Massilia tieshanensis (DSM 25654, CCTCC AB 2010202, KACC 14940)

Name: Massilia tieshanensis (DSM 25654, CCTCC AB 2010202, KACC 14940)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11341

Type strain

Strain Designation TS3

Culture col. no. DSM 25654 CCTCC AB 2010202 KACC 14940 TS3

NCBI tax ID(s) 796007

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Massilia tieshanensis TS3 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from subsurface soil of a metal mine with high concentrations of arsenic and other heavy metals.

Gram-negative motile rod-shaped aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 403759

DSM 25654

@ref 20215

Last LPSN update 2025-12-16 09:49:34

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Oxalobacteraceae

Genus Massilia

Species Massilia tieshanensis

Full scientific name Massilia tieshanensis Du et al. 2012

Synonyms (1)

Telluria tieshanensis

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
30355	negative	1.6 µm	0.45 µm	rod-shaped

Colony morphology

18081

Incubation period1-2 days

Pigmentation

30355

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
18081	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
18081	positive	growth	30	mesophilic
30355	positive	growth	10-40
30355	positive	optimum	28	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
30355	positive	optimum	7
30355	positive	growth	05-09	alkaliphile

Physiology and metabolism

Oxygen tolerance

30355

Oxygen toleranceaerobe

Spore formation

30355

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30355	NaCl	positive	growth	1 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30355	37054 ChEBI	3-hydroxybutyrate	+	carbon source
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
30355	22599 ChEBI	arabinose	+	carbon source
30355	29016 ChEBI	arginine	+	carbon source
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
30355	17057 ChEBI	cellobiose	+	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
30355	4853 ChEBI	esculin	+	hydrolysis
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
30355	28757 ChEBI	fructose	+	carbon source
30355	28260 ChEBI	galactose	+	carbon source
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
30355	17234 ChEBI	glucose	+	carbon source
30355	28087 ChEBI	glycogen	+	carbon source
30355	27570 ChEBI	histidine	+	carbon source
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
30355	24996 ChEBI	lactate	+	carbon source
30355	17716 ChEBI	lactose	+	carbon source
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
30355	17306 ChEBI	maltose	+	carbon source
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
30355	37684 ChEBI	mannose	+	carbon source
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
30355	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
30355	26271 ChEBI	proline	+	carbon source
30355	15361 ChEBI	pyruvate	+	carbon source
30355	16634 ChEBI	raffinose	+	carbon source
30355	17992 ChEBI	sucrose	+	carbon source
30355	26986 ChEBI	threonine	+	carbon source
30355	27082 ChEBI	trehalose	+	carbon source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
30355	31011 ChEBI	valerate	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
30355	acid phosphatase	+	3.1.3.2
30355	alkaline phosphatase	+	3.1.3.1
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
18081	catalase	+	1.11.1.6
30355	catalase	+	1.11.1.6
30355	cytochrome oxidase	+	1.9.3.1
18081	cytochrome-c oxidase	+	1.9.3.1
30355	gelatinase	+
68369	gelatinase	+		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
18081	+	-	-	-	-	+	+	-	+	+	+	-	-	+	+	-	-	+	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Contamination	#Heavy metal
#Engineered	#Other	#Mine
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
18081	subsurface soil of a metal mine with high concentrations of arsenic and other heavy metals	Hubei Province, Tieshan District, Daye City (30° 12' N 114° 53' E)	China	CHN	Asia	30.2	114.883 30.2/114.883

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
18081	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
18081	Massilia tieshanensis strain TS3 16S ribosomal RNA gene, partial sequence	HM130516	1452		796007

GC content

@ref	GC-content (mol%)	Method
18081	65.9	high performance liquid chromatography (HPLC)
30355	65.9

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Massiliapinisoli sp. nov., isolated from forest soil.	Altankhuu K, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001249	2016
Phylogeny	Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing.	Feng GD, Yang SZ, Li HP, Zhu HH	Int J Syst Evol Microbiol	10.1099/ijsem.0.000670	2015
Phylogeny	Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia.	Singh H, Du J, Won K, Yang JE, Yin C, Kook M, Yi TH	Int J Syst Evol Microbiol	10.1099/ijsem.0.000477	2015
Phylogeny	Massilia tieshanensis sp. nov., isolated from mining soil.	Du Y, Yu X, Wang G	Int J Syst Evol Microbiol	10.1099/ijs.0.034306-0	2011

References

#18081	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25654
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#30355	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26695 (see below)
#68369	Automatically annotated from API 20NE .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Massilia tieshanensis (DSM 25654, CCTCC AB 2010202, KACC 14940)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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