Gordonia neofelifaecis AD-6 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from faeces of a clouded leopard .
Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
|
|
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Gordoniaceae |
| Genus Gordonia |
| Species Gordonia neofelifaecis |
| Full scientific name Gordonia neofelifaecis Liu et al. 2011 |
| @ref | Colony color | Incubation period | Medium used | |
|---|---|---|---|---|
| 21403 | sand yellow (1002) | 10-14 days | ISP 2 | |
| 21403 | 10-14 days | ISP 3 | ||
| 21403 | 10-14 days | ISP 4 | ||
| 21403 | 10-14 days | ISP 5 | ||
| 21403 | Light ivory (1015) | 10-14 days | ISP 7 | |
| 21403 | Sand yellow (1002) – Brown beige (1011) | 10-14 days | Suter with tyrosine | |
| 21403 | Ivory (1015) | 10-14 days | Suter without tyrosine |
| @ref: | 17966 |
| multimedia content: | DSM_45646.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_45646.jpg |
| caption: | Medium 65 28°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 17966 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 21403 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 21403 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 21403 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 21403 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 21403 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 21403 | Suter with tyrosine | Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes | |||
| 21403 | Suter without tyrosine | Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes |
| 29762 | Spore formationno |
| @ref | Salt | Growth | Tested relation | Concentration | |
|---|---|---|---|---|---|
| 21403 | NaCl | positive | maximum | 10 % |
| 67770 | Observationquinones: MK-9(H2) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 21403 | 22599 ChEBI | arabinose | - | ||
| 21403 | 62968 ChEBI | cellulose | + | ||
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 21403 | 28757 ChEBI | fructose | + | ||
| 29762 | 28260 ChEBI | galactose | + | carbon source | |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 21403 | 17234 ChEBI | glucose | + | ||
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 29762 | 25017 ChEBI | leucine | + | carbon source | |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 21403 | 37684 ChEBI | mannose | - | ||
| 21403 | 17268 ChEBI | myo-inositol | - | ||
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 21403 | 16634 ChEBI | raffinose | - | ||
| 21403 | 26546 ChEBI | rhamnose | - | ||
| 29762 | 26546 ChEBI | rhamnose | + | carbon source | |
| 29762 | 17822 ChEBI | serine | + | carbon source | |
| 21403 | 17992 ChEBI | sucrose | + | ||
| 68379 | 17992 ChEBI | sucrose | + | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | + | hydrolysis | from API Coryne |
| 21403 | 18222 ChEBI | xylose | + |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 68382 | cystine arylamidase | + | 3.4.11.3 | from API zym |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 68379 | gelatinase | - | from API Coryne | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | + | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 68379 | urease | + | 3.5.1.5 | from API Coryne |
| 68382 | valine arylamidase | + | from API zym |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Host | #Mammals | #Felidae (Cat) | |
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
Global distribution of 16S sequence FJ938167 (>99% sequence identity) for Gordonia from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM19243v1 assembly for Gordonia neofelifaecis NRRL B-59395 | contig | 644548 | 69.4 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 17966 | Gordonia neofelifaecis NRRL B-59395 strain AD-6 16S ribosomal RNA gene, partial sequence | FJ938167 | 1417 | 644548 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 92.73 | yes |
| 125438 | anaerobic | anaerobicⓘ | no | 97.30 | yes |
| 125438 | aerobic | aerobicⓘ | yes | 91.67 | no |
| 125438 | spore-forming | spore-formingⓘ | no | 61.02 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 97.99 | no |
| 125438 | flagellated | motile2+ⓘ | no | 95.10 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Genetics | Genomic, Phylogenetic and Physiological Characterization of the PAH-Degrading Strain Gordonia polyisoprenivorans 135. | Frantsuzova E, Bogun A, Kopylova O, Vetrova A, Solyanikova I, Streletskii R, Delegan Y. | Biology (Basel) | 10.3390/biology13050339 | 2024 | |
| Efficient biotransformation of cholesterol to androsta-1,4-diene-3,17-dione by a newly isolated actinomycete Gordonia neofelifaecis | Liu Y, Chen G, Ge F, Li W, Zeng L, Cao W. | World J Microbiol Biotechnol | 10.1007/s11274-010-0513-5 | 2011 | ||
| Genetics | Whole Genome Analysis and Assessment of the Metabolic Potential of Gordonia rubripertincta Strain 112, a Degrader of Aromatic and Aliphatic Compounds. | Frantsuzova E, Bogun A, Solomentsev V, Vetrova A, Streletskii R, Solyanikova I, Delegan Y. | Biology (Basel) | 10.3390/biology12050721 | 2023 | |
| Transcriptome | Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666T Strain. | Lobastova T, Fokina V, Pozdnyakova-Filatova I, Tarlachkov S, Shutov A, Donova M. | Int J Mol Sci | 10.3390/ijms232416174 | 2022 | |
| Metabolism | Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate. | Shtratnikova VY, Sshelkunov MI, Fokina VV, Bragin EY, Shutov AA, Donova MV. | BMC Biotechnol | 10.1186/s12896-021-00668-9 | 2021 | |
| Isolation and characterization of a new highly effective 17beta-estradiol-degrading Gordonia sp. strain R9. | Liu N, Shi YE, Li J, Zhu M, Zhang T. | 3 Biotech | 10.1007/s13205-020-2156-z | 2020 | ||
| Metabolism | Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9alpha-hydroxylated derivatives by engineered Mycobacteria. | Li X, Chen T, Peng F, Song S, Yu J, Sidoine DN, Cheng X, Huang Y, He Y, Su Z. | Microb Cell Fact | 10.1186/s12934-021-01653-9 | 2021 | |
| Metabolism | Steroids as Environmental Compounds Recalcitrant to Degradation: Genetic Mechanisms of Bacterial Biodegradation Pathways. | Olivera ER, Luengo JM. | Genes (Basel) | 10.3390/genes10070512 | 2019 | |
| New Insights on Steroid Biotechnology. | Fernandez-Cabezon L, Galan B, Garcia JL. | Front Microbiol | 10.3389/fmicb.2018.00958 | 2018 | ||
| Genetics | Characterization and Genomic Analysis of a Highly Efficient Dibutyl Phthalate-Degrading Bacterium Gordonia sp. Strain QH-12. | Jin D, Kong X, Liu H, Wang X, Deng Y, Jia M, Yu X. | Int J Mol Sci | 10.3390/ijms17071012 | 2016 | |
| Metabolism | Testosterone Degradative Pathway of Novosphingobium tardaugens. | Ibero J, Galan B, Diaz E, Garcia JL. | Genes (Basel) | 10.3390/genes10110871 | 2019 | |
| Transcriptome | Genome-wide response on phytosterol in 9-hydroxyandrostenedione-producing strain of Mycobacterium sp. VKM Ac-1817D. | Bragin EY, Shtratnikova VY, Schelkunov MI, Dovbnya DV, Donova MV. | BMC Biotechnol | 10.1186/s12896-019-0533-7 | 2019 | |
| Metabolism | Genome-Wide Transcriptome Profiling of Mycobacterium smegmatis MC² 155 Cultivated in Minimal Media Supplemented with Cholesterol, Androstenedione or Glycerol. | Li Q, Ge F, Tan Y, Zhang G, Li W. | Int J Mol Sci | 10.3390/ijms17050689 | 2016 | |
| Metabolism | Cholesterol degradation by Gordonia cholesterolivorans. | Drzyzga O, Fernandez de las Heras L, Morales V, Navarro Llorens JM, Perera J. | Appl Environ Microbiol | 10.1128/aem.05149-11 | 2011 | |
| Diverse alkane hydroxylase genes in microorganisms and environments. | Nie Y, Chi CQ, Fang H, Liang JL, Lu SL, Lai GL, Tang YQ, Wu XL. | Sci Rep | 10.1038/srep04968 | 2014 | ||
| Bacteriophages of Gordonia spp. Display a Spectrum of Diversity and Genetic Relationships. | Pope WH, Mavrich TN, Garlena RA, Guerrero-Bustamante CA, Jacobs-Sera D, Montgomery MT, Russell DA, Warner MH, Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES), Hatfull GF. | mBio | 10.1128/mbio.01069-17 | 2017 | ||
| Metabolism | The genome of the moderate halophile Amycolicicoccus subflavus DQS3-9A1(T) reveals four alkane hydroxylation systems and provides some clues on the genetic basis for its adaptation to a petroleum environment. | Nie Y, Fang H, Li Y, Chi CQ, Tang YQ, Wu XL. | PLoS One | 10.1371/journal.pone.0070986 | 2013 | |
| Metabolism | Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms. | Szaleniec M, Wojtkiewicz AM, Bernhardt R, Borowski T, Donova M. | Appl Microbiol Biotechnol | 10.1007/s00253-018-9239-3 | 2018 | |
| Genetics | Genome-Based Taxonomic Classification of the Phylum Actinobacteria. | Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M. | Front Microbiol | 10.3389/fmicb.2018.02007 | 2018 | |
| Metabolism | [Accumulation of 9alpha-hydroxy-4-androstene-3,17-dione by co-expressing kshA and kshB encoding component of 3-ketosteroid-9alpha-hydroxylase in Mycobacterium sp. NRRL B-3805]. | Yuan J, Chen G, Cheng S, Ge F, Qiong W, Li W, Li J | Sheng Wu Gong Cheng Xue Bao | 2015 | ||
| Metabolism | Identification of gene expression profiles in the actinomycete Gordonia neofelifaecis grown with different steroids. | Li W, Ge F, Zhang Q, Ren Y, Yuan J, He J, Li W, Chen G, Zhang G, Zhuang Y, Xu L | Genome | 10.1139/gen-2014-0030 | 2014 | |
| Genetics | Draft genome sequence of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete. | Ge F, Li W, Chen G, Liu Y, Zhang G, Yong B, Wang Q, Wang N, Huang Z, Li W, Wang J, Wu C, Xie Q, Liu G | J Bacteriol | 10.1128/JB.05531-11 | 2011 | |
| Phylogeny | Gordonia crocea sp. nov. and Gordonia spumicola sp. nov. isolated from sludge of a wastewater treatment plant. | Tamura T, Saito S, Hamada M, Kang Y, Hoshino Y, Gonoi T, Mikami Y, Yaguchi T | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004225 | 2020 | |
| Phylogeny | Gordonia neofelifaecis sp. nov., a cholesterol side-chain-cleaving actinomycete isolated from the faeces of Neofelis nebulosa. | Liu Y, Ge F, Chen G, Li W, Ma P, Zhang G, Zeng L | Int J Syst Evol Microbiol | 10.1099/ijs.0.020321-0 | 2010 |
| #17966 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45646 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #21403 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #26143 | IJSEM 165 2011 ( DOI 10.1099/ijs.0.020321-0 , PubMed 20190025 ) |
| #29762 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26143 |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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