Rhodococcus rhodochrous (DSM 43269, ATCC 4273, CBS 197.57)

Name: Rhodococcus rhodochrous (DSM 43269, ATCC 4273, CBS 197.57)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10904

Type strain

Culture col. no. DSM 43269 ATCC 4273 CBS 197.57 CCM 3245 CIP 104623 18 more

NCBI tax ID(s) 1829

Special Collections DSMZ CCUG JCM CIP

History <- K. Kieslich, Schering 203 (Mycobacterium agreste) <- ATCC <- H.J. Conn <- P.H.H. Gray, 0-3 KCC A-0010 <-- ATCC 4273 <-- H. J. Conn <-- P. H. H. Gray 0-3. CIP <- 1995, NCIMB <- R.E. Gordon <- ATCC <- H.J. Conn, Mycobacterium agreste <- P.H.H. Gray: strain 0-3, Nocardia corallina

Links

Rhodococcus rhodochrous DSM 43269 is an obligate aerobe, Gram-positive, rod-shaped bacterium of the family Nocardiaceae.

Gram-positive rod-shaped obligate aerobe Bacteria

Name and taxonomic classification

SI-ID 92710

LMG 4077,ATCC 4273,CCM 3245,CECT 3046,IAM 12121,IFO 3338,IMET 7018,NCIB 9259,ATCC 21664,CCUG 18001,CIP 104623,NCIMB 9259,JCM 3010,CCRC 11075,VKM Ac-860,CBS 197.57,CECT 3091,NBRC 3338,NCCB 91001,LMD 91.1,BCRC 11075,NBIMCC 1359,CGMCC 4.1073,CGMCC 4.1480,DSM 43269

@ref 20215

Last LPSN update 2026-02-09 11:20:23

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Nocardiaceae

Genus Rhodococcus

Species Rhodococcus rhodochrous

Full scientific name Rhodococcus rhodochrous (Zopf 1891) Tsukamura 1974 (Approved Lists 1980)

Synonyms (2)

"Staphylococcus rhodochrous"
Rhodococcus roseus

BacDive ID	Other strains from **Rhodococcus rhodochrous** (27)	Type strain
10908	R. rhodochrous 372, KMRh, DSM 43241, ATCC 13808, CIP 104376, ... (type strain)
10560	R. rhodochrous DSM 43240, NCTC 8139, CIP 104649
10844	R. rhodochrous N814, DSM 43969
10875	R. rhodochrous N56, DSM 43956
10897	R. rhodochrous DSM 363, ATCC 4276, DSM 43202, JCM 20420, ...
10898	R. rhodochrous An117, DSM 6263, IMET 7497
10900	R. rhodochrous S5, DSM 6697
10901	R. rhodochrous OR, DSM 11097
10905	R. rhodochrous DSM 43198, NCIB 9701, CIP 104622, IMET 7278, ...
10909	R. rhodochrous DSM 43273, ATCC 9356, NRRL B-1306
10910	R. rhodochrous DSM 43274, ATCC 271, JCM 2156, BCRC 13391, ...
10913	R. rhodochrous N31 R312, W 21, DSM 43334
10916	R. rhodochrous N 1030, DSM 43986, IMV 347
10917	R. rhodochrous N27H, DSM 46022, CCM 278, IMET 7285, NCIB ...
10918	R. rhodochrous Gdf N31, Gordon W21, N31, Overbeck, DSM 46023, ...
10919	R. rhodochrous 40019, Breed He-11, Gdf R10, DSM 46044, ATCC ...
103565	R. rhodochrous STH00245(ZIMET), I-449, HKI 0245
131010	R. rhodochrous PY11, DSM 101666
131917	R. rhodochrous RPK 1, DSM 103064
136316	R. rhodochrous CIP 104635, PCM 909
136329	R. rhodochrous N454, CIP 104638, PCM 2216
139137	R. rhodochrous CIP 104496, ATCC 4004, IAM 12127, JCM 2158
139161	R. rhodochrous CIP 82.104, ATCC 184, NCTC 2682, NCTC 7510
144204	R. rhodochrous CCUG 23576, ATCC 25721
145853	R. rhodochrous CCUG 29792
163232	R. rhodochrous 1652, JCM 2157, ATCC 999, CIP 104495, IAM ...
169282	R. rhodochrous G38GP, DSM 111905

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
122228	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
10801	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
35702	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
122228	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
10801	positive	growth	28
35702	positive	growth	30
67770	positive	growth	28
122228	positive	growth	10-41
122228	negative	growth	45

Physiology and metabolism

Oxygen tolerance

122228

Oxygen toleranceobligate aerobe

Compound production

10801

Compoundrestriction endonuclease Rrh4372I

Halophily

@ref	Salt	Growth	Tested relation	Concentration
122228	NaCl	positive	growth	0-6 %
122228	NaCl		growth	8 %
122228	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-8(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
122228	16947 ChEBI	citrate	-	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
122228	4853 ChEBI	esculin	+	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
122228	17632 ChEBI	nitrate	-	builds gas from
122228	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
122228	16301 ChEBI	nitrite	+	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122228	35581 ChEBI	indole
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test	Methylred-test
122228	15688 ChEBI	acetoin		-
122228	17234 ChEBI	glucose			-
68369	35581 ChEBI	indole	-			from API 20NE

Enzymes

@ref	Value	Activity	Ec
122228	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122228	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
122228	beta-galactosidase	+/-	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122228	caseinase	-	3.4.21.50
122228	catalase	+	1.11.1.6
122228	DNase	-
122228	gamma-glutamyltransferase	-	2.3.2.2
122228	gelatinase	-
68369	gelatinase	-		from API 20NE
122228	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
122228	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
122228	ornithine decarboxylase	-	4.1.1.17
122228	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
122228	tryptophan deaminase	-
122228	tween esterase	+
122228	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
10801	-	-	-	-	-	+	+/-	-	-	-	-	-	-	-	-	+	+	-	-	-
122228	-	-	+	+	+	+	+	+	-	-	+	+	-	-	-	+	+	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
10801	+	-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	+/-	-	+/-	+	-	-	not determinedn.d.

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122228	not determinedn.d.	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	-	-	-	-	+	-	-	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
10801	yes, in single cases	1	Risk group (German classification) According to TRBA 466
122228		1	Risk group (French classification) According to TRBA 466

Sequence information

GC content

@ref	GC-content (mol%)	Method
67770	68.8	high performance liquid chromatography (HPLC)
67770	66	fluorimetric

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Whole-Genome Analysis of Mycobacterium neoaurum DSM 1381 and the Validation of Two Key Enzymes Affecting C22 Steroid Intermediates in Sterol Metabolism.	Zhang J, Zhang R, Song S, Su Z, Shi J, Cao H, Zhang B.	Int J Mol Sci	10.3390/ijms24076148	2023
	The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001.	Tian K, Meng F, Meng Q, Gao Y, Zhang L, Wang L, Wang Y, Li X, Huo H.	Int J Genomics	10.1155/2020/9369182	2020
Metabolism	Engineered 3-Ketosteroid 9alpha-Hydroxylases in Mycobacterium neoaurum: an Efficient Platform for Production of Steroid Drugs.	Liu HH, Xu LQ, Yao K, Xiong LB, Tao XY, Liu M, Wang FQ, Wei DZ.	Appl Environ Microbiol	10.1128/aem.02777-17	2018
Metabolism	Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate.	Shtratnikova VY, Sshelkunov MI, Fokina VV, Bragin EY, Shutov AA, Donova MV.	BMC Biotechnol	10.1186/s12896-021-00668-9	2021
	New Insights on Steroid Biotechnology.	Fernandez-Cabezon L, Galan B, Garcia JL.	Front Microbiol	10.3389/fmicb.2018.00958	2018
Metabolism	The essential function of genes for a hydratase and an aldehyde dehydrogenase for growth of Pseudomonas sp. strain Chol1 with the steroid compound cholate indicates an aldolytic reaction step for deacetylation of the side chain.	Holert J, Jagmann N, Philipp B.	J Bacteriol	10.1128/jb.00410-13	2013
Metabolism	Testosterone Degradative Pathway of Novosphingobium tardaugens.	Ibero J, Galan B, Diaz E, Garcia JL.	Genes (Basel)	10.3390/genes10110871	2019
	A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality.	van der Geize R, de Jong W, Hessels GI, Grommen AW, Jacobs AA, Dijkhuizen L.	Nucleic Acids Res	10.1093/nar/gkn811	2008
Metabolism	Actinobacterial acyl coenzyme A synthetases involved in steroid side-chain catabolism.	Casabon I, Swain K, Crowe AM, Eltis LD, Mohn WW.	J Bacteriol	10.1128/jb.01012-13	2014
Metabolism	Pathogen roid rage: cholesterol utilization by Mycobacterium tuberculosis.	Wipperman MF, Sampson NS, Thomas ST.	Crit Rev Biochem Mol Biol	10.3109/10409238.2014.895700	2014
Metabolism	Cloning and characterization of benzoate catabolic genes in the gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1.	Kitagawa W, Miyauchi K, Masai E, Fukuda M.	J Bacteriol	10.1128/jb.183.22.6598-6606.2001	2001
	A comparative study of some strains received as nocardiae.	GORDON RE, MIHM JM.	J Bacteriol	10.1128/jb.73.1.15-27.1957	1957
Enzymology	Efficient Synthesis of Steroidal Intermediates with a C17 Side Chain from Phytosterols by Genetically Modified Mycolicibacterium neoaurum NRRL B-3805 Strain.	Li X, Zhu L, Wu Q, Zhang R, Liu Y, Liu N, Feng J, Wu Q, Zhu D.	ChemistryOpen	10.1002/open.202500086	2025
Enzymology	Purification and characterization of a novel nitrilase of Rhodococcus rhodochrous K22 that acts on aliphatic nitriles.	Kobayashi M, Yanaka N, Nagasawa T, Yamada H.	J Bacteriol	10.1128/jb.172.9.4807-4815.1990	1990
Enzymology	Identification of a new class of cytochrome P450 from a Rhodococcus sp.	Roberts GA, Grogan G, Greter A, Flitsch SL, Turner NJ.	J Bacteriol	10.1128/jb.184.14.3898-3908.2002	2002
Enzymology	Distribution of aldoxime dehydratase in microorganisms.	Kato Y, Ooi R, Asano Y.	Appl Environ Microbiol	10.1128/aem.66.6.2290-2296.2000	2000
Enzymology	Application of 2,4-Dinitrophenylhydrazine (DNPH) in High-Throughput Screening for Microorganism Mutants Accumulating 9alpha-Hydroxyandrost-4-ene-3,17-dione (9alpha-OH-AD).	Liu Y, Cao F, Xiong H, Shen Y, Wang M	PLoS One	10.1371/journal.pone.0163836	2016
Enzymology	The effect of 3-ketosteroid-Delta(1)-dehydrogenase isoenzymes on the transformation of AD to 9alpha-OH-AD by Rhodococcus rhodochrous DSM43269.	Liu Y, Shen Y, Qiao Y, Su L, Li C, Wang M	J Ind Microbiol Biotechnol	10.1007/s10295-016-1804-0	2016
Enzymology	Substrate specificities and conformational flexibility of 3-ketosteroid 9alpha-hydroxylases.	Penfield JS, Worrall LJ, Strynadka NC, Eltis LD	J Biol Chem	10.1074/jbc.M114.575886	2014
Metabolism	Molecular characterization of ltp3 and ltp4, essential for C24-branched chain sterol-side-chain degradation in Rhodococcus rhodochrous DSM 43269.	Wilbrink MH, van der Geize R, Dijkhuizen L	Microbiology (Reading)	10.1099/mic.0.059501-0	2012
Enzymology	Structural features in the KshA terminal oxygenase protein that determine substrate preference of 3-ketosteroid 9alpha-hydroxylase enzymes.	Petrusma M, Dijkhuizen L, van der Geize R	J Bacteriol	10.1128/JB.05838-11	2011
Metabolism	Multiplicity of 3-Ketosteroid-9alpha-Hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids.	Petrusma M, Hessels G, Dijkhuizen L, van der Geize R	J Bacteriol	10.1128/JB.00274-11	2011
Metabolism	FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains.	Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R	Appl Environ Microbiol	10.1128/AEM.00380-11	2011
Metabolism	Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9alpha-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity.	Petrusma M, Dijkhuizen L, van der Geize R	Appl Environ Microbiol	10.1128/AEM.00066-09	2009
Metabolism	Characterization of Rrh4273I, a restriction-modification system of Rhodococcus rhodochrous ATCC 4273 (Nocardia corallina) which recognizes the same sequence as the Streptomyces albus G SalI restriction-modification system.	Yebra MJ, Novella IS, Barbes C, Aparicio JF, Martin CG, Hardisson C, Sanchez J	J Gen Microbiol	10.1099/00221287-137-6-1279	1991

References

#10801	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43269
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#35702	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#122228	Collection of Institut Pasteur ; Curators of the CIP; CIP 104623
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Rhodococcus rhodochrous (DSM 43269, ATCC 4273, CBS 197.57)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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