Paenibacillus nematophilus (DSM 13559, CIP 108049, NEM1a)

Name: Paenibacillus nematophilus (DSM 13559, CIP 108049, NEM1a)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11562

Type strain

Strain Designation NEM1a

Culture col. no. DSM 13559 CIP 108049 NEM1a NCIMB 13845

NCBI tax ID(s) 200132

Special Collections DSMZ CIP

History <- M. R. Enright; NEM1a CIP <- 2013, DSMZ <- M.R. Enright: strain NEM1a

Links

Paenibacillus nematophilus NEM1a is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from entomopathogenic nematode, Heterorhabditis megidis EU17.

Gram-negative rod-shaped facultative anaerobe mesophilic animal pathogen 16S sequence

Name and taxonomic classification

SI-ID 100840

DSM 13559,NCIMB 13845,CCM 7119,KCTC 3830,BCRC 17353,CCRC 17353,LMG24734,CIP 108049

@ref 20215

Last LPSN update 2026-02-09 11:20:35

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Paenibacillus

Species Paenibacillus nematophilus

Full scientific name Paenibacillus nematophilus Enright et al. 2003

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121263	negative	rod-shaped

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5087	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535; with strain-specific modifications) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l MnSO4 0.01 g/l Distilled water
36539	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121263	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5087	positive	growth	30	mesophilic
36539	positive	growth	30	mesophilic
121263	positive	growth	22-37
121263	negative	growth	10
121263	negative	growth	45
121263	negative	growth	55

Culture pH

@ref	Ability	Type	PH
121263	positive	growth	6

Physiology and metabolism

Oxygen tolerance

121263

Oxygen tolerancefacultative anaerobe

Spore formation

121263

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
121263	NaCl	positive	growth	0 %
121263	NaCl		growth	2 %
121263	NaCl		growth	4 %
121263	NaCl		growth	6 %
121263	NaCl		growth	8 %
121263	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
121263	16947 ChEBI	citrate	-	carbon source
121263	4853 ChEBI	esculin	-	hydrolysis
121263	606565 ChEBI	hippurate	+	hydrolysis
121263	17632 ChEBI	nitrate	-	reduction
121263	17632 ChEBI	nitrate	-	respiration
121263	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121263	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
121263	15688 ChEBI	acetoin	+
121263	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
121263	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121263	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
121263	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121263	caseinase	-	3.4.21.50
121263	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
121263	DNase	-
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
121263	gamma-glutamyltransferase	-	2.3.2.2
121263	gelatinase	-
121263	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121263	lipase	-
68382	lipase (C 14)	-		from API zym
121263	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121263	ornithine decarboxylase	-	4.1.1.17
121263	oxidase	-
121263	protease	-
68382	trypsin	-	3.4.21.4	from API zym
121263	tween esterase	-
121263	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121263	-	-	-	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Invertebrates (Other)	#Nematoda

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent
5087	entomopathogenic nematode, Heterorhabditis megidis EU17	Heterorhabditis megidis	Estonia	EST	Europe
121263	Animal, Insect pathogenic nematode, Heterorhabditis megidis		Estonia	EST	Europe

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
5087		1	Risk group (German classification) According to TRBA 466
121263		1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Paenibacillus nematophilus strain NEM1a 16S ribosomal RNA gene, partial sequence	AF480935	1532		200132

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.	Enright MR, McInerney JO, Griffin CT	Int J Syst Evol Microbiol	10.1099/ijs.0.02344-0	2003

References

#5087	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13559
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#36539	; Curators of the CIP;
#68382	Automatically annotated from API zym .
#121263	Collection of Institut Pasteur ; Curators of the CIP; CIP 108049
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Paenibacillus nematophilus (DSM 13559, CIP 108049, NEM1a)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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