Nocardiopsis lucentensis (DSM 44048, ATCC 51300, IFO 15854)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	11462
  multimedia content:	DSM_44048.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44048.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
11462	ISP MEDIUM 2 WITH 5% NaCl (DSMZ Medium 636)		Medium recipe at MediaDive	Name: ISP MEDIUM 2 WITH 5% NaCl (DSMZ Medium 636) Composition: NaCl 50.0 g/l Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
11462	GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) Composition: NaCl 100.0 g/l Agar 15.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19623	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19623	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19623	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11462	ISP2 MEDIUM (DSMZ Medium 987)		Medium recipe at MediaDive	Name: ISP 2 MEDIUM (DSMZ Medium 987; with strain-specific modifications) Composition: NaCl 30.0 g/l Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water

Culture temp

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19623	22599 ChEBI	arabinose	+/-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19623	62968 ChEBI	cellulose	+/-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
19623	28757 ChEBI	fructose	+/-
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19623	17234 ChEBI	glucose	+/-
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19623	29864 ChEBI	mannitol	+/-
19623	17268 ChEBI	myo-inositol	+/-
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19623	16634 ChEBI	raffinose	+/-
19623	26546 ChEBI	rhamnose	+/-
19623	17992 ChEBI	sucrose	+/-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19623	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence X97888 (>99% sequence identity) for Nocardiopsis from Microbeatlas

Aquatic Samples	0
Soil Samples	0
Animal Samples	9
Plant Samples	1
Total Samples	10

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	SOAPdenovo v1.06 assembly for Nocardiopsis lucentensis DSM 44048	contig	GCA_000341125	1246460.3	2554235034	1246460	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	N.lucentensis 16S rRNA gene	X97888	1461		1246460
124043	Nocardiopsis lucentensis strain DSM 44048(T) 16S ribosomal RNA gene, partial sequence.	MZ666930	698		53441

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Next-generation biotechnology inspired by extremes : The potential of extremophile organisms for synthetic biology and for more efficient and sustainable biotechnology.	Zheng S, Shao M, Wang W, Chen GQ.	EMBO Rep	10.1038/s44319-025-00389-6	2025
	Inhibition and eradication of Pseudomonas aeruginosa biofilms by secondary metabolites of Nocardiopsis lucentensis EMB25.	Goel N, Ghosh M, Jain D, Sinha R, Khare SK.	RSC Med Chem	10.1039/d2md00439a	2023
	Salt-Adapted Microorganisms: A Promising Resource for Novel Anti-Cancer Drug Discovery.	Fang L, Xu L, Kader M, Ding T, Lu S, Wang D, Sharma AR, Zhang Z.	Mar Drugs	10.3390/md23080296	2025
Metabolism	Natural Product Potential of the Genus Nocardiopsis.	Ibrahim AH, Desoukey SY, Fouad MA, Kamel MS, Gulder TAM, Abdelmohsen UR.	Mar Drugs	10.3390/md16050147	2018
	Recent Achievements in Total Synthesis for Integral Structural Revisions of Marine Natural Products.	Ha MW, Kim J, Paek SM.	Mar Drugs	10.3390/md20030171	2022
	An Overview of Bioactive 1,3-Oxazole-Containing Alkaloids from Marine Organisms.	Chen J, Lv S, Liu J, Yu Y, Wang H, Zhang H.	Pharmaceuticals (Basel)	10.3390/ph14121274	2021
Enzymology	Bioprospecting potential of halogenases from Arctic marine actinomycetes.	Liao L, Chen R, Jiang M, Tian X, Liu H, Yu Y, Fan C, Chen B.	BMC Microbiol	10.1186/s12866-016-0662-2	2016
	New Insights on Biological Activities, Chemical Compositions, and Classifications of Marine Actinomycetes Antifouling Agents.	Morgan RN, Ali AA, Alshahrani MY, Alshahrani MY, Alshahrani MY, Aboshanab KM.	Microorganisms	10.3390/microorganisms11102444	2023
	Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine.	Corral P, Amoozegar MA, Ventosa A.	Mar Drugs	10.3390/md18010033	2019
	Computational Investigations on the Natural Small Molecule as an Inhibitor of Programmed Death Ligand 1 for Cancer Immunotherapy.	Kumar GS, Moustafa M, Sahoo AK, Maly P, Bharadwaj S.	Life (Basel)	10.3390/life12050659	2022
	Natural Products Research in China From 2015 to 2016.	Liu H, Zhu G, Fan Y, Du Y, Lan M, Xu Y, Zhu W.	Front Chem	10.3389/fchem.2018.00045	2018
	Biology of moderately halophilic aerobic bacteria.	Ventosa A, Nieto JJ, Oren A.	Microbiol Mol Biol Rev	10.1128/mmbr.62.2.504-544.1998	1998
Phylogeny	Distribution and phylogenetic analysis of family 19 chitinases in Actinobacteria.	Kawase T, Saito A, Sato T, Kanai R, Fujii T, Nikaidou N, Miyashita K, Watanabe T.	Appl Environ Microbiol	10.1128/aem.70.2.1135-1144.2004	2004
	Whole genome sequencing and functional analysis of a novel biofilm-eradicating strain Nocardiopsis lucentensis EMB25	Goel N, Zaidi S, Khare SK.	World J Microbiol Biotechnol.		2023
Genetics	Whole genome sequencing and functional analysis of a novel biofilm-eradicating strain Nocardiopsis lucentensis EMB25.	Goel N, Zaidi S, Khare SK.	World J Microbiol Biotechnol	10.1007/s11274-023-03738-6	2023
Metabolism	Nocardiopsis lucentensis and thiourea co-application mitigates arsenic stress through enhanced antioxidant metabolism and lignin accumulation in rice.	AbdElgawad H, Negi P, Zinta G, Mohammed AE, Alotaibi MO, Beemster G, Saleh AM, Srivastava AK.	Sci Total Environ	10.1016/j.scitotenv.2023.162295	2023
	An actinomycete strain of Nocardiopsis lucentensis reduces arsenic toxicity in barley and maize.	AbdElgawad H, Zinta G, Abuelsoud W, Hassan YM, Alkhalifah DHM, Hozzein WN, Zrieq R, Beemster GT, Schoenaers S.	J Hazard Mater	10.1016/j.jhazmat.2021.126055	2021
Phylogeny	The Potential of Phylogenetically Diverse Culturable Actinobacteria from Litopenaeus vannamei Pond Sediment as Extracellular Proteolytic and Lipolytic Enzyme Producers.	Ayuningrum D, Sabdaningsih A, Jati OE.	Trop Life Sci Res	10.21315/tlsr2022.33.3.10	2022
Enzymology	Isolation, characterization, and complete genome analysis of P1312, a thermostable bacteriophage that infects Thermobifida fusca.	Cheepudom J, Lee CC, Cai B, Meng M.	Front Microbiol	10.3389/fmicb.2015.00959	2015
Pathogenicity	Colorectal cancer treatment using bacteria: focus on molecular mechanisms.	Ebrahimzadeh S, Ahangari H, Soleimanian A, Hosseini K, Ebrahimi V, Ghasemnejad T, Soofiyani SR, Tarhriz V, Eyvazi S.	BMC Microbiol	10.1186/s12866-021-02274-3	2021
Metabolism	Production of a new glycolipid biosurfactant from marine Nocardiopsis lucentensis MSA04 in solid-state cultivation.	Kiran GS, Thomas TA, Selvin J.	Colloids Surf B Biointerfaces	10.1016/j.colsurfb.2010.01.028	2010
	Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment.	Sharma JK, Kumar N, Singh NP, Santal AR.	Front Plant Sci	10.3389/fpls.2023.1076876	2023
	Lucentamycins A-D, cytotoxic peptides from the marine-derived actinomycete Nocardiopsis lucentensis.	Cho JY, Williams PG, Kwon HC, Jensen PR, Fenical W.	J Nat Prod	10.1021/np070101b	2007
	Structure assignment of lucentamycin E and revision of the olefin geometries of the marine-derived lucentamycins.	Cha JW, Park JS, Sim T, Nam SJ, Kwon HC, Del Valle JR, Fenical W.	J Nat Prod	10.1021/np3003854	2012
Pathogenicity	Bacteria-Mediated Modulatory Strategies for Colorectal Cancer Treatment.	Mueller AL, Brockmueller A, Fahimi N, Ghotbi T, Hashemi S, Sadri S, Khorshidi N, Kunnumakkara AB, Shakibaei M.	Biomedicines	10.3390/biomedicines10040832	2022
Metabolism	Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived Actinokineospora and Rhodococcus spp.	Alhadrami HA, Thissera B, Hassan MHA, Behery FA, Ngwa CJ, Hassan HM, Pradel G, Abdelmohsen UR, Rateb ME.	Mar Drugs	10.3390/md19020109	2021
	Teichoic acids of the cell wall of Nocardiopsis listeri, Nocardiopsis lucentensis, and Nocardiopsis tregalosei.	Streshinskaya GM, Tul'skaya EM, Shashkov AS, Evtushenko LI, Taran VV, Naumova IB.	Biochemistry (Mosc)		1998
Phylogeny	The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.	Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E.	Int J Syst Bacteriol	10.1099/00207713-46-4-1088	1996
	Kocuria marina BS-15 a biosurfactant producing halophilic bacteria isolated from solar salt works in India.	Sarafin Y, Donio MB, Velmurugan S, Michaelbabu M, Citarasu T.	Saudi J Biol Sci	10.1016/j.sjbs.2014.01.001	2014
	Borrelidins C-E: New Antibacterial Macrolides from a Saltern-Derived Halophilic Nocardiopsis sp.	Kim J, Shin D, Kim SH, Park W, Shin Y, Kim WK, Lee SK, Oh KB, Shin J, Oh DC.	Mar Drugs	10.3390/md15060166	2017
	Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide.	Nouioui I, Boldt J, Zimmermann A, Makitrynskyy R, Potter G, Jando M, Doppner M, Kirstein S, Neumann-Schaal M, Gomez-Escribano JP, Nubel U, Mast Y.	Curr Res Microb Sci	10.1016/j.crmicr.2024.100290	2024
	Phylogenetic diversity of actinomycetes cultured from coastal multipond solar saltern in Tuticorin, India.	Jose PA, Jebakumar SR.	Aquat Biosyst	10.1186/2046-9063-8-23	2012
	Response Surface Methodology for Optimizing the Production of Biosurfactant by Candida tropicalis on Industrial Waste Substrates.	Almeida DG, Soares da Silva RC, Luna JM, Rufino RD, Santos VA, Sarubbo LA.	Front Microbiol	10.3389/fmicb.2017.00157	2017
	GapMind: Automated Annotation of Amino Acid Biosynthesis.	Price MN, Deutschbauer AM, Arkin AP.	mSystems	10.1128/msystems.00291-20	2020
	Advances in utilization of renewable substrates for biosurfactant production.	Makkar RS, Cameotra SS, Banat IM.	AMB Express	10.1186/2191-0855-1-5	2011
Phylogeny	Sequence-based analysis of secondary-metabolite biosynthesis in marine actinobacteria.	Gontang EA, Gaudencio SP, Fenical W, Jensen PR.	Appl Environ Microbiol	10.1128/aem.02852-09	2010
Pathogenicity	Synthesis, Structure Revision, and Cytotoxicity of Nocarbenzoxazole G.	Kim T, Lee SA, Noh T, Choi P, Choi SJ, Song BG, Kim Y, Park YT, Huh G, Kim YJ, Ham J	J Nat Prod	10.1021/acs.jnatprod.9b00072	2019
	Two new polyketides from Nocardiopsis lucentensis DSM 44048.	Sun MW, Guo ZX, Lu CH	Nat Prod Res	10.1080/14786419.2015.1104674	2015
Pathogenicity	Nocarbenzoxazoles A-G, Benzoxazoles Produced by Halophilic Nocardiopsis lucentensis DSM 44048.	Sun M, Zhang X, Hao H, Li W, Lu C	J Nat Prod	10.1021/acs.jnatprod.5b00031	2015
	Nocardiopsis eucommiae sp. nov., a novel endophytic actinomycete isolated from leaves of Eucommia ulmoides Oliv.	Mo P, Chen Y, Zou F, Zhou J, Zou W, Gao J.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005654	2022
Phylogeny	Nocardiopsis flavescens sp. nov., an actinomycete isolated from marine sediment.	Fang C, Zhang J, Pang H, Li Y, Xin Y, Zhang Y.	Int J Syst Evol Microbiol	10.1099/ijs.0.027987-0	2011
Phylogeny	Murinocardiopsis flavida gen. nov., sp. nov., an actinomycete isolated from indoor walls.	Kampfer P, Schafer J, Lodders N, Martin K.	Int J Syst Evol Microbiol	10.1099/ijs.0.015990-0	2010
Enzymology	Nocardiopsis aegyptia sp. nov., isolated from marine sediment.	Sabry SA, Ghanem NB, Abu-Ella GA, Schumann P, Stackebrandt E, Kroppenstedt RM.	Int J Syst Evol Microbiol	10.1099/ijs.0.02814-0	2004
Phylogeny	Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil.	Bouras N, Meklat A, Zitouni A, Mathieu F, Schumann P, Sproer C, Sabaou N, Klenk HP	Antonie Van Leeuwenhoek	10.1007/s10482-014-0329-7	2014
Phylogeny	Nocardiopsis fildesensis sp. nov., an actinomycete isolated from soil.	Xu S, Yan L, Zhang X, Wang C, Feng G, Li J	Int J Syst Evol Microbiol	10.1099/ijs.0.053595-0	2013