Strain identifier

BacDive ID: 10626

Type strain: Yes

Species: Millisia brevis

Strain Designation: J81

Strain history: CIP <- 2006, DSMZ <- M. Goodfellow, Newcastle Univ., Newcastle, UK: strain J81

NCBI tax ID(s): 1219361 (strain), 264148 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 11790

BacDive-ID: 10626

DSM-Number: 44463

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-variable, rod-shaped

description: Millisia brevis J81 is an aerobe, mesophilic, Gram-variable bacterium that was isolated from activated sludge.

NCBI tax id

NCBI tax idMatching level
1219361strain
264148species

strain history

@refhistory
11790<- M. Goodfellow, Univ. Newcastle; J81
67770IFM 10694 <-- DSM 44463 <-- M. Goodfellow J81.
117045CIP <- 2006, DSMZ <- M. Goodfellow, Newcastle Univ., Newcastle, UK: strain J81

doi: 10.13145/bacdive10626.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Millisia
  • species: Millisia brevis
  • full scientific name: Millisia brevis Soddell et al. 2006

@ref: 11790

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Millisia

species: Millisia brevis

full scientific name: Millisia brevis Soddell et al. 2006

strain designation: J81

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31586variablerod-shapedno
69480positive100
117045positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
19864Salmon orange10-14 daysISP 2
19864Salmon orange10-14 daysISP 3
19864Salmon orange10-14 daysISP 4
19864Salmon orange10-14 daysISP 5
19864Salmon orange10-14 daysISP 6
19864Salmon orange10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19864noISP 2
19864noISP 3
19864noISP 4
19864noISP 5
19864noISP 6
19864noISP 7

pigmentation

  • @ref: 31586
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11790BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b)yeshttps://mediadive.dsmz.de/medium/215bName: BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b) Composition: Brain heart infusion 37.0 g/l Glucose 5.0 g/l Distilled water
19864ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19864ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19864ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19864ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19864ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19864ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37487MEDIUM 58 - for Nocardia asteroides and Nocardioides luteusyesDistilled water make up to (1000.000 ml);Glucose (5.000 g);Brain heart infusion (37.000 g)
117045CIP Medium 253yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=253

culture temp

@refgrowthtypetemperaturerange
19864positiveoptimum28mesophilic
11790positivegrowth28mesophilic
31586positivegrowth15-35
31586positiveoptimum30-35mesophilic
37487positivegrowth30mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
31586positivegrowth5.5-9.5alkaliphile
31586positiveoptimum6.5-7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31586aerobe
117045obligate aerobe

spore formation

@refspore formationconfidence
31586no
69480no99.366

observation

@refobservation
31586aggregates in chains
67770quinones: MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3158617057cellobiose+carbon source
3158616236ethanol+carbon source
3158628757fructose+carbon source
3158617234glucose+carbon source
3158617754glycerol+carbon source
3158629864mannitol+carbon source
3158637684mannose+carbon source
3158617992sucrose+carbon source
3158627082trehalose+carbon source
6836925115malate+assimilation
6836927689decanoate-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
11704517632nitrate-reduction
11704516301nitrite-reduction
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11704535581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
31586catalase+1.11.1.6
31586urease+3.5.1.5
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
117045oxidase-
117045catalase+1.11.1.6
117045urease+3.5.1.5
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382alpha-chymotrypsin-3.4.21.1
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19864------+-+----------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19864-++-++++--+---++-+-
11790+-+/--+++/---++/----++-+/---
117045-++-++-+-+----++-+--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
11790----+---++/-+/-+-+/-+/--+/-++/-+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11790activated sludgeVictoria, BendigoAustraliaAUSAustralia and Oceania
67770Activated sludge foam from Tamworth Sewage Treatment PlantNew South WalesAustraliaAUSAustralia and Oceania
117045Environment, Activated sludgeAustraliaAUSAustralia and Oceania

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Activated sludge

taxonmaps

  • @ref: 69479
  • File name: preview.99_6435.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_3044;97_3741;98_4749;99_6435&stattab=map
  • Last taxonomy: Millisia brevis subclade
  • 16S sequence: AY534742
  • Sequence Identity:
  • Total samples: 5438
  • soil counts: 961
  • aquatic counts: 2643
  • animal counts: 1712
  • plant counts: 122

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
117901Risk group (German classification)
198641Risk group
1170451Risk group (French classification)

Sequence information

16S sequences

  • @ref: 11790
  • description: Millisia brevis strain J81 16S ribosomal RNA gene, partial sequence
  • accession: AY534742
  • length: 1519
  • database: ena
  • NCBI tax ID: 264148

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Millisia brevis NBRC 1058631219361.4wgspatric1219361
66792Millisia brevis NBRC 1058632731957593draftimg1219361
67770Millisia brevis NBRC 105863GCA_001552615contigncbi1219361

GC content

@refGC-contentmethod
3158664.7
6777064.7high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno64no
motileno94.94no
gram-positiveyes78.15yes
anaerobicno99.277yes
aerobicyes89.004yes
halophileno91.503no
spore-formingno86.758yes
thermophileno99.671yes
glucose-utilyes88.793yes
flagellatedno98.682no
glucose-fermentno90.781yes

External links

@ref: 11790

culture collection no.: DSM 44463, CIP 109234, NRRL B-24424, JCM 13999, BCRC 16840, IFM 10694, NBRC 105863

straininfo link

  • @ref: 79898
  • straininfo: 215522

literature

  • topic: Phylogeny
  • Pubmed-ID: 16585686
  • title: Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam.
  • authors: Soddell JA, Stainsby FM, Eales KL, Kroppenstedt RM, Seviour RJ, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63855-0
  • year: 2006
  • mesh: Actinobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Cell Wall/chemistry, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis/genetics, Sewage/*microbiology, Waste Disposal, Fluid
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
11790Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44463)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44463
19864Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44463.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31586Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2787528776041
37487Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6953
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
79898Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID215522.1StrainInfo: A central database for resolving microbial strain identifiers
117045Curators of the CIPCollection of Institut Pasteur (CIP 109234)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109234