Name: Millisia brevis J81
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 10626

Type strain:

Species: Millisia brevis

Strain Designation: J81

Culture col. no.: DSM 44463, CIP 109234, NRRL B-24424, JCM 13999, BCRC 16840, IFM 10694, NBRC 105863

Strain history: CIP <- 2006, DSMZ <- M. Goodfellow, Newcastle Univ., Newcastle, UK: strain J81

NCBI tax ID(s): 1219361 (strain), 264148 (species)

SI-ID 215522

DSM 44463, NRRL B-24424, JCM 13999, CIP 109234, BCRC 16840, CCRC 16840

Section

Millisia brevis J81 is an aerobe, mesophilic, Gram-variable bacterium that was isolated from activated sludge.

Gram-variable
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Nocardiaceae*
	Genus	*Millisia*
	Species	**Millisia brevis**
	Full Scientific Name (LPSN)	Millisia brevis Soddell et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31586]	Gram stain	variable
[Ref.: #117045]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31586]	Cell shape	rod-shaped
[Ref.: #117045]	Cell shape	rod-shaped

[Ref.: #31586]	Motility	no
[Ref.: #117045]	Motility	no

[Ref.: #19864]	Colony color	Salmon orange
[Ref.: #19864]	Incubation period	10-14 days
[Ref.: #19864]	Cultivation medium used	ISP 2

[Ref.: #19864]	Colony color	Salmon orange
[Ref.: #19864]	Incubation period	10-14 days
[Ref.: #19864]	Cultivation medium used	ISP 3

[Ref.: #19864]	Colony color	Salmon orange
[Ref.: #19864]	Incubation period	10-14 days
[Ref.: #19864]	Cultivation medium used	ISP 4

[Ref.: #19864]	Colony color	Salmon orange
[Ref.: #19864]	Incubation period	10-14 days
[Ref.: #19864]	Cultivation medium used	ISP 5

[Ref.: #19864]	Colony color	Salmon orange
[Ref.: #19864]	Incubation period	10-14 days
[Ref.: #19864]	Cultivation medium used	ISP 6

[Ref.: #19864]	Colony color	Salmon orange
[Ref.: #19864]	Incubation period	10-14 days
[Ref.: #19864]	Cultivation medium used	ISP 7

[Ref.: #31586]

Pigment production

yes

[Ref.: #19864]	Multicellular complex forming ability	no
[Ref.: #19864]	Medium Name (multicellularity)	ISP 2

[Ref.: #19864]	Multicellular complex forming ability	no
[Ref.: #19864]	Medium Name (multicellularity)	ISP 3

[Ref.: #19864]	Multicellular complex forming ability	no
[Ref.: #19864]	Medium Name (multicellularity)	ISP 4

[Ref.: #19864]	Multicellular complex forming ability	no
[Ref.: #19864]	Medium Name (multicellularity)	ISP 5

[Ref.: #19864]	Multicellular complex forming ability	no
[Ref.: #19864]	Medium Name (multicellularity)	ISP 6

[Ref.: #19864]	Multicellular complex forming ability	no
[Ref.: #19864]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11790]

Culture medium

BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b)

[Ref.: #11790]

Culture medium growth

[Ref.: #11790]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11790]

Culture medium composition

expand / minimize

[Ref.: #19864]

Culture medium

ISP 2

[Ref.: #19864]

Culture medium growth

[Ref.: #19864]

Culture medium composition

expand / minimize

[Ref.: #19864]

Culture medium

ISP 3

[Ref.: #19864]

Culture medium growth

[Ref.: #19864]

Culture medium composition

expand / minimize

[Ref.: #19864]

Culture medium

ISP 4

[Ref.: #19864]

Culture medium growth

[Ref.: #19864]

Culture medium composition

expand / minimize

[Ref.: #19864]

Culture medium

ISP 5

[Ref.: #19864]

Culture medium growth

[Ref.: #19864]

Culture medium composition

expand / minimize

[Ref.: #19864]

Culture medium

ISP 6

[Ref.: #19864]

Culture medium growth

[Ref.: #19864]

Culture medium composition

expand / minimize

[Ref.: #19864]

Culture medium

ISP 7

[Ref.: #19864]

Culture medium growth

[Ref.: #19864]

Culture medium composition

expand / minimize

[Ref.: #37487]

Culture medium

MEDIUM 58 - for Nocardia asteroides and Nocardioides luteus

[Ref.: #37487]

Culture medium growth

[Ref.: #37487]

Culture medium composition

expand / minimize

[Ref.: #117045]

Culture medium

CIP Medium 253

[Ref.: #117045]

Culture medium growth

[Ref.: #117045]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #11790]	growth	28 °C
[Ref.: #19864]	optimum	28 °C
[Ref.: #31586]	growth	15-35 °C
[Ref.: #31586]	optimum	30-35 °C
[Ref.: #37487]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #11790]	Temperature range	mesophilic
[Ref.: #19864]	Temperature range	mesophilic
[Ref.: #31586]	Temperature range	mesophilic
[Ref.: #37487]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31586]			optimum	6.5-7.5
[Ref.: #31586]			growth	5.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31586]	Oxygen tolerance	aerobe
[Ref.: #117045]	Oxygen tolerance	obligate aerobe

[Ref.: #31586]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.366

[Ref.: #31586]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #31586]	cellobiose ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE, API Coryne
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #31586]	ethanol ChEBI	+	carbon source
[Ref.: #31586]	fructose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE, API Coryne
[Ref.: #31586]	glucose ChEBI	+	carbon source
[Ref.: #31586]	glycerol ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31586]	mannitol ChEBI	+	carbon source
[Ref.: #31586]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #117045]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE, API Coryne
[Ref.: #117045]	nitrite ChEBI	-	reduction
[Ref.: #31586]	sucrose ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31586]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #117045]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #31586]	catalase	+	1.11.1.6
[Ref.: #117045]	catalase	+	1.11.1.6
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #117045]	oxidase	-
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #31586]	urease	+	3.5.1.5
[Ref.: #117045]	urease	+	3.5.1.5
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #11790]	25088	-	-	-	-	+	-	-	-	+	+/-	+/-	+	-	+/-	+/-	-	+/-	+	+/-	+/-	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #19864]	352	-	-	-	-	-	-	+	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19864]	676	not determinedn.d.	-	+	+	-	+	+	+	+	-	-	+	-	-	-	+	+	-	+	-
[Ref.: #11790]	14235	-	+	-	+/-	-	+	+	+/-	-	-	+	+/-	-	-	-	+	+	-	+/-	-
[Ref.: #117045]	11735	-	-	+	+	-	+	+	-	+	-	+	-	-	-	-	+	+	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11790]	Sample type/isolated from	activated sludge
[Ref.: #11790]	Geographic location (country and/or sea, region)	Victoria, Bendigo
[Ref.: #11790]	Country	Australia
[Ref.: #11790]	Country ISO 3 Code	AUS
[Ref.: #11790]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Activated sludge foam from Tamworth Sewage Treatment Plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	New South Wales
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania

[Ref.: #117045]	Sample type/isolated from	Environment, Activated sludge
[Ref.: #117045]	Country	Australia
[Ref.: #117045]	Country ISO 3 Code	AUS
[Ref.: #117045]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY534742 (>99% sequence identity) for Millisia brevis subclade from Microbeatlas

Aquatic Samples	2643
Soil Samples	961
Animal Samples	1712
Plant Samples	122
Total Samples	5438

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11790]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19864]	Biosafety level	1	Risk group
[Ref.: #117045]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11790]	Millisia brevis strain J81 16S ribosomal RNA gene, partial sequence	AY534742	1519	ENA	264148 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Millisia brevis NBRC 105863	GCA_001552615	contig	GenBank	1219361 tax ID
[Ref.: #66792]	Millisia brevis NBRC 105863	1219361.4	wgs	PATRIC	1219361 tax ID	*
[Ref.: #66792]	Millisia brevis NBRC 105863	2731957593	draft	JGI IMG/M	1219361 tax ID	*

[Ref.: #31586]	GC-content	64.7 mol%
[Ref.: #67770]	GC-content	64.7 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Millisia brevis NBRC 105863 PATRIC: 1219361.4
[Ref.: #69481]	spore-forming	no	64	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Millisia brevis NBRC 105863 PATRIC: 1219361.4
[Ref.: #69480]	motile	no	94.94	no
[Ref.: #69480]	gram-positive	yes	78.15	yes
[Ref.: #69480]	anaerobic	no	99.277	yes
[Ref.: #69480]	aerobic	yes	89.004	yes
[Ref.: #69480]	halophile	no	91.503	no
[Ref.: #69480]	spore-forming	no	86.758	yes
[Ref.: #69480]	thermophile	no	99.671	yes
[Ref.: #69480]	glucose-util	yes	88.793	yes
[Ref.: #69480]	flagellated	no	98.682	no
[Ref.: #69480]	glucose-ferment	no	90.781	yes

Availability in culture collections External links

[Ref.: #11790]

Culture collection no.

DSM 44463, CIP 109234, NRRL B-24424, JCM 13999, BCRC 16840, IFM 10694, NBRC 105863

[Ref.: #79898]

SI-ID 215522

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam.	Soddell JA, Stainsby FM, Eales KL, Kroppenstedt RM, Seviour RJ, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijs.0.63855-0	2006	*

References

#11790

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44463

#19864

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31586

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27875 (see below)

#37487 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#79898

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID215522.1 )

#117045 Collection of Institut Pasteur ; Curators of the CIP; CIP 109234

#27875

IJSEM 739 2006 ( DOI 10.1099/ijs.0.63855-0 , PubMed 16585686 )

* These data were automatically processed and therefore are not curated

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Search for species Millisia brevis in external resources:
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Millisia brevis NBRC 105863

Millisia brevis NBRC 105863

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