Fictibacillus barbaricus (DSM 14730, CCM 4982, V2-BIII-A2)

Name: Fictibacillus barbaricus (DSM 14730, CCM 4982, V2-BIII-A2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1205

Type strain

Strain Designation V2-BIII-A2, V₂-BIII-A₂

Culture col. no. DSM 14730 CCM 4982 V2-BIII-A2 V₂-BIII-A₂ CIP 108015

NCBI tax ID(s) 182136

Special Collections DSMZ CIP

History <- H.-J. Busse; V₂-BIII-A₂ <- M. Täubel CIP <- 2003, CCM <- H.J. Busse <- M. Taubel, Bacillus barbaricus

Links

Fictibacillus barbaricus V2-BIII-A2 is an obligate aerobe, spore-forming, Gram-positive bacterium that was isolated from grounding of an experimental wall painting.

spore-forming Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 100516

CCM 4982,DSM 14730,CIP 108015,KCTC 3853,LMG 23067

@ref 20215

Last LPSN update 2026-02-09 11:18:03

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Fictibacillus

Species Fictibacillus barbaricus

Full scientific name Fictibacillus barbaricus (Täubel et al. 2003) Glaeser et al. 2013

Synonyms (1)

Bacillus barbaricus

BacDive ID	Other strains from **Fictibacillus barbaricus** (5)	Type strain
1206	F. barbaricus FR1-162con, DSM 30726
153009	F. barbaricus CCUG 48260
154362	F. barbaricus CCUG 53241
154689	F. barbaricus CCUG 54857
157211	F. barbaricus CCUG 70593

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116732	positive	rod-shaped

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5515	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
34951	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116732	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5515	positive	growth	22
34951	positive	growth	30
116732	positive	growth	22-37
116732	negative	growth	10
116732	negative	growth	45
116732	negative	growth	55

Culture pH

@ref	Ability	Type	PH
116732	positive	growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
116732	obligate aerobe
125439	obligate aerobe	94.6

Spore formation

@ref	Spore formation	Confidence
116732
125438		92.391

Halophily

@ref	Salt	Growth	Tested relation	Concentration
116732	NaCl	positive	growth	0 %
116732	NaCl		growth	2 %
116732	NaCl		growth	4 %
116732	NaCl		growth	6 %
116732	NaCl		growth	8 %
116732	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
116732	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
116732	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
116732	17632 ChEBI	nitrate	-	reduction
116732	17632 ChEBI	nitrate	-	respiration
116732	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
116732	45285 ChEBI	pyrazinamide	-	hydrolysis
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116732	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
116732	15688 ChEBI	acetoin	-
116732	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
116732	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
116732	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
116732	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116732	caseinase	+	3.4.21.50
116732	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
116732	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
116732	gamma-glutamyltransferase	-	2.3.2.2
116732	gelatinase	+
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
116732	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116732	ornithine decarboxylase	-	4.1.1.17
116732	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
116732	tween esterase	+
116732	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116732	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
116732	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	+	+/-	+	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	+	-	-	-	+/-	-	-	-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
116732	not determinedn.d.	+	+	+	+	+	-	-	+	-	+	+	-	-	-	-	-	-	+	-	+	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	+	-	+	-	-	-	+	-	+	+	+	+	+	+	+	-	-	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	-
#Engineered	#Other	#Painting

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5515	grounding of an experimental wall painting	Vienna	Austria	AUT	Europe
116732	Sample from a new experimental wall painting		Austria	AUT	Europe	2000

Taxonmaps

69479

Global distribution of 16S sequence AJ422145 (>99% sequence identity) for Fictibacillus from Microbeatlas

Aquatic Samples	774
Soil Samples	2517
Animal Samples	546
Plant Samples	344
Total Samples	4181

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5515	1	Risk group (German classification) According to TRBA 466
116732	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1463502v1 assembly for Fictibacillus barbaricus CCM 4982	contig	GCA_014635025	182136.3	2995388355	182136	74.41
66792	ASM1705255v1 assembly for Fictibacillus barbaricus DSM 14730	contig	GCA_017052555	182136.4	8075116569	182136	69.47

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5515	Bacillus barbaricus partial 16S rRNA gene, strain VII-B3-A2	AJ422145	1421		182136
124043	Fictibacillus barbaricus strain CCM 4982 16S ribosomal RNA gene, partial sequence.	MT760117	1374		182136

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Fictibacillus barbaricus CCM 4982
NCBI: GCA_014635025

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	88.20	no
125439	motility	BacteriaNetⓘ	yes	83.90	no
125439	gram_stain	BacteriaNetⓘ	variable	79.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Fictibacillus barbaricus strain CCM 4982
PATRIC: 182136.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	76.41	no
125438	anaerobic	anaerobicⓘ	no	95.40	no
125438	spore-forming	spore-formingⓘ	yes	92.39	no
125438	aerobic	aerobicⓘ	yes	79.57	yes
125438	thermophilic	thermophileⓘ	no	88.90	no
125438	flagellated	motile2+ⓘ	yes	81.54	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Fictibacillus iocasae sp. nov., isolated from the deep-sea sediment in Pacmanus, Manus Basin.	Wang HL, Zhang J, Sun L	Arch Microbiol	10.1007/s00203-018-1527-x	2018
Phylogeny	Fictibacillus aquaticus sp. nov., isolated from downstream river water.	Pal D, Bhardwaj A, Kaur N, Sudan SK, Bisht B, Kumari M, Vyas B, Krishnamurthi S, Mayilraj S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002474	2017
Phylogeny	Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range.	Sharma A, Kohli P, Singh Y, Schumann P, Lal R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001051	2016
Phylogeny	Fictibacillus enclensis sp. nov., isolated from marine sediment.	Dastager SG, Mawlankar R, Srinivasan K, Tang SK, Lee JC, Ramana VV, Shouche YS	Antonie Van Leeuwenhoek	10.1007/s10482-013-0097-9	2013
Phylogeny	Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus.	Glaeser SP, Dott W, Busse HJ, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.049171-0	2013
Phylogeny	Bacillus tianmuensis sp. nov., isolated from soil in Tianmu Mountain national natural reserve, Hangzhou, China.	Wen YP, Wu XC, Qian CD, Zhao YH, Fang HH, Li O	FEMS Microbiol Lett	10.1111/j.1574-6968.2009.01610.x	2009
Phylogeny	Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India.	Shivaji S, Suresh K, Chaturvedi P, Dube S, Sengupta S	Int J Syst Evol Microbiol	10.1099/ijs.0.63476-0	2005
Phylogeny	Bacillus barbaricus sp. nov., isolated from an experimental wall painting.	Taubel M, Kampfer P, Buczolits S, Lubitz W, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02304-0	2003

References

#5515	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14730
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34951	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116732	Collection of Institut Pasteur ; Curators of the CIP; CIP 108015
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Fictibacillus barbaricus (DSM 14730, CCM 4982, V2-BIII-A2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014635025

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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