Name: Brevibacillus choshinensis HPD 52
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 11423

Type strain:

Species: Brevibacillus choshinensis

Strain Designation: HPD 52, HPD52

Culture col. no.: DSM 8552, ATCC 51359, CIP 103838, IFO 15518, JCM 8505, NBRC 15518, CCUG 51061, NRRL B-23247, LMG 15968, CGMCC 1.3099, KCTC 3836, NCIMB 13345

Strain history: CIP <- 1993, H. Takagi, Higeta Shoyu Co., Chiba, Japan: strain HPD52

NCBI tax ID(s): 54911 (species)

SI-ID 8295

LMG 15968, ATCC 51359, CIP 103838, DSM 8552, IFO 15518, JCM 8505, NCIMB 13345, CCM 4534, NBRC 15518, BCRC 15920, CCRC 15920, KCTC 3836, KACC 10922, NRRL B-23032, NRRL B-23247, CCUG 51061

Special Collections

Other strains from Brevibacillus choshinensis

Section

Brevibacillus choshinensis HPD 52 is an obligate aerobe, spore-forming, Gram-positive bacterium that was isolated from soil.

Gram-positive
motile
rod-shaped
spore-forming
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Brevibacillus*
	Species	**Brevibacillus choshinensis**
	Full Scientific Name (LPSN)	Brevibacillus choshinensis (Takagi et al. 1993) Shida et al. 1996

Synonym

Bacillus choshinensis

Information on morphological and physiological properties Morphology

[Ref.: #118257]	Gram stain	positive

[Ref.: #118257]	Cell shape	rod-shaped

[Ref.: #118257]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3294]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #3294]

Culture medium growth

[Ref.: #3294]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3294]

Culture medium composition

expand / minimize

[Ref.: #39860]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #39860]

Culture medium growth

[Ref.: #39860]

Culture medium composition

expand / minimize

[Ref.: #118257]

Culture medium

CIP Medium 3

[Ref.: #118257]

Culture medium growth

[Ref.: #118257]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3294]		growth	30 °C
[Ref.: #39860]		growth	30 °C
[Ref.: #59096]		growth	30-37 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #118257]		growth	18-37 °C
[Ref.: #118257]	no	growth	10 °C
[Ref.: #118257]	no	growth	45 °C
[Ref.: #118257]	no	growth	55 °C

	pH	Kind of pH		pH
[Ref.: #118257]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118257]

Oxygen tolerance

obligate aerobe

[Ref.: #118257]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %91.734
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

[Ref.: #3294]

Name of produced compound

protein

	Salt	Tested relation		Salt conc.
[Ref.: #118257]	NaCl		growth	0-2	%
[Ref.: #118257]	NaCl	no	growth	4	%
[Ref.: #118257]	NaCl	no	growth	6	%
[Ref.: #118257]	NaCl	no	growth	8	%
[Ref.: #118257]	NaCl	no	growth	10	%

[Ref.: #67770]	Observation	Production of A large amount of protein
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #118257]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #118257]	nitrate ChEBI	-	reduction
[Ref.: #118257]	nitrate ChEBI	-	respiration
[Ref.: #118257]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #118257]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #118257]	amylase	-
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #118257]	caseinase	-	3.4.21.50
[Ref.: #118257]	catalase	-	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #118257]	gelatinase	-
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #118257]	lecithinase	-
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #118257]	oxidase	+
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #118257]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #59096]

Fatty acid	Percentage	ECL
C15:0 ANTEISO	36.1	14.711
C15:0 ISO	31.6	14.621
C17:0 iso	10.2	16.629
C16:0	7.9	16
C17:0 anteiso	6.2	16.722
C16:0 iso	3.5	15.626
C14:0 ISO	1.9	13.618
C16:1 ω11c	1.4	15.757
C14:0	1.3	14

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #59096]	5843	-	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #59096]	3269	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	+	+	-	+	+	-	-	+	+	+	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #118257]	9419	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3294]	Sample type/isolated from	soil

[Ref.: #59096]	Sample type/isolated from	Soil
[Ref.: #59096]	Geographic location (country and/or sea, region)	Chiba
[Ref.: #59096]	Country	Japan
[Ref.: #59096]	Country ISO 3 Code	JPN
[Ref.: #59096]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil

[Ref.: #118257]	Sample type/isolated from	Environment, Soil
[Ref.: #118257]	Geographic location (country and/or sea, region)	Chiba
[Ref.: #118257]	Country	Japan
[Ref.: #118257]	Country ISO 3 Code	JPN
[Ref.: #118257]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC379069 (>99% sequence identity) for Brevibacillus from Microbeatlas

Aquatic Samples	125
Soil Samples	355
Animal Samples	163
Plant Samples	89
Total Samples	732

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3294]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118257]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brevibacillus choshinensis gene for 16S rRNA, partial sequence, strain: JCM 8505	LC379069	1458	GenBank ENA	54911 tax ID
[Ref.: #20218]	Brevibacillus choshinensis clone Bchosh-1R 16S ribosomal RNA gene, and 16S-23S internal transcribed spacer, partial sequence	AF478095	257	GenBank ENA	54911 tax ID	*
[Ref.: #20218]	Brevibacillus choshinensis gene for 16S rRNA, partial sequence, strain:DSM 8552T	AB112713	1486	GenBank ENA	54911 tax ID	*
[Ref.: #20218]	Brevibacillus choshinensis DNA for 16S ribosomal RNA	D78459	1419	GenBank ENA	54911 tax ID	*
[Ref.: #20218]	Brevibacillus choshinensis gene for 16S rRNA, partial sequence, strain: NBRC 15518	AB680887	1461	GenBank ENA	54911 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brevibacillus choshinensis DSM 8552	GCA_001420695	scaffold	GenBank ENA	54911 tax ID
[Ref.: #66792]	Brevibacillus choshinensis strain DSM 8552	54911.3	wgs	PATRIC	54911 tax ID	*

[Ref.: #3294]

GC-content

48.2 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brevibacillus choshinensis strain DSM 8552 PATRIC: 54911.5
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brevibacillus choshinensis strain DSM 8552 PATRIC: 54911.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	58.659	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	91.3	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	91.734	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	75.161	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	91.787	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	78.668	no

Availability in culture collections External links

[Ref.: #3294]

Culture collection no.

DSM 8552, ATCC 51359, CIP 103838, IFO 15518, JCM 8505, NBRC 15518, CCUG 51061, NRRL B-23247, LMG 15968, CGMCC 1.3099, KCTC 3836, NCIMB 13345

[Ref.: #80657]	SI-ID 8295	*
[Ref.: #80658]	SI-ID 312200	*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Brevibacillus choshinensis HPD52T (DSM 8552), a Bacterial Host for Efficient Expression of Heterologous Proteins.	Wang JP, Liu B, Liu GH, Che JM, Chen Z, Chen M, Shi H	Genome Announc	10.1128/genomeA.01688-15	2016	*

References

#3294

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8552

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39860 ; Curators of the CIP;
#59096 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51061

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80657

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8295.1 )

#80658

#118257 Collection of Institut Pasteur ; Curators of the CIP; CIP 103838
* These data were automatically processed and therefore are not curated

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Brevibacillus choshinensis strain DSM 8552

Brevibacillus choshinensis strain DSM 8552

Change proposal

Change proposal