Strain identifier

BacDive ID: 11672

Type strain: Yes

Species: Actinobacillus pleuropneumoniae

Strain Designation: 4074

Strain history: CIP <- 1984, ATCC <- J. Nicolet, Haemophilus parahaemolyticus: strain 4074 <- J. Callis <- R. Shope: strain 4074

NCBI tax ID(s): 715 (species)

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General

@ref: 5054

BacDive-ID: 11672

DSM-Number: 13472

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, oval-shaped, animal pathogen

description: Actinobacillus pleuropneumoniae 4074 is a microaerophile, mesophilic, Gram-negative animal pathogen that was isolated from lung of swine.

NCBI tax id

  • NCBI tax id: 715
  • Matching level: species

strain history

@refhistory
5054<- ATCC; <- J. Nicolet; Shope 4074 (Haemophilus parahaemolyticus) <- J. Callis; <- R. E. Shope;
121870CIP <- 1984, ATCC <- J. Nicolet, Haemophilus parahaemolyticus: strain 4074 <- J. Callis <- R. Shope: strain 4074

doi: 10.13145/bacdive11672.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus pleuropneumoniae
  • full scientific name: Actinobacillus pleuropneumoniae (Shope 1964) Pohl et al. 1983

  • synonyms

    @refsynonym
    20215Haemophilus pleuropneumoniae
    20215Hemophilus pleuropneumoniae

@ref: 5054

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus pleuropneumoniae

full scientific name: Actinobacillus pleuropneumoniae (Shope 1964) Pohl et al. 1983

strain designation: 4074

type strain: yes

Morphology

cell morphology

  • @ref: 121870
  • gram stain: negative
  • cell shape: oval-shaped

colony morphology

@reftype of hemolysisincubation period
5054gamma1-2 days
121870

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5054HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
39724MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
121870CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
5054positivegrowth37mesophilic
39724positivegrowth37mesophilic
121870positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5054
  • oxygen tolerance: microaerophile

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121870nitrate+reduction17632
121870nitrite-reduction16301
121870sodium thiosulfate-builds gas from132112
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377sucrose+builds acid from17992
68377ornithine-degradation18257
68377urea+hydrolysis16199
68377tryptophan-energy source27897

antibiotic resistance

  • @ref: 121870
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12187035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6837735581indole-
12187015688acetoin-
12187017234glucose-

enzymes

@refvalueactivityec
5054catalase-1.11.1.6
5054cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase+3.2.1.23
68377alkaline phosphatase+3.1.3.1
68377lipase-
68377urease+3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
121870oxidase+
121870beta-galactosidase+3.2.1.23
121870alcohol dehydrogenase-1.1.1.1
121870gelatinase-
121870catalase+1.11.1.6
121870lysine decarboxylase-4.1.1.18
121870ornithine decarboxylase-4.1.1.17
121870urease+3.5.1.5

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121870---++/-+/----+-----------+-++/--+--------------------++

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
5054-++++-+-++---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5054lung of swineArgentinaARGMiddle and South America
121870Animal, Pig, lungArgentinaARGMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Oral cavity and airways#Lung

taxonmaps

  • @ref: 69479
  • File name: preview.99_217.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_155;97_166;98_185;99_217&stattab=map
  • Last taxonomy: Actinobacillus
  • 16S sequence: D30030
  • Sequence Identity:
  • Total samples: 6023
  • soil counts: 221
  • aquatic counts: 203
  • animal counts: 5580
  • plant counts: 19

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
5054yes2Risk group (German classification)
1218701Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus pleuropneumoniae gene for 16S rRNAD300301743ena715
5054Actinobacillus pleuropneumoniae strain ATCC 27088 16S ribosomal RNA gene, partial sequenceM750741478ena715

Genome sequences

  • @ref: 66792
  • description: Actinobacillus pleuropneumoniae strain S4074
  • accession: 715.77
  • assembly level: complete
  • database: patric
  • NCBI tax ID: 715

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.276no
anaerobicno97.684yes
halophileno62.877no
spore-formingno98.837no
glucose-utilno50no
thermophileno98.311yes
flagellatedno94.399no
aerobicno89.721no
glucose-fermentno51.767no
motileno91.003no

External links

@ref: 5054

culture collection no.: DSM 13472, ATCC 27088, CCM 5869, Shope 4074, CIP 100916, CCUG 41656

straininfo link

  • @ref: 80893
  • straininfo: 38804

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1551979Clonal analysis of the Actinobacillus pleuropneumoniae population in a geographically restricted area by multilocus enzyme electrophoresis.Moller K, Nielsen R, Andersen LV, Kilian MJ Clin Microbiol10.1128/jcm.30.3.623-627.19921992Actinobacillus pleuropneumoniae/classification/enzymology/*genetics, Animals, Denmark, Electrophoresis, Starch Gel, Enzymes/genetics/isolation & purification, Genetic Variation, Serotyping, Swine/*microbiologyPhylogeny
Metabolism1913343Calcium binds to and is required for biological activity of the 104-kilodalton hemolysin produced by Actinobacillus pleuropneumoniae serotype 1.Devenish J, Rosendal SCan J Microbiol10.1139/m91-0501991Actinobacillus/*metabolism, Animals, Autoradiography, Calcium/*metabolism/pharmacology, Culture Media, Electrophoresis, Polyacrylamide Gel, Hemolysin Proteins/*metabolism, Hemolysis/*drug effects, ImmunoblottingCultivation
2236908Passive protection of piglets by monoclonal antibodies against experimental infection with Actinobacillus pleuropneumoniae.Smith IK, Lida JRes Vet Sci1990Actinobacillus Infections/prevention & control/*veterinary, Acute Disease, Administration, Intranasal, Animals, Antibodies, Monoclonal/administration & dosage/*therapeutic use, Immunization, Passive/*veterinary, Injections, Intraperitoneal/veterinary, Pleuropneumonia/prevention & control/*veterinary, Swine, Swine Diseases/*prevention & control
Phylogeny3768164Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype 1.Altman E, Brisson JR, Perry MBBiochem Cell Biol10.1139/o86-0971986Chemical Phenomena, Chemistry, Galactose/analysis, Glucosamine/analysis, Haemophilus/*analysis/classification, Hydrolysis, Magnetic Resonance Spectroscopy, Methylation, Phosphates/analysis, Phosphorylation, *Polysaccharides, Bacterial/analysis, Serotyping
Phylogeny3986682Comparative virulence of porcine Haemophilus bacteria.Rosendal S, Boyd DA, Gilbride KACan J Comp Med1985Animals, Arthritis/microbiology/pathology/veterinary, Haemophilus/classification/isolation & purification/*pathogenicity, Haemophilus Infections/pathology/*veterinary, Lung/pathology, Pleuropneumonia/microbiology/pathology/*veterinary, Swine/microbiology, Swine Diseases/*microbiology/pathology, VirulenceEnzymology
Phylogeny7153336Haemophilus pleuropneumoniae serotyping.Rosendal S, Boyd DAJ Clin Microbiol10.1128/jcm.16.5.840-843.19821982Agglutination Tests, Animals, Antigens, Viral/analysis, Cross Reactions, Haemophilus/*classification/immunology, Rabbits, Serotyping, Swine
Phylogeny7492643Efficacy of florfenicol on experimental Actinobacillus pleuropneumonia in pigs.Ueda Y, Ohtsuki S, Narukawa NJ Vet Med Sci10.1292/jvms.57.2611995Actinobacillus Infections/*drug therapy/pathology/physiopathology, *Actinobacillus pleuropneumoniae/classification/drug effects, Animals, Anti-Bacterial Agents/*therapeutic use, Drug Resistance, Microbial, Lung/pathology, Serotyping, Swine, *Swine Diseases, Thiamphenicol/*analogs & derivatives/pharmacology/therapeutic usePathogenicity
Phylogeny7667902Comparative study of iron acquisition by biotype 1 and biotype 2 strains of Actinobacillus pleuropneumoniae.D'Silva CG, Archibald FS, Niven DFVet Microbiol10.1016/0378-1135(94)00109-a1995Actinobacillus pleuropneumoniae/classification/growth & development/*metabolism, Animals, Bacterial Proteins/isolation & purification/*metabolism, Carrier Proteins/isolation & purification/*metabolism, Cattle, Hemoglobins/isolation & purification/metabolism, Humans, Iron/*metabolism, Lactoferrin/isolation & purification/metabolism, Membrane Proteins/isolation & purification/metabolism, Species Specificity, Swine, Transferrin/isolation & purification/metabolismMetabolism
Enzymology8042270Lipopolysaccharides of Actinobacillus pleuropneumoniae (serotype 1): a readily obtainable antigen for ELISA serodiagnosis of pig pleuropneumonia.Radacovici S, Gottschalk M, Dubreuil JDVet Microbiol10.1016/0378-1135(94)90159-71994Actinobacillus Infections/diagnosis/*veterinary, Actinobacillus pleuropneumoniae/*immunology, Animals, Antigens, Bacterial/immunology/isolation & purification, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay/veterinary, Immune Sera/immunology, Immunoblotting, Lipopolysaccharides/*immunology/isolation & purification, Pleuropneumonia/diagnosis/*veterinary, Sensitivity and Specificity, Swine, Swine Diseases/*diagnosisPhylogeny
Enzymology8160348Antigenic differences within Actinobacillus pleuropneumoniae serotype 1.Jolie RA, Mulks MH, Thacker BJVet Microbiol10.1016/0378-1135(94)90151-11994Actinobacillus Infections/diagnosis/immunology/*veterinary, Actinobacillus pleuropneumoniae/*classification/immunology, Agglutination Tests, Animals, Antibodies, Bacterial/blood, Antigens, Bacterial/*analysis/immunology, Bacterial Outer Membrane Proteins/*analysis/immunology, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Immunization, Immunoblotting, Lipopolysaccharides/*analysis/immunology/isolation & purification, Male, Molecular Weight, Pleuropneumonia/diagnosis/immunology/*veterinary, Rabbits/immunology, Serotyping, Swine, *Swine DiseasesPhylogeny
Phylogeny9116634Characterization of monoclonal antibodies that recognize common epitopes located on O antigen of lipopolysaccharide of serotypes 1, 9 and 11 of Actinobacillus pleuropneumoniae.Rodriguez Barbosa JI, Gutierrez Martin CB, Tascon RI, Gonzalez OR, Mittal KR, Rodriguez Ferri EFFEMS Immunol Med Microbiol10.1111/j.1574-695X.1996.tb00134.x1996Actinobacillus pleuropneumoniae/*classification/immunology, Agglutination Tests, Animals, Antibodies, Bacterial/*immunology, Antibodies, Monoclonal, *Antibody Specificity, Cross Reactions, Enzyme-Linked Immunosorbent Assay, *Epitopes, Immunoglobulin Isotypes, Mice, Mice, Inbred BALB C, O Antigens/*immunology, Serotyping/standards, Species Specificity
Metabolism10400571First chromosomal restriction map of Actinobacillus pleuropneumoniae and localization of putative virulence-associated genes.Oswald W, Konine DV, Rohde J, Gerlach GFJ Bacteriol10.1128/JB.181.14.4161-4169.19991999Actinobacillus pleuropneumoniae/classification/*genetics/pathogenicity, Blotting, Southern, Chromosome Mapping, DNA, Bacterial/metabolism, Deoxyribonucleases, Type II Site-Specific/metabolism, Electrophoresis, Gel, Pulsed-Field, *Genome, Bacterial, *Restriction Mapping, Virulence/*geneticsEnzymology
Metabolism10463177Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae.Schaller A, Kuhn R, Kuhnert P, Nicolet J, Anderson TJ, Maclnnes JI, Segers RPAM, Frey JMicrobiology (Reading)10.1099/13500872-145-8-21051999Actinobacillus Infections/immunology/microbiology/veterinary, Actinobacillus pleuropneumoniae/chemistry/*genetics/metabolism, Animals, Antibodies, Bacterial/blood, Bacterial Proteins/chemistry/*genetics/immunology/metabolism, Blotting, Southern, Calcium/metabolism, Cloning, Molecular, Epitopes, Gene Expression, Genes, Bacterial, Molecular Sequence Data, Recombinant Fusion Proteins/genetics/immunology/metabolism, Sequence Analysis, DNA, Swine, Swine Diseases/immunology/microbiologyEnzymology
Pathogenicity20831818Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice.Shao M, Wang Y, Wang C, Guo Y, Peng Y, Liu J, Li G, Liu H, Liu SActa Vet Scand10.1186/1751-0147-52-522010Actinobacillus Infections/microbiology/mortality/prevention & control/*veterinary, Actinobacillus pleuropneumoniae/*immunology, Animals, Antibodies, Bacterial/blood, Bacterial Toxins/genetics/*immunology, Bacterial Vaccines/genetics/*immunology, Fluorescent Antibody Technique, Indirect/veterinary, Immunization/methods/standards/*veterinary, Male, Mice, Mice, Inbred BALB C, Random Allocation, Recombinant Proteins/genetics/immunology, Swine, Swine Diseases/immunology/*microbiology/prevention & control, Vaccines, Subunit/genetics/immunology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5054Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13472)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13472
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39724Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12433
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80893Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38804.1StrainInfo: A central database for resolving microbial strain identifiers
121870Curators of the CIPCollection of Institut Pasteur (CIP 100916)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20100916