Strain identifier

BacDive ID: 11341

Type strain: Yes

Species: Massilia tieshanensis

Strain Designation: TS3

Strain history: <- KACC <- G. Wang; TS3

NCBI tax ID(s): 796007 (species)

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General

@ref: 18081

BacDive-ID: 11341

DSM-Number: 25654

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Massilia tieshanensis TS3 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from subsurface soil of a metal mine with high concentrations of arsenic and other heavy metals.

NCBI tax id

  • NCBI tax id: 796007
  • Matching level: species

strain history

  • @ref: 18081
  • history: <- KACC <- G. Wang; TS3

doi: 10.13145/bacdive11341.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Oxalobacteraceae
  • genus: Massilia
  • species: Massilia tieshanensis
  • full scientific name: Massilia tieshanensis Du et al. 2012

  • synonyms

    • @ref: 20215
    • synonym: Telluria tieshanensis

@ref: 18081

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Massilia

species: Massilia tieshanensis

full scientific name: Massilia tieshanensis Du et al. 2012

strain designation: TS3

type strain: yes

Morphology

cell morphology

  • @ref: 30355
  • gram stain: negative
  • cell length: 1.6 µm
  • cell width: 0.45 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 18081
  • incubation period: 1-2 days

pigmentation

  • @ref: 30355
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 18081
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18081positivegrowth30mesophilic
30355positivegrowth10-40
30355positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
30355positivegrowth05-09alkaliphile
30355positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30355
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30355
  • spore formation: no

halophily

  • @ref: 30355
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 1 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
30355370543-hydroxybutyrate+carbon source
3035522599arabinose+carbon source
3035529016arginine+carbon source
3035517057cellobiose+carbon source
3035528757fructose+carbon source
3035528260galactose+carbon source
3035517234glucose+carbon source
3035528087glycogen+carbon source
3035527570histidine+carbon source
3035524996lactate+carbon source
3035517716lactose+carbon source
3035517306maltose+carbon source
3035537684mannose+carbon source
3035526271proline+carbon source
3035515361pyruvate+carbon source
3035516634raffinose+carbon source
3035517992sucrose+carbon source
3035526986threonine+carbon source
3035527082trehalose+carbon source
3035531011valerate+carbon source
303554853esculin+hydrolysis
3035517632nitrate+reduction
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
18081catalase+1.11.1.6
18081cytochrome-c oxidase+1.9.3.1
30355acid phosphatase+3.1.3.2
30355alkaline phosphatase+3.1.3.1
30355catalase+1.11.1.6
30355gelatinase+
30355cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
18081+----++-+++--++--+--

Isolation, sampling and environmental information

isolation

  • @ref: 18081
  • sample type: subsurface soil of a metal mine with high concentrations of arsenic and other heavy metals
  • geographic location: Hubei Province, Tieshan District, Daye City (30° 12' N 114° 53' E)
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 30.2
  • longitude: 114.883

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination#Heavy metal
#Engineered#Other#Mine
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 18081
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18081
  • description: Massilia tieshanensis strain TS3 16S ribosomal RNA gene, partial sequence
  • accession: HM130516
  • length: 1452
  • database: ena
  • NCBI tax ID: 796007

GC content

@refGC-contentmethod
1808165.9high performance liquid chromatography (HPLC)
3035565.9

External links

@ref: 18081

culture collection no.: DSM 25654, CCTCC AB 2010202, KACC 14940

straininfo link

  • @ref: 80574
  • straininfo: 403759

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22140160Massilia tieshanensis sp. nov., isolated from mining soil.Du Y, Yu X, Wang GInt J Syst Evol Microbiol10.1099/ijs.0.034306-02011Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, *Mining, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analysisGenetics
Phylogeny26220552Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia.Singh H, Du J, Won K, Yang JE, Yin C, Kook M, Yi THInt J Syst Evol Microbiol10.1099/ijsem.0.0004772015Agriculture, Bacterial Typing Techniques, Base Composition, Brassica, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phosphatidylethanolamines, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryGenetics
Phylogeny26449383Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing.Feng GD, Yang SZ, Li HP, Zhu HHInt J Syst Evol Microbiol10.1099/ijsem.0.0006702015Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Disulfides/metabolism, Fatty Acids/chemistry, *Mining, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tungsten, Ubiquinone/chemistryGenetics
Phylogeny27334790Massiliapinisoli sp. nov., isolated from forest soil.Altankhuu K, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0012492016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18081Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25654)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25654
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30355Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2669528776041
68369Automatically annotated from API 20NE
80574Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403759.1StrainInfo: A central database for resolving microbial strain identifiers