Nocardiopsis quinghaiensis (DSM 44739, CGMCC 4.3494, YIM 28A4)

Name: Nocardiopsis quinghaiensis (DSM 44739, CGMCC 4.3494, YIM 28A4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11232

Type strain

Culture col. no. DSM 44739 CGMCC 4.3494 YIM 28A4

NCBI tax ID(s) 464995

Special Collections DSMZ Wink compendium Literature strains

History DSMZ <- X. Cui, Yunnan Univ., Kunming; YIM 28A4

Links

Nocardiopsis quinghaiensis DSM 44739 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from alkaline soil samples.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 389430

CGMCC 4.3494,DSM 44739

@ref 20215

Last LPSN update 2026-05-29 08:27:31

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Nocardiopsidaceae

Genus Nocardiopsis

Species Nocardiopsis quinghaiensis

Full scientific name Nocardiopsis quinghaiensis Chen et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Motility	Confidence
32404	positive
125439	positive		94.538

Colony morphology

@ref	Colony color	Incubation period	Medium used
20108	Beige (1001)	10-14 days	ISP 2
20108		10-14 days	ISP 3
20108	Beige (1001)	10-14 days	ISP 4
20108		10-14 days	ISP 5
20108		10-14 days	ISP 6
20108		10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20108		ISP 2
20108		ISP 3
20108		ISP 4
20108		ISP 5
20108		ISP 6
20108		ISP 7

Pigmentation

32404

Productionno

Multimedia

@ref:	12053
multimedia content:	DSM_44739.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44739.jpg
caption:	Medium 514 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12053	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
20108	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20108	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20108	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20108	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20108	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20108	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
12053	CZAPEK PEPTONE AGAR (DSMZ Medium 83)	Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
12053	positive	growth	28
20108	positive	optimum	28
32404	positive	growth	10-37
32404	positive	optimum	28

Culture pH

@ref	Ability	Type	PH
32404	positive	optimum	7
32404	positive	growth	06-08

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
32404	aerobe
125439	facultative anaerobe	94.215

Spore formation

32404

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
32404	NaCl	positive	growth	0-10 %
32404	NaCl	positive	optimum	3 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32404	22599 ChEBI	arabinose	+	carbon source
32404	17057 ChEBI	cellobiose	+	carbon source
32404	16947 ChEBI	citrate	+	carbon source
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
32404	28757 ChEBI	fructose	+	carbon source
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
32404	17234 ChEBI	glucose	+	carbon source
32404	17754 ChEBI	glycerol	+	carbon source
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
32404	29864 ChEBI	mannitol	+	carbon source
32404	37684 ChEBI	mannose	+	carbon source
32404	17268 ChEBI	myo-inositol	+	carbon source
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
32404	16634 ChEBI	raffinose	+	carbon source
32404	33942 ChEBI	ribose	+	carbon source
32404	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
32404	27082 ChEBI	trehalose	+	carbon source
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
32404	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
20108	+	-	-	-	-	-	-	-	-	+	-	not determinedn.d.	+	-	-	-	+	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20108	not determinedn.d.	+	+	+	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Condition	#Alkaline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12053	alkaline soil samples	Qaidam Basin	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence EF597511 (>99% sequence identity) for Nocardiopsis from Microbeatlas

Aquatic Samples	0
Soil Samples	7
Animal Samples	14
Plant Samples	1
Total Samples	22

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12053	1	Risk group (German classification) According to TRBA 466
20108	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM863836v1 assembly for Nocardiopsis quinghaiensis YIM 28A4	scaffold	GCA_008638365	464995.3	2989480425	464995	64.35

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12053	Nocardiopsis quinghaiensis strain YIM 28A4 16S ribosomal RNA gene, partial sequence	EF597511	1384		464995

GC content

12053

GC-content (mol%)67.1

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM863836v1 assembly for Nocardiopsis quinghaiensis YIM 28A4
NCBI: GCA_008638365

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	94.22	no
125439	gram_stain	BacteriaNetⓘ	positive	94.54	no
125439	motility	BacteriaNetⓘ	no	87.64	no
125439	spore_formation	BacteriaNetⓘ	yes	36.85	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Culture-dependent and culture-independent characterization of bacterial community diversity in different types of sandy lands: the case of Minqin County, China.	Wei Y, Wang F, Gao J, Huang Y, Ren W, Sheng H.	BMC Microbiol	10.1186/s12866-021-02150-0	2021
Phylogeny	Nocardiopsis sinuspersici sp. nov., isolated from sandy rhizospheric soil.	Hamedi J, Mohammadipanah F, von Jan M, Potter G, Schumann P, Sproer C, Klenk HP, Kroppenstedt RM.	Int J Syst Evol Microbiol	10.1099/ijs.0.018366-0	2010
Phylogeny	Nocardiopsis arvandica sp. nov., isolated from sandy soil.	Hamedi J, Mohammadipanah F, Potter G, Sproer C, Schumann P, Goker M, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijs.0.022756-0	2010
Phylogeny	Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China.	Chen YG, Cui XL, Kroppenstedt RM, Stackebrandt E, Wen ML, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.65441-0	2008

References

#12053	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44739
#20108	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28628	IJSEM 699 2008 ( DOI 10.1099/ijs.0.65441-0 , PubMed 18319481 )
#32404	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28628
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Nocardiopsis quinghaiensis (DSM 44739, CGMCC 4.3494, YIM 28A4)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_008638365

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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