Name: Paenibacillus lactis MB 1871
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11563

Type strain:

Species: Paenibacillus lactis

Strain Designation: MB 1871, R-7191

Culture col. no.: DSM 15596, CIP 108827, LMG 21940

Strain history: CIP <- 2005, DSMZ <- P. Scheldeman, CLO, Belgium: strain MB 1871

NCBI tax ID(s): 1255631 (strain), 228574 (species)

SI-ID 97185

LMG 21910, LMG 21940, DSM 15596, CIP 108827

Other strains from Paenibacillus lactis

P. lactis ptZ-260P, DSM 28626
P. lactis CCUG 31365
P. lactis CCUG 41748
P. lactis CCUG 50157
P. lactis CIMB 04-2903, CRBIP17.124

Section

Paenibacillus lactis MB 1871 is an aerobe, spore-forming, mesophilic bacterium that was isolated from raw milk.

Gram-variable
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus lactis**
	Full Scientific Name (LPSN)	Paenibacillus lactis Scheldeman et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #30009]	Gram stain	variable
[Ref.: #120533]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #30009]	Cell length	4.5 µm

[Ref.: #30009]	Cell width	0.75 µm

[Ref.: #30009]	Cell shape	rod-shaped
[Ref.: #120533]	Cell shape	rod-shaped

[Ref.: #30009]	Motility	yes
[Ref.: #120533]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.217

[Ref.: #30009]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5911]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #5911]

Culture medium growth

[Ref.: #5911]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5911]

Culture medium composition

expand / minimize

[Ref.: #38326]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38326]

Culture medium growth

[Ref.: #38326]

Culture medium composition

expand / minimize

[Ref.: #120533]

Culture medium

CIP Medium 72

[Ref.: #120533]

Culture medium growth

[Ref.: #120533]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5911]		growth	30 °C
[Ref.: #30009]		optimum	35 °C
[Ref.: #38326]		growth	30 °C
[Ref.: #120533]		growth	22-55 °C
[Ref.: #120533]	no	growth	10 °C

[Ref.: #5911]	Temperature range	mesophilic
[Ref.: #30009]	Temperature range	mesophilic
[Ref.: #38326]	Temperature range	mesophilic
[Ref.: #120533]	Temperature range	psychrophilic

		pH
[Ref.: #30009]	optimum	7
[Ref.: #30009]	growth	5.5-10.75
[Ref.: #120533]	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30009]	Oxygen tolerance	aerobe
[Ref.: #120533]	Oxygen tolerance	facultative anaerobe

[Ref.: #30009]	Ability of spore formation	yes
[Ref.: #120533]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #120533]	NaCl		growth	0-6	%
[Ref.: #120533]	NaCl	no	growth	8	%
[Ref.: #120533]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30009]	arabinose ChEBI	+	carbon source
[Ref.: #30009]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120533]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30009]	esculin ChEBI	+	hydrolysis
[Ref.: #120533]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	glycogen ChEBI	+	carbon source
[Ref.: #120533]	hippurate ChEBI	+	hydrolysis
[Ref.: #68371]	inulin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30009]	lactose ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	mannitol ChEBI	+	carbon source
[Ref.: #30009]	mannose ChEBI	+	carbon source
[Ref.: #30009]	melibiose ChEBI	+	carbon source
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	methyl D-glucoside ChEBI	+	carbon source
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30009]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #30009]	nitrate ChEBI	+	reduction
[Ref.: #120533]	nitrate ChEBI	+	reduction
[Ref.: #120533]	nitrate ChEBI	+	respiration
[Ref.: #120533]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30009]	raffinose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30009]	ribose ChEBI	+	carbon source
[Ref.: #30009]	salicin ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	sucrose ChEBI	+	carbon source
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30009]	trehalose ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30009]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120533]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #120533]		acetoin ChEBI	-
[Ref.: #120533]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120533]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120533]	amylase	+
[Ref.: #120533]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120533]	caseinase	-	3.4.21.50
[Ref.: #120533]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120533]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120533]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #120533]	gelatinase	+/-
[Ref.: #120533]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120533]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120533]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120533]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120533]	oxidase	+
[Ref.: #120533]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120533]	tween esterase	+
[Ref.: #120533]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120533]	11394	not determinedn.d.	+	-	+/-	+/-	+/-	+/-	-	-	+/-	+	+	+	+/-	-	-	-	-	+	+	+	+/-	+/-	+/-	+/-	+	+	+	+	+	+/-	+	+	+	+/-	+/-	+/-	+/-	-	+/-	+	-	-	-	+	-	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120533]	11490	-	+	+	+	-	+	-	-	-	+	+	+	+	+	-	+	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5911]	Sample type/isolated from	raw milk
[Ref.: #5911]	Geographic location (country and/or sea, region)	Wuustwezel
[Ref.: #5911]	Country	Belgium
[Ref.: #5911]	Country ISO 3 Code	BEL
[Ref.: #5911]	Continent	Europe

[Ref.: #120533]	Sample type/isolated from	Food, Raw milk
[Ref.: #120533]	Country	Belgium
[Ref.: #120533]	Country ISO 3 Code	BEL
[Ref.: #120533]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Host	#Mammals	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY257868 (>99% sequence identity) for Paenibacillus ihbetae from Microbeatlas

Aquatic Samples	111
Soil Samples	443
Animal Samples	206
Plant Samples	393
Total Samples	1153

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5911]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120533]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5911]	Paenibacillus lactis strain MB 1871 16S ribosomal RNA gene, partial sequence	AY257868	1508	ENA	228574 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paenibacillus lactis DSM 15596	GCA_017873605	contig	GenBank	228574 tax ID	*
[Ref.: #66792]	Paenibacillus lactis strain DSM 15596	228574.6	wgs	PATRIC	228574 tax ID	*
[Ref.: #66792]	Paenibacillus lactis DSM 15596	2913370050	draft	JGI IMG/M	228574 tax ID	*

[Ref.: #5911]	GC-content	51.6 mol%
[Ref.: #30009]	GC-content	51.65 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paenibacillus lactis strain DSM 15596 PATRIC: 228574.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus lactis strain DSM 15596 PATRIC: 228574.6
[Ref.: #69480]	motile	yes	93.136	yes
[Ref.: #69480]	gram-positive	yes	85.355	no
[Ref.: #69480]	anaerobic	no	98.025	no
[Ref.: #69480]	aerobic	yes	86.329	no
[Ref.: #69480]	halophile	no	84.062	no
[Ref.: #69480]	spore-forming	yes	96.822	yes
[Ref.: #69480]	glucose-util	yes	89.981	yes
[Ref.: #69480]	flagellated	yes	77.1	no
[Ref.: #69480]	thermophile	no	99.101	yes
[Ref.: #69480]	glucose-ferment	no	87.597	no

Availability in culture collections External links

[Ref.: #5911]

Culture collection no.

DSM 15596, CIP 108827, LMG 21940

[Ref.: #80791]

SI-ID 97185

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus ihbetae sp. nov., a cold-adapted antimicrobial producing bacterium isolated from high altitude Suraj Tal Lake in the Indian trans-Himalayas.	Kiran S, Swarnkar MK, Mayilraj S, Tewari R, Gulati A	Syst Appl Microbiol	10.1016/j.syapm.2017.07.005	2017	*
Phylogeny	Paenibacillus solani sp. nov., isolated from potato rhizosphere soil.	Liu B, Liu GH, Sengonca C, Schumann P, Lan JL, Chen DJ, Cui WD, Lin NQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001378	2016	*
Phylogeny	Paenibacillus zeae sp. nov., isolated from maize (Zea mays L.) seeds.	Liu Y, Zhai L, Wang R, Zhao R, Zhang X, Chen C, Cao Y, Cao Y, Xu T, Ge Y, Zhao J, Cheng C	Int J Syst Evol Microbiol	10.1099/ijsem.0.000608	2015	*
Phylogeny	Paenibacillus qingshengii sp. nov., isolated from a lead-zinc tailing.	Chen L, Wang L, Sheng XF	Int J Syst Evol Microbiol	10.1099/ijs.0.000232	2015	*
Phylogeny	Paenibacillus lemnae sp. nov., an endophytic bacterium of duckweed (Lemna aequinoctialis).	Kittiwongwattana C, Thawai C	Int J Syst Evol Microbiol	10.1099/ijs.0.067876-0	2014	*
Phylogeny	Paenibacillus lactis sp. nov., isolated from raw and heat-treated milk.	Scheldeman P, Goossens K, Rodriguez-Diaz M, Pil A, Goris J, Herman L, De Vos P, Logan NA, Heyndrickx M	Int J Syst Evol Microbiol	10.1099/ijs.0.02822-0	2004	*

References

#5911

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15596

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30009

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26373 (see below)

#38326 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80791

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID97185.1 )

#120533 Collection of Institut Pasteur ; Curators of the CIP; CIP 108827

#26373

IJSEM 885 2004 ( DOI 10.1099/ijs.0.02822-0 , PubMed 15143040 )

* These data were automatically processed and therefore are not curated

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Paenibacillus lactis strain DSM 15596

Paenibacillus lactis strain DSM 15596

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