Strain identifier

BacDive ID: 11281

Type strain: Yes

Species: Neptunomonas naphthovorans

Strain Designation: NAG-2N-126

Strain history: CIP <- 2000, B.P. Hedlund, Dept. Microbiol., Univ. Washington, Seattle, USA: strain NAG-2N-126

NCBI tax ID(s): 75688 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6314

BacDive-ID: 11281

DSM-Number: 16183

keywords: 16S sequence, Bacteria, psychrophilic, Gram-negative, motile, rod-shaped

description: Neptunomonas naphthovorans NAG-2N-126 is a psychrophilic, Gram-negative, motile bacterium that was isolated from coal tar creosote-contaminated marine sediment from a depth of 15.5 m.

NCBI tax id

  • NCBI tax id: 75688
  • Matching level: species

strain history

@refhistory
6314<- ATCC <- B. P. Hedlund, Univ. of Washington, Seattle, USA <- A. D. Geiselbrecht; NAG-2N-126
121234CIP <- 2000, B.P. Hedlund, Dept. Microbiol., Univ. Washington, Seattle, USA: strain NAG-2N-126

doi: 10.13145/bacdive11281.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Oceanospirillaceae
  • genus: Neptunomonas
  • species: Neptunomonas naphthovorans
  • full scientific name: Neptunomonas naphthovorans Hedlund et al. 1999

@ref: 6314

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Oceanospirillaceae

genus: Neptunomonas

species: Neptunomonas naphthovorans

full scientific name: Neptunomonas naphthovorans Hedlund et al. 1999

strain designation: NAG-2N-126

type strain: yes

Morphology

cell morphology

  • @ref: 121234
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6314BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
34639Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
121234CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6314positivegrowth20psychrophilic
34639positivegrowth25mesophilic
121234positivegrowth22-30
121234nogrowth5psychrophilic
121234nogrowth15psychrophilic
121234nogrowth37mesophilic
121234nogrowth41thermophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1212344853esculin-hydrolysis
12123417632nitrate-reduction
12123416301nitrite-reduction

metabolite production

  • @ref: 121234
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12123415688acetoin-
12123417234glucose-

enzymes

@refvalueactivityec
121234oxidase+
121234beta-galactosidase-3.2.1.23
121234alcohol dehydrogenase-1.1.1.1
121234gelatinase-
121234amylase-
121234DNase-
121234caseinase-3.4.21.50
121234catalase+1.11.1.6
121234tween esterase-
121234gamma-glutamyltransferase-2.3.2.2
121234lecithinase-
121234lipase-
121234lysine decarboxylase-4.1.1.18
121234ornithine decarboxylase-4.1.1.17
121234phenylalanine ammonia-lyase-4.3.1.24
121234protease-
121234tryptophan deaminase-
121234urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121234-+---+----+---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6314coal tar creosote-contaminated marine sediment from a depth of 15.5 mWashington, Puget Sound, Eagle HarborUSAUSANorth America
121234Environment, Creosote contaminated marine sedimentWashingtonUnited States of AmericaUSANorth America1993

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Geologic
#Environmental#Terrestrial#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_16916.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_240;96_1716;97_2055;98_2524;99_16916&stattab=map
  • Last taxonomy: Neptunomonas naphthovorans
  • 16S sequence: AF053734
  • Sequence Identity:
  • Total samples: 4814
  • soil counts: 198
  • aquatic counts: 3926
  • animal counts: 667
  • plant counts: 23

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
63141Risk group (German classification)
1212341Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Neptunomonas naphthovorans gene for 16S rRNA, partial sequence, strain: NBRC 101991AB6816501468ena75688
6314Neptunomonas naphthovorans 16S ribosomal RNA gene, partial sequenceAF0537341466ena75688

External links

@ref: 6314

culture collection no.: DSM 16183, ATCC 700637, CIP 106451, NBRC 101991

straininfo link

  • @ref: 80514
  • straininfo: 46339

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9872786Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov.Hedlund BP, Geiselbrecht AD, Bair TJ, Staley JTAppl Environ Microbiol10.1128/AEM.65.1.251-259.19991999Bacteria/genetics/isolation & purification/*metabolism, Base Sequence, Biodegradation, Environmental, DNA Primers/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Dioxygenases, Genes, Bacterial, Genotype, Microscopy, Electron, Molecular Sequence Data, Multienzyme Complexes/genetics, Oxygenases/genetics, Phenotype, Phylogeny, Polycyclic Aromatic Hydrocarbons/*metabolism, Polymerase Chain Reaction, Water Microbiology, Water Pollutants, Chemical/*metabolismMetabolism
Phylogeny19801396Neptunomonas antarctica sp. nov., isolated from marine sediment.Zhang XY, Zhang YJ, Yu Y, Li HJ, Gao ZM, Chen XL, Chen B, Zhang YZInt J Syst Evol Microbiol10.1099/ijs.0.017756-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Geologic Sediments/*microbiology, Molecular Sequence Data, Oceanospirillaceae/*classification/genetics/*isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny22685102Corallomonas stylophorae gen. nov., sp. nov., a halophilic bacterium isolated from the reef-building coral Stylophora pistillata.Chen MH, Sheu SY, Chen CA, Wang JT, Chen WMInt J Syst Evol Microbiol10.1099/ijs.0.043208-02012Animals, Anthozoa/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Oceanospirillaceae/*classification/genetics/isolation & purification, Phospholipids/analysis, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TaiwanGenetics
Phylogeny25085876Neptunomonas acidivorans sp. nov., isolated from sediment, and emended description of the genus Neptunomonas.Yang SH, Seo HS, Lee JH, Kim SJ, Kwon KKInt J Syst Evol Microbiol10.1099/ijs.0.064253-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Molecular Sequence Data, Oceanospirillaceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryGenetics
Phylogeny25563909Neptunomonas phycophila sp. nov. isolated from a culture of Symbiodinium sp., a dinoflagellate symbiont of the sea anemone Aiptasia tagetes.Frommlet J, Guimaraes B, Sousa L, Serodio J, Alves AInt J Syst Evol Microbiol10.1099/ijs.0.0000392015Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Dinoflagellida/*microbiology, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Oceanospirillaceae/*classification/genetics/isolation & purification, *Phylogeny, Puerto Rico, RNA, Ribosomal, 16S/genetics, Sea Anemones/*microbiology, Sequence Analysis, DNA, Symbiosis, Ubiquinone/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6314Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16183)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16183
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34639Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18583
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80514Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46339.1StrainInfo: A central database for resolving microbial strain identifiers
121234Curators of the CIPCollection of Institut Pasteur (CIP 106451)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106451