Name: Thiomicrospira microaerophila ASL8-2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 11913

Type strain:

Species: Thiomicrospira microaerophila

Strain Designation: ASL8-2

Culture col. no.: DSM 17327, UNIQEM U242

Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ASL8-2

NCBI tax ID(s): 406020 (species)

SI-ID 408020

DSM 17327

Special Collections

Section

Thiomicrospira microaerophila ASL8-2 is a microaerophile, chemolithoautotroph, motile bacterium that was isolated from oxygen-sulfide interface water.

motile
pleomorphic-shaped
chemolithoautotroph
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Beggiatoales*
	Family	*Piscirickettsiaceae*
	Genus	*Thiomicrospira*
	Species	**Thiomicrospira microaerophila**
	Full Scientific Name (LPSN)	Thiomicrospira microaerophila (Sorokin et al. 2007) Boden et al. 2017

Synonym

Thioalkalimicrobium microaerophilum

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.333

[Ref.: #43182]	Cell length	2.0-5.0 µm

[Ref.: #43182]	Cell width	1.0-2.0 µm

[Ref.: #43182]	Cell shape	pleomorphic-shaped

[Ref.: #43182]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6895]

Culture medium

THIOALKALIMICROBIUM MICROAEOPHILUM MEDIUM (DSMZ Medium 1230)

[Ref.: #6895]

Culture medium growth

[Ref.: #6895]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6895]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #6895]			growth	28 °C
[Ref.: #43182]			optimum	25-28 °C

		pH
[Ref.: #43182]	maximum	10
[Ref.: #43182]	minimum	8
[Ref.: #43182]	optimum	9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6895]	Oxygen tolerance	microaerophile
[Ref.: #43182]	Oxygen tolerance	obligate aerobe

[Ref.: #43182]

Nutrition type

chemolithoautotroph

[Ref.: #69480]	Ability of spore formation	no Confidence in %92.691
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Salt		Salt conc.
[Ref.: #43182]	NaCl	optimum	0.6	M
[Ref.: #43182]	NaCl	minimum	0.2	M
[Ref.: #43182]	NaCl	maximum	1.2	M

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43182]	dihydrogen ChEBI	-	electron donor
[Ref.: #43182]	elemental sulfur ChEBI	+	electron donor
[Ref.: #43182]	iron ChEBI	-	electron donor
[Ref.: #43182]	manganese ChEBI	-	electron donor
[Ref.: #43182]	sulfide ChEBI	+	electron donor
[Ref.: #43182]	sulfite ChEBI	-	electron donor
[Ref.: #43182]	tetrathionate ChEBI	-	energy source
[Ref.: #43182]	tetrathionate ChEBI	+	electron donor
[Ref.: #43182]	thiocyanate ChEBI	-	electron donor
[Ref.: #43182]	thiosulfate ChEBI	+	electron donor
[Ref.: #43182]	trithionate ChEBI	+	electron donor
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43182]		cobalamin ChEBI	yes
[Ref.: #43182]		elemental sulfur ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43182]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6895]	Sample type/isolated from	oxygen-sulfide interface water
[Ref.: #6895]	Geographic location (country and/or sea, region)	Washington State, Grant County, Soap Lake
[Ref.: #6895]	Country	USA
[Ref.: #6895]	Country ISO 3 Code	USA
[Ref.: #6895]	Continent	North America

[Ref.: #43182]	Sample type/isolated from	Soap Lake
[Ref.: #43182]	Geographic location (country and/or sea, region)	Washington, USA
[Ref.: #43182]	Country	USA
[Ref.: #43182]	Country ISO 3 Code	USA
[Ref.: #43182]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Condition	#Sulfuric	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ900623 (>99% sequence identity) for Thiomicrospira microaerophila subclade from Microbeatlas

Aquatic Samples	66
Soil Samples	4
Animal Samples
Plant Samples
Total Samples	70

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6895]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6895]	Thioalkalimicrobium microaerophilum strain ASL8-2 16S ribosomal RNA gene, partial sequence	DQ900623	1366	GenBank ENA	406020 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thiomicrospira microaerophila ASL8-2	GCA_000934765	scaffold	GenBank ENA	406020 tax ID	*
[Ref.: #66792]	Thiomicrospira microaerophila ASL8-2	2593339162	draft	JGI IMG/M	406020 tax ID	*

[Ref.: #43182]

GC-content

45.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thiomicrospira microaerophila ASL8-2 NCBI: GCA_000934765.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thiomicrospira microaerophila ASL8-2 NCBI: GCA_000934765.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99.333	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	84.498	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	50.858	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.691	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.339	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	77.698	no

Availability in culture collections External links

[Ref.: #6895]

Culture collection no.

DSM 17327, UNIQEM U242

[Ref.: #81127]

SI-ID 408020

References

#6895

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17327

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43182

Rich Boden, Kathleen M. Scott, John Williams, Sydney Russel, Kirsten Antonen, Alex W. Rae and Lee P. Hutt: An evaluation of Thiomicrospira, Hydrogenovibrio and Thioalkalimicrobium: reclassification of four species of Thiomicrospira to each Thiomicrorhabdus gen. nov. and Hydrogenovibrio, and reclassification of all four species of Thioalkalimicrobium to Thiomicrospira. IJSEM 67: 1140 - 1151 2017 ( DOI 10.1099/ijsem.0.001855 , PubMed 28581925 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81127

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408020.1 )

* These data were automatically processed and therefore are not curated

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