Ectobacillus funiculus (DSM 15141, CIP 107128, JCM 11201)

Name: Ectobacillus funiculus (DSM 15141, CIP 107128, JCM 11201)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1210

Type strain

Strain Designation NAF001

Culture col. no. DSM 15141 CIP 107128 JCM 11201 NAF001 KCTC 3796

NCBI tax ID(s) 137993

Special Collections DSMZ JCM KCTC CIP Literature strains

History DSMZ <- R. Iriye CIP <- 2001, R. Iriye, Shinshu University, Japan: strain NAF001 JCM <-- R. Iriye NAF001. CIP <- 2001, R. Iriye, Shinshu Univ., Nagano, Japan: strain NAF001

Links

Ectobacillus funiculus NAF001 is an aerobe, spore-forming, Gram-variable bacterium that forms circular colonies and was isolated from activated sludge of domestic wastewater treatment tanks.

spore-forming Gram-variable motile filament-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 100518

CIP 107128,JCM 11201,DSM 15141,KCTC 3796

@ref 20215

Last LPSN update 2026-05-29 08:25:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Ectobacillus

Species Ectobacillus funiculus

Full scientific name Ectobacillus funiculus (Ajithkumar et al. 2002) Gupta et al. 2020

Synonyms (1)

Bacillus funiculus

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
23310	variable	4.0-6.0 µm	0.8-2.0 µm	filament-shaped		peritrichous
119493	positive			rod-shaped

Colony morphology

@ref	Colony color	Colony shape	Medium used	Hemolysis ability
23310	opaque, off-white	circular	NG agar
119493				0

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5634	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23310	NG agar
37106	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119493	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5634	positive	growth	30
23310	positive	growth	20-40
23310	negative	growth	10
37106	positive	growth	30
67770	positive	growth	30

Culture pH

@ref	Ability	Type	PH
119493	positive	growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23310	aerobe
23310	anaerobe
119493	obligate aerobe
125439	obligate aerobe	93.911

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
23310	centrally located, ellipsoidal	endospore
125439				90.396

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23310	NaCl		growth	5 %
119493	NaCl	positive	growth	0 %
119493	NaCl		growth	2 %
119493	NaCl		growth	4 %
119493	NaCl		growth	6 %
119493	NaCl		growth	8 %
119493	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
23310		casein	-	hydrolysis
23310	16947 ChEBI	citrate	-	assimilation
119493	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
23310	27605 ChEBI	D-psicose	+	assimilation
23310	16988 ChEBI	D-ribose	+	assimilation
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
23310	16443 ChEBI	D-tagatose	-	assimilation
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
23310	16551 ChEBI	D-trehalose	+	assimilation
23310	65327 ChEBI	D-xylose	-	assimilation
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
23310	4853 ChEBI	esculin	+	hydrolysis
119493	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
23310	28757 ChEBI	fructose	+	assimilation
23310	5291 ChEBI	gelatin	-	hydrolysis
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
23310	17234 ChEBI	glucose	+	assimilation
23310	17754 ChEBI	glycerol	+	assimilation
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	+	builds acid from	from API 50CH acid
23310	30849 ChEBI	L-arabinose	-	assimilation
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
23310	15589 ChEBI	L-malate	-	assimilation
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
23310	17716 ChEBI	lactose	-	assimilation
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
23310	17306 ChEBI	maltose	+	assimilation
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
23310	17268 ChEBI	myo-inositol	-	assimilation
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
23310	17632 ChEBI	nitrate	+	reduction
119493	17632 ChEBI	nitrate	+	reduction
119493	17632 ChEBI	nitrate	-	respiration
119493	16301 ChEBI	nitrite	+	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
23310	16634 ChEBI	raffinose	-	assimilation
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
23310	17814 ChEBI	salicin	-	assimilation
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
23310	28017 ChEBI	starch	+	hydrolysis
23310	17992 ChEBI	sucrose	+	assimilation
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
23310	27897 ChEBI	tryptophan	-	energy source
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
23310	53426 ChEBI	tween 80	-	hydrolysis
23310	16199 ChEBI	urea	+	hydrolysis
23310	17151 ChEBI	xylitol	-	assimilation
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
23310	15688 ChEBI	acetoin
23310	35581 ChEBI	indole
119493	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
23310	15688 ChEBI	acetoin	+
119493	15688 ChEBI	acetoin	-
119493	17234 ChEBI	glucose		-
23310	35581 ChEBI	indole			-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
119493	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119493	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
119493	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119493	caseinase	-	3.4.21.50
23310	catalase	+	1.11.1.6
119493	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
23310	cytochrome oxidase	-	1.9.3.1
119493	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119493	gelatinase	-
119493	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
119493	lipase	-
68382	lipase (C 14)	-		from API zym
119493	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119493	ornithine decarboxylase	-	4.1.1.17
119493	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
23310	tryptophan deaminase	-	4.1.99.1
119493	tween esterase	-
119493	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119493	-	-	+	+	-	+	-	-	-	-	-	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
119493	not determinedn.d.	-	+/-	-	+/-	-	+	-	-	-	+/-	+	+	+/-	-	+/-	+/-	+/-	+/-	+	-	-	+	+/-	+	+	+	+/-	+	-	+	+	+	+	+	+	+/-	+	-	+	+	-	-	-	-	+/-	-	+/-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119493	not determinedn.d.	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	-	+	-	+	+	+	-	+	+	-	-	-	+	-	-	+	+	+	+	+	-	+	-	+	-	+	+	+	+	+	-	+	+	-	+	-	+	+	+	-	-	-	+	-	-	-	-	+	-	-	-	+	+	-	+	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#Container (Reservoir)
#Engineered	#Waste	#Activated sludge
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5634	activated sludge of domestic wastewater treatment tanks	Fukushima	Japan	JPN	Asia
67770	Suspended water of a domestic wastewater treatment tank	Nakajima, Nishishirakawa, Fukushima	Japan	JPN	Asia
119493	Environment, Activated sludge of wastewater tanks	Fukushima	Japan	JPN	Asia	1997

Taxonmaps

69479

Global distribution of 16S sequence AB049195 (>99% sequence identity) for Bacillus funiculus subclade from Microbeatlas

Aquatic Samples	1267
Soil Samples	6181
Animal Samples	1450
Plant Samples	1811
Total Samples	10709

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5634	1	Risk group (German classification) According to TRBA 466
119493	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4242943v1 assembly for Ectobacillus funiculus JCM 11201	scaffold	GCA_042429435	137993	27.38
124043	ASM4266108v1 assembly for Ectobacillus funiculus JCM 11201	contig	GCA_042661085	137993	5.3
124043	ASM3953604v1 assembly for Ectobacillus funiculus JCM 11201	contig	GCA_039536045	137993	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Bacillus sp. NAF001 gene for 16S rRNA, partial sequence	AB049195	1530		137993

GC content

@ref	GC-content (mol%)	Method
23310	37.2	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4242943v1 assembly for Ectobacillus funiculus JCM 11201
NCBI: GCA_042429435

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	93.91	no
125439	gram_stain	BacteriaNetⓘ	variable	71.48	no
125439	motility	BacteriaNetⓘ	yes	84.44	no
125439	spore_formation	BacteriaNetⓘ	yes	90.40	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus polymachus sp. nov., with a broad range of antibacterial activity, isolated from forest topsoil samples by using a modified culture method.	Nguyen TM, Kim J	Int J Syst Evol Microbiol	10.1099/ijs.0.070326-0	2014
Phylogeny	Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field.	Ten LN, Baek SH, Im WT, Liu QM, Aslam Z, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.64403-0	2006
Phylogeny	Bacillus funiculus sp. nov., novel filamentous isolates from activated sludge.	Ajithkumar VP, Ajithkumar B, Iriye R, Sakai T	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1141	2002

References

#5634	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15141
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23310	Vasudevan P Ajithkumar, Bindu Ajithkumar, Ryozo Iriye, Tadashi Sakai: Bacillus funiculus sp. nov., novel filamentous isolates from activated sludge.. IJSEM 52: 1141 - 1144 2002 ( DOI 10.1099/00207713-52-4-1141 , PubMed 12148618 )
#37106	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119493	Collection of Institut Pasteur ; Curators of the CIP; CIP 107128
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Ectobacillus funiculus (DSM 15141, CIP 107128, JCM 11201)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042429435

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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