Name: Actinobacillus pleuropneumoniae 4074
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 11672

Type strain:

Species: Actinobacillus pleuropneumoniae

Strain Designation: 4074

Culture col. no.: DSM 13472, ATCC 27088, CCM 5869, Shope 4074, CIP 100916, CCUG 41656

Strain history: CIP <- 1984, ATCC <- J. Nicolet, Haemophilus parahaemolyticus: strain 4074 <- J. Callis <- R. Shope: strain 4074

NCBI tax ID(s): 715 (species)

SI-ID 38804

ATCC 27088, CCM 5869, CCUG 12837, CIP 100916, CCUG 41656, DSM 13472, CCUG 43096, NCTC 12370

Special Collections

Other strains from Actinobacillus pleuropneumoniae

Section

Actinobacillus pleuropneumoniae 4074 is a microaerophile animal pathogen that was isolated from lung of swine.

animal pathogen
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Actinobacillus*
	Species	**Actinobacillus pleuropneumoniae**
	Full Scientific Name (LPSN)	Actinobacillus pleuropneumoniae (Shope 1964) Pohl et al. 1983

	Synonym	Haemophilus pleuropneumoniae
	Synonym	Hemophilus pleuropneumoniae

Information on morphological and physiological properties Morphology

[Ref.: #121870]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98

[Ref.: #121870]	Cell shape	oval-shaped

[Ref.: #69480]	Motility	no Confidence in %92.821

[Ref.: #5054]	Type of hemolysis	gamma
[Ref.: #5054]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5054]

Culture medium

HAEMOPHILUS MEDIUM (DSMZ Medium 804)

[Ref.: #5054]

Culture medium growth

[Ref.: #5054]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5054]

Culture medium composition

expand / minimize

[Ref.: #39724]

Culture medium

MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria

[Ref.: #39724]

Culture medium growth

[Ref.: #39724]

Culture medium composition

expand / minimize

[Ref.: #121870]

Culture medium

CIP Medium 10

[Ref.: #121870]

Culture medium growth

[Ref.: #121870]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #5054]	growth	37 °C
[Ref.: #39724]	growth	37 °C
[Ref.: #121870]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5054]

Oxygen tolerance

microaerophile

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	D-fructose ChEBI	+	builds acid from	* from API NH
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	maltose ChEBI	+	builds acid from	* from API NH
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #121870]	nitrate ChEBI	+	reduction
[Ref.: #121870]	nitrite ChEBI	-	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121870]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	sucrose ChEBI	+	builds acid from	* from API NH
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	urea ChEBI	+	hydrolysis	* from API NH
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121870]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #121870]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #121870]	acetoin ChEBI	-
[Ref.: #121870]	glucose ChEBI		-
[Ref.: #68377]	indole ChEBI			-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #121870]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68377]	alkaline phosphatase	+	3.1.3.1	* from API NH
[Ref.: #121870]	beta-galactosidase	+	3.2.1.23
[Ref.: #68377]	beta-galactosidase	+	3.2.1.23	* from API NH
[Ref.: #68377]	beta-lactamase	-	3.5.2.6	* from API NH
[Ref.: #5054]	catalase	-	1.11.1.6
[Ref.: #121870]	catalase	+	1.11.1.6
[Ref.: #5054]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #121870]	gelatinase	-
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #121870]	lysine decarboxylase	-	4.1.1.18
[Ref.: #121870]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #121870]	oxidase	+
[Ref.: #68377]	proline-arylamidase	-	3.4.11.5	* from API NH
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #121870]	urease	+	3.5.1.5
[Ref.: #68377]	urease	+	3.5.1.5	* from API NH
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121870]	8695	not determinedn.d.	-	-	-	+	+/-	+/-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+/-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #5054]	6258	-	+	+	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5054]	Sample type/isolated from	lung of swine
[Ref.: #5054]	Country	Argentina
[Ref.: #5054]	Country ISO 3 Code	ARG
[Ref.: #5054]	Continent	Middle and South America

[Ref.: #121870]	Sample type/isolated from	Animal, Pig, lung
[Ref.: #121870]	Country	Argentina
[Ref.: #121870]	Country ISO 3 Code	ARG
[Ref.: #121870]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body-Site	#Oral cavity and airways	#Lung

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence D30030 (>99% sequence identity) for Actinobacillus from Microbeatlas

Aquatic Samples	203
Soil Samples	221
Animal Samples	5580
Plant Samples	19
Total Samples	6023

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5054]	Pathogenicity (animal)	yes
[Ref.: #5054]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121870]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5054]	Actinobacillus pleuropneumoniae strain ATCC 27088 16S ribosomal RNA gene, partial sequence	M75074	1478	GenBank ENA	715 tax ID
[Ref.: #20218]	Actinobacillus pleuropneumoniae gene for 16S rRNA	D30030	1743	GenBank ENA	715 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinobacillus pleuropneumoniae strain S4074	715.77	complete	PATRIC	715 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinobacillus pleuropneumoniae strain S4074 PATRIC: 715.77
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.812	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.522	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	80.144	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	92.821	no

Availability in culture collections External links

[Ref.: #5054]

Culture collection no.

DSM 13472, ATCC 27088, CCM 5869, Shope 4074, CIP 100916, CCUG 41656

[Ref.: #80893]

SI-ID 38804

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice.	Shao M, Wang Y, Wang C, Guo Y, Peng Y, Liu J, Li G, Liu H, Liu S	Acta Vet Scand	10.1186/1751-0147-52-52	2010	*
Metabolism	Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae.	Schaller A, Kuhn R, Kuhnert P, Nicolet J, Anderson TJ, Maclnnes JI, Segers RPAM, Frey J	Microbiology (Reading)	10.1099/13500872-145-8-2105	1999	*
Metabolism	First chromosomal restriction map of Actinobacillus pleuropneumoniae and localization of putative virulence-associated genes.	Oswald W, Konine DV, Rohde J, Gerlach GF	J Bacteriol	10.1128/JB.181.14.4161-4169.1999	1999	*
Phylogeny	Characterization of monoclonal antibodies that recognize common epitopes located on O antigen of lipopolysaccharide of serotypes 1, 9 and 11 of Actinobacillus pleuropneumoniae.	Rodriguez Barbosa JI, Gutierrez Martin CB, Tascon RI, Gonzalez OR, Mittal KR, Rodriguez Ferri EF	FEMS Immunol Med Microbiol	10.1111/j.1574-695X.1996.tb00134.x	1996	*
Phylogeny	Comparative study of iron acquisition by biotype 1 and biotype 2 strains of Actinobacillus pleuropneumoniae.	D'Silva CG, Archibald FS, Niven DF	Vet Microbiol	10.1016/0378-1135(94)00109-a	1995	*
Phylogeny	Efficacy of florfenicol on experimental Actinobacillus pleuropneumonia in pigs.	Ueda Y, Ohtsuki S, Narukawa N	J Vet Med Sci	10.1292/jvms.57.261	1995	*
Enzymology	Lipopolysaccharides of Actinobacillus pleuropneumoniae (serotype 1): a readily obtainable antigen for ELISA serodiagnosis of pig pleuropneumonia.	Radacovici S, Gottschalk M, Dubreuil JD	Vet Microbiol	10.1016/0378-1135(94)90159-7	1994	*
Enzymology	Antigenic differences within Actinobacillus pleuropneumoniae serotype 1.	Jolie RA, Mulks MH, Thacker BJ	Vet Microbiol	10.1016/0378-1135(94)90151-1	1994	*
Enzymology	Clonal analysis of the Actinobacillus pleuropneumoniae population in a geographically restricted area by multilocus enzyme electrophoresis.	Moller K, Nielsen R, Andersen LV, Kilian M	J Clin Microbiol	10.1128/jcm.30.3.623-627.1992	1992	*
Metabolism	Calcium binds to and is required for biological activity of the 104-kilodalton hemolysin produced by Actinobacillus pleuropneumoniae serotype 1.	Devenish J, Rosendal S	Can J Microbiol	10.1139/m91-050	1991	*
Phylogeny	Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype 1.	Altman E, Brisson JR, Perry MB	Biochem Cell Biol	10.1139/o86-097	1986	*
Phylogeny	Comparative virulence of porcine Haemophilus bacteria.	Rosendal S, Boyd DA, Gilbride KA	Can J Comp Med		1985	*
Phylogeny	Haemophilus pleuropneumoniae serotyping.	Rosendal S, Boyd DA	J Clin Microbiol	10.1128/jcm.16.5.840-843.1982	1982	*
	Passive protection of piglets by monoclonal antibodies against experimental infection with Actinobacillus pleuropneumoniae.	Smith IK, Lida J	Res Vet Sci		1990	*

References

#5054

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13472

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39724 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68377

Automatically annotated from API NH .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80893

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38804.1 )

#121870 Collection of Institut Pasteur ; Curators of the CIP; CIP 100916
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Actinobacillus pleuropneumoniae in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Actinobacillus pleuropneumoniae strain S4074

Change proposal

Change proposal