Name: Nocardia callitridis DSM 45353
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 10825

Type strain:

Species: Nocardia callitridis

Culture col. no.: DSM 45353, ACM 5287, JCM 18298, CAP 290

Strain history: DSM 45353 <-- C. Franco CAP 290 <-- O. Kaewkla.

NCBI tax ID(s): 648753 (species)

SI-ID 397068

DSM 45353

Section

Nocardia callitridis DSM 45353 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from Australian native pine tree, Callitris preissii.

Gram-positive
ovoid-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Nocardiaceae*
	Genus	*Nocardia*
	Species	**Nocardia callitridis**
	Full Scientific Name (LPSN)	Nocardia callitridis Kaewkla and Franco 2010

Information on morphological and physiological properties Morphology

[Ref.: #29582]	Gram stain	positive

[Ref.: #29582]	Cell length	0.8 µm

[Ref.: #29582]	Cell width	0.7 µm

[Ref.: #29582]	Cell shape	ovoid-shaped

[Ref.: #29582]	Motility	no

[Ref.: #69384]	Colony color	Cream (9001)
[Ref.: #69384]	Cultivation medium used	ISP 4

[Ref.: #69384]	Colony color	Colourless
[Ref.: #69384]	Cultivation medium used	ISP 3

[Ref.: #69384]	Colony color	Colourless
[Ref.: #69384]	Cultivation medium used	ISP 6

[Ref.: #69384]	Colony color	Light ivory (1015)
[Ref.: #69384]	Cultivation medium used	ISP 2

[Ref.: #69384]	Colony color	Light ivory (1015)
[Ref.: #69384]	Cultivation medium used	ISP 5

[Ref.: #69384]	Colony color	Light ivory (1015)
[Ref.: #69384]	Cultivation medium used	ISP 7

[Ref.: #69384]	Colony color	Light ivory (1015)
[Ref.: #69384]	Cultivation medium used	suter with tyrosine

[Ref.: #69384]	Colony color	Light ivory (1015)
[Ref.: #69384]	Cultivation medium used	suter without tyrosine

[Ref.: #29582]	Pigment production	yes
[Ref.: #69384]	Pigment production	no
[Ref.: #69384]	Pigment name	Melanin
[Ref.: #69384]	Pigment production	no
[Ref.: #69384]	Pigment name	soluble pigment

[Ref.: #69384]	Multicellular complex forming ability	yes
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Multicellular complex color	Pure white (9010)
[Ref.: #69384]	Medium Name (multicellularity)	ISP 2

[Ref.: #69384]	Multicellular complex forming ability	yes
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Multicellular complex color	Pure white (9010)
[Ref.: #69384]	Medium Name (multicellularity)	ISP 3

[Ref.: #69384]	Multicellular complex forming ability	no
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Medium Name (multicellularity)	ISP 4

[Ref.: #69384]	Multicellular complex forming ability	yes
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Multicellular complex color	Cream (9001)
[Ref.: #69384]	Medium Name (multicellularity)	ISP 5

[Ref.: #69384]	Multicellular complex forming ability	yes
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Multicellular complex color	Cream (9001)
[Ref.: #69384]	Medium Name (multicellularity)	ISP 6

[Ref.: #69384]	Multicellular complex forming ability	yes
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Multicellular complex color	Cream (9001)
[Ref.: #69384]	Medium Name (multicellularity)	ISP 7

[Ref.: #69384]	Multicellular complex forming ability	no
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69384]	Multicellular complex forming ability	no
[Ref.: #69384]	Multicellular complex name	Aerial mycelium
[Ref.: #69384]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #16308]

Caption:

Medium 65 28°C

[Ref.: #16308]

Intellectual property rights:

[Ref.: #69384]

Caption:

Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69384]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69384]

Caption:

Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69384]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16308]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #16308]

Culture medium growth

[Ref.: #16308]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16308]

Culture medium composition

expand / minimize

[Ref.: #16308]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #16308]

Culture medium growth

[Ref.: #16308]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16308]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16308]	growth	28 °C
[Ref.: #29582]	growth	15-27 °C
[Ref.: #29582]	optimum	27 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #16308]	Temperature range	mesophilic
[Ref.: #29582]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29582]			optimum	8
[Ref.: #29582]			growth	05-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29582]

Oxygen tolerance

aerobe

[Ref.: #29582]

Ability of spore formation

yes

	Salt		Salt conc.
[Ref.: #29582]	NaCl	growth	0-15	%
[Ref.: #29582]	NaCl	optimum	0-5	%
[Ref.: #69384]	NaCl	growth	0-5	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄, ω-cycl)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #69384]	arabinose ChEBI	-	growth
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #69384]	cellulose ChEBI	-	growth
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE, API Coryne
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #29582]	fructose ChEBI	+	carbon source
[Ref.: #69384]	fructose ChEBI	-	growth
[Ref.: #29582]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE, API Coryne
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #69384]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69384]	inositol ChEBI	-	growth
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69384]	mannose ChEBI	-	growth
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE, API Coryne
[Ref.: #29582]	raffinose ChEBI	+	carbon source
[Ref.: #69384]	raffinose ChEBI	-	growth
[Ref.: #69384]	rhamnose ChEBI	-	growth
[Ref.: #29582]	ribose ChEBI	+	carbon source
[Ref.: #29582]	sorbitol ChEBI	+	carbon source
[Ref.: #69384]	sucrose ChEBI	-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29582]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API Coryne
[Ref.: #29582]	xylose ChEBI	+	carbon source
[Ref.: #69384]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #29582]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne, API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #16308]	25093	-	-	-	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69384]	3877	-	-	-	-	-	-	+/-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #16308]	14240	-	-	+	+/-	-	+	-	-	-	-	+/-	-	-	-	-	-	+	-	-	-
[Ref.: #69384]	6061	not determinedn.d.	+/-	+/-	+	-	+	+/-	-	-	-	+	+/-	-	+/-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16308]	Sample type/isolated from	Australian native pine tree, Callitris preissii
[Ref.: #16308]	Host species	Callitris preissii
[Ref.: #16308]	Geographic location (country and/or sea, region)	Adelaide, Flinders University campus
[Ref.: #16308]	Country	Australia
[Ref.: #16308]	Country ISO 3 Code	AUS
[Ref.: #16308]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Surface-sterilized root of a native pine tree (Callitris preissii) on the Flinders University campus
[Ref.: #67770]	Host species	Callitris preissii
[Ref.: #67770]	Geographic location (country and/or sea, region)	Adelaide, South Australia
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Tree

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16308]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16308]	Nocardia callitridis strain CAP 290 16S ribosomal RNA gene, partial sequence	FJ805428	1386	ENA	648753 tax ID

[Ref.: #16308]	GC-content	68.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29582]	GC-content	68.7 mol%

Availability in culture collections External links

[Ref.: #16308]

Culture collection no.

DSM 45353, ACM 5287, JCM 18298, CAP 290

[Ref.: #80086]

SI-ID 397068

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardia endophytica sp. nov., an endophytic actinomycete isolated from the oil-seed plant Jatropha curcas L.	Xing K, Qin S, Fei SM, Lin Q, Bian GK, Miao Q, Wang Y, Cao CL, Tang SK, Jiang JH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.027391-0	2010	*
Phylogeny	Nocardia callitridis sp. nov., an endophytic actinobacterium isolated from a surface-sterilized root of an Australian native pine tree.	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijs.0.016337-0	2009	*

References

#16308

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45353

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29582

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25974 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69384

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#80086

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397068.1 )

#25974

IJSEM 1532 2010 ( DOI 10.1099/ijs.0.016337-0 , PubMed 19684327 )

* These data were automatically processed and therefore are not curated

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