Name: Thermobifida cellulosilytica TB100
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11260

Type strain:

Species: Thermobifida cellulosilytica

Strain Designation: TB100

Culture col. no.: DSM 44535, JCM 11767, NCAIM B 01997, NCAIM B.01997

Strain history: DSM 44535 <-- K. Márialigeti TB100.

NCBI tax ID(s): 665004 (strain), 144786 (species)

SI-ID 101002

DSM 44535, JCM 11767, NCAIM B.01997

Section

Thermobifida cellulosilytica TB100 is a thermophilic bacterium that was isolated from overheated manure compost.

thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Nocardiopsidaceae*
	Genus	*Thermobifida*
	Species	**Thermobifida cellulosilytica**
	Full Scientific Name (LPSN)	Thermobifida cellulosilytica corrig. Kukolya et al. 2002

Synonym

Thermobifida cellulolytica

Information on morphological and physiological properties Morphology

Multimedia content

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Caption:

Medium 65 45°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

CYC-MEDIUM (modified following Cross and Attwell,1973) (DSMZ Medium 550)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #11841]			growth	45 °C
[Ref.: #67770]			growth	45 °C

[Ref.: #11841]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

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Observation

quinones: MK-10(H₈), MK-10(H₆), MK-10(H₄)

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11841]	Sample type/isolated from	overheated manure compost
[Ref.: #11841]	Geographic location (country and/or sea, region)	Gödöll
[Ref.: #11841]	Country	Hungary
[Ref.: #11841]	Country ISO 3 Code	HUN
[Ref.: #11841]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Overheated manure compost
[Ref.: #67770]	Country	Hungary
[Ref.: #67770]	Country ISO 3 Code	HUN
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

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Global distribution of 16S sequence AJ298058 (>99% sequence identity) for Thermobifida cellulosilytica subclade from Microbeatlas

Aquatic Samples	267
Soil Samples	1772
Animal Samples	1008
Plant Samples	460
Total Samples	3507

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11841]	Thermobifida cellulolytica partial 16S rRNA gene, strain TB100	AJ298058	1495	ENA	665004 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Thermobifida cellulosilytica TB100	GCA_001517975	scaffold	GenBank	665004 tax ID
[Ref.: #66792]	Thermobifida cellulosilytica TB100	665004.4	wgs	PATRIC	665004 tax ID	*
[Ref.: #66792]	Thermobifida cellulosilytica TB100	2839655373	draft	JGI IMG/M	665004 tax ID	*
[Ref.: #66792]	Thermobifida cellulosilytica TB100	2503754044	draft	JGI IMG/M	665004 tax ID	*

[Ref.: #11841]	GC-content	70 mol%
[Ref.: #67770]	GC-content	70.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	72.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermobifida cellulosilytica TB100 PATRIC: 665004.4
[Ref.: #69480]	gram-positive	yes	88.46	no
[Ref.: #69480]	anaerobic	no	97.831	no
[Ref.: #69480]	halophile	no	58.01	no
[Ref.: #69480]	spore-forming	yes	85.207	no
[Ref.: #69480]	glucose-util	yes	84.297	no
[Ref.: #69480]	thermophile	no	90.844	no
[Ref.: #69480]	flagellated	no	98.195	no
[Ref.: #69480]	motile	no	94.825	no
[Ref.: #69480]	aerobic	yes	84.689	no
[Ref.: #69480]	glucose-ferment	no	91.965	no

Availability in culture collections External links

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Culture collection no.

DSM 44535, JCM 11767, NCAIM B 01997, NCAIM B.01997

[Ref.: #80493]

SI-ID 101002

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Structure-function analysis of two closely related cutinases from Thermobifida cellulosilytica.	Arnling Baath J, Novy V, Carneiro LV, Guebitz GM, Olsson L, Westh P, Ribitsch D	Biotechnol Bioeng	10.1002/bit.27984	2021	*
Pathogenicity	Bioremediation of lindane contaminated soil: Exploring the potential of actinobacterial strains.	Usmani Z, Kulp M, Lukk T	Chemosphere	10.1016/j.chemosphere.2021.130468	2021	*
Biotechnology	Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis.	Herrero Acero E, Ribitsch D, Dellacher A, Zitzenbacher S, Marold A, Steinkellner G, Gruber K, Schwab H, Guebitz GM	Biotechnol Bioeng	10.1002/bit.24930	2013	*
Phylogeny	Thermobifida cellulolytica sp. nov., a novel lignocellulose-decomposing actinomycete.	Kukolya J, Nagy I, Laday M, Toth E, Oravecz O, Marialigeti K, Hornok L	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1193	2002	*

References

#11841

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44535

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80493

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID101002.1 )

* These data were automatically processed and therefore are not curated

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