Name: Rhodococcus rhodochrous DSM 43269
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 10904

Type strain:

Species: Rhodococcus rhodochrous

Culture col. no.: DSM 43269, ATCC 4273, CBS 197.57, CCM 3245, CIP 104623, IFO 3338, NBRC 3338, NCIB 9259, CCUG 18001, JCM 3010, BCRC 11075, CECT 3046, CECT 3091, CGMCC 4.1480, IAM 12121, ICPB 4421, IFM 0025, IMET 7018, LMG 4077, NCCB 91001, NCIMB 9259, RIA 628, VKM Ac-860

Strain history: CIP <- 1995, NCIMB <- R.E. Gordon <- ATCC <- H.J. Conn, Mycobacterium agreste <- P.H.H. Gray: strain 0-3, Nocardia corallina

NCBI tax ID(s): 1829 (species)

SI-ID 92710

LMG 4077, ATCC 4273, CCM 3245, CECT 3046, IAM 12121, IFO 3338, IMET 7018, NCIB 9259, ATCC 21664, CCUG 18001, CIP 104623, NCIMB 9259, JCM 3010, CCRC 11075, VKM Ac-860, CBS 197.57, CECT 3091, NBRC 3338, NCCB 91001, LMD 91.1, BCRC 11075, NBIMCC 1359, CGMCC 4.1073, CGMCC 4.1480, DSM 43269

Special Collections

Other strains from Rhodococcus rhodochrous

Section

Rhodococcus rhodochrous DSM 43269 is an obligate aerobe, mesophilic, Gram-positive bacterium of the family Nocardiaceae.

Gram-positive
rod-shaped
mesophilic
obligate aerobe
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Nocardiaceae*
	Genus	*Rhodococcus*
	Species	**Rhodococcus rhodochrous**
	Full Scientific Name (LPSN)	Rhodococcus rhodochrous (Zopf 1891) Tsukamura 1974 (Approved Lists 1980)

	Synonym	Rhodococcus roseus
	Synonym	"Staphylococcus* rhodochrous"*

Information on morphological and physiological properties Morphology

[Ref.: #122228]	Gram stain	positive

[Ref.: #122228]	Cell shape	rod-shaped

[Ref.: #122228]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #10801]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #10801]

Culture medium growth

[Ref.: #10801]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10801]

Culture medium composition

expand / minimize

[Ref.: #35702]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #35702]

Culture medium growth

[Ref.: #35702]

Culture medium composition

expand / minimize

[Ref.: #122228]

Culture medium

CIP Medium 3

[Ref.: #122228]

Culture medium growth

[Ref.: #122228]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #10801]		growth	28 °C
[Ref.: #35702]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #122228]		growth	10-41 °C
[Ref.: #122228]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122228]

Oxygen tolerance

obligate aerobe

[Ref.: #10801]

Name of produced compound

restriction endonuclease Rrh4372I

	Salt	Tested relation		Salt conc.
[Ref.: #122228]	NaCl		growth	0-6	%
[Ref.: #122228]	NaCl	no	growth	8	%
[Ref.: #122228]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #122228]	citrate ChEBI	-	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #122228]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #122228]	nitrate ChEBI	-	builds gas from
[Ref.: #122228]	nitrate ChEBI	-	respiration
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #122228]	nitrite ChEBI	+	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122228]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #122228]	acetoin ChEBI	-
[Ref.: #122228]	glucose ChEBI		-
[Ref.: #68369]	indole ChEBI			-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #122228]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122228]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #122228]	beta-galactosidase	+/-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122228]	caseinase	-	3.4.21.50
[Ref.: #122228]	catalase	+	1.11.1.6
[Ref.: #122228]	DNase	-
[Ref.: #122228]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122228]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #122228]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #122228]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #122228]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #122228]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122228]	tryptophan deaminase	-
[Ref.: #122228]	tween esterase	+
[Ref.: #122228]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #10801]	27903	+	-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	+/-	-	+/-	+	-	-	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #122228]	739	not determinedn.d.	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	-	-	-	-	+	-	-	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #10801]	14707	-	-	-	-	-	+	+/-	-	-	-	-	-	-	-	-	+	+	-	-	-
[Ref.: #122228]	8084	-	-	+	+	+	+	+	+	-	-	+	+	-	-	-	+	+	-	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #10801]	Pathogenicity (human)	yes, in single cases
[Ref.: #10801]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122228]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

[Ref.: #67770]	GC-content	68.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	66 mol%	fluorimetric

Availability in culture collections External links

[Ref.: #10801]

Culture collection no.

DSM 43269, ATCC 4273, CBS 197.57, CCM 3245, CIP 104623, IFO 3338, NBRC 3338, NCIB 9259, CCUG 18001, JCM 3010, BCRC 11075, CECT 3046, CECT 3091, CGMCC 4.1480, IAM 12121, ICPB 4421, IFM 0025, IMET 7018, LMG 4077, NCCB 91001, NCIMB 9259, RIA 628, VKM Ac-860

[Ref.: #80159]

SI-ID 92710

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Application of 2,4-Dinitrophenylhydrazine (DNPH) in High-Throughput Screening for Microorganism Mutants Accumulating 9alpha-Hydroxyandrost-4-ene-3,17-dione (9alpha-OH-AD).	Liu Y, Cao F, Xiong H, Shen Y, Wang M	PLoS One	10.1371/journal.pone.0163836	2016	*
Enzymology	The effect of 3-ketosteroid-Delta(1)-dehydrogenase isoenzymes on the transformation of AD to 9alpha-OH-AD by Rhodococcus rhodochrous DSM43269.	Liu Y, Shen Y, Qiao Y, Su L, Li C, Wang M	J Ind Microbiol Biotechnol	10.1007/s10295-016-1804-0	2016	*
Enzymology	Substrate specificities and conformational flexibility of 3-ketosteroid 9alpha-hydroxylases.	Penfield JS, Worrall LJ, Strynadka NC, Eltis LD	J Biol Chem	10.1074/jbc.M114.575886	2014	*
Metabolism	Molecular characterization of ltp3 and ltp4, essential for C24-branched chain sterol-side-chain degradation in Rhodococcus rhodochrous DSM 43269.	Wilbrink MH, van der Geize R, Dijkhuizen L	Microbiology (Reading)	10.1099/mic.0.059501-0	2012	*
Enzymology	Structural features in the KshA terminal oxygenase protein that determine substrate preference of 3-ketosteroid 9alpha-hydroxylase enzymes.	Petrusma M, Dijkhuizen L, van der Geize R	J Bacteriol	10.1128/JB.05838-11	2011	*
Metabolism	Multiplicity of 3-Ketosteroid-9alpha-Hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids.	Petrusma M, Hessels G, Dijkhuizen L, van der Geize R	J Bacteriol	10.1128/JB.00274-11	2011	*
Metabolism	FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains.	Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R	Appl Environ Microbiol	10.1128/AEM.00380-11	2011	*
Metabolism	Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9alpha-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity.	Petrusma M, Dijkhuizen L, van der Geize R	Appl Environ Microbiol	10.1128/AEM.00066-09	2009	*
Metabolism	Characterization of Rrh4273I, a restriction-modification system of Rhodococcus rhodochrous ATCC 4273 (Nocardia corallina) which recognizes the same sequence as the Streptomyces albus G SalI restriction-modification system.	Yebra MJ, Novella IS, Barbes C, Aparicio JF, Martin CG, Hardisson C, Sanchez J	J Gen Microbiol	10.1099/00221287-137-6-1279	1991	*

References

#10801

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43269

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#35702 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#80159

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92710.1 )

#122228 Collection of Institut Pasteur ; Curators of the CIP; CIP 104623
* These data were automatically processed and therefore are not curated

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