Name: Neptunomonas naphthovorans NAG-2N-126
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11281

Type strain:

Species: Neptunomonas naphthovorans

Strain Designation: NAG-2N-126

Culture col. no.: DSM 16183, ATCC 700637, CIP 106451, NBRC 101991

Strain history: CIP <- 2000, B.P. Hedlund, Dept. Microbiol., Univ. Washington, Seattle, USA: strain NAG-2N-126

NCBI tax ID(s): 75688 (species)

SI-ID 46339

ATCC 700637, CIP 106451, CECT 7132, NBRC 101991, DSM 16183

Section

Neptunomonas naphthovorans NAG-2N-126 is a psychrophilic, Gram-negative, motile bacterium that was isolated from coal tar creosote-contaminated marine sediment from a depth of 15.5 m.

Gram-negative
motile
rod-shaped
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Oceanospirillaceae*
	Genus	*Neptunomonas*
	Species	**Neptunomonas naphthovorans**
	Full Scientific Name (LPSN)	Neptunomonas naphthovorans Hedlund et al. 1999

Information on morphological and physiological properties Morphology

[Ref.: #121234]	Gram stain	negative

[Ref.: #121234]	Cell shape	rod-shaped

[Ref.: #121234]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6314]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6314]

Culture medium growth

[Ref.: #6314]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6314]

Culture medium composition

expand / minimize

[Ref.: #34639]

Culture medium

Marine agar (MA)

[Ref.: #34639]

Culture medium growth

[Ref.: #34639]

Culture medium composition

expand / minimize

[Ref.: #121234]

Culture medium

CIP Medium 13

[Ref.: #121234]

Culture medium growth

[Ref.: #121234]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6314]		growth	20 °C
[Ref.: #34639]		growth	25 °C
[Ref.: #121234]		growth	22-30 °C
[Ref.: #121234]	no	growth	5 °C
[Ref.: #121234]	no	growth	15 °C
[Ref.: #121234]	no	growth	37 °C
[Ref.: #121234]	no	growth	41 °C

[Ref.: #6314]	Temperature range	psychrophilic
[Ref.: #34639]	Temperature range	mesophilic
[Ref.: #121234]	Temperature range	psychrophilic
[Ref.: #121234]	Temperature range	mesophilic
[Ref.: #121234]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121234]	esculin ChEBI	-	hydrolysis
[Ref.: #121234]	nitrate ChEBI	-	reduction
[Ref.: #121234]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #121234]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #121234]		acetoin ChEBI	-
[Ref.: #121234]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121234]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121234]	amylase	-
[Ref.: #121234]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121234]	caseinase	-	3.4.21.50
[Ref.: #121234]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121234]	DNase	-
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121234]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121234]	gelatinase	-
[Ref.: #121234]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121234]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121234]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121234]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121234]	oxidase	+
[Ref.: #121234]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #121234]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121234]	tryptophan deaminase	-
[Ref.: #121234]	tween esterase	-
[Ref.: #121234]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121234]	9513	-	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6314]	Sample type/isolated from	coal tar creosote-contaminated marine sediment from a depth of 15.5 m
[Ref.: #6314]	Geographic location (country and/or sea, region)	Washington, Puget Sound, Eagle Harbor
[Ref.: #6314]	Country	USA
[Ref.: #6314]	Country ISO 3 Code	USA
[Ref.: #6314]	Continent	North America

[Ref.: #121234]	Sample type/isolated from	Environment, Creosote contaminated marine sediment
[Ref.: #121234]	Geographic location (country and/or sea, region)	Washington
[Ref.: #121234]	Country	United States of America
[Ref.: #121234]	Country ISO 3 Code	USA
[Ref.: #121234]	Continent	North America
[Ref.: #121234]	Isolation date	1993
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	-
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Geologic
#Environmental	#Terrestrial	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF053734 (>99% sequence identity) for Neptunomonas naphthovorans from Microbeatlas

Aquatic Samples	3926
Soil Samples	198
Animal Samples	667
Plant Samples	23
Total Samples	4814

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6314]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121234]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6314]	Neptunomonas naphthovorans 16S ribosomal RNA gene, partial sequence	AF053734	1466	ENA	75688 tax ID
[Ref.: #20218]	Neptunomonas naphthovorans gene for 16S rRNA, partial sequence, strain: NBRC 101991	AB681650	1468	ENA	75688 tax ID	*

Availability in culture collections External links

[Ref.: #6314]

Culture collection no.

DSM 16183, ATCC 700637, CIP 106451, NBRC 101991

[Ref.: #80514]

SI-ID 46339

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neptunomonas phycophila sp. nov. isolated from a culture of Symbiodinium sp., a dinoflagellate symbiont of the sea anemone Aiptasia tagetes.	Frommlet J, Guimaraes B, Sousa L, Serodio J, Alves A	Int J Syst Evol Microbiol	10.1099/ijs.0.000039	2015	*
Phylogeny	Neptunomonas acidivorans sp. nov., isolated from sediment, and emended description of the genus Neptunomonas.	Yang SH, Seo HS, Lee JH, Kim SJ, Kwon KK	Int J Syst Evol Microbiol	10.1099/ijs.0.064253-0	2014	*
Phylogeny	Corallomonas stylophorae gen. nov., sp. nov., a halophilic bacterium isolated from the reef-building coral Stylophora pistillata.	Chen MH, Sheu SY, Chen CA, Wang JT, Chen WM	Int J Syst Evol Microbiol	10.1099/ijs.0.043208-0	2012	*
Phylogeny	Neptunomonas antarctica sp. nov., isolated from marine sediment.	Zhang XY, Zhang YJ, Yu Y, Li HJ, Gao ZM, Chen XL, Chen B, Zhang YZ	Int J Syst Evol Microbiol	10.1099/ijs.0.017756-0	2009	*
Phylogeny	Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov.	Hedlund BP, Geiselbrecht AD, Bair TJ, Staley JT	Appl Environ Microbiol	10.1128/AEM.65.1.251-259.1999	1999	*

References

#6314

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16183

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34639 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80514

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46339.1 )

#121234 Collection of Institut Pasteur ; Curators of the CIP; CIP 106451
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Neptunomonas naphthovorans in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF