Strain identifier

BacDive ID: 1205

Type strain: Yes

Species: Fictibacillus barbaricus

Strain Designation: V2-BIII-A2, V2-BIII-A2

Strain history: CIP <- 2003, CCM <- H.J. Busse <- M. Taubel, Bacillus barbaricus

NCBI tax ID(s): 182136 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5515

BacDive-ID: 1205

DSM-Number: 14730

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, psychrophilic, motile

description: Fictibacillus barbaricus V2-BIII-A2 is an obligate aerobe, spore-forming, psychrophilic bacterium that was isolated from grounding of an experimental wall painting.

NCBI tax id

  • NCBI tax id: 182136
  • Matching level: species

strain history

@refhistory
5515<- H.-J. Busse; V2-BIII-A2 <- M. Täubel
116732CIP <- 2003, CCM <- H.J. Busse <- M. Taubel, Bacillus barbaricus

doi: 10.13145/bacdive1205.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Fictibacillus
  • species: Fictibacillus barbaricus
  • full scientific name: Fictibacillus barbaricus (Täubel et al. 2003) Glaeser et al. 2013

  • synonyms

    • @ref: 20215
    • synonym: Bacillus barbaricus

@ref: 5515

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Fictibacillus

species: Fictibacillus barbaricus

full scientific name: Fictibacillus barbaricus (Täubel et al. 2003) Glaeser et al. 2013

strain designation: V2-BIII-A2, V2-BIII-A2

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.379
69480100positive
116732nopositiverod-shaped

colony morphology

  • @ref: 116732

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5515NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
34951MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116732CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5515positivegrowth22psychrophilic
34951positivegrowth30mesophilic
116732positivegrowth22-37
116732nogrowth10psychrophilic
116732nogrowth45thermophilic
116732nogrowth55thermophilic

culture pH

  • @ref: 116732
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 116732
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100
116732yes

halophily

@refsaltgrowthtested relationconcentration
116732NaClpositivegrowth0 %
116732NaClnogrowth2 %
116732NaClnogrowth4 %
116732NaClnogrowth6 %
116732NaClnogrowth8 %
116732NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116732citrate-carbon source16947
116732hippurate+hydrolysis606565
116732nitrate-reduction17632
116732nitrite-reduction16301
116732pyrazinamide-hydrolysis45285
116732nitrate-respiration17632

metabolite production

  • @ref: 116732
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11673215688acetoin-
11673217234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
116732oxidase-
116732beta-galactosidase-3.2.1.23
116732alcohol dehydrogenase-1.1.1.1
116732gelatinase+
116732amylase+
116732DNase+
116732caseinase+3.4.21.50
116732catalase+1.11.1.6
116732tween esterase+
116732gamma-glutamyltransferase-2.3.2.2
116732lysine decarboxylase-4.1.1.18
116732ornithine decarboxylase-4.1.1.17
116732urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116732--++-------+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116732----+/------++/-+--------+/------+---+/----+/-+/-------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116732+++++--+-++------+-+--+--+-----+---+-----+-------+---------+++-----------+-----++-+---+-+++++++--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5515grounding of an experimental wall paintingViennaAustriaAUTEurope
116732Sample from a new experimental wall paintingAustriaAUTEurope2000

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment
#Engineered#Other#Painting

taxonmaps

  • @ref: 69479
  • File name: preview.99_4364.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1870;97_2247;98_2758;99_4364&stattab=map
  • Last taxonomy: Fictibacillus
  • 16S sequence: AJ422145
  • Sequence Identity:
  • Total samples: 4181
  • soil counts: 2517
  • aquatic counts: 774
  • animal counts: 546
  • plant counts: 344

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
55151Risk group (German classification)
1167321Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5515
  • description: Bacillus barbaricus partial 16S rRNA gene, strain VII-B3-A2
  • accession: AJ422145
  • length: 1421
  • database: ena
  • NCBI tax ID: 182136

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fictibacillus barbaricus CCM 4982GCA_014635025contigncbi182136
66792Fictibacillus barbaricus DSM 14730GCA_017052555contigncbi182136
66792Fictibacillus barbaricus strain CCM 4982182136.3wgspatric182136
66792Fictibacillus barbaricus strain DSM 14730182136.4wgspatric182136

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes88.335no
gram-positiveyes90.885no
anaerobicno98.401no
aerobicyes89.289no
halophileno81.467no
spore-formingyes94.999no
glucose-utilyes90.4no
flagellatedyes76.781no
thermophileno96.492yes
glucose-fermentno91.979no

External links

@ref: 5515

culture collection no.: DSM 14730, CCM 4982, CIP 108015

straininfo link

  • @ref: 70861
  • straininfo: 100516

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12807193Bacillus barbaricus sp. nov., isolated from an experimental wall painting.Taubel M, Kampfer P, Buczolits S, Lubitz W, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.02304-02003Austria, Bacillus/chemistry/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, DNA Fingerprinting, DNA, Ribosomal/analysis, Molecular Sequence Data, *Paintings, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny15879243Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India.Shivaji S, Suresh K, Chaturvedi P, Dube S, Sengupta SInt J Syst Evol Microbiol10.1099/ijs.0.63476-02005Anti-Bacterial Agents/*pharmacology, Arsenic/*pharmacology, Arsenites/pharmacology, Bacillus/*classification/*drug effects/isolation & purification/physiology, Bacterial Typing Techniques, Carbonates, Cyanoacrylates/analysis/isolation & purification, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Diaminopimelic Acid/analysis/isolation & purification, Drug Resistance, Bacterial, Fatty Acids/analysis/isolation & purification, Ferric Compounds, Genes, rRNA, India, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial, Vitamin K 2/analysis/isolation & purificationEnzymology
Phylogeny19459975Bacillus tianmuensis sp. nov., isolated from soil in Tianmu Mountain national natural reserve, Hangzhou, China.Wen YP, Wu XC, Qian CD, Zhao YH, Fang HH, Li OFEMS Microbiol Lett10.1111/j.1574-6968.2009.01610.x2009Bacillus/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny23355698Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus.Glaeser SP, Dott W, Busse HJ, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.049171-02013Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, Polyamines/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisGenetics
Phylogeny24343101Fictibacillus enclensis sp. nov., isolated from marine sediment.Dastager SG, Mawlankar R, Srinivasan K, Tang SK, Lee JC, Ramana VV, Shouche YSAntonie Van Leeuwenhoek10.1007/s10482-013-0097-92013Bacillaceae/chemistry/*classification/genetics/isolation & purification, Geologic Sediments/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *SeawaterGenetics
Phylogeny27031366Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range.Sharma A, Kohli P, Singh Y, Schumann P, Lal RInt J Syst Evol Microbiol10.1099/ijsem.0.0010512016Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA Gyrase/genetics, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny29116040Fictibacillus aquaticus sp. nov., isolated from downstream river water.Pal D, Bhardwaj A, Kaur N, Sudan SK, Bisht B, Kumari M, Vyas B, Krishnamurthi S, Mayilraj SInt J Syst Evol Microbiol10.1099/ijsem.0.0024742017Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny29802421Fictibacillus iocasae sp. nov., isolated from the deep-sea sediment in Pacmanus, Manus Basin.Wang HL, Zhang J, Sun LArch Microbiol10.1007/s00203-018-1527-x2018Bacillaceae/classification/genetics/*isolation & purification/metabolism, Base Composition, Cell Wall/metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/*microbiology, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/classification/genetics/metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitle
5515Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14730)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14730
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34951Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5591
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70861Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100516.1StrainInfo: A central database for resolving microbial strain identifiers
116732Curators of the CIPCollection of Institut Pasteur (CIP 108015)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108015