5 %

observation

  • @ref: 31484
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3148430089acetate+carbon source
3148422599arabinose+carbon source
3148435391aspartate+carbon source
3148417057cellobiose+carbon source
3148416947citrate+carbon source
3148428757fructose+carbon source
3148424265gluconate+carbon source
314845417glucosamine+carbon source
3148417234glucose+carbon source
3148424996lactate+carbon source
3148425115malate+carbon source
3148417306maltose+carbon source
3148429864mannitol+carbon source
3148437684mannose+carbon source
3148428053melibiose+carbon source
3148418257ornithine+carbon source
3148426271proline+carbon source
3148415361pyruvate+carbon source
3148426546rhamnose+carbon source
3148433942ribose+carbon source
3148417814salicin+carbon source
3148430911sorbitol+carbon source
3148417992sucrose+carbon source
3148427082trehalose+carbon source
3148418222xylose+carbon source
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837118305arbutin-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11988017632nitrate-reduction
11988016301nitrite-reduction

metabolite production

  • @ref: 119880
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31484catalase+1.11.1.6
31484cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119880oxidase+
119880alcohol dehydrogenase-1.1.1.1
119880catalase+1.11.1.6
119880lysine decarboxylase-4.1.1.18
119880ornithine decarboxylase-4.1.1.17
119880urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119880-++------+---+--+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119880---------+/-+/-+/-+/-----+/-+/--+/-+/-+/--+/-+/-+/-+/-+/-+/-+/-+/-------+/-+/---------+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6472medieval wall paintingStyriaAustriaAUTEurope
119880Medieval wall paintingStyriaAustriaAUTEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Painting
#Engineered#Built environment

taxonmaps

  • @ref: 69479
  • File name: preview.99_21563.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_96;97_103;98_16260;99_21563&stattab=map
  • Last taxonomy: Bacillaceae
  • 16S sequence: AJ781029
  • Sequence Identity:
  • Total samples: 172
  • soil counts: 120
  • aquatic counts: 18
  • animal counts: 24
  • plant counts: 10

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64721Risk group (German classification)
1198801Risk group (French classification)

Sequence information

16S sequences

  • @ref: 31484
  • description: Bacillus herbersteinensis 16S rRNA gene, type strain D-1,5a
  • accession: AJ781029
  • length: 1464
  • database: nuccore
  • NCBI tax ID: 283816

GC content

  • @ref: 31484
  • GC-content: 36.55

External links

@ref: 6472

culture collection no.: DSM 16534, CCM 7228, CIP 108925

straininfo link

  • @ref: 70888
  • straininfo: 139327

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16166719Bacillus herbersteinensis sp. nov.Wieser M, Worliczek H, Kampfer P, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.63660-02005Austria, Bacillus/chemistry/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Humans, Molecular Sequence Data, *Paintings, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityPathogenicity
Phylogeny17684280Bacillus niabensis sp. nov., isolated from cotton-waste composts for mushroom cultivation.Kwon SW, Lee SY, Kim BY, Weon HY, Kim JB, Go SJ, Lee GBInt J Syst Evol Microbiol10.1099/ijs.0.64178-02007Agaricales, Bacillus/genetics/*isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Gossypium/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyGenetics
Phylogeny18984705Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil.Lee JC, Lee GS, Park DJ, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.65733-02008Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Hydrogen-Ion Concentration, Korea, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Silicon Dioxide, *Soil Microbiology, Species SpecificityGenetics
Phylogeny19661512Bacillus galliciensis sp. nov., isolated from faeces of wild seahorses (Hippocampus guttulatus).Balcazar JL, Pintado J, Planas MInt J Syst Evol Microbiol10.1099/ijs.0.011817-02009Animals, Animals, Wild/microbiology, Bacillus/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Ribosomal/analysis/genetics, Fatty Acids/analysis, Feces/*microbiology, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Smegmamorpha/*microbiology, Spain, Species SpecificityGenetics
Phylogeny23291894Bacillus halosaccharovorans sp. nov., a moderately halophilic bacterium from a hypersaline lake.Mehrshad M, Amoozegar MA, Didari M, Bagheri M, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.046961-02013Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Iran, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Sodium Chloride, Vitamin K 2/analysis, *Water MicrobiologyGenetics
Phylogeny23504967Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake.Amoozegar MA, Didari M, Bagheri M, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.050120-02013Bacillus/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Iran, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, Vitamin K 2/analogs & derivatives/analysis, Water MicrobiologyGenetics
Phylogeny25713049Bacillus crassostreae sp. nov., isolated from an oyster (Crassostrea hongkongensis).Chen JH, Tian XR, Ruan Y, Yang LL, He ZQ, Tang SK, Li WJ, Shi H, Chen YGInt J Syst Evol Microbiol10.1099/ijs.0.0001392015Animals, Bacillus/*classification/genetics/isolation & purification, Base Composition, China, Crassostrea/*microbiology, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny28866991Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin.Wang HL, Zhang J, Sun LInt J Syst Evol Microbiol10.1099/ijsem.0.0021642017Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Nucleic Acid Hybridization, Pacific Ocean, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6472Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16534)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16534
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31484Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2778228776041
38369Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6609
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70888Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID139327.1StrainInfo: A central database for resolving microbial strain identifiers
119880Curators of the CIPCollection of Institut Pasteur (CIP 108925)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108925