Name: Massilia tieshanensis TS3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11341

Type strain:

Species: Massilia tieshanensis

Strain Designation: TS3

Culture col. no.: DSM 25654, CCTCC AB 2010202, KACC 14940

Strain history: <- KACC <- G. Wang; TS3

NCBI tax ID(s): 796007 (species)

SI-ID 403759

DSM 25654

Section

Massilia tieshanensis TS3 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from subsurface soil of a metal mine with high concentrations of arsenic and other heavy metals.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Oxalobacteraceae*
	Genus	*Massilia*
	Species	**Massilia tieshanensis**
	Full Scientific Name (LPSN)	Massilia tieshanensis Du et al. 2012

Synonym

Telluria tieshanensis

Information on morphological and physiological properties Morphology

[Ref.: #30355]	Gram stain	negative

[Ref.: #30355]	Cell length	1.6 µm

[Ref.: #30355]	Cell width	0.45 µm

[Ref.: #30355]	Cell shape	rod-shaped

[Ref.: #30355]	Motility	yes

[Ref.: #18081]

Incubation period

1-2 days

[Ref.: #30355]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18081]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #18081]

Culture medium growth

[Ref.: #18081]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18081]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #18081]	growth	30 °C
[Ref.: #30355]	growth	10-40 °C
[Ref.: #30355]	optimum	28 °C

[Ref.: #18081]	Temperature range	mesophilic
[Ref.: #30355]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30355]			optimum	7
[Ref.: #30355]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30355]

Oxygen tolerance

aerobe

[Ref.: #30355]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30355]		NaCl		growth	1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30355]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30355]	arabinose ChEBI	+	carbon source
[Ref.: #30355]	arginine ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30355]	cellobiose ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30355]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #30355]	fructose ChEBI	+	carbon source
[Ref.: #30355]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #30355]	glucose ChEBI	+	carbon source
[Ref.: #30355]	glycogen ChEBI	+	carbon source
[Ref.: #30355]	histidine ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #30355]	lactate ChEBI	+	carbon source
[Ref.: #30355]	lactose ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #30355]	maltose ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #30355]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #30355]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #30355]	proline ChEBI	+	carbon source
[Ref.: #30355]	pyruvate ChEBI	+	carbon source
[Ref.: #30355]	raffinose ChEBI	+	carbon source
[Ref.: #30355]	sucrose ChEBI	+	carbon source
[Ref.: #30355]	threonine ChEBI	+	carbon source
[Ref.: #30355]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30355]	valerate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #30355]	acid phosphatase	+	3.1.3.2
[Ref.: #30355]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #18081]	catalase	+	1.11.1.6
[Ref.: #30355]	catalase	+	1.11.1.6
[Ref.: #30355]	cytochrome oxidase	+	1.9.3.1
[Ref.: #18081]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #30355]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #18081]	23908	+	-	-	-	-	+	+	-	+	+	+	-	-	+	+	-	-	+	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18081]	Sample type/isolated from	subsurface soil of a metal mine with high concentrations of arsenic and other heavy metals
[Ref.: #18081]	Geographic location (country and/or sea, region)	Hubei Province, Tieshan District, Daye City (30° 12' N 114° 53' E)
[Ref.: #18081]	Country	China
[Ref.: #18081]	Country ISO 3 Code	CHN
[Ref.: #18081]	Continent	Asia
[Ref.: #18081]	Geographic location	30.2000°/114.8830°
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Heavy metal
#Engineered	#Other	#Mine
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18081]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18081]	Massilia tieshanensis strain TS3 16S ribosomal RNA gene, partial sequence	HM130516	1452	ENA	796007 tax ID

[Ref.: #18081]	GC-content	65.9 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30355]	GC-content	65.9 mol%

Availability in culture collections External links

[Ref.: #18081]

Culture collection no.

DSM 25654, CCTCC AB 2010202, KACC 14940

[Ref.: #80574]

SI-ID 403759

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Massiliapinisoli sp. nov., isolated from forest soil.	Altankhuu K, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001249	2016	*
Phylogeny	Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing.	Feng GD, Yang SZ, Li HP, Zhu HH	Int J Syst Evol Microbiol	10.1099/ijsem.0.000670	2015	*
Phylogeny	Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia.	Singh H, Du J, Won K, Yang JE, Yin C, Kook M, Yi TH	Int J Syst Evol Microbiol	10.1099/ijsem.0.000477	2015	*
Phylogeny	Massilia tieshanensis sp. nov., isolated from mining soil.	Du Y, Yu X, Wang G	Int J Syst Evol Microbiol	10.1099/ijs.0.034306-0	2011	*

References

#18081

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25654

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30355

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26695 (see below)

#68369

Automatically annotated from API 20NE .

#80574

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403759.1 )

#26695

IJSEM 2356 2012 ( DOI 10.1099/ijs.0.034306-0 , PubMed 22140160 )

* These data were automatically processed and therefore are not curated

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