Sporosarcina pasteurii (DSM 33, ATCC 11859, CCM 2056)

Name: Sporosarcina pasteurii (DSM 33, ATCC 11859, CCM 2056)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11981

Type strain

Strain Designation 22, Gibson 22

Culture col. no. DSM 33 ATCC 11859 CCM 2056 NCIB 8841 NCTC 4822 7 more

NCBI tax ID(s) 1474

Special Collections DSMZ CCUG CIP

History <- ATCC <- F.F. Lombard <- T. Gibson, 22 CIP <- 1966, ATCC <- F.F. Lombard <- T. Gibson: strain 22

Links

Sporosarcina pasteurii 22 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from soil.

Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 2909

LMG 7130,ATCC 11859,CCM 2056,CCUG 7425,DSM 33,NCFB 1776,NCIB 8219,NCIB 8841,NCTC 4822,NCDO 1776,NCIMB 8841,KCTC3558,VKM B-513,CIP 66.21,LMD 48.21,NCIMB8219,NCCB 48021,BCRC 11596,CCRC 11596,CCT 0538,NCTC 48722,NCIB 9241,HAMBI 1877,NRRL NRS-673,NCIM 2477

@ref 20215

Last LPSN update 2026-02-09 11:20:43

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Caryophanaceae

Genus Sporosarcina

Species Sporosarcina pasteurii

Full scientific name Sporosarcina pasteurii (Miquel 1889) Yoon et al. 2001

Synonyms (2)

Bacillus pasteurii
"Urobacillus pasteurii"

BacDive ID	Other strains from **Sporosarcina pasteurii** (6)	Type strain
11979	S. pasteurii 1, DSM 276
11980	S. pasteurii 3, DSM 323
134425	S. pasteurii 2711, CIP 54.16
136414	S. pasteurii 2710, CIP 54.15, NCDO 1168
145737	S. pasteurii CCUG 29455, NRRL NRS-674
145738	S. pasteurii CCUG 29456, NRRL NRS-675

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121627	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2034	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
41674	MEDIUM 19 - for Bacillus pasteurii		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Urea solution - M0090 (100.000 ml)
121627	CIP Medium 19	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
2034	positive	growth	30
41674	positive	growth	30
44994	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
44994	aerobe
121627	obligate aerobe
125439	obligate aerobe	92.2

Spore formation

@ref	Spore formation	Confidence
125439		91

Compound production

2034

Compoundurease

Murein

@ref	Murein short key	Type
2034	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
121627	17632 ChEBI	nitrate	+	reduction
121627	16301 ChEBI	nitrite	-	reduction
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121627	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121627	catalase	+	1.11.1.6
68379	catalase	-	1.11.1.6	from API Coryne
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
121627	oxidase	+
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
121627	urease	+	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	adipate degradation	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	propanol degradation	100	7 of 7
66794	gluconeogenesis	100	8 of 8
66794	palmitate biosynthesis	100	22 of 22
66794	valine metabolism	100	9 of 9
66794	biotin biosynthesis	100	4 of 4
66794	sulfopterin metabolism	100	4 of 4
66794	lipoate biosynthesis	100	5 of 5
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	teichoic acid biosynthesis	100	1 of 1
66794	vitamin B1 metabolism	92.31	12 of 13
66794	threonine metabolism	90	9 of 10
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	leucine metabolism	84.62	11 of 13
66794	hydrogen production	80	4 of 5
66794	flavin biosynthesis	80	12 of 15
66794	peptidoglycan biosynthesis	80	12 of 15
66794	vitamin K metabolism	80	4 of 5
66794	citric acid cycle	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	alanine metabolism	75.86	22 of 29
66794	ketogluconate metabolism	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	purine metabolism	74.47	70 of 94
66794	tryptophan metabolism	73.68	28 of 38
66794	pyrimidine metabolism	73.33	33 of 45
66794	methionine metabolism	73.08	19 of 26
66794	proline metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	photosynthesis	71.43	10 of 14
66794	glycolysis	70.59	12 of 17
66794	starch degradation	70	7 of 10
66794	propionate fermentation	70	7 of 10
66794	urea cycle	69.23	9 of 13
66794	degradation of sugar alcohols	68.75	11 of 16
66794	glutamate and glutamine metabolism	67.86	19 of 28
66794	cyanate degradation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	lysine metabolism	64.29	27 of 42
66794	oxidative phosphorylation	63.74	58 of 91
66794	pentose phosphate pathway	63.64	7 of 11
66794	androgen and estrogen metabolism	62.5	10 of 16
66794	cysteine metabolism	61.11	11 of 18
66794	gallate degradation	60	3 of 5
66794	3-chlorocatechol degradation	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	histidine metabolism	58.62	17 of 29
66794	non-pathway related	57.89	22 of 38
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	d-mannose degradation	55.56	5 of 9
66794	phenol degradation	55	11 of 20
66794	arginine metabolism	54.17	13 of 24
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	lipid metabolism	51.61	16 of 31
66794	toluene degradation	50	2 of 4
66794	kanosamine biosynthesis II	50	1 of 2
66794	Entner Doudoroff pathway	50	5 of 10
66794	ribulose monophosphate pathway	50	1 of 2
66794	heme metabolism	50	7 of 14
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	tyrosine metabolism	50	7 of 14
66794	glycine metabolism	50	5 of 10
66794	mevalonate metabolism	42.86	3 of 7
66794	glutathione metabolism	42.86	6 of 14
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	arachidonate biosynthesis	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	vitamin B6 metabolism	36.36	4 of 11
66794	ascorbate metabolism	36.36	8 of 22
66794	degradation of pentoses	35.71	10 of 28
66794	IAA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	phosphatidylethanolamine bioynthesis	30.77	4 of 13
66794	myo-inositol biosynthesis	30	3 of 10
66794	coenzyme M biosynthesis	30	3 of 10
66794	vitamin B12 metabolism	29.41	10 of 34
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	degradation of hexoses	27.78	5 of 18
66794	metabolism of disaccharids	27.27	3 of 11
66794	d-xylose degradation	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	CMP-KDO biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	cyclohexanol degradation	25	1 of 4
66794	degradation of sugar acids	24	6 of 25
66794	sulfate reduction	23.08	3 of 13
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	nitrate assimilation	22.22	2 of 9
66794	lipid A biosynthesis	22.22	2 of 9
66794	allantoin degradation	22.22	2 of 9
66794	4-hydroxymandelate degradation	22.22	2 of 9

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
44994	+	-	+	-	-	-	+	-	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121627	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
2034	soil
121627	Environment, Soil

Taxonmaps

69479

Global distribution of 16S sequence HQ676600 (>99% sequence identity) for Sporosarcina from Microbeatlas

Aquatic Samples	421
Soil Samples	1270
Animal Samples	2467
Plant Samples	192
Total Samples	4350

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2034	1	Risk group (German classification) According to TRBA 466
121627	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Sporosarcina pasteurii ATCC 11859	complete			2582581329	1474	98.58
124043	ASM4129557v1 assembly for Sporosarcina pasteurii DSM 33	complete	GCA_041295575			1474	80.43
66792	53694_E01 assembly for Sporosarcina pasteurii NCTC4822	contig	GCA_900457495	1474.8	2849954708	1474	72.19
124043	ASM3182239v1 assembly for Sporosarcina pasteurii DSM 33	contig	GCA_031822395	1474.20		1474	58.6

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Sporosarcina pasteurii NCCB:48021 16S ribosomal RNA gene, partial sequence	HQ676600	1476		1474
20218	B.pasteurii 16S ribosomal RNA	X60631	1431		1474

GC content

@ref	GC-content (mol%)	Method
2034	38.5	thermal denaturation, midpoint method (Tm)
2034	38.4	Buoyant density centrifugation (BD)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Sporosarcina pasteurii NCTC4822
NCBI: GCA_900457495

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	91.00	no
125439	motility	BacteriaNetⓘ	yes	86.90	no
125439	gram_stain	BacteriaNetⓘ	variable	81.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	92.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Sporosarcina pasteurii strain NCTC4822
PATRIC: 1474.8

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	72.76	no
125438	anaerobic	anaerobicⓘ	no	91.69	yes
125438	spore-forming	spore-formingⓘ	yes	81.06	no
125438	aerobic	aerobicⓘ	yes	68.74	no
125438	thermophilic	thermophileⓘ	no	87.10	no
125438	flagellated	motile2+ⓘ	yes	77.49	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Application of Sporosarcina pasteurii for the biomineralization of calcite in the treatment of waste concrete fines.	Klikova K, Holecek P, Nezerka V, Prosek Z, Konakova D, Demnerova K, Stiborova H.	Environ Sci Pollut Res Int	10.1007/s11356-025-36102-2	2025
Genetics	Genome-guided development of a bacterial two-strain system for low-temperature soil biocementation.	Ciuchcinski K, Czerwonka G, Decewicz P, Godlewska Z, Misiolek K, Zegadlo K, Styczynski M, Dziewit L.	Appl Microbiol Biotechnol	10.1007/s00253-025-13448-8	2025
	The Effect of Bacteria-to-Calcium Ratio on Microbial-Induced Carbonate Precipitation (MICP) under Different Sequences of Calcium-Source Introduction.	Zhao T, Du H, Shang R.	Materials (Basel)	10.3390/ma17081881	2024
	Mobility, speciation of cadmium, and bacterial community composition along soil depths during microbial carbonate precipitation under simulated acid rain.	Li W, Cai Y, Li Y, Achal V.	J Environ Manage	10.1016/j.jenvman.2024.120018	2024
	Influencing factors on ureolytic microbiologically induced calcium carbonate precipitation for biocementation.	Erdmann N, Strieth D.	World J Microbiol Biotechnol	10.1007/s11274-022-03499-8	2022
	Bio-Stimulated Surface Healing of Historical and Compatible Conservation Mortars.	Vucetic S, Cjepa D, Miljevic B, Bergh JMV, Sovljanski O, Tomic A, Nikolic E, Markov S, Hirsenberger H, Ranogajec J.	Materials (Basel)	10.3390/ma16020642	2023
	Living Porous Ceramics for Bacteria-Regulated Gas Sensing and Carbon Capture.	Dutto A, Kan A, Saraw Z, Maillard A, Zindel D, Studart AR.	Adv Mater	10.1002/adma.202412555	2025
Biotechnology	Microbially Induced Calcium Carbonate Precipitation by Sporosarcina pasteurii: a Case Study in Optimizing Biological CaCO₃ Precipitation.	Carter MS, Tuttle MJ, Mancini JA, Martineau R, Hung CS, Gupta MK.	Appl Environ Microbiol	10.1128/aem.01794-22	2023
Biotechnology	Surface-displayed silicatein-alpha enzyme in bioengineered E. coli enables biocementation and silica mineralization	Vigil T, Schwendeman N, Grogger M, Morrison V, Warner M, Bone N, Vance M, Morris D, McElmurry K, Berger B, Steel J.	Front Syst Biol		2024
	Biologically-induced synthetic manganese carbonate precipitate (BISMCP) for potential applications in heavy metal removal.	Dewi AK, Sharma RK, Das K, Sukul U, Lin PY, Huang YH, Lu CM, Lu CK, Chen TH, Chen CY.	Heliyon	10.1016/j.heliyon.2023.e15919	2023
	New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings.	Vincent J, Colin B, Lanneluc I, Sabot R, Sopena V, Turcry P, Mahieux PY, Refait P, Jeannin M, Sable S.	Microorganisms	10.3390/microorganisms10010076	2021
	Experimental Study on Bio-Reinforcement of Calcareous Sand through Hydrochloric Acid Solution Precipitation into Cementing Solution.	Jiang Z, Wei R, Dai D, Li L, Shang Z, Tang J, Peng J, Li P.	Materials (Basel)	10.3390/ma16196348	2023
	Impact of treatment methods on recycled concrete aggregate performance: a comprehensive review.	Peiris D, Gunasekara C, Law DW, Patrisia Y, Tam VWY, Setunge S.	Environ Sci Pollut Res Int	10.1007/s11356-025-36497-y	2025
	Reduction of bioavailability and phytotoxicity effect of cadmium in soil by microbial-induced carbonate precipitation using metabolites of ureolytic bacterium Ochrobactrum sp. POC9.	Zakrzewska M, Rzepa G, Musialowski M, Goszcz A, Stasiuk R, Debiec-Andrzejewska K.	Front Plant Sci	10.3389/fpls.2023.1109467	2023
	Microbiologically Induced Carbonate Precipitation in the Restoration and Conservation of Cultural Heritage Materials.	Ortega-Villamagua E, Gudino-Gomezjurado M, Palma-Cando A.	Molecules	10.3390/molecules25235499	2020
Metabolism	Modeling the Effect of Microbially Induced Calcium Carbonate Precipitation (MICP) on CO₂ Trapping.	Chen R, Kavala AM, Clara Saracho A, Marek EJ.	Environ Sci Technol	10.1021/acs.est.5c08890	2025
	Inhibition of Urease by Hydroquinones: A Structural and Kinetic Study.	Mazzei L, Cianci M, Ciurli S.	Chemistry	10.1002/chem.202201770	2022
	Microbial functionalities and immobilization of environmental lead: Biogeochemical and molecular mechanisms and implications for bioremediation.	Elizabeth George S, Wan Y.	J Hazard Mater	10.1016/j.jhazmat.2023.131738	2023
Metabolism	Genetic optimisation of bacteria-induced calcite precipitation in Bacillus subtilis.	Hoffmann TD, Paine K, Gebhard S.	Microb Cell Fact	10.1186/s12934-021-01704-1	2021
	Assessment of ecofriendly carbon capture using Bacillus subtilis induced calcium carbonate precipitation with focus on applications mechanisms and cost efficiency.	Danial AW, Hasan RMM, Mahmoud GA, Abdel-Basset R.	Sci Rep	10.1038/s41598-025-06688-1	2025
Metabolism	In-Depth Profiling of Calcite Precipitation by Environmental Bacteria Reveals Fundamental Mechanistic Differences with Relevance to Application.	Reeksting BJ, Hoffmann TD, Tan L, Paine K, Gebhard S.	Appl Environ Microbiol	10.1128/aem.02739-19	2020
	Relationship between Bacterial Contribution and Self-Healing Effect of Cement-Based Materials.	Sovljanski O, Tomic A, Markov S.	Microorganisms	10.3390/microorganisms10071399	2022
Biotechnology	Complete genome sequence of Sporosarcina pasteurii type strain DSM33.	Tuttle MJ, Stamps BW, Chodkowski JL, Mechan-Llontop ME, Shade A, Hung C-S, Gupta MK, Carter MS.	Microbiol Resour Announc	10.1128/mra.00843-24	2024
Genetics	Adaptive Evolution of Sporosarcina pasteurii Enhances Saline-Alkali Resistance for High-Performance Concrete Crack Repair via MICP.	Liu J, Xu H, Dong M, Cheng Z, Mi C, Sun S, Zhu R, Han P.	Microorganisms	10.3390/microorganisms13071526	2025
	Enhancing microbial-induced calcium carbonate precipitation efficiency in calcareous sands through ferric ion additives: A comprehensive experimental investigation.	Zhu J, Wei R, Dai D, Li L, Shang Z, Jiang Z, Peng J.	PLoS One	10.1371/journal.pone.0327568	2025
	Experimental study on alkali reduction of film-coated porous ecological concrete by microbial calcium carbonate precipitation technology.	Wang B, Yang B, Wang L, Lian B, Wu X.	Sci Rep	10.1038/s41598-023-50637-9	2024
	Evaluation and optimal width ratio selection of microbial mineralization technique in the repair of lining cracks in Xinjiang desert open channel.	Wang J, Zhu J, Li Y, Zhang S, Feng C.	Sci Rep	10.1038/s41598-025-01582-2	2025
	Cyanobacterial Biocrust on Biomineralized Soil Mitigates Freeze-Thaw Effects and Preserves Structure and Ecological Functions.	Kimura K, Okuro T.	Microb Ecol	10.1007/s00248-024-02389-w	2024
	Experimental Study on Silt Soil Improved by Microbial Solidification with the Use of Lignin.	Sun Y, Zhong X, Lv J, Wang G.	Microorganisms	10.3390/microorganisms11020281	2023
	Effect of cell density on decrease in hydraulic conductivity by microbial calcite precipitation.	Eryuruk K.	AMB Express	10.1186/s13568-022-01448-0	2022
	Phenotypic Characterization and Draft Genome Sequence Analyses of Two Novel Endospore-Forming Sporosarcina spp. Isolated from Canada Goose (Branta canadensis) Feces.	Keshri J, Smith KM, Svendsen MK, Keillor HR, Moss ML, Jordan HJ, Larkin AM, Garrish JK, Line JE, Ball PN, Oakley BB, Seal BS.	Microorganisms	10.3390/microorganisms12010070	2023
	Effect of substrate mineralogy, biofilm and extracellular polymeric substances on bacterially induced carbonate mineralisation investigated with in situ nanoscale ToF-SIMS	Dubey A, Toprak P, Pring A, Rodriguez-Navarro C, Mukherjee A, Dhami N.	Sci Rep		2025
	Bacteria-powered self-healing concrete: Breakthroughs, challenges, and future prospects.	Elgendy IM, Elkaliny NE, Saleh HM, Darwish GO, Almostafa MM, Metwally K, Yahya G, Mahmoud YA.	J Ind Microbiol Biotechnol	10.1093/jimb/kuae051	2024
	Mechanical properties and microstructural analysis of MICP-reinforced coarse-grained saline soils under freeze-thaw cycling.	Xiong L, Tian L, Zhang X, Wang M, Ahemaiti A.	PLoS One	10.1371/journal.pone.0336266	2025
	Experimental Study on Different Improvement Schemes of EICP-Lignin Solidified Silt.	Sun Y, Zhong X, Lv J, Wang G, Hu R.	Materials (Basel)	10.3390/ma16030999	2023
Biotechnology	Surface-displayed silicatein-alpha enzyme in bioengineered E. coli enables biocementation and silica mineralization	Vigil T, Schwendeman N, Grogger M, Morrison V, Warner M, Bone N, Vance M, Morris D, McElmurry K, Berger B, Steel J.	Front Syst Biol		2024
	Synergistic Effect of Microbial-Induced Carbonate Precipitation Modified with Hydroxypropyl Methylcellulose on Improving Loess Disintegration and Seepage Resistance.	Wang X, Sun H.	Polymers (Basel)	10.3390/polym17040548	2025
	Laboratory Study of Dynamic Durability and Material Properties of Bio-Cemented Sand for Green Road Base Applications.	Ainiwaer F, Hou T, Huang R, Li J, Fan L, Bao W.	Materials (Basel)	10.3390/ma18174178	2025
	Biomineralization of coral sand by Bacillus thuringiensis isolated from a travertine cave.	Xiao Y, Deng H, Li J.	Sci Rep	10.1038/s41598-023-35893-z	2023
	Microbial Carbonation of Monocalcium Silicate.	Guzman MS, Iyer J, Kim P, Kopp D, Dong Z, Foroughi P, Yung MC, Riman RE, Jiao Y.	ACS Omega	10.1021/acsomega.1c05264	2022
	Whey as an Alternative Nutrient Medium for Growth of Sporosarcina pasteurii and Its Effect on CaCO₃ Polymorphism and Fly Ash Bioconsolidation.	Chaparro S, Rojas HA, Caicedo G, Romanelli G, Pineda A, Luque R, Martinez JJ.	Materials (Basel)	10.3390/ma14102470	2021
	Precipitation of Magnetic Iron Oxide Induced by Sporosarcina pasteurii Cells.	Wu Y, Zhao G, Qi H.	Microorganisms	10.3390/microorganisms9020331	2021
Metabolism	Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii.	Murugan R, Suraishkumar GK, Mukherjee A, Dhami NK.	Sci Rep	10.1038/s41598-021-00315-5	2021
	Controlling the calcium carbonate microstructure of engineered living building materials.	Clara Saracho A, Lucherini L, Hirsch M, Peter HM, Terzis D, Amstad E, Laloui L.	J Mater Chem A Mater	10.1039/d1ta03990c	2021
	Optimization of culture medium to improve bio-cementation effect based on response surface method.	Pan Z, Cao S.	Sci Rep	10.1038/s41598-024-58063-1	2024
	Experimental Study on the Wind Erosion Resistance of Aeolian Sand Solidified by Microbially Induced Calcite Precipitation (MICP).	Qu J, Li G, Ma B, Liu J, Zhang J, Liu X, Zhang Y.	Materials (Basel)	10.3390/ma17061270	2024
	Crack Sealing in Concrete with Biogrout: Sustainable Approach to Enhancing Mechanical Strength and Water Resistance.	Wang J, Ji S, Huang S, Jiang Z, Wang S, Zhang H, Wang Z, Zhang J.	Materials (Basel)	10.3390/ma17246283	2024
	Microfluidic study in a meter-long reactive path reveals how the medium's structural heterogeneity shapes MICP-induced biocementation.	Elmaloglou A, Terzis D, De Anna P, Laloui L.	Sci Rep	10.1038/s41598-022-24124-6	2022
	Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments.	Lapierre FM, Bolz I, Buchs J, Huber R.	Front Bioeng Biotechnol	10.3389/fbioe.2022.936759	2022
Metabolism	Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface.	Ghosh T, Bhaduri S, Montemagno C, Kumar A.	PLoS One	10.1371/journal.pone.0210339	2019
	Effects of Air Entrainment on Bacterial Viability in Cement Paste.	Kim H, Son HM.	Materials (Basel)	10.3390/ma15062163	2022
	Effect of Jute Fibres on the Process of MICP and Properties of Biocemented Sand.	Spencer CA, van Paassen L, Sass H.	Materials (Basel)	10.3390/ma13235429	2020
	Shear Strength Behaviors of Aeolian Sand Solidified by Microbially Induced Calcite Precipitation and Basalt Fiber Reinforcement.	Li G, Liu J, Zhang J, Yang Y, Chen S.	Materials (Basel)	10.3390/ma16175857	2023
	Bacteria Engineered to Produce Serotonin Modulate Host Intestinal Physiology.	Mavros CF, Bongers M, Neergaard FBF, Cusimano F, Sun Y, Kaufman A, Richardson M, Kammler S, Kristensen M, Sommer MOA, Wang HH.	ACS Synth Biol	10.1021/acssynbio.4c00453	2024
	Use of Bacteria to Activate Ground-Granulated Blast-Furnace Slag (GGBFS) as Cementless Binder.	Yum WS, Do J.	Materials (Basel)	10.3390/ma15103620	2022
	Experimental Study on the Mechanical Behaviors of Aeolian Sand Treated by Microbially Induced Calcite Precipitation (MICP) and Basalt Fiber Reinforcement (BFR).	Liu J, Li X, Li G, Zhang J.	Materials (Basel)	10.3390/ma16051949	2023
	Formations of calcium carbonate minerals by bacteria and its multiple applications.	Anbu P, Kang CH, Shin YJ, So JS.	Springerplus	10.1186/s40064-016-1869-2	2016
	Biosynthesis of Ag3 PO4 nanoparticles in the absence of phosphate source using a phosphorus mineralising bacterium	Rahimi M, Hosseini M, Bakhshi M.	IET Nanobiotechnol		2017
Genetics	Spatiotemporal dynamics during niche remodeling by super-colonizing microbiota in the mammalian gut.	Urtecho G, Moody T, Huang Y, Sheth RU, Richardson M, Descamps HC, Kaufman A, Lekan O, Zhang Z, Velez-Cortes F, Qu Y, Cohen L, Ricaurte D, Gibson TE, Gerber GK, Thaiss CA, Wang HH.	Cell Syst	10.1016/j.cels.2024.10.007	2024
	Bacterial Community Dynamics and Biocement Formation during Stimulation and Augmentation: Implications for Soil Consolidation.	Dhami NK, Alsubhi WR, Watkin E, Mukherjee A.	Front Microbiol	10.3389/fmicb.2017.01267	2017
	Fabrication of patterned calcium carbonate materials through template-assisted microbially induced calcium carbonate precipitation.	Yi D, Zhang H, Zhang W, Zong Y, Zhao K.	RSC Adv	10.1039/d1ra04072c	2021
	Mineralogy, morphology, and reaction kinetics of ureolytic bio-cementation in the presence of seawater ions and varying soil materials.	Burdalski RJ, Ribeiro BGO, Gomez MG, Gorman-Lewis D.	Sci Rep	10.1038/s41598-022-21268-3	2022
Metabolism	Influence of temperature on microbially induced calcium carbonate precipitation for soil treatment.	Peng J, Liu Z.	PLoS One	10.1371/journal.pone.0218396	2019
Enzymology	Effects of Calcium Source on Biochemical Properties of Microbial CaCO3 Precipitation.	Xu J, Du Y, Jiang Z, She A.	Front Microbiol	10.3389/fmicb.2015.01366	2015
	Insights into the influence of cell concentration in design and development of microbially induced calcium carbonate precipitation (MICP) process.	Murugan R, Suraishkumar GK, Mukherjee A, Dhami NK.	PLoS One	10.1371/journal.pone.0254536	2021
	Incorporation of Mixing Microbial Induced Calcite Precipitation (MICP) with Pretreatment Procedure for Road Soil Subgrade Stabilization.	Hu X, Fu X, Pan P, Lin L, Sun Y.	Materials (Basel)	10.3390/ma15196529	2022
	Characterisation of CaCO₃ phases during strain-specific ureolytic precipitation.	Clara Saracho A, Haigh SK, Hata T, Soga K, Farsang S, Redfern SAT, Marek E.	Sci Rep	10.1038/s41598-020-66831-y	2020
	Investigating the potential for microbially induced carbonate precipitation to treat mine waste.	Proudfoot D, Brooks L, Gammons CH, Barth E, Bless D, Nagisetty RM, Lauchnor EG.	J Hazard Mater	10.1016/j.jhazmat.2021.127490	2022
	Tuning Polymorphs and Morphology of Microbially Induced Calcium Carbonate: Controlling Factors and Underlying Mechanisms.	Khanjani M, Westenberg DJ, Kumar A, Ma H.	ACS Omega	10.1021/acsomega.1c00559	2021
	Ureolytic/Non-Ureolytic Bacteria Co-Cultured Self-Healing Agent for Cementitious Materials Crack Repair.	Son HM, Kim HY, Park SM, Lee HK.	Materials (Basel)	10.3390/ma11050782	2018
Enzymology	Engineered Ureolytic Microorganisms Can Tailor the Morphology and Nanomechanical Properties of Microbial-Precipitated Calcium Carbonate.	Heveran CM, Liang L, Nagarajan A, Hubler MH, Gill R, Cameron JC, Cook SM, Srubar WV.	Sci Rep	10.1038/s41598-019-51133-9	2019
	Quantifying spatiotemporal variability and noise in absolute microbiota abundances using replicate sampling.	Ji BW, Sheth RU, Dixit PD, Huang Y, Kaufman A, Wang HH, Vitkup D.	Nat Methods	10.1038/s41592-019-0467-y	2019
	Biomineralization of Plastic Waste to Improve the Strength of Plastic-Reinforced Cement Mortar.	Kane S, Thane A, Espinal M, Lunday K, Armagan H, Phillips A, Heveran C, Ryan C.	Materials (Basel)	10.3390/ma14081949	2021
Enzymology	Application of stereolithographic custom models for studying the impact of biofilms and mineral precipitation on fluid flow.	Stoner DL, Watson SM, Stedtfeld RD, Meakin P, Griffel LK, Tyler TL, Pegram LM, Barnes JM, Deason VA.	Appl Environ Microbiol	10.1128/aem.71.12.8721-8728.2005	2005
	Soil engineering in vivo: harnessing natural biogeochemical systems for sustainable, multi-functional engineering solutions.	DeJong JT, Soga K, Banwart SA, Whalley WR, Ginn TR, Nelson DC, Mortensen BM, Martinez BC, Barkouki T.	J R Soc Interface	10.1098/rsif.2010.0270	2011
Phylogeny	Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks.	Lasch P, Beyer W, Nattermann H, Stammler M, Siegbrecht E, Grunow R, Naumann D.	Appl Environ Microbiol	10.1128/aem.00857-09	2009
	Halogenated N-Benzylbenzisoselenazolones Efficiently Inhibit Helicobacter pylori Ureolysis In Vitro.	Grabarek M, Tabor W, Krzyzek P, Grabowiecka A, Berlicki L, Mucha A.	ACS Med Chem Lett	10.1021/acsmedchemlett.5c00057	2025
	Exploration of Thiourea-Based Scaffolds for the Construction of Bacterial Ureases Inhibitors.	Tabor W, Katsogiannou A, Karta D, Andrianopoulou E, Berlicki L, Vassiliou S, Grabowiecka A.	ACS Omega	10.1021/acsomega.3c03702	2023
	High level of calcium carbonate precipitation achieved by mixed culture containing ureolytic and non-ureolytic bacterial strains.	Harnpicharnchai P, Mayteeworakoon S, Kitikhun S, Chunhametha S, Likhitrattanapisal S, Eurwilaichitr L, Ingsriswang S	Lett Appl Microbiol	10.1111/lam.13748	2022
	Best-performing Bacillus strains for microbiologically induced CaCO3 precipitation: Screening of relative influence of operational and environmental factors.	Sovljanski O, Pezo L, Grahovac J, Tomic A, Ranitovic A, Cvetkovic D, Markov S	J Biotechnol	10.1016/j.jbiotec.2022.04.002	2022
	Comprehensive Profiling of Microbiologically Induced CaCO3 Precipitation by Ureolytic Bacillus Isolates from Alkaline Soils.	Sovljanski O, Pezo L, Stanojev J, Bajac B, Kovac S, Toth E, Ristic I, Tomic A, Ranitovic A, Cvetkovic D, Markov S	Microorganisms	10.3390/microorganisms9081691	2021
	Biocementation mediated by native microbes from Brahmaputra riverbank for mitigation of soil erodibility.	Dubey AA, Ravi K, Mukherjee A, Sahoo L, Abiala MA, Dhami NK	Sci Rep	10.1038/s41598-021-94614-6	2021
Metabolism	Towards a low CO2 emission building material employing bacterial metabolism (1/2): The bacterial system and prototype production.	Royne A, Phua YJ, Balzer Le S, Eikjeland IG, Josefsen KD, Markussen S, Myhr A, Throne-Holst H, Sikorski P, Wentzel A	PLoS One	10.1371/journal.pone.0212990	2019
Metabolism	Improvement of Biomineralization of Sporosarcina pasteurii as Biocementing Material for Concrete Repair by Atmospheric and Room Temperature Plasma Mutagenesis and Response Surface Methodology.	Han PP, Geng WJ, Li MN, Jia SR, Yin JL, Xue RZ	J Microbiol Biotechnol	10.4014/jmb.2104.04019	2021
Metabolism	Contribution of bacterial cells as nucleation centers in microbiologically induced CaCO(3) precipitation-A mathematical modeling approach.	Sovljanski O, Pezo L, Tomic A, Ranitovic A, Cvetkovic D, Markov S	J Basic Microbiol	10.1002/jobm.202100275	2021
Metabolism	Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media.	Lapierre FM, Schmid J, Ederer B, Ihling N, Buchs J, Huber R	Sci Rep	10.1038/s41598-020-79904-9	2020
Metabolism	Facultative and anaerobic consortia of haloalkaliphilic ureolytic micro-organisms capable of precipitating calcium carbonate.	Skorupa DJ, Akyel A, Fields MW, Gerlach R	J Appl Microbiol	10.1111/jam.14384	2019
	Diversity of Sporosarcina-like Bacterial Strains Obtained from Meter-Scale Augmented and Stimulated Biocementation Experiments.	Graddy CMR, Gomez MG, Kline LM, Morrill SR, DeJong JT, Nelson DC	Environ Sci Technol	10.1021/acs.est.7b04271	2018
	Microbially Induced Calcite Precipitation Employing Environmental Isolates.	Kim G, Youn H	Materials (Basel)	10.3390/ma9060468	2016
Metabolism	Effects of bentonite and yeast extract as nutrient on decrease in hydraulic conductivity of porous media due to CaCO3 precipitation induced by Sporosarcina pasteurii.	Eryuruk K, Yang S, Suzuki D, Sakaguchi I, Katayama A	J Biosci Bioeng	10.1016/j.jbiosc.2015.01.020	2015
Metabolism	Reducing hydraulic conductivity of porous media using CaCO(3) precipitation induced by Sporosarcina pasteurii.	Eryuruk K, Yang S, Suzuki D, Sakaguchi I, Akatsuka T, Tsuchiya T, Katayama A	J Biosci Bioeng	10.1016/j.jbiosc.2014.08.009	2014
Metabolism	Biocontainment of polychlorinated biphenyls (PCBs) on flat concrete surfaces by microbial carbonate precipitation.	Okwadha GD, Li J	J Environ Manage	10.1016/j.jenvman.2011.05.029	2011
Metabolism	Optimum conditions for microbial carbonate precipitation.	Okwadha GD, Li J	Chemosphere	10.1016/j.chemosphere.2010.09.066	2010
	Regulation of leucine transport by intracellular pH in Bacillus pasteurii.	Beck L, Jahns T	Arch Microbiol	10.1007/s002030050325	1996
Metabolism	Ammonium/urea-dependent generation of a proton electrochemical potential and synthesis of ATP in Bacillus pasteurii.	Jahns T	J Bacteriol	10.1128/jb.178.2.403-409.1996	1996
Enzymology	Nickel-content of urease from Bacillus pasteurii.	Christians S, Kaltwasser H	Arch Microbiol	10.1007/BF00413026	1986
Phylogeny	Sporosarcina jiandibaonis sp. nov., isolated from saline soil.	Kong D, Xia Z, Gao Y, Zhou Y, Jiang X, Xu P, Guo W, Ruan Z.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005244	2022

References

#2034	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 33
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#41674	; Curators of the CIP;
#44994	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 7425
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121627	Collection of Institut Pasteur ; Curators of the CIP; CIP 66.21
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Sporosarcina pasteurii (DSM 33, ATCC 11859, CCM 2056)

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